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The map label for this gene is pbuO [H]

Identifier: 218901888

GI number: 218901888

Start: 728471

End: 729763

Strand: Direct

Name: pbuO [H]

Synonym: BCAH820_0749

Alternate gene names: 218901888

Gene position: 728471-729763 (Clockwise)

Preceding gene: 218901885

Following gene: 218901889

Centisome position: 13.74

GC content: 35.58

Gene sequence:

>1293_bases
ATGTTTCAATTACAAAAATATAACACTTCTGTGAAGCAAGAGTTTTTGGCTGGCATCACAACTTTTTTTACATTTGCGTA
TATTCTTGTCATCAATCCGAAAATATTATCTGATGCAGGTGTACCATTTGACCAAGCGTTTACAGCAACAATTATTGCAA
CTGTTGTTGGAACAATAGGTATGGCGATTTTTGCTAATTATCCAATTGTTATTGCTCCAGCTATGGGGATGAACGCATAC
TTTGCGTATTCTGTTGTACAGAAAGCAGAAGGGATTACATATGTAATTGCCTTTTCAGCGGTATTTGTAACAGGGATTAT
TTTTCTTTTATTATCTTTTACTTCGTTTAGGCAAAAGTTAATTTTAGCAATTCCTGATAGTTTAAAGCATGCAATTGCAG
GTGGTATTGGACTATTTATTGCTTTTATTGGATTACGCCTTTCTGGAATTATCGTTGATCATCCATCAAATTTAGTTACG
ATTGGTGATTTTCATTCTCCAGCCGTTATTTTAACTCTTATTGGTTTAATATTAGCGGCGGTATTAATGGCATTGCGTGT
GAGCGGTGCATTATTTATTAGTATGATTGTGACAGGAGTTATCGCCTTTTTTACAGGGCAACTGAAGTTCACGGATAAAA
TTGTTGCGATGCCTCATTTACCAGAGGGTATTATCGTTTCAAACCCAATCAATGCCTTTTCAGATGTAATTGAGTACGGC
TTATATGGTGTTGTATTTTCATTTTTACTTGTACTGTTATTTGATACGACAGGTGCATTACTTGGATTAATTAAACAAGC
GGGTTTAATTACGGATAATACAGAGAAACGTTTTGGTAAAGCGTTTATTGCGGATGCAATTGGCGGTACTACAGGCTCTA
TCTTTGGGACAAGTCCAACAGCAGCGACGATTGAATCGTCAGCTGGTATTGCTGCAGGTGGTAAAACGGGATTAACAGGT
ATTGTAGTAGTCGGCCTAACGATTATAACGGCGTTTTTCAGCCCTGTTATTGCTTCTTTATCAAGTGTAGCTGCTATTAC
AGCTCCTTCATTAATTATTGTAGGTAGTTTAATGGCACAAAGTATTCGTGATATTAATTGGAATGAATTTGAAGATGCAT
TACCAGCATTTTTAATTTTCGTTGGTATTCCGTTAACGTCTAGTATTGCGAATGGAATTGCAATTGGATTTTTAGTGTAT
CCAGTCTTGAAAATTGTGAAAGGGAAAGGGATGGAAGTACATCCATTACTATACTTATTTACAATTTTATTTGGATGTCA
TTTATTCTTATAA

Upstream 100 bases:

>100_bases
TTCTTTTTATTGATTGTTAAGATTAAGTATTATATTTTCTCGCATACTAGTAAGAAGAGAATGTGTGATAATAATTCAGG
TGTAGGAAGGGTACATAAGC

Downstream 100 bases:

>100_bases
TATAGGAGAGGGGGCTTCTTATGAGGGGCTCCTTTTTTGTGTATAATAAAGTGAAACTTAAATTAGTAATGTGAAATATA
AATTACTTATTGGAATATAT

Product: xanthine/uracil permease family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MFQLQKYNTSVKQEFLAGITTFFTFAYILVINPKILSDAGVPFDQAFTATIIATVVGTIGMAIFANYPIVIAPAMGMNAY
FAYSVVQKAEGITYVIAFSAVFVTGIIFLLLSFTSFRQKLILAIPDSLKHAIAGGIGLFIAFIGLRLSGIIVDHPSNLVT
IGDFHSPAVILTLIGLILAAVLMALRVSGALFISMIVTGVIAFFTGQLKFTDKIVAMPHLPEGIIVSNPINAFSDVIEYG
LYGVVFSFLLVLLFDTTGALLGLIKQAGLITDNTEKRFGKAFIADAIGGTTGSIFGTSPTAATIESSAGIAAGGKTGLTG
IVVVGLTIITAFFSPVIASLSSVAAITAPSLIIVGSLMAQSIRDINWNEFEDALPAFLIFVGIPLTSSIANGIAIGFLVY
PVLKIVKGKGMEVHPLLYLFTILFGCHLFL

Sequences:

>Translated_430_residues
MFQLQKYNTSVKQEFLAGITTFFTFAYILVINPKILSDAGVPFDQAFTATIIATVVGTIGMAIFANYPIVIAPAMGMNAY
FAYSVVQKAEGITYVIAFSAVFVTGIIFLLLSFTSFRQKLILAIPDSLKHAIAGGIGLFIAFIGLRLSGIIVDHPSNLVT
IGDFHSPAVILTLIGLILAAVLMALRVSGALFISMIVTGVIAFFTGQLKFTDKIVAMPHLPEGIIVSNPINAFSDVIEYG
LYGVVFSFLLVLLFDTTGALLGLIKQAGLITDNTEKRFGKAFIADAIGGTTGSIFGTSPTAATIESSAGIAAGGKTGLTG
IVVVGLTIITAFFSPVIASLSSVAAITAPSLIIVGSLMAQSIRDINWNEFEDALPAFLIFVGIPLTSSIANGIAIGFLVY
PVLKIVKGKGMEVHPLLYLFTILFGCHLFL
>Mature_430_residues
MFQLQKYNTSVKQEFLAGITTFFTFAYILVINPKILSDAGVPFDQAFTATIIATVVGTIGMAIFANYPIVIAPAMGMNAY
FAYSVVQKAEGITYVIAFSAVFVTGIIFLLLSFTSFRQKLILAIPDSLKHAIAGGIGLFIAFIGLRLSGIIVDHPSNLVT
IGDFHSPAVILTLIGLILAAVLMALRVSGALFISMIVTGVIAFFTGQLKFTDKIVAMPHLPEGIIVSNPINAFSDVIEYG
LYGVVFSFLLVLLFDTTGALLGLIKQAGLITDNTEKRFGKAFIADAIGGTTGSIFGTSPTAATIESSAGIAAGGKTGLTG
IVVVGLTIITAFFSPVIASLSSVAAITAPSLIIVGSLMAQSIRDINWNEFEDALPAFLIFVGIPLTSSIANGIAIGFLVY
PVLKIVKGKGMEVHPLLYLFTILFGCHLFL

Specific function: Involved in the uptake of the purine bases hypoxanthine and guanine. May work at purine concentrations higher than 100 uM [H]

COG id: COG2252

COG function: function code R; Permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. AzgA purine transporter (TC 2.A.1.40) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082309, Length=431, Percent_Identity=37.5870069605568, Blast_Score=274, Evalue=7e-75,
Organism=Escherichia coli, GI1790150, Length=424, Percent_Identity=38.6792452830189, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI1790499, Length=425, Percent_Identity=31.2941176470588, Blast_Score=181, Evalue=9e-47,
Organism=Escherichia coli, GI48994909, Length=412, Percent_Identity=32.5242718446602, Blast_Score=174, Evalue=7e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 45400; Mature: 45400

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQLQKYNTSVKQEFLAGITTFFTFAYILVINPKILSDAGVPFDQAFTATIIATVVGTIG
CCCCHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHH
MAIFANYPIVIAPAMGMNAYFAYSVVQKAEGITYVIAFSAVFVTGIIFLLLSFTSFRQKL
HHHHHCCCEEEECCCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHE
ILAIPDSLKHAIAGGIGLFIAFIGLRLSGIIVDHPSNLVTIGDFHSPAVILTLIGLILAA
EHCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEECCCCCHHHHHHHHHHHHHH
VLMALRVSGALFISMIVTGVIAFFTGQLKFTDKIVAMPHLPEGIIVSNPINAFSDVIEYG
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCEEECCCCCCCCEECCCHHHHHHHHHHH
LYGVVFSFLLVLLFDTTGALLGLIKQAGLITDNTEKRFGKAFIADAIGGTTGSIFGTSPT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
AATIESSAGIAAGGKTGLTGIVVVGLTIITAFFSPVIASLSSVAAITAPSLIIVGSLMAQ
CHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
SIRDINWNEFEDALPAFLIFVGIPLTSSIANGIAIGFLVYPVLKIVKGKGMEVHPLLYLF
HHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
TILFGCHLFL
HHHHHHHHCC
>Mature Secondary Structure
MFQLQKYNTSVKQEFLAGITTFFTFAYILVINPKILSDAGVPFDQAFTATIIATVVGTIG
CCCCHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHH
MAIFANYPIVIAPAMGMNAYFAYSVVQKAEGITYVIAFSAVFVTGIIFLLLSFTSFRQKL
HHHHHCCCEEEECCCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHE
ILAIPDSLKHAIAGGIGLFIAFIGLRLSGIIVDHPSNLVTIGDFHSPAVILTLIGLILAA
EHCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEECCCCCHHHHHHHHHHHHHH
VLMALRVSGALFISMIVTGVIAFFTGQLKFTDKIVAMPHLPEGIIVSNPINAFSDVIEYG
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCEEECCCCCCCCEECCCHHHHHHHHHHH
LYGVVFSFLLVLLFDTTGALLGLIKQAGLITDNTEKRFGKAFIADAIGGTTGSIFGTSPT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
AATIESSAGIAAGGKTGLTGIVVVGLTIITAFFSPVIASLSSVAAITAPSLIIVGSLMAQ
CHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
SIRDINWNEFEDALPAFLIFVGIPLTSSIANGIAIGFLVYPVLKIVKGKGMEVHPLLYLF
HHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
TILFGCHLFL
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 11591660 [H]