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The map label for this gene is dapG1 [H]

Identifier: 218902974

GI number: 218902974

Start: 1753840

End: 1755069

Strand: Reverse

Name: dapG1 [H]

Synonym: BCAH820_1857

Alternate gene names: 218902974

Gene position: 1755069-1753840 (Counterclockwise)

Preceding gene: 218902978

Following gene: 218902973

Centisome position: 33.1

GC content: 37.24

Gene sequence:

>1230_bases
ATGGAAACGATTGTACAAAAATTTGGTGGCACTTCTGTCGGAAGCGTTGAACGCATTCAACATGTAGCAAATTTAATTAT
TGAAGAATATGAACGAGGACATAGTATCGTCTCTGTCGTTTCAGCAATGGGGAAAAGTACAGACGAACTTGTAGCACTTG
CTAACGCGATTACAGAAAATCCGAGTAAACGCGAAATGGATATGCTTCTATCAACAGGAGAGCAAGTCACTATTTCATTA
TTAACGATGGCATTACAAGCAAAAGGTTATAACGCAATTTCATTAACAGGATGGCAAGCTGGTATTACGACAGAATCTGT
ACATAGTAGTGCACGGATTACTGAAATTAATACAGATCGAATTCAGTCTTACCTTACTAAAGGCAAGATTGTTATTGTAG
CTGGTTTCCAAGGAGTTAGTGAAGACCTTGAAATTACAACGCTTGGACGTGGTGGTTCTGATACAACTGCTGTTGCATTA
GCTGCGGCACTGAACGCAAAAAAATGTGATATTTACACAGATGTGACCGGCGTATATACGACGGATCCACGAGTTGTAAA
AGATGCTTATAAATTAGATGAAATTTCTTATGACGAAATGTTAGAACTTGCAAATCTCGGTGCTGGTGTATTACACCCGC
GTGCTGTTGAGTTTGCTAAAAATCATAATGTCATTTTAGAAGTTCGCTCAAGTATGGAACAAGAAAATGGAACAATTGTA
AAAGGAGAATGTAACATGGAACAACAATCAATCGTTAAAGGTATTGCATTTGAAGATAATATTACACGCATCACAGTGAA
AGGATTGGAACAAGGATCGCTTTCAACTGTTTTCTCTACATTAGCAGCGGCACATATTAATGTAGACATCATCATTCAAA
GTATTACAAATGAAGGAACTGTTCATCTCTCCTTCTCCATCCATTCTAATGATTTAAGAGAAACGTTAGAAGTGTTGGAA
CAAAATCAAGAGGCACTTCACTATGAATCAGTAGAATATGAAAATCATTTAGCAAAAGTATCAATTGTAGGATCTGGTAT
GGTCTCTAATCCAGGTGTCGCTGCGAATATGTTCACTACTTTAAAAGAAGAAGATATTCATATTAAAATGGTAAGTACAT
CAGAAATTAAAGTGTCTGTCGTTATTGACCGCCTTCATTTAGTAACAGGTGTCGAGGCGCTGCATCAATCATTTATGGCG
AAAATTGAGCCTTTAGTGCAGATGAGTTAA

Upstream 100 bases:

>100_bases
TGAGATGGTGTTTTTTGTTTTTCTCTGGTAACTACAGGAATATTGGGATTTATATATGTCCTAATCTATTCTTAAAAATG
GATGAAAGAAGGAATTCGTA

Downstream 100 bases:

>100_bases
TTTCTACTAGTAAAACTTAAAACAAATTATATGAATCTACTTCCTTCCTATATAAATAGAAAGCTATTTGCATATAGAGT
AATAAGAAATGTATTTATCA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase 2; Aspartokinase II [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
METIVQKFGGTSVGSVERIQHVANLIIEEYERGHSIVSVVSAMGKSTDELVALANAITENPSKREMDMLLSTGEQVTISL
LTMALQAKGYNAISLTGWQAGITTESVHSSARITEINTDRIQSYLTKGKIVIVAGFQGVSEDLEITTLGRGGSDTTAVAL
AAALNAKKCDIYTDVTGVYTTDPRVVKDAYKLDEISYDEMLELANLGAGVLHPRAVEFAKNHNVILEVRSSMEQENGTIV
KGECNMEQQSIVKGIAFEDNITRITVKGLEQGSLSTVFSTLAAAHINVDIIIQSITNEGTVHLSFSIHSNDLRETLEVLE
QNQEALHYESVEYENHLAKVSIVGSGMVSNPGVAANMFTTLKEEDIHIKMVSTSEIKVSVVIDRLHLVTGVEALHQSFMA
KIEPLVQMS

Sequences:

