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The map label for this gene is ilvB [H]

Identifier: 218902605

GI number: 218902605

Start: 1405996

End: 1407705

Strand: Direct

Name: ilvB [H]

Synonym: BCAH820_1488

Alternate gene names: 218902605

Gene position: 1405996-1407705 (Clockwise)

Preceding gene: 218902604

Following gene: 218902606

Centisome position: 26.51

GC content: 39.94

Gene sequence:

>1710_bases
ATGAGAACAATGTCTTCAAAAACGGAAGAGAAACTGGCAACCGGTGCACAGCTATTGTTAGAAGCGCTAGAAAAGGAAGG
AGTAGAAGTGATTTTTGGTTATCCTGGTGGTGCAGTTTTACCTCTTTATGATGCGCTCTATGACTGTGAAATTCCGCATA
TTTTAACAAGGCATGAACAAGGAGCTATTCACGCCGCGGAAGGGTATGCCAGAATTACTGGCAATCCAGGGGTTGTCATT
GCAACGAGTGGACCAGGTGCTACGAATGTAATTACTGGTCTAGCAGATGCGATGATTGATTCATTGCCACTTGTTGTATT
TACAGGGCAAGTAGCAACAACGTTAATTGGAAGCGATGCTTTCCAAGAAGCGGATATTATGGGGCTTACGATGCCAGTGA
CAAAACATAACTATCAAGTGCGAAAGGCTTCAGATTTACCTCGAATTATTAAAGAAGCATTTCATATTGCTAGAACAGGA
AGACCAGGCCCAGTCGTCATTGACCTTCCGAAAGATATGGTAGTAGAGCAAGGCGAACGATGTAGTGATGTGCAAATGGA
TTTACCAGGATACAACCCTAATTATGAACCGAATCTACTGCAAATAAACAAATTATTACAAGCAATTCAGGTTGCAAACA
AGCCATTAATTTTAGCTGGAGCAGGTGTATTGCATGCGAAAGCTTCAAAAGAATTAACAAGTTTTGCTCGTAAATATGAA
ATTCCTGTTGTGCATACATTACTTGGTCTTGGTGGGTTTCCACCAGATGATGAACTATTTCTAGGGATGGGAGGAATGCA
TGGTTCTTACACAGCTAATATGGCGTTATATGAATGCGACTTACTCATTAATATAGGCGCGAGATTCGATGATCGTCTTA
CTGGAAATTTAGCTTATTTTGCGAAAGAGGCGACTGTCGCGCATATCGATATTGATCCAGCAGAAATCGGAAAAAATGTA
CCGACTGAAATTCCTATTGTTGCAAGTGCTAAGCAAGCGCTCCAAGTATTGCTTCAACCAGAAGGAAGGAAAGAAAATCA
TCATGAGTGGATCTCTTTATTAAAGAATAGAAAAGAAAAGTATCCATTCTCTTATAAACGAAATTCTGAAAGTATTAAGC
CTCAATATGCAATTGATATGTTATATGAAATTACGAAGGGAGAAGCGATTGTAACAACAGATGTGGGGCAACATCAAATG
TGGGCAGCACAATATTATCCGCTCAAAACTCCAGATAAATGGGTAACTTCAGGAGGACTAGGAACGATGGGATTTGGATT
TCCGGCAGCAATTGGTGCACAAATTGCAAAGCCTGATGAACTAGTTATTGCAATTGTCGGTGATGCTGGATTTCAAATGA
CTCTGCAAGAATTAAGTGTGTTAAAAGAACACTCTTTACCTGTTAAAGTATTTATTTTAAATAATGAAGCTTTAGGAATG
GTAAGACAATGGCAAGATGAATTTTATAACCAAAGATATTCGCACTCTTTACTATCGTGTCAACCAGATTTCGTTGCTCT
TGCGAATGCGTATGGCATAAAAGGGGTTCGCATAGATGATCCACTTCTTGCAAAGAAACAATTGCAGCATGCAATTCAAT
TACAAGAGCCAGTTGTAATTGATTGCCGTGTACTTCAATCAGAAAAGGTAATGCCGATGGTTGCGCCAGGGAAAGGTGTT
CACCAAATGGAGGGAGTGGAAAAAAGGTGA

Upstream 100 bases:

>100_bases
AAAGAAGGGTGGTACCGCGAAATCTTTCGTCCCTTCAGCACATAGCTGGAGTGTGGACGTAGGATTTTTTTTATTGATAA
AAGTTTTTAAAAGGGGGCTT

Downstream 100 bases:

