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The map label for this gene is gatA [H]

Identifier: 218901511

GI number: 218901511

Start: 336016

End: 337473

Strand: Direct

Name: gatA [H]

Synonym: BCAH820_0353

Alternate gene names: 218901511

Gene position: 336016-337473 (Clockwise)

Preceding gene: 218901510

Following gene: 218901512

Centisome position: 6.34

GC content: 40.26

Gene sequence:

>1458_bases
ATGTCATTATTTGATCATTCAGTATCAGAGTTACATAAGAAGTTAAACAACAAAGAAATTTCCGTTACGGATTTAGTAGA
AGAATCTTACAAACGTATTGCGGATGTTGAAGATAACGTAAAAGCTTTTCTTACATTAGATGAAGAAAACGCACGCGCGA
AAGCGAAAGAATTAGACGCAAAAATTGGCGCTGAAGATAATGGTTTATTATTTGGTATGCCAATCGGTGTTAAAGATAAC
ATTGTAACTAACGGGCTTCGTACAACTTGTGCGAGCAAAATATTAGCAAACTTCGATCCAATTTATGATGCGACAGTTGT
GCAAAAGCTAAAAGCTGCTGACACAATTACAATCGGTAAATTAAATATGGATGAGTTCGCAATGGGTTCTTCAAATGAAA
ACTCAGGTTTCTACGCTACGAAAAATCCATGGAACTTAGATTACGTTCCAGGCGGATCTAGTGGTGGTTCTGCAGCAGCT
GTAGCAGCAGGAGAAGTATTATTCTCTCTAGGTTCTGATACGGGTGGTTCTATCCGTCAGCCAGCTGCATATTGCGGCGT
TGTAGGTTTAAAACCAACTTACGGACGCGTATCTCGTTACGGATTAGTAGCATTCGCATCTTCACTTGACCAAATCGGAC
CGATTACACGTACAGTAGAAGACAATGCATACTTATTACAAGCTATTTCAGGCCTTGACCGTATGGATGCAACTTCTGCA
AACGTTGAAGTAGGAAACTACTTAGCTGGTTTAACAGGCGACGTTAAAGGTTTACGCATTGCTGTACCGAAAGAATACTT
AGGTGAAGGTGTTGGCGAGGAAGCCCGTGAGTCAGTGCTAGCTGCTTTAAAAGTATTAGAAGGTATGGGCGCAACTTGGG
AGGAAGTATCTCTTCCACACTCTAAATACGCTCTAGCAACGTATTACTTACTATCTTCTTCTGAAGCATCTGCTAACCTT
TCACGCTTCGACGGCGTACGTTACGGCGTTCGTTCTGACAATGTGAATAACTTAATGGATCTTTACAAAAACACACGTAG
CGAAGGCTTCGGTGATGAAGTTAAACGTCGTATTATGCTTGGTACATTTGCACTTAGCTCTGGTTACTATGATGCATATT
ACAAAAAGGCACAACAAGTACGTACATTAATTAAAAACGACTTTGAAAATGTATTTGCTAACTACGATGTTATTATTGGA
CCAACTACGCCAACTCCGGCATTTAAAGTGGGCGAAAAAGTTGATGATCCAATGACAATGTATGCAAATGACATTTTAAC
AATCCCAGTAAACTTAGCTGGTGTTCCAGCGATTTCTGTTCCATGTGGATTCGGTGCTAACAACATGCCACTTGGTCTAC
AAATCATTGGTAAACACTTCGATGAAGCGACAATTTACCGCGTTGCACATGCGTTTGAGCAAGCAACAGACTATCATACA
AAAAAAGCAAGTCTGTAA

Upstream 100 bases:

>100_bases
AAAAGGTTTACCAGTTGAAGAAGTATTAAAAAATGCACCGGATCACAAAGATAATCAAATCCGTGTTCCAGCAGTATTAG
AATAGAGGAGGGGGATTTCG

Downstream 100 bases:

>100_bases
GGAGGCGAGCATAGATGAATTTAGAAACAATTATTGGTTTAGAGGTTCACGTTGAGTTAAAAACAAATTCGAAAATTTTC
TCTGCGAGTCCAACAGAATT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: ADP; phosphate; Gln-tRNA(Gln); L-glutamate