>Translated_409_residues
METIVQKFGGTSVGSVERIQHVANLIIEEYERGHSIVSVVSAMGKSTDELVALANAITENPSKREMDMLLSTGEQVTISL
LTMALQAKGYNAISLTGWQAGITTESVHSSARITEINTDRIQSYLTKGKIVIVAGFQGVSEDLEITTLGRGGSDTTAVAL
AAALNAKKCDIYTDVTGVYTTDPRVVKDAYKLDEISYDEMLELANLGAGVLHPRAVEFAKNHNVILEVRSSMEQENGTIV
KGECNMEQQSIVKGIAFEDNITRITVKGLEQGSLSTVFSTLAAAHINVDIIIQSITNEGTVHLSFSIHSNDLRETLEVLE
QNQEALHYESVEYENHLAKVSIVGSGMVSNPGVAANMFTTLKEEDIHIKMVSTSEIKVSVVIDRLHLVTGVEALHQSFMA
KIEPLVQMS
>Mature_409_residues
METIVQKFGGTSVGSVERIQHVANLIIEEYERGHSIVSVVSAMGKSTDELVALANAITENPSKREMDMLLSTGEQVTISL
LTMALQAKGYNAISLTGWQAGITTESVHSSARITEINTDRIQSYLTKGKIVIVAGFQGVSEDLEITTLGRGGSDTTAVAL
AAALNAKKCDIYTDVTGVYTTDPRVVKDAYKLDEISYDEMLELANLGAGVLHPRAVEFAKNHNVILEVRSSMEQENGTIV
KGECNMEQQSIVKGIAFEDNITRITVKGLEQGSLSTVFSTLAAAHINVDIIIQSITNEGTVHLSFSIHSNDLRETLEVLE
QNQEALHYESVEYENHLAKVSIVGSGMVSNPGVAANMFTTLKEEDIHIKMVSTSEIKVSVVIDRLHLVTGVEALHQSFMA
KIEPLVQMS

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=342, Percent_Identity=32.4561403508772, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1790455, Length=251, Percent_Identity=37.4501992031873, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1790376, Length=459, Percent_Identity=23.7472766884532, Blast_Score=89, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6320893, Length=377, Percent_Identity=26.790450928382, Blast_Score=115, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 44393; Mature: 44393

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METIVQKFGGTSVGSVERIQHVANLIIEEYERGHSIVSVVSAMGKSTDELVALANAITEN
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCC
PSKREMDMLLSTGEQVTISLLTMALQAKGYNAISLTGWQAGITTESVHSSARITEINTDR
CCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHCCCCEEEECCHHH
IQSYLTKGKIVIVAGFQGVSEDLEITTLGRGGSDTTAVALAAALNAKKCDIYTDVTGVYT
HHHHHHCCCEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEECCCEEEE
TDPRVVKDAYKLDEISYDEMLELANLGAGVLHPRAVEFAKNHNVILEVRSSMEQENGTIV
CCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEEEHHHHHHCCCCEE
KGECNMEQQSIVKGIAFEDNITRITVKGLEQGSLSTVFSTLAAAHINVDIIIQSITNEGT
EEECCCCHHHHHHCCEECCCCEEEEEECCCCCCHHHHHHHHHHHHCEEEEEEEEECCCCE
VHLSFSIHSNDLRETLEVLEQNQEALHYESVEYENHLAKVSIVGSGMVSNPGVAANMFTT
EEEEEEECCHHHHHHHHHHHCCCHHHHHCCCCHHCCEEEEEEEECCCCCCCCCHHHHHHH
LKEEDIHIKMVSTSEIKVSVVIDRLHLVTGVEALHQSFMAKIEPLVQMS
HCCCCEEEEEEECCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
METIVQKFGGTSVGSVERIQHVANLIIEEYERGHSIVSVVSAMGKSTDELVALANAITEN
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCC
PSKREMDMLLSTGEQVTISLLTMALQAKGYNAISLTGWQAGITTESVHSSARITEINTDR
CCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHCCCCEEEECCHHH
IQSYLTKGKIVIVAGFQGVSEDLEITTLGRGGSDTTAVALAAALNAKKCDIYTDVTGVYT
HHHHHHCCCEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEECCCEEEE
TDPRVVKDAYKLDEISYDEMLELANLGAGVLHPRAVEFAKNHNVILEVRSSMEQENGTIV
CCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEEEHHHHHHCCCCEE
KGECNMEQQSIVKGIAFEDNITRITVKGLEQGSLSTVFSTLAAAHINVDIIIQSITNEGT
EEECCCCHHHHHHCCEECCCCEEEEEECCCCCCHHHHHHHHHHHHCEEEEEEEEECCCCE
VHLSFSIHSNDLRETLEVLEQNQEALHYESVEYENHLAKVSIVGSGMVSNPGVAANMFTT
EEEEEEECCHHHHHHHHHHHCCCHHHHHCCCCHHCCEEEEEEEECCCCCCCCCHHHHHHH
LKEEDIHIKMVSTSEIKVSVVIDRLHLVTGVEALHQSFMAKIEPLVQMS
HCCCCEEEEEEECCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1444390 [H]