Click here to switch to the map view.

The map label for this gene is dnaB1 [H]

Identifier: 218901701

GI number: 218901701

Start: 535379

End: 536701

Strand: Direct

Name: dnaB1 [H]

Synonym: BCAH820_0562

Alternate gene names: 218901701

Gene position: 535379-536701 (Clockwise)

Preceding gene: 218901700

Following gene: 218901702

Centisome position: 10.1

GC content: 37.87

Gene sequence:

>1323_bases
ATGAGTAACGATATGATTCGTAACGTAGAAGCTGAACAAAGTGTTTTAGGTAGCATTATTCAAGAAGGCGATTTAATTAA
AGACTGTCAGTTAAAGGCAAAGCAGTTTTCCTTGCCGACACATCAAATGATTTTTAAAGCGATGAGAGAGCTAGAGGATG
ATGAGGCTCCGATAGATCTTGTTGCTCTCATTGGGAAATTCGATGAAAGTTTTATGCATCAAATTGGTGGGATTGAATTC
TTTGTAAACTTGACAGAAGTTGTAACTACAACTAAAAACTTTTCGTATCACGAAGGGCTAGTGATTGAAGCATGGAAGAT
GAGACACGCTCAGGATGTTGCTGGTAATTTATATAACCGTCTTCAGCAAGATAGGGATATAAGCGTAATTAGTGCCTCGA
TTGATGAACTAAGCGCCATTGAAGAAACAGGTTATTCCGATGAATTTAACTTGAAGGAAACGCTTGTTGATCTGTATAAA
AAAATGCAAATTGATGTAGGAGATTTAACTGGCATTCCAACTGGTTACGACGACTTGAACAGAATGACAGCAGGGCTACA
AGAAGGTGATTTAATCATTGTTGGCGCCCGTCCTTCGATGGGGAAAACAGCATTTGTATTAAACGTTGCTTTTCATGCAG
CAAGTGCTCAAACAGCAACAGGATTCTTCTCGCTAGAGATGGGAGAAGAACAGTTACTTAAGCGAATGATCTCAAGTACT
GGAAATATAGATGCCACGAAATTAAAGAATCCTAAAAAGCTATGTAATTTAAAGGACTGGGAAAAAATTAGCCAAGCAAT
GGGATTAATTAATGATTTGCCGCTAGAAATTTACGATAAAGCCAATGTAACAATGCAAGAGATTTACGCAAAGACTAGGA
AACTAAAGCGTAAATATCCAGAAAAAAAGGTGCTGGTCGCAATTGATTACTTGCAGCTTATTGTAGGAGATCCAAAGCAT
AAAGGGAATCGCATGCAAGAGATTGGTGAGATTAGCCGTAAGCTAAAACTTATGGCAAGAGAACTAAATGTATGTGTCGT
TGCATTATCGCAGTTAAGCCGTGCTGTAGAGAGTAGGCAAGATAAGAGACCATTACTATCAGACTTGCGTGAGAATGGTC
AAATCGAACAAGATGCGGACTTAATCGCATTCTTATATCGAGAAGATTATTACGACCGTGAAACGGAAAATAAAAACATT
ACAGAAATTATTTTAGCGAAACAAAGGAATGGTCCAGTAGGTGTTGTGGAATTAGCATTCATTAAAGAATTTAGTAAGTT
TGTAGGCTTAGAACGAAAGTTTGATAGACAACAGGAGGCTTAA

Upstream 100 bases:

>100_bases
CTACTAAAAACGTAATACAGCAGCAGGAAGCATGGGAACAAAACATTCCAACTGATGAAGAACTTGCAGCACTTACTAAA
CAGAGTGGGTGGATGGTTCA

Downstream 100 bases:

>100_bases
TCATGTTGTTACGTCAGGAGGTAGAACGTAGGAAGCTAACAATTATTCGTAAATTATTAGGCCTTGGATTATCGGAAATT
AATGGACAGACATTAGATAA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSNDMIRNVEAEQSVLGSIIQEGDLIKDCQLKAKQFSLPTHQMIFKAMRELEDDEAPIDLVALIGKFDESFMHQIGGIEF
FVNLTEVVTTTKNFSYHEGLVIEAWKMRHAQDVAGNLYNRLQQDRDISVISASIDELSAIEETGYSDEFNLKETLVDLYK
KMQIDVGDLTGIPTGYDDLNRMTAGLQEGDLIIVGARPSMGKTAFVLNVAFHAASAQTATGFFSLEMGEEQLLKRMISST
GNIDATKLKNPKKLCNLKDWEKISQAMGLINDLPLEIYDKANVTMQEIYAKTRKLKRKYPEKKVLVAIDYLQLIVGDPKH
KGNRMQEIGEISRKLKLMARELNVCVVALSQLSRAVESRQDKRPLLSDLRENGQIEQDADLIAFLYREDYYDRETENKNI
TEIILAKQRNGPVGVVELAFIKEFSKFVGLERKFDRQQEA

Sequences:

