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The map label for this gene is glcD [H]

Identifier: 218902503

GI number: 218902503

Start: 1321783

End: 1323195

Strand: Reverse

Name: glcD [H]

Synonym: BCAH820_1386

Alternate gene names: 218902503

Gene position: 1323195-1321783 (Counterclockwise)

Preceding gene: 218902504

Following gene: 218902502

Centisome position: 24.95

GC content: 39.63

Gene sequence:

>1413_bases
ATGTTAGAAAAACAAATTATTGATTCATTCGTATCCATTGTTGGCGAAGATAATGTAGATACATCCAATATGGGGCGTTT
AACGTATAGTTATGATGCCACTCCAAACTTCCAAGCAATGCCTGATGCAGTCATTGCTCCTCGTAATACAAATGAAATAG
CTGAAGTATTAAAAGTATGTAACACTCACAAAATCCCTGTATATGTTCGTGGTTCCGGGACGAACCTTTGCGCAGGAACA
TGTCCACTTGAAGGTGGTATCGTCCTTATCTTCCGCCATATGAATAACATTTTAGAAATTGATGAAGAAAATTTAACAAT
TACTGTACAAGCTGGTGTTATTACGCTTGATATTATTAAAGCGGTGGAAGAAAAAGGTTTATTTTATCCACCAGATCCAA
GCTCAATGAAAATTTCTACAATTGGCGGTAACATTAATGAAAACTCAGGTGGATTACGTGGATTAAAATATGGCGTAACA
CGTGATTATGTGATGGGGCTTGAACTTGTCTTACCAAATGGTGATATTATTCGTACTGGTGGTAAATTAGCAAAAGATGT
AGCTGGTTACGATTTAACTCGTTTATTTATCGGTTCTGAAGGGACTCTTGGCGTCGTAACAGAAGCAATATTAAAACTTG
TCCCTATGCCTGAAACGAAGAAAACGATGCTTGCACTATATGAAGATATTAACGAAGCTGCACGTGCTGTTTCTTCTATT
ATTGCAAATAAAATCATTCCAGCGACACTTGAGTTTTTAGACCAACCGACAATTGAAGTAGTAGAAGAGTTCGCACAAAT
CGGTTTACCAACTGATGTAAAAGCAATTTTATTAATTGAACAAGACGGTCCGCCTGAAGTTGTTAATCGAGATATTGAAA
AGATGGCTAACGTTTGTCGTTCCATGAATGCAGTTGATGTTCGCGTCGCAAAAGATGAAGCGGAAGCAGATGCGCTTCGA
ACAGCTCGCCGTAGTGCACTGTCAGCACTGGCAAGATTAAAGCCTACTACAATATTAGAAGATGCAACGGTACCACGCTC
ACAGATTGCTCCGATGGTTGAAGCTATTAATGCCATTGCCAAAAAATATAATATTCCTATTTGTACGTTTGGGCATGCTG
GCGATGGCAACCTACACCCAACTTGTATGACAGATGCTCGCAACGAAGAAGAAATGCACCGAGCTGAACAAGCTTTTGCT
GAAATATTTGCGAAAGCAATCGAACTTGGTGGCACAATCACTGGTGAACATGGTGTTGGTGCGATGAAAGCTCCGTATTT
AGAAATGAAGTTAGGTAAAGAAGGTATTACCGCTATGCAAGGAATTAAACACGCCTTCGATCCAAATAACATTATGAATC
CAGGAAAGATGTTCGCGAAGGACACTCGTAAAAGAGTGGTGGTTGAGCGATGA

Upstream 100 bases:

>100_bases
ATAGAAAGAATCCCCCGACTTTTTTATCATTATAATGAAAGCACTGTGACAACGTTTTTTATTATGACTCATTACGTTAA
CAGTTTGAGGGGGATTTCCA

Downstream 100 bases:

>100_bases
CAACATTAAATAAAGAAAACATTCAAAAAGAATTTAAAGAACGATTAAGTGAAGATGAACTACTAAACTGTATGCGATGC
GGTTTCTGTTTACCAACTTG

Product: glycolate oxidase, subunit GlcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MLEKQIIDSFVSIVGEDNVDTSNMGRLTYSYDATPNFQAMPDAVIAPRNTNEIAEVLKVCNTHKIPVYVRGSGTNLCAGT
CPLEGGIVLIFRHMNNILEIDEENLTITVQAGVITLDIIKAVEEKGLFYPPDPSSMKISTIGGNINENSGGLRGLKYGVT
RDYVMGLELVLPNGDIIRTGGKLAKDVAGYDLTRLFIGSEGTLGVVTEAILKLVPMPETKKTMLALYEDINEAARAVSSI
IANKIIPATLEFLDQPTIEVVEEFAQIGLPTDVKAILLIEQDGPPEVVNRDIEKMANVCRSMNAVDVRVAKDEAEADALR
TARRSALSALARLKPTTILEDATVPRSQIAPMVEAINAIAKKYNIPICTFGHAGDGNLHPTCMTDARNEEEMHRAEQAFA
EIFAKAIELGGTITGEHGVGAMKAPYLEMKLGKEGITAMQGIKHAFDPNNIMNPGKMFAKDTRKRVVVER

Sequences:

>Translated_470_residues
MLEKQIIDSFVSIVGEDNVDTSNMGRLTYSYDATPNFQAMPDAVIAPRNTNEIAEVLKVCNTHKIPVYVRGSGTNLCAGT
CPLEGGIVLIFRHMNNILEIDEENLTITVQAGVITLDIIKAVEEKGLFYPPDPSSMKISTIGGNINENSGGLRGLKYGVT
RDYVMGLELVLPNGDIIRTGGKLAKDVAGYDLTRLFIGSEGTLGVVTEAILKLVPMPETKKTMLALYEDINEAARAVSSI
IANKIIPATLEFLDQPTIEVVEEFAQIGLPTDVKAILLIEQDGPPEVVNRDIEKMANVCRSMNAVDVRVAKDEAEADALR
TARRSALSALARLKPTTILEDATVPRSQIAPMVEAINAIAKKYNIPICTFGHAGDGNLHPTCMTDARNEEEMHRAEQAFA
EIFAKAIELGGTITGEHGVGAMKAPYLEMKLGKEGITAMQGIKHAFDPNNIMNPGKMFAKDTRKRVVVER
>Mature_470_residues
MLEKQIIDSFVSIVGEDNVDTSNMGRLTYSYDATPNFQAMPDAVIAPRNTNEIAEVLKVCNTHKIPVYVRGSGTNLCAGT
CPLEGGIVLIFRHMNNILEIDEENLTITVQAGVITLDIIKAVEEKGLFYPPDPSSMKISTIGGNINENSGGLRGLKYGVT
RDYVMGLELVLPNGDIIRTGGKLAKDVAGYDLTRLFIGSEGTLGVVTEAILKLVPMPETKKTMLALYEDINEAARAVSSI
IANKIIPATLEFLDQPTIEVVEEFAQIGLPTDVKAILLIEQDGPPEVVNRDIEKMANVCRSMNAVDVRVAKDEAEADALR
TARRSALSALARLKPTTILEDATVPRSQIAPMVEAINAIAKKYNIPICTFGHAGDGNLHPTCMTDARNEEEMHRAEQAFA
EIFAKAIELGGTITGEHGVGAMKAPYLEMKLGKEGITAMQGIKHAFDPNNIMNPGKMFAKDTRKRVVVER