>100_bases
AGAGAATTGTTACAGCAACAGTTCGAAATCAAAGTGGTGTGTTAAACCGAATTACAGGTGTTATGACACGAAGACATTTT
AATATTGAAAGTATTTCAGT

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 569; Mature: 569

Protein sequence:

>569_residues
MRTMSSKTEEKLATGAQLLLEALEKEGVEVIFGYPGGAVLPLYDALYDCEIPHILTRHEQGAIHAAEGYARITGNPGVVI
ATSGPGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDAFQEADIMGLTMPVTKHNYQVRKASDLPRIIKEAFHIARTG
RPGPVVIDLPKDMVVEQGERCSDVQMDLPGYNPNYEPNLLQINKLLQAIQVANKPLILAGAGVLHAKASKELTSFARKYE
IPVVHTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECDLLINIGARFDDRLTGNLAYFAKEATVAHIDIDPAEIGKNV
PTEIPIVASAKQALQVLLQPEGRKENHHEWISLLKNRKEKYPFSYKRNSESIKPQYAIDMLYEITKGEAIVTTDVGQHQM
WAAQYYPLKTPDKWVTSGGLGTMGFGFPAAIGAQIAKPDELVIAIVGDAGFQMTLQELSVLKEHSLPVKVFILNNEALGM
VRQWQDEFYNQRYSHSLLSCQPDFVALANAYGIKGVRIDDPLLAKKQLQHAIQLQEPVVIDCRVLQSEKVMPMVAPGKGV
HQMEGVEKR

Sequences:

>Translated_569_residues
MRTMSSKTEEKLATGAQLLLEALEKEGVEVIFGYPGGAVLPLYDALYDCEIPHILTRHEQGAIHAAEGYARITGNPGVVI
ATSGPGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDAFQEADIMGLTMPVTKHNYQVRKASDLPRIIKEAFHIARTG
RPGPVVIDLPKDMVVEQGERCSDVQMDLPGYNPNYEPNLLQINKLLQAIQVANKPLILAGAGVLHAKASKELTSFARKYE
IPVVHTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECDLLINIGARFDDRLTGNLAYFAKEATVAHIDIDPAEIGKNV
PTEIPIVASAKQALQVLLQPEGRKENHHEWISLLKNRKEKYPFSYKRNSESIKPQYAIDMLYEITKGEAIVTTDVGQHQM
WAAQYYPLKTPDKWVTSGGLGTMGFGFPAAIGAQIAKPDELVIAIVGDAGFQMTLQELSVLKEHSLPVKVFILNNEALGM
VRQWQDEFYNQRYSHSLLSCQPDFVALANAYGIKGVRIDDPLLAKKQLQHAIQLQEPVVIDCRVLQSEKVMPMVAPGKGV
HQMEGVEKR
>Mature_569_residues
MRTMSSKTEEKLATGAQLLLEALEKEGVEVIFGYPGGAVLPLYDALYDCEIPHILTRHEQGAIHAAEGYARITGNPGVVI
ATSGPGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDAFQEADIMGLTMPVTKHNYQVRKASDLPRIIKEAFHIARTG
RPGPVVIDLPKDMVVEQGERCSDVQMDLPGYNPNYEPNLLQINKLLQAIQVANKPLILAGAGVLHAKASKELTSFARKYE
IPVVHTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECDLLINIGARFDDRLTGNLAYFAKEATVAHIDIDPAEIGKNV
PTEIPIVASAKQALQVLLQPEGRKENHHEWISLLKNRKEKYPFSYKRNSESIKPQYAIDMLYEITKGEAIVTTDVGQHQM
WAAQYYPLKTPDKWVTSGGLGTMGFGFPAAIGAQIAKPDELVIAIVGDAGFQMTLQELSVLKEHSLPVKVFILNNEALGM
VRQWQDEFYNQRYSHSLLSCQPDFVALANAYGIKGVRIDDPLLAKKQLQHAIQLQEPVVIDCRVLQSEKVMPMVAPGKGV
HQMEGVEKR

