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The map label for this gene is brnQ [H]

Identifier: 218903062

GI number: 218903062

Start: 1854372

End: 1855730

Strand: Direct

Name: brnQ [H]

Synonym: BCAH820_1945

Alternate gene names: 218903062

Gene position: 1854372-1855730 (Clockwise)

Preceding gene: 218903061

Following gene: 218903063

Centisome position: 34.97

GC content: 36.04

Gene sequence:

>1359_bases
ATGACGAATAAAGTACCGTTTTCGTTCATAGTTGTTATTGGTTTAATGTTATTTGCACTATTTTTTGGGGCAGGAAATTT
AATATTCCCAGCAATGCTTGGTCAATCGGCAGGAGAGAATGTGTGGATTGCTAACGCTGGATTTTTAGTAACAGGTGTAG
GTTTACCGTTGCTAGGGGTATTAGCATTTGGAATTTCAGGTAAAGAAGATTTACAATCGTTAGCAAGTCGTGTACATCCA
GTGTTCGGCATTGTATTTACGACAGTTTTATATTTAGCGATTGGTCCACTATTCGCTATTCCGAGAACAGGCAGCGTTTC
TTATGAAATTGGTATGAAGCCTTTTGTACCAGAAGCTATGAGTTCTTCAGCACTCATTCTTTTTACAATTATATTCTTTA
GCATCACTTGTTTTTTTTCGCTAAACCCCGCGAAAATTGTCGATATTGTAGGAAAAATCTTAACGCCAATTAAGTTAACG
TTTATTGGGATTTTAGTAGTATTTGCTTTTATTCATCCAATGGGAGAAATGCAAGCACCTGCAGAGGCATATACATCACA
TGCGTTCTTTAAAGGGTTCCAAGAAGGTTACTTAACAATGGATACGTTAGCATCATTCGTATTCGGTATTATTATCATCA
WTGCGATTAAAGAAAAGGGCGCAAAAACAAAAAAAGACATTATGATTGTTTGTGCAAAAGCAACATTWATTGCTGCATCT
ATTTTAGCAATGATCTATTCAGCACTTTCATTTATGGGAGCTTCAAGTGTTGCAAAACTTGGTCATTTAGATAATGGTGG
TGCAGTATTAGCAAAAGTTTCAAACTATTATTTTGGGTCATACGGCGGAATTATATTAGGAATTATGGTTACAGCAGCAT
GTTTAACGACTAGTGTAGGACTTGTTTCAGCGTGTTCTTCATTCTTCCATAAACTATTCCCGAATGTTTCGTATAAAAAG
ATAGCAATCATCTTATCAGTGTTCAGTGCAGTTGTTGCGAATGTAGGTTTAACACAATTAATCGCAATTTCTGTTCCTGT
ATTAATCGCGATTTATCCATTAGCAATCGTCTTAATTTTCCTAACGCTCCTTCATCCATTGTTTAAAGGGCGAGCAGAAG
TGTATCAAGGTAGCTTATTAATAACATTTATTGTAAGTATGTTCGATGGATTAAATGCAGCTGGTATGAAGTTAGCAGTA
GTGAACGATTTTCTTACTAAAATACTCCCAATGCATGAAGTTGGATTAGGATGGATTTTACCAGCACTTATTGGTGGATT
GATCGGATACGTCATTAGCATTGTGAAAGCAAAAAATCAAGTTCAACCAGCAGCTAGTGCGGATAAGAAAATAGGTTAA

Upstream 100 bases:

>100_bases
TAATGAGTTCAAGAATAGTTGATACTTAAGTTAAATATTCAGAAAATTCAATTAGTTAAAATTGGAAATGACATAGGTTA
TTTTGAATGGAGGTTATATT

Downstream 100 bases:

>100_bases
ATAAAAAAGTTATAGAACTTGTTTCTATAACTTTTTTTGTTACTATTAATAATGAGAGAAAATAAAGTAGTAGGGGAAAT
AAAATGAAAAAAACAATTGA

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BrnQ [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MTNKVPFSFIVVIGLMLFALFFGAGNLIFPAMLGQSAGENVWIANAGFLVTGVGLPLLGVLAFGISGKEDLQSLASRVHP
VFGIVFTTVLYLAIGPLFAIPRTGSVSYEIGMKPFVPEAMSSSALILFTIIFFSITCFFSLNPAKIVDIVGKILTPIKLT
FIGILVVFAFIHPMGEMQAPAEAYTSHAFFKGFQEGYLTMDTLASFVFGIIIIXAIKEKGAKTKKDIMIVCAKATXIAAS
ILAMIYSALSFMGASSVAKLGHLDNGGAVLAKVSNYYFGSYGGIILGIMVTAACLTTSVGLVSACSSFFHKLFPNVSYKK
IAIILSVFSAVVANVGLTQLIAISVPVLIAIYPLAIVLIFLTLLHPLFKGRAEVYQGSLLITFIVSMFDGLNAAGMKLAV
VNDFLTKILPMHEVGLGWILPALIGGLIGYVISIVKAKNQVQPAASADKKIG

