Click here to switch to the map view.

The map label for this gene is malK [H]

Identifier: 218901771

GI number: 218901771

Start: 598401

End: 600005

Strand: Direct

Name: malK [H]

Synonym: BCAH820_0632

Alternate gene names: 218901771

Gene position: 598401-600005 (Clockwise)

Preceding gene: 218901770

Following gene: 218901772

Centisome position: 11.28

GC content: 33.89

Gene sequence:

>1605_bases
ATGAAAAAAAGGAAAAGACTATGGAATTTATGGAAAACGATTACATTGTTAGTTTGTACAGTTGTAATTTTTTCTTTACT
TGTGACAGATATATTAATTAGTCACAATGTAGAACGGACAACGGAGGATAGCCAAGCAGAGAAAGCAAAAACAATTGCAC
ACATTGTGGCGAATGATTCAATTGTAATCGATGGTTTAATTGGGAAAGCAGATACTTCTGCAATTCAAACGTATACAAAT
AGAATATTAAAAAACACAGGTGTTCAATTTATTGTAGTTATGGACATGAATGGAATAAGAAAGTCACATCCAAATCCTCA
AAAAATAGGTTATCATTTTATTGGGGGAGATGAAGGACCTGCATTGAAAGGAAAGGAACATGTATCGCTGGCAGAAGGAA
CTTTAGGTATTTCAATGCGAGTGTTTGTACCTGTATTTTCTGAAACAGGTGAACAACTTGGAGCGGTGGCTGTTGGTATT
TCAGCAGATAATGTAAAAGAGAGAGTTAAGGAAAGTAGACATATCATTTATATTGGAGTTGGAGTTGGGGTACTAGTCGG
AATGATAGGAGCAATATTGCTAGCTAGACATATAAAGAAAAGTTTATTCGGCCTTGAACCTCATAGGATAGCGAAAATTC
TTGAAGAAAGAAATACGATGCTACAATCTGTAAAGGAAGGTATTATTGCCGTAGATAAAGAGGCCAATGTAACTTTAATA
AATAATGAGGCAAAACGATTGTTTAAGAAAAGTGGATTGGAAGAAGATTTTATCGGTAAAGATGTTGAGTTGTATATGCC
TAATTCACGTATAAAAGAAGTATTACAAACGGGAGAGGTACAATTAAACGAAGAACAAAATATTTATGGAATTACGATTG
TGACGAATCGTGTTCCTTTATATGTAAAAGGAGAAATAGTTGGCGCGATTGCAACATTCCGTGATAAAACAGAGATTAGA
AAATTAGCAGAAGAATTAACTGGTATTAGGCTATATGCGGAAGCATTACGAGCACAATCGCATGAATTTATGAATAAAAT
GCATGTCGTATTAGGGCTTACACACATGAAACAGTATGAAGAATTACAGAAGTATATAAGCGGTATGGTATCAGAGCACC
AATATGAAATTGGTGGGGTTATGAAAAGAATAAAAAGTCCAGTGTTTGCTGGTTTTTTACTCGGAAAACTGAGCTATGCT
AGAGAGAAAAATATAAAATTAATTATAAGTGAAGATTCTTACATGCCAGAAATAGATGATGAAAGTATTACTCATGAACT
AATTACGATTGTCGGAAATTTAATTGATAATGCATTAGAGGCAGTGACGAATTGTGAAAAGAAGCAAGTTGAAGTTGAGA
TACAATATGGGAATACATTAATTATTACAGTGCAAGATACGGGAAAAGGTATACAAGAAAAAGAAATAGAGGCATTATTT
ACGAAAGGTTATTCTACAAAGGGAGATAACCGCGGTTATGGTTTGTATCTTGTAAAAGAAAGTATACAGCGAATAAATGG
GGAAATTCATATGCATTCATTAGTAGGAAAGGGAACAAAGATAACAATTGAAATACCTAAAGGTAGGGATGAGAGGCAAA
TATGA

Upstream 100 bases:

>100_bases
GACTAGTAAATTTAAAGTATAATAACAGCAGATAAGTAAGGTGAGAATAATATCACCTTACTTATTTTTCTATTCAGCAA
TTAGGGGAGTCATTAACGGG

Downstream 100 bases:

>100_bases
TTAAAGTTTTAATTGTAGAAGATGACCCGATGGTAGCAATGTTAAATACGCATTATTTAGAGCAAGTAGGAGGGTTTGAA
CTTGTTCAAGCAGTTAATTC

Product: sensory histidine kinase DcuS

Products: NA

Alternate protein names: Malate kinase sensor [H]

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MKKRKRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERTTEDSQAEKAKTIAHIVANDSIVIDGLIGKADTSAIQTYTN
RILKNTGVQFIVVMDMNGIRKSHPNPQKIGYHFIGGDEGPALKGKEHVSLAEGTLGISMRVFVPVFSETGEQLGAVAVGI
SADNVKERVKESRHIIYIGVGVGVLVGMIGAILLARHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEANVTLI
NNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPLYVKGEIVGAIATFRDKTEIR
KLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYEELQKYISGMVSEHQYEIGGVMKRIKSPVFAGFLLGKLSYA
REKNIKLIISEDSYMPEIDDESITHELITIVGNLIDNALEAVTNCEKKQVEVEIQYGNTLIITVQDTGKGIQEKEIEALF
TKGYSTKGDNRGYGLYLVKESIQRINGEIHMHSLVGKGTKITIEIPKGRDERQI

