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The map label for this gene is lplA [H]

Identifier: 218902285

GI number: 218902285

Start: 1114574

End: 1115563

Strand: Direct

Name: lplA [H]

Synonym: BCAH820_1167

Alternate gene names: 218902285

Gene position: 1114574-1115563 (Clockwise)

Preceding gene: 218902284

Following gene: 218902286

Centisome position: 21.02

GC content: 36.46

Gene sequence:

>990_bases
ATGTTATTTATTGATAATAAAGGGATTACAGATCCTAGAATAAACTTAGCGATTGAAGAATATTGTGTGAAGAATTTAGA
TATTAACGAAACATATTTACTGTTCTACATTAATGAGCCTTCGATTATTATTGGTAAAAACCAAAACACAGTAGAAGAAA
TTAATGCAGATTACGTAAAAGAAAAAGGTATTCATGTCGTTCGTCGTCTATCAGGCGGAGGCGCAGTATATCATGATTTA
GGAAACTTAAACTTCAGCTTTATTACGAAAGATGATGGGGACAGTTTCAGTAACTTCAAAAAATTCACCGAGCCTGTAAC
GAAAGCGCTTGGTAAATTAGGCGTAAATGCAGAACTAAGTGGTCGTAATGACATTTTAGCTGAAGGCAGAAAAATTTCAG
GTAACGCGCAGTTCTCAACGAAAGGTCGTATGTTCAGTCACGGTACGTTACTATTTGACTCTGAAATCGATCACGTCGTA
TCAGCGTTAAAAGTAAAAATGGATAAAATTCAATCAAAAGGAATTAAATCAATTCGTAGCCGCGTTGCGAACATTACAGA
GTTCTTAAACGAAGAAATGACGACAGAAGAGTTTAGACAACTTCTTCTAGAAACAATTTTTGAAGGAGAGACAGAAATCC
CAACGTATGAATTAACAGAAGAGGATTGGAAAGAAATCCACAAACTTTCTGAAGAGCGCTACCGCAATTGGGACTGGAAC
TACGGAAAATCTCCGAAGTTCAACTTACAACATTCACACCGCTTCCCAGTTGGACAAGTTGACGTTCGTCTTGAAGTGAA
AAAAGGAACAGTAACAGAATGCAAAATTTACGGTGACTTCTTCGGTTCACTAGATGTGCATGATATTGAAGAACGTCTAA
CAGGCGTACAATTTGATAAAGATGCATTCACTACAGCTTTAGAAGGTGTAGATATCGCACGTTACTTCGGTAATATTACA
ACAGAAGATTTCTTACATTTGTTCTTCTAA

Upstream 100 bases:

>100_bases
AGGCAATCCAGCTGATTTAGTAACAGAAGCAAAACAAATTTTCAGTGGCCATATTACGTTAGCGCATAGTGGCTACGTAT
GGAACTCATGAGGTGATACG

Downstream 100 bases:

>100_bases
AAATAAGGGAGGCTACCGTAACTGGCAGCCTCTTTCTTTTTGCGTAAATTGTCGCGTTTTGTCGGTAAGTTGATATATTT
AAAAAATCGCTGATATATTC

Product: putative lipoate-protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MLFIDNKGITDPRINLAIEEYCVKNLDINETYLLFYINEPSIIIGKNQNTVEEINADYVKEKGIHVVRRLSGGGAVYHDL
GNLNFSFITKDDGDSFSNFKKFTEPVTKALGKLGVNAELSGRNDILAEGRKISGNAQFSTKGRMFSHGTLLFDSEIDHVV
SALKVKMDKIQSKGIKSIRSRVANITEFLNEEMTTEEFRQLLLETIFEGETEIPTYELTEEDWKEIHKLSEERYRNWDWN
YGKSPKFNLQHSHRFPVGQVDVRLEVKKGTVTECKIYGDFFGSLDVHDIEERLTGVQFDKDAFTTALEGVDIARYFGNIT
TEDFLHLFF

Sequences:

>Translated_329_residues
MLFIDNKGITDPRINLAIEEYCVKNLDINETYLLFYINEPSIIIGKNQNTVEEINADYVKEKGIHVVRRLSGGGAVYHDL
GNLNFSFITKDDGDSFSNFKKFTEPVTKALGKLGVNAELSGRNDILAEGRKISGNAQFSTKGRMFSHGTLLFDSEIDHVV
SALKVKMDKIQSKGIKSIRSRVANITEFLNEEMTTEEFRQLLLETIFEGETEIPTYELTEEDWKEIHKLSEERYRNWDWN
YGKSPKFNLQHSHRFPVGQVDVRLEVKKGTVTECKIYGDFFGSLDVHDIEERLTGVQFDKDAFTTALEGVDIARYFGNIT
TEDFLHLFF
>Mature_329_residues
MLFIDNKGITDPRINLAIEEYCVKNLDINETYLLFYINEPSIIIGKNQNTVEEINADYVKEKGIHVVRRLSGGGAVYHDL
GNLNFSFITKDDGDSFSNFKKFTEPVTKALGKLGVNAELSGRNDILAEGRKISGNAQFSTKGRMFSHGTLLFDSEIDHVV
SALKVKMDKIQSKGIKSIRSRVANITEFLNEEMTTEEFRQLLLETIFEGETEIPTYELTEEDWKEIHKLSEERYRNWDWN
YGKSPKFNLQHSHRFPVGQVDVRLEVKKGTVTECKIYGDFFGSLDVHDIEERLTGVQFDKDAFTTALEGVDIARYFGNIT
TEDFLHLFF

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=303, Percent_Identity=30.6930693069307, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI21729884, Length=303, Percent_Identity=30.6930693069307, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI21729882, Length=303, Percent_Identity=30.6930693069307, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI21729880, Length=303, Percent_Identity=30.6930693069307, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI21729878, Length=303, Percent_Identity=30.6930693069307, Blast_Score=124, Evalue=9e-29,
Organism=Escherichia coli, GI1790846, Length=307, Percent_Identity=33.8762214983713, Blast_Score=175, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI71981909, Length=244, Percent_Identity=35.655737704918, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6322415, Length=283, Percent_Identity=30.3886925795053, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI28573464, Length=283, Percent_Identity=31.8021201413428, Blast_Score=117, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 37696; Mature: 37696

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFIDNKGITDPRINLAIEEYCVKNLDINETYLLFYINEPSIIIGKNQNTVEEINADYVK
CEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCHHHHHCHHHHH
EKGIHVVRRLSGGGAVYHDLGNLNFSFITKDDGDSFSNFKKFTEPVTKALGKLGVNAELS
HCCHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEC
GRNDILAEGRKISGNAQFSTKGRMFSHGTLLFDSEIDHVVSALKVKMDKIQSKGIKSIRS
CCCCHHHCCCCCCCCCEECCCCCEEECCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
RVANITEFLNEEMTTEEFRQLLLETIFEGETEIPTYELTEEDWKEIHKLSEERYRNWDWN
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCC
YGKSPKFNLQHSHRFPVGQVDVRLEVKKGTVTECKIYGDFFGSLDVHDIEERLTGVQFDK
CCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCH
DAFTTALEGVDIARYFGNITTEDFLHLFF
HHHHHHHCCHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MLFIDNKGITDPRINLAIEEYCVKNLDINETYLLFYINEPSIIIGKNQNTVEEINADYVK
CEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCHHHHHCHHHHH
EKGIHVVRRLSGGGAVYHDLGNLNFSFITKDDGDSFSNFKKFTEPVTKALGKLGVNAELS
HCCHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEC
GRNDILAEGRKISGNAQFSTKGRMFSHGTLLFDSEIDHVVSALKVKMDKIQSKGIKSIRS
CCCCHHHCCCCCCCCCEECCCCCEEECCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
RVANITEFLNEEMTTEEFRQLLLETIFEGETEIPTYELTEEDWKEIHKLSEERYRNWDWN
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCC
YGKSPKFNLQHSHRFPVGQVDVRLEVKKGTVTECKIYGDFFGSLDVHDIEERLTGVQFDK
CCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCH
DAFTTALEGVDIARYFGNITTEDFLHLFF
HHHHHHHCCHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]