Sequences:

>Translated_452_residues
MTNKVPFSFIVVIGLMLFALFFGAGNLIFPAMLGQSAGENVWIANAGFLVTGVGLPLLGVLAFGISGKEDLQSLASRVHP
VFGIVFTTVLYLAIGPLFAIPRTGSVSYEIGMKPFVPEAMSSSALILFTIIFFSITCFFSLNPAKIVDIVGKILTPIKLT
FIGILVVFAFIHPMGEMQAPAEAYTSHAFFKGFQEGYLTMDTLASFVFGIIIIXAIKEKGAKTKKDIMIVCAKATXIAAS
ILAMIYSALSFMGASSVAKLGHLDNGGAVLAKVSNYYFGSYGGIILGIMVTAACLTTSVGLVSACSSFFHKLFPNVSYKK
IAIILSVFSAVVANVGLTQLIAISVPVLIAIYPLAIVLIFLTLLHPLFKGRAEVYQGSLLITFIVSMFDGLNAAGMKLAV
VNDFLTKILPMHEVGLGWILPALIGGLIGYVISIVKAKNQVQPAASADKKIG
>Mature_451_residues
TNKVPFSFIVVIGLMLFALFFGAGNLIFPAMLGQSAGENVWIANAGFLVTGVGLPLLGVLAFGISGKEDLQSLASRVHPV
FGIVFTTVLYLAIGPLFAIPRTGSVSYEIGMKPFVPEAMSSSALILFTIIFFSITCFFSLNPAKIVDIVGKILTPIKLTF
IGILVVFAFIHPMGEMQAPAEAYTSHAFFKGFQEGYLTMDTLASFVFGIIIIXAIKEKGAKTKKDIMIVCAKATXIAASI
LAMIYSALSFMGASSVAKLGHLDNGGAVLAKVSNYYFGSYGGIILGIMVTAACLTTSVGLVSACSSFFHKLFPNVSYKKI
AIILSVFSAVVANVGLTQLIAISVPVLIAIYPLAIVLIFLTLLHPLFKGRAEVYQGSLLITFIVSMFDGLNAAGMKLAVV
NDFLTKILPMHEVGLGWILPALIGGLIGYVISIVKAKNQVQPAASADKKIG

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=427, Percent_Identity=39.5784543325527, Blast_Score=296, Evalue=2e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 47822; Mature: 47691

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKVPFSFIVVIGLMLFALFFGAGNLIFPAMLGQSAGENVWIANAGFLVTGVGLPLLGV
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHH
LAFGISGKEDLQSLASRVHPVFGIVFTTVLYLAIGPLFAIPRTGSVSYEIGMKPFVPEAM
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHH
SSSALILFTIIFFSITCFFSLNPAKIVDIVGKILTPIKLTFIGILVVFAFIHPMGEMQAP
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AEAYTSHAFFKGFQEGYLTMDTLASFVFGIIIIXAIKEKGAKTKKDIMIVCAKATXIAAS
HHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHH
ILAMIYSALSFMGASSVAKLGHLDNGGAVLAKVSNYYFGSYGGIILGIMVTAACLTTSVG
HHHHHHHHHHHHCHHHHHHHCCCCCCCCEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LVSACSSFFHKLFPNVSYKKIAIILSVFSAVVANVGLTQLIAISVPVLIAIYPLAIVLIF
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LTLLHPLFKGRAEVYQGSLLITFIVSMFDGLNAAGMKLAVVNDFLTKILPMHEVGLGWIL
HHHHHHHHCCHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
PALIGGLIGYVISIVKAKNQVQPAASADKKIG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TNKVPFSFIVVIGLMLFALFFGAGNLIFPAMLGQSAGENVWIANAGFLVTGVGLPLLGV
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHH
LAFGISGKEDLQSLASRVHPVFGIVFTTVLYLAIGPLFAIPRTGSVSYEIGMKPFVPEAM
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHH
SSSALILFTIIFFSITCFFSLNPAKIVDIVGKILTPIKLTFIGILVVFAFIHPMGEMQAP
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AEAYTSHAFFKGFQEGYLTMDTLASFVFGIIIIXAIKEKGAKTKKDIMIVCAKATXIAAS
HHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHH
ILAMIYSALSFMGASSVAKLGHLDNGGAVLAKVSNYYFGSYGGIILGIMVTAACLTTSVG
HHHHHHHHHHHHCHHHHHHHCCCCCCCCEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LVSACSSFFHKLFPNVSYKKIAIILSVFSAVVANVGLTQLIAISVPVLIAIYPLAIVLIF
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LTLLHPLFKGRAEVYQGSLLITFIVSMFDGLNAAGMKLAVVNDFLTKILPMHEVGLGWIL
HHHHHHHHCCHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
PALIGGLIGYVISIVKAKNQVQPAASADKKIG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023181; 9308178; 9384377 [H]