>Translated_440_residues
MSNDMIRNVEAEQSVLGSIIQEGDLIKDCQLKAKQFSLPTHQMIFKAMRELEDDEAPIDLVALIGKFDESFMHQIGGIEF
FVNLTEVVTTTKNFSYHEGLVIEAWKMRHAQDVAGNLYNRLQQDRDISVISASIDELSAIEETGYSDEFNLKETLVDLYK
KMQIDVGDLTGIPTGYDDLNRMTAGLQEGDLIIVGARPSMGKTAFVLNVAFHAASAQTATGFFSLEMGEEQLLKRMISST
GNIDATKLKNPKKLCNLKDWEKISQAMGLINDLPLEIYDKANVTMQEIYAKTRKLKRKYPEKKVLVAIDYLQLIVGDPKH
KGNRMQEIGEISRKLKLMARELNVCVVALSQLSRAVESRQDKRPLLSDLRENGQIEQDADLIAFLYREDYYDRETENKNI
TEIILAKQRNGPVGVVELAFIKEFSKFVGLERKFDRQQEA
>Mature_439_residues
SNDMIRNVEAEQSVLGSIIQEGDLIKDCQLKAKQFSLPTHQMIFKAMRELEDDEAPIDLVALIGKFDESFMHQIGGIEFF
VNLTEVVTTTKNFSYHEGLVIEAWKMRHAQDVAGNLYNRLQQDRDISVISASIDELSAIEETGYSDEFNLKETLVDLYKK
MQIDVGDLTGIPTGYDDLNRMTAGLQEGDLIIVGARPSMGKTAFVLNVAFHAASAQTATGFFSLEMGEEQLLKRMISSTG
NIDATKLKNPKKLCNLKDWEKISQAMGLINDLPLEIYDKANVTMQEIYAKTRKLKRKYPEKKVLVAIDYLQLIVGDPKHK
GNRMQEIGEISRKLKLMARELNVCVVALSQLSRAVESRQDKRPLLSDLRENGQIEQDADLIAFLYREDYYDRETENKNIT
EIILAKQRNGPVGVVELAFIKEFSKFVGLERKFDRQQEA

Specific function: Participates In Initiation And Elongation During Chromosome Replication; It Exhibits DNA-Dependent ATPase Activity And Contains Distinct Active Sites For ATP Binding, DNA Binding, And Interaction With DNAc Protein, Primase, And Other Prepriming Proteins.

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=435, Percent_Identity=38.8505747126437, Blast_Score=265, Evalue=6e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 49917; Mature: 49786

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDMIRNVEAEQSVLGSIIQEGDLIKDCQLKAKQFSLPTHQMIFKAMRELEDDEAPIDL
CCCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHH
VALIGKFDESFMHQIGGIEFFVNLTEVVTTTKNFSYHEGLVIEAWKMRHAQDVAGNLYNR
HHHHHHHHHHHHHHHCCHHHEEEHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHH
LQQDRDISVISASIDELSAIEETGYSDEFNLKETLVDLYKKMQIDVGDLTGIPTGYDDLN
HHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCCCCHHHHH
RMTAGLQEGDLIIVGARPSMGKTAFVLNVAFHAASAQTATGFFSLEMGEEQLLKRMISST
HHHCCCCCCCEEEEECCCCCCCCEEEEEHHHHHCCCCHHCCEEEEECCHHHHHHHHHHCC
GNIDATKLKNPKKLCNLKDWEKISQAMGLINDLPLEIYDKANVTMQEIYAKTRKLKRKYP
CCCCHHHCCCHHHHCCCHHHHHHHHHHHHHHCCCHHEECCCCCHHHHHHHHHHHHHHHCC
EKKVLVAIDYLQLIVGDPKHKGNRMQEIGEISRKLKLMARELNVCVVALSQLSRAVESRQ
CCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKRPLLSDLRENGQIEQDADLIAFLYREDYYDRETENKNITEIILAKQRNGPVGVVELAF
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHEEEECCCCCCCHHHHHH
IKEFSKFVGLERKFDRQQEA
HHHHHHHHCCHHHHHHHCCC
>Mature Secondary Structure 
SNDMIRNVEAEQSVLGSIIQEGDLIKDCQLKAKQFSLPTHQMIFKAMRELEDDEAPIDL
CCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHH
VALIGKFDESFMHQIGGIEFFVNLTEVVTTTKNFSYHEGLVIEAWKMRHAQDVAGNLYNR
HHHHHHHHHHHHHHHCCHHHEEEHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHH
LQQDRDISVISASIDELSAIEETGYSDEFNLKETLVDLYKKMQIDVGDLTGIPTGYDDLN
HHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCCCCHHHHH
RMTAGLQEGDLIIVGARPSMGKTAFVLNVAFHAASAQTATGFFSLEMGEEQLLKRMISST
HHHCCCCCCCEEEEECCCCCCCCEEEEEHHHHHCCCCHHCCEEEEECCHHHHHHHHHHCC
GNIDATKLKNPKKLCNLKDWEKISQAMGLINDLPLEIYDKANVTMQEIYAKTRKLKRKYP
CCCCHHHCCCHHHHCCCHHHHHHHHHHHHHHCCCHHEECCCCCHHHHHHHHHHHHHHHCC
EKKVLVAIDYLQLIVGDPKHKGNRMQEIGEISRKLKLMARELNVCVVALSQLSRAVESRQ
CCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKRPLLSDLRENGQIEQDADLIAFLYREDYYDRETENKNITEIILAKQRNGPVGVVELAF
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHEEEECCCCCCCHHHHHH
IKEFSKFVGLERKFDRQQEA
HHHHHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1312531 [H]