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The map label for this gene is citS [H]

Identifier: 218901754

GI number: 218901754

Start: 579790

End: 581394

Strand: Direct

Name: citS [H]

Synonym: BCAH820_0615

Alternate gene names: 218901754

Gene position: 579790-581394 (Clockwise)

Preceding gene: 218901753

Following gene: 218901755

Centisome position: 10.93

GC content: 33.71

Gene sequence:

>1605_bases
TTGAAATTTAAAAAATTAAAACTACAACCGAGAATTACATTAACTATTAGTGCACTTATTTTAGTTGTACTCCTTTTAAC
GAGTTATTTATTTTATTACATATTATCTGAAACAGTAGAAGAGCAAATTGGCAAAAGAGCTTTGCATGTTGCGAAAACGG
TTGCTGCTATACCAGAAATCAAGGAAGCTTTTCAAACAGAAAATCCAGCTTCTATTATTCAACCGATTGCGGAAAAAATT
CGAATGGATACAGATGCTGATTTTATTGTAGTTGGAAATAAAGAAGGGATTCGTTATGCACATCCTAGGAAAGATAAAAT
AGGAGAAGCAATGGTTGGCGGAGATAACAAAGGAGTTTTATTAGAAGGGAAATCTTACGTTTCGAAAGCAACTGGATCAT
TAGGCCCTTCATTACGCGGAAAAGTTCCAATTCGTAATCAGGAAAATGAAATCATTGGTGTAGTGTCTGTTGGATTTTCA
ATGGATGATATTCATGGAGCGGTAGAAGTGTATGGAAAGCGTGTGTTTTGGATTACAATAATAGGTCTATTAATTGGAGT
AATAGGCTCTATATATTTAGCTGGCAGTATAAAAAGAATGATGTTTGGAATGGAGCCAGAAGAAATTTCATCTTTATATG
AAGAGCATAGTACAGTGATTCAATCTGTACGTGAAGGGATTATTGTCATTGATCAAAATGGCATGATTAGTTTAGTCAAT
CAAGCAGCTTATGATATTCTTTCGCTCGATGAACAACGAAATATTATTGGAGAATTTATTTTAGATATAATTCCTAATTC
AACGATATTAGATGTACTTCAAACTGGTGAAGAACAGTTTGATCGTCAATTAAATATAAAAGGGCAGGCTGTTATTGCGA
ACCGTCTACCCATTAAAGTGAAAAATAAAGTAACTGGTGTTGTATCGAGCTTGCGTTTGAAATCTGAAATGGATCAGTTA
ACAGCAGAATTATCTCAGACGAAGCAATATACAGAGGCATTACGAGCACAAACACATGAATATAACAATTTGTTGTATAC
GCTTTCAGGTCTTATCCAGCTAGAATTATATGAAGATGCTTTGGAGCTTATTCATAAGGAAACAGCGGTATATCAAGATT
TTGTTCAATTTATTATGAAACGAATTCAAAATCCATGGTTAGGTGGGATTTTAATTGGATTCTATAATAGAGCAAGAGAG
TTGAAGATTGATTTTATGCTAGATAGAGAAAGTAGCTTAGACAAATTAAGCCCGCATATTGAGAGTAATTATGTCGTTTC
TATTTTAGGAAATTTAATTACGAATGCATTTGAAGCGATTGAAAGAAATGAAGAACATGATAAGAAGGTAAGAATGTTTG
TAACTGATATCGGAGAAGAAATAGTAATTGAAGTGGAAGATTCAGGGCAAGGGGTTCATGATGAAATAATTACTAGCATA
TTCTATAAAGGCTTTTCGACGAAAGAAGGCGGGAAGAGAGGATATGGATTAACAAAAGTGAAAGAGTTAGTAGAAGATTT
AAATGGGAGTATTGCAATCGAAAAAGGGGATTTAGGTGGTGCATTGTTTATTATTGCATTACCAAAAGAGAGAGGCGATT
TGTAA

Upstream 100 bases:

>100_bases
AAAAAGACAGAAATATAGAGTAACCGTGTTATGATTAAGTATAATTGAAGAGACTGTATATTCTTTAAATTCTATCTTTA
ATCATGTTAGGGGAATGGTT

Downstream 100 bases:

>100_bases
TGGGTGAGGAGATTGAAGTATTGATTGTAGAAGATGATATTCGAATTGCGGATATTCATCGTCGTTTTACTGAAAAGATT
GAGGGATTTAAAGTAATTGG

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MKFKKLKLQPRITLTISALILVVLLLTSYLFYYILSETVEEQIGKRALHVAKTVAAIPEIKEAFQTENPASIIQPIAEKI
RMDTDADFIVVGNKEGIRYAHPRKDKIGEAMVGGDNKGVLLEGKSYVSKATGSLGPSLRGKVPIRNQENEIIGVVSVGFS
MDDIHGAVEVYGKRVFWITIIGLLIGVIGSIYLAGSIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDQNGMISLVN
QAAYDILSLDEQRNIIGEFILDIIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPIKVKNKVTGVVSSLRLKSEMDQL
TAELSQTKQYTEALRAQTHEYNNLLYTLSGLIQLELYEDALELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRARE
LKIDFMLDRESSLDKLSPHIESNYVVSILGNLITNAFEAIERNEEHDKKVRMFVTDIGEEIVIEVEDSGQGVHDEIITSI
FYKGFSTKEGGKRGYGLTKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGDL

Sequences:

>Translated_534_residues
MKFKKLKLQPRITLTISALILVVLLLTSYLFYYILSETVEEQIGKRALHVAKTVAAIPEIKEAFQTENPASIIQPIAEKI
RMDTDADFIVVGNKEGIRYAHPRKDKIGEAMVGGDNKGVLLEGKSYVSKATGSLGPSLRGKVPIRNQENEIIGVVSVGFS
MDDIHGAVEVYGKRVFWITIIGLLIGVIGSIYLAGSIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDQNGMISLVN
QAAYDILSLDEQRNIIGEFILDIIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPIKVKNKVTGVVSSLRLKSEMDQL
TAELSQTKQYTEALRAQTHEYNNLLYTLSGLIQLELYEDALELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRARE
LKIDFMLDRESSLDKLSPHIESNYVVSILGNLITNAFEAIERNEEHDKKVRMFVTDIGEEIVIEVEDSGQGVHDEIITSI
FYKGFSTKEGGKRGYGLTKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGDL
>Mature_534_residues
MKFKKLKLQPRITLTISALILVVLLLTSYLFYYILSETVEEQIGKRALHVAKTVAAIPEIKEAFQTENPASIIQPIAEKI
RMDTDADFIVVGNKEGIRYAHPRKDKIGEAMVGGDNKGVLLEGKSYVSKATGSLGPSLRGKVPIRNQENEIIGVVSVGFS
MDDIHGAVEVYGKRVFWITIIGLLIGVIGSIYLAGSIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDQNGMISLVN
QAAYDILSLDEQRNIIGEFILDIIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPIKVKNKVTGVVSSLRLKSEMDQL
TAELSQTKQYTEALRAQTHEYNNLLYTLSGLIQLELYEDALELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRARE
LKIDFMLDRESSLDKLSPHIESNYVVSILGNLITNAFEAIERNEEHDKKVRMFVTDIGEEIVIEVEDSGQGVHDEIITSI
FYKGFSTKEGGKRGYGLTKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGDL

Specific function: Member of the two-component regulatory system CitT/CitS. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786837, Length=535, Percent_Identity=32.7102803738318, Blast_Score=286, Evalue=3e-78,
Organism=Escherichia coli, GI1790567, Length=533, Percent_Identity=31.5196998123827, Blast_Score=278, Evalue=4e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59638; Mature: 59638

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKKLKLQPRITLTISALILVVLLLTSYLFYYILSETVEEQIGKRALHVAKTVAAIPEI
CCCCCEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEAFQTENPASIIQPIAEKIRMDTDADFIVVGNKEGIRYAHPRKDKIGEAMVGGDNKGVL
HHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHCCCHHCCCCCCCEE
LEGKSYVSKATGSLGPSLRGKVPIRNQENEIIGVVSVGFSMDDIHGAVEVYGKRVFWITI
EECHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHHHH
IGLLIGVIGSIYLAGSIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDQNGMISLVN
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHH
QAAYDILSLDEQRNIIGEFILDIIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPIKV
HHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCCCCEEEECCCCEEE
KNKVTGVVSSLRLKSEMDQLTAELSQTKQYTEALRAQTHEYNNLLYTLSGLIQLELYEDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRARELKIDFMLDRESSLDKLSPHI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEEECCCCCHHHCCCCC
ESNYVVSILGNLITNAFEAIERNEEHDKKVRMFVTDIGEEIVIEVEDSGQGVHDEIITSI
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHH
FYKGFSTKEGGKRGYGLTKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGDL
HHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MKFKKLKLQPRITLTISALILVVLLLTSYLFYYILSETVEEQIGKRALHVAKTVAAIPEI
CCCCCEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEAFQTENPASIIQPIAEKIRMDTDADFIVVGNKEGIRYAHPRKDKIGEAMVGGDNKGVL
HHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHCCCHHCCCCCCCEE
LEGKSYVSKATGSLGPSLRGKVPIRNQENEIIGVVSVGFSMDDIHGAVEVYGKRVFWITI
EECHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHHHH
IGLLIGVIGSIYLAGSIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDQNGMISLVN
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHH
QAAYDILSLDEQRNIIGEFILDIIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPIKV
HHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCCCCEEEECCCCEEE
KNKVTGVVSSLRLKSEMDQLTAELSQTKQYTEALRAQTHEYNNLLYTLSGLIQLELYEDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRARELKIDFMLDRESSLDKLSPHI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEEECCCCCHHHCCCCC
ESNYVVSILGNLITNAFEAIERNEEHDKKVRMFVTDIGEEIVIEVEDSGQGVHDEIITSI
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHH
FYKGFSTKEGGKRGYGLTKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGDL
HHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]