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=467, Percent_Identity=30.6209850107066, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI37595754, Length=490, Percent_Identity=29.1836734693878, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI119964728, Length=432, Percent_Identity=28.0092592592593, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4501993, Length=228, Percent_Identity=28.5087719298246, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1789351, Length=430, Percent_Identity=38.1395348837209, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1787977, Length=512, Percent_Identity=27.1484375, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI48994907, Length=484, Percent_Identity=25.4132231404959, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI48994914, Length=177, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71992373, Length=467, Percent_Identity=32.5481798715203, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI71992381, Length=467, Percent_Identity=32.1199143468951, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI71992392, Length=384, Percent_Identity=32.5520833333333, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17534361, Length=465, Percent_Identity=28.8172043010753, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17556096, Length=219, Percent_Identity=29.2237442922374, Blast_Score=113, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6320027, Length=445, Percent_Identity=30.7865168539326, Blast_Score=206, Evalue=9e-54,
Organism=Saccharomyces cerevisiae, GI6320764, Length=474, Percent_Identity=28.4810126582279, Blast_Score=183, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6320023, Length=453, Percent_Identity=28.2560706401766, Blast_Score=173, Evalue=6e-44,
Organism=Drosophila melanogaster, GI18921117, Length=425, Percent_Identity=29.1764705882353, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24639277, Length=425, Percent_Identity=29.1764705882353, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24639275, Length=425, Percent_Identity=29.1764705882353, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24653753, Length=255, Percent_Identity=27.4509803921569, Blast_Score=116, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 51020; Mature: 51020

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEKQIIDSFVSIVGEDNVDTSNMGRLTYSYDATPNFQAMPDAVIAPRNTNEIAEVLKVC
CCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHH
NTHKIPVYVRGSGTNLCAGTCPLEGGIVLIFRHMNNILEIDEENLTITVQAGVITLDIIK
CCCCCEEEEECCCCCEEEECCCCCCCEEEEEEHHHHHEEECCCCEEEEEECCEEHHHHHH
AVEEKGLFYPPDPSSMKISTIGGNINENSGGLRGLKYGVTRDYVMGLELVLPNGDIIRTG
HHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEECCCCHHHHEEEEEEECCCCEEECC
GKLAKDVAGYDLTRLFIGSEGTLGVVTEAILKLVPMPETKKTMLALYEDINEAARAVSSI
CHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
IANKIIPATLEFLDQPTIEVVEEFAQIGLPTDVKAILLIEQDGPPEVVNRDIEKMANVCR
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
SMNAVDVRVAKDEAEADALRTARRSALSALARLKPTTILEDATVPRSQIAPMVEAINAIA
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHH
KKYNIPICTFGHAGDGNLHPTCMTDARNEEEMHRAEQAFAEIFAKAIELGGTITGEHGVG
HHCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCC
AMKAPYLEMKLGKEGITAMQGIKHAFDPNNIMNPGKMFAKDTRKRVVVER
CCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLEKQIIDSFVSIVGEDNVDTSNMGRLTYSYDATPNFQAMPDAVIAPRNTNEIAEVLKVC
CCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHH
NTHKIPVYVRGSGTNLCAGTCPLEGGIVLIFRHMNNILEIDEENLTITVQAGVITLDIIK
CCCCCEEEEECCCCCEEEECCCCCCCEEEEEEHHHHHEEECCCCEEEEEECCEEHHHHHH
AVEEKGLFYPPDPSSMKISTIGGNINENSGGLRGLKYGVTRDYVMGLELVLPNGDIIRTG
HHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEECCCCHHHHEEEEEEECCCCEEECC
GKLAKDVAGYDLTRLFIGSEGTLGVVTEAILKLVPMPETKKTMLALYEDINEAARAVSSI
CHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
IANKIIPATLEFLDQPTIEVVEEFAQIGLPTDVKAILLIEQDGPPEVVNRDIEKMANVCR
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
SMNAVDVRVAKDEAEADALRTARRSALSALARLKPTTILEDATVPRSQIAPMVEAINAIA
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHH
KKYNIPICTFGHAGDGNLHPTCMTDARNEEEMHRAEQAFAEIFAKAIELGGTITGEHGVG
HHCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCC
AMKAPYLEMKLGKEGITAMQGIKHAFDPNNIMNPGKMFAKDTRKRVVVER
CCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]