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSLFDHSVSELHKKLNNKEISVTDLVEESYKRIADVEDNVKAFLTLDEENARAKAKELDAKIGAEDNGLLFGMPIGVKDN
IVTNGLRTTCASKILANFDPIYDATVVQKLKAADTITIGKLNMDEFAMGSSNENSGFYATKNPWNLDYVPGGSSGGSAAA
VAAGEVLFSLGSDTGGSIRQPAAYCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITRTVEDNAYLLQAISGLDRMDATSA
NVEVGNYLAGLTGDVKGLRIAVPKEYLGEGVGEEARESVLAALKVLEGMGATWEEVSLPHSKYALATYYLLSSSEASANL
SRFDGVRYGVRSDNVNNLMDLYKNTRSEGFGDEVKRRIMLGTFALSSGYYDAYYKKAQQVRTLIKNDFENVFANYDVIIG
PTTPTPAFKVGEKVDDPMTMYANDILTIPVNLAGVPAISVPCGFGANNMPLGLQIIGKHFDEATIYRVAHAFEQATDYHT
KKASL

Sequences:

>Translated_485_residues
MSLFDHSVSELHKKLNNKEISVTDLVEESYKRIADVEDNVKAFLTLDEENARAKAKELDAKIGAEDNGLLFGMPIGVKDN
IVTNGLRTTCASKILANFDPIYDATVVQKLKAADTITIGKLNMDEFAMGSSNENSGFYATKNPWNLDYVPGGSSGGSAAA
VAAGEVLFSLGSDTGGSIRQPAAYCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITRTVEDNAYLLQAISGLDRMDATSA
NVEVGNYLAGLTGDVKGLRIAVPKEYLGEGVGEEARESVLAALKVLEGMGATWEEVSLPHSKYALATYYLLSSSEASANL
SRFDGVRYGVRSDNVNNLMDLYKNTRSEGFGDEVKRRIMLGTFALSSGYYDAYYKKAQQVRTLIKNDFENVFANYDVIIG
PTTPTPAFKVGEKVDDPMTMYANDILTIPVNLAGVPAISVPCGFGANNMPLGLQIIGKHFDEATIYRVAHAFEQATDYHT
KKASL
>Mature_484_residues
SLFDHSVSELHKKLNNKEISVTDLVEESYKRIADVEDNVKAFLTLDEENARAKAKELDAKIGAEDNGLLFGMPIGVKDNI
VTNGLRTTCASKILANFDPIYDATVVQKLKAADTITIGKLNMDEFAMGSSNENSGFYATKNPWNLDYVPGGSSGGSAAAV
AAGEVLFSLGSDTGGSIRQPAAYCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITRTVEDNAYLLQAISGLDRMDATSAN
VEVGNYLAGLTGDVKGLRIAVPKEYLGEGVGEEARESVLAALKVLEGMGATWEEVSLPHSKYALATYYLLSSSEASANLS
RFDGVRYGVRSDNVNNLMDLYKNTRSEGFGDEVKRRIMLGTFALSSGYYDAYYKKAQQVRTLIKNDFENVFANYDVIIGP
TTPTPAFKVGEKVDDPMTMYANDILTIPVNLAGVPAISVPCGFGANNMPLGLQIIGKHFDEATIYRVAHAFEQATDYHTK
KASL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=501, Percent_Identity=38.3233532934132, Blast_Score=320, Evalue=3e-87,
Organism=Homo sapiens, GI195972892, Length=487, Percent_Identity=25.6673511293634, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI166795287, Length=161, Percent_Identity=34.1614906832298, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=442, Percent_Identity=37.10407239819, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17537465, Length=501, Percent_Identity=25.9481037924152, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17556264, Length=187, Percent_Identity=35.2941176470588, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17538252, Length=517, Percent_Identity=22.8239845261122, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=156, Percent_Identity=32.6923076923077, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=170, Percent_Identity=35.2941176470588, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=170, Percent_Identity=35.2941176470588, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538254, Length=227, Percent_Identity=29.5154185022026, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=462, Percent_Identity=35.0649350649351, Blast_Score=243, Evalue=6e-65,
Organism=Saccharomyces cerevisiae, GI6319685, Length=413, Percent_Identity=30.7506053268765, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6320448, Length=408, Percent_Identity=25.7352941176471, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24648113, Length=502, Percent_Identity=37.0517928286853, Blast_Score=291, Evalue=5e-79,
Organism=Drosophila melanogaster, GI24652985, Length=496, Percent_Identity=26.4112903225806, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19922090, Length=496, Percent_Identity=26.4112903225806, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24652981, Length=496, Percent_Identity=26.4112903225806, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24652983, Length=496, Percent_Identity=26.4112903225806, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161078093, Length=509, Percent_Identity=26.9155206286837, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24644968, Length=505, Percent_Identity=26.3366336633663, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24648435, Length=492, Percent_Identity=24.5934959349593, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24648437, Length=492, Percent_Identity=24.5934959349593, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI45550774, Length=492, Percent_Identity=24.5934959349593, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI21356731, Length=499, Percent_Identity=24.0480961923848, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648441, Length=417, Percent_Identity=25.1798561151079, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648439, Length=417, Percent_Identity=25.1798561151079, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.7

