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The map label for this gene is cspR [H]

Identifier: 218901687

GI number: 218901687

Start: 522949

End: 523437

Strand: Direct

Name: cspR [H]

Synonym: BCAH820_0548

Alternate gene names: 218901687

Gene position: 522949-523437 (Clockwise)

Preceding gene: 218901686

Following gene: 218901688

Centisome position: 9.86

GC content: 33.54

Gene sequence:

>489_bases
GTGGGAGTACATGTTGTTTTATATCAACCAGAAATTCCGGCAAATACGGGAAATATTGCTCGTACTTGTGCAGCAACTGG
AACAGAATTACATTTGATTAGACCGCTTGGATTTTCAACGGATGATAAAATGTTAAAGCGTGCAGGATTAGATTATTGGC
AGCACGTGAAAATTACGTATTATGATTCAATTGAAGAGTTTTACGAGAAAAATAAAGACGGTGAATTCTTCTATTTAACA
AAGTATGGTGAGAAAGCTCATACAGCGTTTGATTATAGCAAACGTGAGAAGGATTACTATTTTGTATTTGGAAGAGAAAC
AAACGGCTTACCAGCTAATGTAATCGAAGAAAACTTCGATCATTGTTTACGCATTCCAATGACTGATAAAGTGCGTTCAT
TAAATTTATCTAATACAGCAGCTATTTTAATTTATGAAGCATTCCGTCAACAAAATTATCCAGGATTAGATTTAGAAATT
GTTTATTAA

Upstream 100 bases:

>100_bases
CCACAAATGAAATATAAATTCTTTCATATTATTAATGGGTAGAGATTTTTCGCTCAAGTGGTATAATACGTAGTAGACAA
AAAACAGAGGTGAATATCGC

Downstream 100 bases:

>100_bases
AAGTCGCCATATGGCGACTTTTTTTCTAAAAATAAAAATAATATATAATCATACATTTAAAAAAGGGTACATATTCATAT
AAAATGGGATATATGAATAA

Product: RNA methyltransferase, TrmH family, group 2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MGVHVVLYQPEIPANTGNIARTCAATGTELHLIRPLGFSTDDKMLKRAGLDYWQHVKITYYDSIEEFYEKNKDGEFFYLT
KYGEKAHTAFDYSKREKDYYFVFGRETNGLPANVIEENFDHCLRIPMTDKVRSLNLSNTAAILIYEAFRQQNYPGLDLEI
VY

Sequences:

>Translated_162_residues
MGVHVVLYQPEIPANTGNIARTCAATGTELHLIRPLGFSTDDKMLKRAGLDYWQHVKITYYDSIEEFYEKNKDGEFFYLT
KYGEKAHTAFDYSKREKDYYFVFGRETNGLPANVIEENFDHCLRIPMTDKVRSLNLSNTAAILIYEAFRQQNYPGLDLEI
VY
>Mature_161_residues
GVHVVLYQPEIPANTGNIARTCAATGTELHLIRPLGFSTDDKMLKRAGLDYWQHVKITYYDSIEEFYEKNKDGEFFYLTK
YGEKAHTAFDYSKREKDYYFVFGRETNGLPANVIEENFDHCLRIPMTDKVRSLNLSNTAAILIYEAFRQQNYPGLDLEIV
Y

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=154, Percent_Identity=46.1038961038961, Blast_Score=147, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18827; Mature: 18696

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVHVVLYQPEIPANTGNIARTCAATGTELHLIRPLGFSTDDKMLKRAGLDYWQHVKITY
CCEEEEEECCCCCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHCCCCHHEEEEEE
YDSIEEFYEKNKDGEFFYLTKYGEKAHTAFDYSKREKDYYFVFGRETNGLPANVIEENFD
EHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCCC
HCLRIPMTDKVRSLNLSNTAAILIYEAFRQQNYPGLDLEIVY
CEEECCCCCCCEECCCCCCEEEEEEEHHHHCCCCCCEEEEEC
>Mature Secondary Structure 
GVHVVLYQPEIPANTGNIARTCAATGTELHLIRPLGFSTDDKMLKRAGLDYWQHVKITY
CEEEEEECCCCCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHCCCCHHEEEEEE
YDSIEEFYEKNKDGEFFYLTKYGEKAHTAFDYSKREKDYYFVFGRETNGLPANVIEENFD
EHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCCC
HCLRIPMTDKVRSLNLSNTAAILIYEAFRQQNYPGLDLEIVY
CEEECCCCCCCEECCCCCCEEEEEEEHHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]