Sequences:

>Translated_534_residues
MKKRKRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERTTEDSQAEKAKTIAHIVANDSIVIDGLIGKADTSAIQTYTN
RILKNTGVQFIVVMDMNGIRKSHPNPQKIGYHFIGGDEGPALKGKEHVSLAEGTLGISMRVFVPVFSETGEQLGAVAVGI
SADNVKERVKESRHIIYIGVGVGVLVGMIGAILLARHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEANVTLI
NNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPLYVKGEIVGAIATFRDKTEIR
KLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYEELQKYISGMVSEHQYEIGGVMKRIKSPVFAGFLLGKLSYA
REKNIKLIISEDSYMPEIDDESITHELITIVGNLIDNALEAVTNCEKKQVEVEIQYGNTLIITVQDTGKGIQEKEIEALF
TKGYSTKGDNRGYGLYLVKESIQRINGEIHMHSLVGKGTKITIEIPKGRDERQI
>Mature_534_residues
MKKRKRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERTTEDSQAEKAKTIAHIVANDSIVIDGLIGKADTSAIQTYTN
RILKNTGVQFIVVMDMNGIRKSHPNPQKIGYHFIGGDEGPALKGKEHVSLAEGTLGISMRVFVPVFSETGEQLGAVAVGI
SADNVKERVKESRHIIYIGVGVGVLVGMIGAILLARHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEANVTLI
NNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPLYVKGEIVGAIATFRDKTEIR
KLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYEELQKYISGMVSEHQYEIGGVMKRIKSPVFAGFLLGKLSYA
REKNIKLIISEDSYMPEIDDESITHELITIVGNLIDNALEAVTNCEKKQVEVEIQYGNTLIITVQDTGKGIQEKEIEALF
TKGYSTKGDNRGYGLYLVKESIQRINGEIHMHSLVGKGTKITIEIPKGRDERQI

Specific function: Member of a two-component regulatory system malK/malR. Involved in the activation of maeA, maeN and yflS in presence of malate. Probably activates malR by phosphorylation [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790567, Length=526, Percent_Identity=40.3041825095057, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1786837, Length=527, Percent_Identity=30.3605313092979, Blast_Score=244, Evalue=9e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59660; Mature: 59660

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRKRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERTTEDSQAEKAKTIAHIVANDS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
IVIDGLIGKADTSAIQTYTNRILKNTGVQFIVVMDMNGIRKSHPNPQKIGYHFIGGDEGP
EEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHCCEEEECCCCCC
ALKGKEHVSLAEGTLGISMRVFVPVFSETGEQLGAVAVGISADNVKERVKESRHIIYIGV
CCCCCCCCEEECCCCCEEEEEEEEEHHHCCHHHCEEEEECCHHHHHHHHHHCCCEEEEEC
GVGVLVGMIGAILLARHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEANVTLI
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEE
NNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPL
CHHHHHHHHHCCCCHHHCCCCEEEECCCHHHHHHHHCCCEEECCCCCEEEEEEEECCCCE
YVKGEIVGAIATFRDKTEIRKLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYE
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELQKYISGMVSEHQYEIGGVMKRIKSPVFAGFLLGKLSYAREKNIKLIISEDSYMPEIDD
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
ESITHELITIVGNLIDNALEAVTNCEKKQVEVEIQYGNTLIITVQDTGKGIQEKEIEALF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEEEECCCCCCHHHHHHHHH
TKGYSTKGDNRGYGLYLVKESIQRINGEIHMHSLVGKGTKITIEIPKGRDERQI
HCCCCCCCCCCCEEEEEEHHHHHHHCCCEEEEHHHCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MKKRKRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERTTEDSQAEKAKTIAHIVANDS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
IVIDGLIGKADTSAIQTYTNRILKNTGVQFIVVMDMNGIRKSHPNPQKIGYHFIGGDEGP
EEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHCCEEEECCCCCC
ALKGKEHVSLAEGTLGISMRVFVPVFSETGEQLGAVAVGISADNVKERVKESRHIIYIGV
CCCCCCCCEEECCCCCEEEEEEEEEHHHCCHHHCEEEEECCHHHHHHHHHHCCCEEEEEC
GVGVLVGMIGAILLARHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEANVTLI
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEE
NNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPL
CHHHHHHHHHCCCCHHHCCCCEEEECCCHHHHHHHHCCCEEECCCCCEEEEEEEECCCCE
YVKGEIVGAIATFRDKTEIRKLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYE
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELQKYISGMVSEHQYEIGGVMKRIKSPVFAGFLLGKLSYAREKNIKLIISEDSYMPEIDD
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
ESITHELITIVGNLIDNALEAVTNCEKKQVEVEIQYGNTLIITVQDTGKGIQEKEIEALF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEEEECCCCCCHHHHHHHHH
TKGYSTKGDNRGYGLYLVKESIQRINGEIHMHSLVGKGTKITIEIPKGRDERQI
HCCCCCCCCCCCEEEEEEHHHHHHHCCCEEEEHHHCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 12949159; 12949160 [H]