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The map label for this gene is nirA [H]

Identifier: 218902632

GI number: 218902632

Start: 1431269

End: 1432891

Strand: Direct

Name: nirA [H]

Synonym: BCAH820_1515

Alternate gene names: 218902632

Gene position: 1431269-1432891 (Clockwise)

Preceding gene: 218902631

Following gene: 218902633

Centisome position: 26.99

GC content: 36.78

Gene sequence:

>1623_bases
ATGAGTTATGAAAAAGTATGGGCTAACAATGAAAAATTAAATCAAACTGAGAAAAATAAATTAAAAAAAGATGGATTGGA
AATCTTTAATGATATTCCTTACTATGCAGAGAATGGCTTCGAATCTATTCCGAAAGAAGAGTGGGACGCATTCAAATGGG
CAGGGTTGTATTTACAAAGACCGAAAGAAGCTGGTTATTTTATGATGCGCGTAAATATTCCTTCTGGGATTATTACGAAT
GCACAGGCAGAAGTGCTTGCTTCTATCGCTGAGGATTATGGACGTGACGTAATCGATATTACGACAAGACAGGCGATTCA
GTTTCATTGGTTAGAAATTCATCAAATTCCAGATATTTTTAAAAGATTAGCAAGAGTTGGGTTATCTTCAGCTGGAGCGT
GTGGTGATATAACGCGTAATATTACAGGAAATCCACTTGCTGGTATTGATGCAAATGAATTATTTGATACAACGCATATT
GTGAAAGAGGTATATGACTACTTCCAACATAATGAAGAGTTTTCTAACTTACCACGTAAATTTAAAATGTCTATTAGTTC
TAACATTTATAACGCAGCAAATGCAGAGATTAACTGTGTTGCATTTACACCTGCTACGAAAGAGATAGATGGTGAGAAAA
AAGTTGGTTTTCATATAAAAGTAGGTGGTGGGTTATCAGCTCGTCCATATTTAGCTGATGAATTAGATGTATTCGTTTTA
CCAGAAGAAGTGAAGGCGGTAGCGATTGCGATTGCTACGATATTCCGTGATTTTGGATATCGCGAAAAACGTCATTTAGC
TCGTTTGAAATTTCTTGTTGCAGACTGGGGAGCAGAGAAGTTTAAAGAGAAACTAATAGAGTATACAGGACCATTACAAA
GTAAAGGGGAAAGTGCTCTCAAAGGCTGGAATGCGGGATACTTTTATGGTGTCCAAGATCAAAAACAAGAGGGACTAAAA
TATGTAGGTTTTAATGTGCCAGTAGGGCGCCTACATGCAGAAGAAATGTTTGAGATTGCAAGAATTGCAAAACAATATGG
AAATGGACAAATTCGTACATGTAATTCGCAAAACTTCATTATTCCGAATGTTCCGCCGGAAAATGTAACGGGCTTACTAA
CTGAGCCGTTGTTTGAAGCGATATCAGCGAATCCGAAATCGTTTATTGGTCACGCGGTTTCTTGTACTGGTATTGAATAT
TGCAATCTTGCTTTAGTAGAAACGAAAGAAAGATTACGAAAAATTGCCGAATACTTAGATACACAAATTGCACTCGATGT
TCCGGTTCGTATTCATATGGTAGGATGTCCGAATTCGTGTGGTCAACGTCAAATTGCGGACATTGGATTACAAGGTGTGA
AAATGAAAACGAAGGAAAAAGGGATTGTTGAAGCATTTGAAATATATGTAGGTGGAACGTTGTTAGATGGTGGTGCTTAT
AATCAAAAGTTAAAAGGAAAAATAGATGGTGAGGATCTACCTGATGTACTCGCATCATTTATAAGTTATTTCAAAGAAAA
TAAATTACCGGCTGAAACGTTTTATGATTTTGTAGGCCGCGTTGGTGTAGAGACATTACAAATAGCGTTAAATAACGTGT
TAGAAGAAGTAATAGCGTCTTAG