Molecular weight: Translated: 52322; Mature: 52190

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFDHSVSELHKKLNNKEISVTDLVEESYKRIADVEDNVKAFLTLDEENARAKAKELDA
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHH
KIGAEDNGLLFGMPIGVKDNIVTNGLRTTCASKILANFDPIYDATVVQKLKAADTITIGK
HCCCCCCCEEEEECCCCCCCHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEEC
LNMDEFAMGSSNENSGFYATKNPWNLDYVPGGSSGGSAAAVAAGEVLFSLGSDTGGSIRQ
CCHHHHCCCCCCCCCCEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
PAAYCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITRTVEDNAYLLQAISGLDRMDATSA
CHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHCCHHCCCCCC
NVEVGNYLAGLTGDVKGLRIAVPKEYLGEGVGEEARESVLAALKVLEGMGATWEEVSLPH
CEEHHHHHHCCCCCCCCEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCC
SKYALATYYLLSSSEASANLSRFDGVRYGVRSDNVNNLMDLYKNTRSEGFGDEVKRRIML
CHHHHEEEEEEECCCCCCCHHHHCCEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
GTFALSSGYYDAYYKKAQQVRTLIKNDFENVFANYDVIIGPTTPTPAFKVGEKVDDPMTM
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHCCCCCCCCHHH
YANDILTIPVNLAGVPAISVPCGFGANNMPLGLQIIGKHFDEATIYRVAHAFEQATDYHT
HCCCEEEEEECCCCCCEEECCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCH
KKASL
HCCCC
>Mature Secondary Structure 
SLFDHSVSELHKKLNNKEISVTDLVEESYKRIADVEDNVKAFLTLDEENARAKAKELDA
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHH
KIGAEDNGLLFGMPIGVKDNIVTNGLRTTCASKILANFDPIYDATVVQKLKAADTITIGK
HCCCCCCCEEEEECCCCCCCHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEEC
LNMDEFAMGSSNENSGFYATKNPWNLDYVPGGSSGGSAAAVAAGEVLFSLGSDTGGSIRQ
CCHHHHCCCCCCCCCCEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
PAAYCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITRTVEDNAYLLQAISGLDRMDATSA
CHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHCCHHCCCCCC
NVEVGNYLAGLTGDVKGLRIAVPKEYLGEGVGEEARESVLAALKVLEGMGATWEEVSLPH
CEEHHHHHHCCCCCCCCEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCC
SKYALATYYLLSSSEASANLSRFDGVRYGVRSDNVNNLMDLYKNTRSEGFGDEVKRRIML
CHHHHEEEEEEECCCCCCCHHHHCCEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
GTFALSSGYYDAYYKKAQQVRTLIKNDFENVFANYDVIIGPTTPTPAFKVGEKVDDPMTM
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHCCCCCCCCHHH
YANDILTIPVNLAGVPAISVPCGFGANNMPLGLQIIGKHFDEATIYRVAHAFEQATDYHT
HCCCEEEEEECCCCCCEEECCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCH
KKASL
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; Glu-tRNA(Gln); L-glutamine

Specific reaction: ATP + Glu-tRNA(Gln) + L-glutamine = ADP + phosphate + Gln-tRNA(Gln) + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA