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The map label for this gene is vanS [H]

Identifier: 218903030

GI number: 218903030

Start: 1816548

End: 1817414

Strand: Direct

Name: vanS [H]

Synonym: BCAH820_1913

Alternate gene names: 218903030

Gene position: 1816548-1817414 (Clockwise)

Preceding gene: 218903029

Following gene: 218903031

Centisome position: 34.26

GC content: 32.06

Gene sequence:

>867_bases
ATGACAAAAACAATGATGAAAAGATTGTCTGAAATTAATAAGAACGTGAAGGAAATAAGTAAGGGTAATTTTGAAGTATA
CATACCTATTTCAAAACATGACGAGATTGGTGAATTAGCGACGAATGTAAATAGAATGGCCAAAAGTTTAAAAGAGTCTG
TCGAGAATGAAAAAAAATCACAGGAAATGAAAAATGAAATGATTAGTAATATTTCGCATGACTTACGAACACCTGTAACA
TCTTTAATCGGTTATGCGGACTTATTAGGAAATAAGCTGCATGCAAATGGAGAAGAATGTGAACAGTATGTAAGCATATT
AAAGCGAAAAAGTTATGAATTAAAAAATCAAGTAGATGAATTATTAGAATACTGTCAAATAAATTATAGAGAGATTGAAT
TACATAAAAGTGTAGTAAATATGAAAGCGTTAATGGAACAAATTATGATTGATTTTGTACCACAACTAGATGATGCTAAT
ATGAGCTTTTGTATAAAAGGTGATAAGGAGTTACATGTGGAAATAGATGTAGCGCTTATGGTGAGGTTATTCGAAAATGT
AATTAGTAATAGTATTATGTACGGGAAAGACGGAAAGGAGATTTTAATTCTAGTTTCTAAAAGAGACATGAATGTTGAAA
TAGAAGTTAAAAATTTTGGACAATGTATTCCAGATGAGAACCTTCCTTACGTTTTTGAGAAGTTTTATCGTGGTGAAAAA
TCACGTAGCTCCCATACAGGTGGAAAAGGAATGGGACTTGCAATTGCGAGAAGTATAGCAGAGCTTCATAAAGGGGATAT
CACTGTGCGCAGTAACGAGAAAGAAACTATTTTCACTATCGCTTTACCGCAGCATAAAGAATTGTAA

Upstream 100 bases:

>100_bases
AATTGGAAATATATATAGTCATGTTACTACGTTAGAGGAAAAATATTCATTTTTATATTTTATAGTTTTTTTGATTTTTA
CTACGGCATACTTTGCATGT

Downstream 100 bases:

>100_bases
GAAAGTCGTAAGTATTTCTTTTATATGTCGTAATTTTTAATTCGTATCATGTAGAACAAAGATATGAGAGTAGGGATACG
ACATGTGGAAGAAATATGTA

Product: sensory box histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MTKTMMKRLSEINKNVKEISKGNFEVYIPISKHDEIGELATNVNRMAKSLKESVENEKKSQEMKNEMISNISHDLRTPVT
SLIGYADLLGNKLHANGEECEQYVSILKRKSYELKNQVDELLEYCQINYREIELHKSVVNMKALMEQIMIDFVPQLDDAN
MSFCIKGDKELHVEIDVALMVRLFENVISNSIMYGKDGKEILILVSKRDMNVEIEVKNFGQCIPDENLPYVFEKFYRGEK
SRSSHTGGKGMGLAIARSIAELHKGDITVRSNEKETIFTIALPQHKEL

Sequences:

>Translated_288_residues
MTKTMMKRLSEINKNVKEISKGNFEVYIPISKHDEIGELATNVNRMAKSLKESVENEKKSQEMKNEMISNISHDLRTPVT
SLIGYADLLGNKLHANGEECEQYVSILKRKSYELKNQVDELLEYCQINYREIELHKSVVNMKALMEQIMIDFVPQLDDAN
MSFCIKGDKELHVEIDVALMVRLFENVISNSIMYGKDGKEILILVSKRDMNVEIEVKNFGQCIPDENLPYVFEKFYRGEK
SRSSHTGGKGMGLAIARSIAELHKGDITVRSNEKETIFTIALPQHKEL
>Mature_287_residues
TKTMMKRLSEINKNVKEISKGNFEVYIPISKHDEIGELATNVNRMAKSLKESVENEKKSQEMKNEMISNISHDLRTPVTS
LIGYADLLGNKLHANGEECEQYVSILKRKSYELKNQVDELLEYCQINYREIELHKSVVNMKALMEQIMIDFVPQLDDANM
SFCIKGDKELHVEIDVALMVRLFENVISNSIMYGKDGKEILILVSKRDMNVEIEVKNFGQCIPDENLPYVFEKFYRGEKS
RSSHTGGKGMGLAIARSIAELHKGDITVRSNEKETIFTIALPQHKEL

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=287, Percent_Identity=25.7839721254355, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1786783, Length=276, Percent_Identity=25.3623188405797, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786912, Length=237, Percent_Identity=27.0042194092827, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786600, Length=222, Percent_Identity=25.6756756756757, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI145693157, Length=242, Percent_Identity=23.9669421487603, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082128, Length=235, Percent_Identity=25.531914893617, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789149, Length=256, Percent_Identity=23.4375, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787894, Length=236, Percent_Identity=26.271186440678, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI48994928, Length=235, Percent_Identity=27.2340425531915, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788279, Length=264, Percent_Identity=25.3787878787879, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790436, Length=242, Percent_Identity=24.3801652892562, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790861, Length=242, Percent_Identity=22.3140495867769, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790346, Length=275, Percent_Identity=21.8181818181818, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 32995; Mature: 32863

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTMMKRLSEINKNVKEISKGNFEVYIPISKHDEIGELATNVNRMAKSLKESVENEKKS
CCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QEMKNEMISNISHDLRTPVTSLIGYADLLGNKLHANGEECEQYVSILKRKSYELKNQVDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LLEYCQINYREIELHKSVVNMKALMEQIMIDFVPQLDDANMSFCIKGDKELHVEIDVALM
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEHHHHHH
VRLFENVISNSIMYGKDGKEILILVSKRDMNVEIEVKNFGQCIPDENLPYVFEKFYRGEK
HHHHHHHHHCCEEECCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCCC
SRSSHTGGKGMGLAIARSIAELHKGDITVRSNEKETIFTIALPQHKEL
CCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
TKTMMKRLSEINKNVKEISKGNFEVYIPISKHDEIGELATNVNRMAKSLKESVENEKKS
CHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QEMKNEMISNISHDLRTPVTSLIGYADLLGNKLHANGEECEQYVSILKRKSYELKNQVDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LLEYCQINYREIELHKSVVNMKALMEQIMIDFVPQLDDANMSFCIKGDKELHVEIDVALM
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEHHHHHH
VRLFENVISNSIMYGKDGKEILILVSKRDMNVEIEVKNFGQCIPDENLPYVFEKFYRGEK
HHHHHHHHHCCEEECCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCCC
SRSSHTGGKGMGLAIARSIAELHKGDITVRSNEKETIFTIALPQHKEL
CCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]