Upstream 100 bases:

>100_bases
CGGAATTAATTGTAGAAACGCATAAGTATTCTATTGAAGAATGTGCAGAACAAATCGTGAAGTACTTACAAGAGCGTAGT
TTGATATAGGGGGATAAAAA

Downstream 100 bases:

>100_bases
GAGGGGCAATATGGATTTATATCGATTTGAAGCTGTATTAGAGAATAGTATTGTTCCAATTGTTGTTGTTGCTGAAAGTG
AAGAAAAGGCATTTAAGATG

Product: putative sulfite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MSYEKVWANNEKLNQTEKNKLKKDGLEIFNDIPYYAENGFESIPKEEWDAFKWAGLYLQRPKEAGYFMMRVNIPSGIITN
AQAEVLASIAEDYGRDVIDITTRQAIQFHWLEIHQIPDIFKRLARVGLSSAGACGDITRNITGNPLAGIDANELFDTTHI
VKEVYDYFQHNEEFSNLPRKFKMSISSNIYNAANAEINCVAFTPATKEIDGEKKVGFHIKVGGGLSARPYLADELDVFVL
PEEVKAVAIAIATIFRDFGYREKRHLARLKFLVADWGAEKFKEKLIEYTGPLQSKGESALKGWNAGYFYGVQDQKQEGLK
YVGFNVPVGRLHAEEMFEIARIAKQYGNGQIRTCNSQNFIIPNVPPENVTGLLTEPLFEAISANPKSFIGHAVSCTGIEY
CNLALVETKERLRKIAEYLDTQIALDVPVRIHMVGCPNSCGQRQIADIGLQGVKMKTKEKGIVEAFEIYVGGTLLDGGAY
NQKLKGKIDGEDLPDVLASFISYFKENKLPAETFYDFVGRVGVETLQIALNNVLEEVIAS

Sequences:

>Translated_540_residues
MSYEKVWANNEKLNQTEKNKLKKDGLEIFNDIPYYAENGFESIPKEEWDAFKWAGLYLQRPKEAGYFMMRVNIPSGIITN
AQAEVLASIAEDYGRDVIDITTRQAIQFHWLEIHQIPDIFKRLARVGLSSAGACGDITRNITGNPLAGIDANELFDTTHI
VKEVYDYFQHNEEFSNLPRKFKMSISSNIYNAANAEINCVAFTPATKEIDGEKKVGFHIKVGGGLSARPYLADELDVFVL
PEEVKAVAIAIATIFRDFGYREKRHLARLKFLVADWGAEKFKEKLIEYTGPLQSKGESALKGWNAGYFYGVQDQKQEGLK
YVGFNVPVGRLHAEEMFEIARIAKQYGNGQIRTCNSQNFIIPNVPPENVTGLLTEPLFEAISANPKSFIGHAVSCTGIEY
CNLALVETKERLRKIAEYLDTQIALDVPVRIHMVGCPNSCGQRQIADIGLQGVKMKTKEKGIVEAFEIYVGGTLLDGGAY
NQKLKGKIDGEDLPDVLASFISYFKENKLPAETFYDFVGRVGVETLQIALNNVLEEVIAS
>Mature_539_residues
SYEKVWANNEKLNQTEKNKLKKDGLEIFNDIPYYAENGFESIPKEEWDAFKWAGLYLQRPKEAGYFMMRVNIPSGIITNA
QAEVLASIAEDYGRDVIDITTRQAIQFHWLEIHQIPDIFKRLARVGLSSAGACGDITRNITGNPLAGIDANELFDTTHIV
KEVYDYFQHNEEFSNLPRKFKMSISSNIYNAANAEINCVAFTPATKEIDGEKKVGFHIKVGGGLSARPYLADELDVFVLP
EEVKAVAIAIATIFRDFGYREKRHLARLKFLVADWGAEKFKEKLIEYTGPLQSKGESALKGWNAGYFYGVQDQKQEGLKY
VGFNVPVGRLHAEEMFEIARIAKQYGNGQIRTCNSQNFIIPNVPPENVTGLLTEPLFEAISANPKSFIGHAVSCTGIEYC
NLALVETKERLRKIAEYLDTQIALDVPVRIHMVGCPNSCGQRQIADIGLQGVKMKTKEKGIVEAFEIYVGGTLLDGGAYN
QKLKGKIDGEDLPDVLASFISYFKENKLPAETFYDFVGRVGVETLQIALNNVLEEVIAS