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=556, Percent_Identity=24.2805755395683, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI21361361, Length=513, Percent_Identity=24.1715399610136, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=45.9363957597173, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI1790104, Length=566, Percent_Identity=40.9893992932862, Blast_Score=434, Evalue=1e-122,
Organism=Escherichia coli, GI1786717, Length=541, Percent_Identity=35.1201478743068, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1787096, Length=544, Percent_Identity=27.5735294117647, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1788716, Length=484, Percent_Identity=24.3801652892562, Blast_Score=139, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=485, Percent_Identity=29.0721649484536, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17531301, Length=485, Percent_Identity=29.0721649484536, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17542570, Length=558, Percent_Identity=24.1935483870968, Blast_Score=136, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=573, Percent_Identity=44.6771378708551, Blast_Score=479, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6320816, Length=487, Percent_Identity=24.2299794661191, Blast_Score=113, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6323163, Length=490, Percent_Identity=24.4897959183673, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19922626, Length=570, Percent_Identity=25.4385964912281, Blast_Score=155, Evalue=6e-38,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62327; Mature: 62327

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTMSSKTEEKLATGAQLLLEALEKEGVEVIFGYPGGAVLPLYDALYDCEIPHILTRHEQ
CCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHCCCC
GAIHAAEGYARITGNPGVVIATSGPGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDA
CCEEECCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHCCCH
FQEADIMGLTMPVTKHNYQVRKASDLPRIIKEAFHIARTGRPGPVVIDLPKDMVVEQGER
HCCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHCCCC
CSDVQMDLPGYNPNYEPNLLQINKLLQAIQVANKPLILAGAGVLHAKASKELTSFARKYE
CCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEHHHHHHHHHHHHHCC
IPVVHTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECDLLINIGARFDDRLTGNLAYF
CHHHHHHHHCCCCCCCCCEEEECCCCCCCEECCEEEEEEEEEEECCCCCCCCCCCCEEEE
AKEATVAHIDIDPAEIGKNVPTEIPIVASAKQALQVLLQPEGRKENHHEWISLLKNRKEK
EECCEEEEEECCHHHHCCCCCCCCCEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHC
YPFSYKRNSESIKPQYAIDMLYEITKGEAIVTTDVGQHQMWAAQYYPLKTPDKWVTSGGL
CCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCCCHHCCCCCC
GTMGFGFPAAIGAQIAKPDELVIAIVGDAGFQMTLQELSVLKEHSLPVKVFILNNEALGM
CCCCCCCHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHH
VRQWQDEFYNQRYSHSLLSCQPDFVALANAYGIKGVRIDDPLLAKKQLQHAIQLQEPVVI
HHHHHHHHHHHHHHCHHHCCCCCHHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCCCEEE
DCRVLQSEKVMPMVAPGKGVHQMEGVEKR
EEEEECCCCCCEEECCCCCCHHCCCCCCC
>Mature Secondary Structure
MRTMSSKTEEKLATGAQLLLEALEKEGVEVIFGYPGGAVLPLYDALYDCEIPHILTRHEQ
CCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHCCCC
GAIHAAEGYARITGNPGVVIATSGPGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDA
CCEEECCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHCCCH
FQEADIMGLTMPVTKHNYQVRKASDLPRIIKEAFHIARTGRPGPVVIDLPKDMVVEQGER
HCCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHCCCC
CSDVQMDLPGYNPNYEPNLLQINKLLQAIQVANKPLILAGAGVLHAKASKELTSFARKYE
CCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEHHHHHHHHHHHHHCC
IPVVHTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECDLLINIGARFDDRLTGNLAYF
CHHHHHHHHCCCCCCCCCEEEECCCCCCCEECCEEEEEEEEEEECCCCCCCCCCCCEEEE
AKEATVAHIDIDPAEIGKNVPTEIPIVASAKQALQVLLQPEGRKENHHEWISLLKNRKEK
EECCEEEEEECCHHHHCCCCCCCCCEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHC
YPFSYKRNSESIKPQYAIDMLYEITKGEAIVTTDVGQHQMWAAQYYPLKTPDKWVTSGGL
CCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCCCHHCCCCCC
GTMGFGFPAAIGAQIAKPDELVIAIVGDAGFQMTLQELSVLKEHSLPVKVFILNNEALGM
CCCCCCCHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHH
VRQWQDEFYNQRYSHSLLSCQPDFVALANAYGIKGVRIDDPLLAKKQLQHAIQLQEPVVI
HHHHHHHHHHHHHHCHHHCCCCCHHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCCCEEE
DCRVLQSEKVMPMVAPGKGVHQMEGVEKR
EEEEECCCCCCEEECCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]