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=512, Percent_Identity=25.5859375, Blast_Score=117, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322597, Length=379, Percent_Identity=26.1213720316623, Blast_Score=103, Evalue=8e-23,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.7.7.1 [H]

Molecular weight: Translated: 60432; Mature: 60300

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYEKVWANNEKLNQTEKNKLKKDGLEIFNDIPYYAENGFESIPKEEWDAFKWAGLYLQR
CCCCHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCHHHCCHHHHCHHHHHHHHEEC
PKEAGYFMMRVNIPSGIITNAQAEVLASIAEDYGRDVIDITTRQAIQFHWLEIHQIPDIF
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHH
KRLARVGLSSAGACGDITRNITGNPLAGIDANELFDTTHIVKEVYDYFQHNEEFSNLPRK
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCHH
FKMSISSNIYNAANAEINCVAFTPATKEIDGEKKVGFHIKVGGGLSARPYLADELDVFVL
EEHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEE
PEEVKAVAIAIATIFRDFGYREKRHLARLKFLVADWGAEKFKEKLIEYTGPLQSKGESAL
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHH
KGWNAGYFYGVQDQKQEGLKYVGFNVPVGRLHAEEMFEIARIAKQYGNGQIRTCNSQNFI
CCCCCCEEECCCHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEE
IPNVPPENVTGLLTEPLFEAISANPKSFIGHAVSCTGIEYCNLALVETKERLRKIAEYLD
ECCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCEECCHHHHCCHHHHHHHHHHHHHHHHC
TQIALDVPVRIHMVGCPNSCGQRQIADIGLQGVKMKTKEKGIVEAFEIYVGGTLLDGGAY
CEEEEECCEEEEEEECCCCCCCHHHHHHCCCCCEECCHHCCHHHHHHHEECCEEECCCCC
NQKLKGKIDGEDLPDVLASFISYFKENKLPAETFYDFVGRVGVETLQIALNNVLEEVIAS
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SYEKVWANNEKLNQTEKNKLKKDGLEIFNDIPYYAENGFESIPKEEWDAFKWAGLYLQR
CCCHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCHHHCCHHHHCHHHHHHHHEEC
PKEAGYFMMRVNIPSGIITNAQAEVLASIAEDYGRDVIDITTRQAIQFHWLEIHQIPDIF
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHH
KRLARVGLSSAGACGDITRNITGNPLAGIDANELFDTTHIVKEVYDYFQHNEEFSNLPRK
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCHH
FKMSISSNIYNAANAEINCVAFTPATKEIDGEKKVGFHIKVGGGLSARPYLADELDVFVL
EEHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEE
PEEVKAVAIAIATIFRDFGYREKRHLARLKFLVADWGAEKFKEKLIEYTGPLQSKGESAL
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHH
KGWNAGYFYGVQDQKQEGLKYVGFNVPVGRLHAEEMFEIARIAKQYGNGQIRTCNSQNFI
CCCCCCEEECCCHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEE
IPNVPPENVTGLLTEPLFEAISANPKSFIGHAVSCTGIEYCNLALVETKERLRKIAEYLD
ECCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCEECCHHHHCCHHHHHHHHHHHHHHHHC
TQIALDVPVRIHMVGCPNSCGQRQIADIGLQGVKMKTKEKGIVEAFEIYVGGTLLDGGAY
CEEEEECCEEEEEEECCCCCCCHHHHHHCCCCCEECCHHCCHHHHHHHEECCEEECCCCC
NQKLKGKIDGEDLPDVLASFISYFKENKLPAETFYDFVGRVGVETLQIALNNVLEEVIAS
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8490140 [H]