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The map label for this gene is ddl [H]

Identifier: 218901438

GI number: 218901438

Start: 232661

End: 233746

Strand: Direct

Name: ddl [H]

Synonym: BCAH820_0257

Alternate gene names: 218901438

Gene position: 232661-233746 (Clockwise)

Preceding gene: 218901437

Following gene: 218901439

Centisome position: 4.39

GC content: 36.46

Gene sequence:

>1086_bases
GTGACAAAAATTAAATTAGGTTTATTATATGGTGGAAAATCAGCTGAGCATCAAGTTTCGTTACAAACGGCTCTTGCTGC
TATTAAAGCATTAAATCAAGATAAATTCGAGATTCATCCAATTTATATTACAGAACAAGGTCAATGGATACGCGGTGAAC
GTATTGAAGGTGAAGTAGCAAATGTTGAAGTTCTAAAAATGAGCGGTGCAGAAAATGCGATTTCTCCGTTATCTTTAAGC
ACAGAAATCATTCCATCTGCAAATGCTGAGGAAAATGCTATCGACGTTATTTTCCCATTACTACATGGACCAAATGGTGA
AGATGGAACGGTTCAAGGTTTATTAGAATTAATGAACATTCCTTATGTAGGAAATGGTGTTCTAGCATCTGCTGCTGGTA
TGGATAAAGTTGTTATGAAAAACATTTTCGCAGAAGCTGGATTAAAACAAGCGAAATATGCATCATTCATTCGTAGCGGA
TGGGAAAAAGATCGTGAAACAGCATATGAAAAAGTAGAAGAAGTATTAGGATACCCTTGCTTCGTAAAACCAGCAAACCT
TGGTTCAAGTGTTGGGATTAATAAGTGTAAAAATCGTGAAGAGCTTGAGAATGCATTTGAAGAGGCATTCCAATTTGACC
GTAAAATTATTGTAGAAGAGAATATCATTGGACGTGAAGTAGAAGTTGGTGTACTAGGTAATGATGAACCGAAATGTTCA
GTTGTAGGTGAAATCGTACCTAAAAAAGACTTCTATGATTATAAGTCGAAATACATCGATGGTGATACAGCATTAATTAT
CCCAGCTGAAATGACAGAAGAAGAGTCTAATGTTATTAAGCGAGATGCGATTGTTGCCTTCCAATCGTTAGATGGTGCTG
GCTTAACGCGAGCTGATTTCTTCTTAACGAAAGATGGAGAAGTATATATTAACGAAGTGAATACGATGCCAGGATTTACG
CCGTTTAGTATGTTCCCTCTACTGTGGCAACATACTGGATTACCGTATCCGGAATTAATTGAAGAGCTAATTCGTTTAGC
AATGGAGCGTCACGAAGAAAAACAAAAAATTAAATATACAATCTAA

Upstream 100 bases:

>100_bases
ATTATTTGTGGAAGTTACATTTTATTTCAAATAGGAACTACCTATTTTTATAAAAAGTGCTAAAATAGAAAAATGAAAAC
AATTGGTTAGGGTGATTTCA

Downstream 100 bases:

>100_bases
CAAAAAAGGAGGAGGCACTATTCGCAGGAATAGTGCTTCCTCTATGTAAGGAGTGTTTTCATGATAAATCGAACGTTAAA
ACAAGTAGAACAAATGATAA

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MTKIKLGLLYGGKSAEHQVSLQTALAAIKALNQDKFEIHPIYITEQGQWIRGERIEGEVANVEVLKMSGAENAISPLSLS
TEIIPSANAEENAIDVIFPLLHGPNGEDGTVQGLLELMNIPYVGNGVLASAAGMDKVVMKNIFAEAGLKQAKYASFIRSG
WEKDRETAYEKVEEVLGYPCFVKPANLGSSVGINKCKNREELENAFEEAFQFDRKIIVEENIIGREVEVGVLGNDEPKCS
VVGEIVPKKDFYDYKSKYIDGDTALIIPAEMTEEESNVIKRDAIVAFQSLDGAGLTRADFFLTKDGEVYINEVNTMPGFT
PFSMFPLLWQHTGLPYPELIEELIRLAMERHEEKQKIKYTI

Sequences:

>Translated_361_residues
MTKIKLGLLYGGKSAEHQVSLQTALAAIKALNQDKFEIHPIYITEQGQWIRGERIEGEVANVEVLKMSGAENAISPLSLS
TEIIPSANAEENAIDVIFPLLHGPNGEDGTVQGLLELMNIPYVGNGVLASAAGMDKVVMKNIFAEAGLKQAKYASFIRSG
WEKDRETAYEKVEEVLGYPCFVKPANLGSSVGINKCKNREELENAFEEAFQFDRKIIVEENIIGREVEVGVLGNDEPKCS
VVGEIVPKKDFYDYKSKYIDGDTALIIPAEMTEEESNVIKRDAIVAFQSLDGAGLTRADFFLTKDGEVYINEVNTMPGFT
PFSMFPLLWQHTGLPYPELIEELIRLAMERHEEKQKIKYTI
>Mature_360_residues
TKIKLGLLYGGKSAEHQVSLQTALAAIKALNQDKFEIHPIYITEQGQWIRGERIEGEVANVEVLKMSGAENAISPLSLST
EIIPSANAEENAIDVIFPLLHGPNGEDGTVQGLLELMNIPYVGNGVLASAAGMDKVVMKNIFAEAGLKQAKYASFIRSGW
EKDRETAYEKVEEVLGYPCFVKPANLGSSVGINKCKNREELENAFEEAFQFDRKIIVEENIIGREVEVGVLGNDEPKCSV
VGEIVPKKDFYDYKSKYIDGDTALIIPAEMTEEESNVIKRDAIVAFQSLDGAGLTRADFFLTKDGEVYINEVNTMPGFTP
FSMFPLLWQHTGLPYPELIEELIRLAMERHEEKQKIKYTI

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=368, Percent_Identity=44.0217391304348, Blast_Score=308, Evalue=4e-85,
Organism=Escherichia coli, GI1786280, Length=343, Percent_Identity=32.0699708454811, Blast_Score=176, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 40128; Mature: 39997

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIKLGLLYGGKSAEHQVSLQTALAAIKALNQDKFEIHPIYITEQGQWIRGERIEGEVA
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCEECCCCCCCCCC
NVEVLKMSGAENAISPLSLSTEIIPSANAEENAIDVIFPLLHGPNGEDGTVQGLLELMNI
CEEEEEECCCCCCCCCCCCCEEECCCCCCCCCHHEEEEEHHCCCCCCCHHHHHHHHHHCC
PYVGNGVLASAAGMDKVVMKNIFAEAGLKQAKYASFIRSGWEKDRETAYEKVEEVLGYPC
CCCCCCHHEHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FVKPANLGSSVGINKCKNREELENAFEEAFQFDRKIIVEENIIGREVEVGVLGNDEPKCS
EEECCCCCCCCCCHHCCCHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEECCCCCCEE
VVGEIVPKKDFYDYKSKYIDGDTALIIPAEMTEEESNVIKRDAIVAFQSLDGAGLTRADF
EHHHHCCCCCHHHHHHHCCCCCEEEEEEHHHCCCHHHHHHHHHHHEEECCCCCCCEEEEE
FLTKDGEVYINEVNTMPGFTPFSMFPLLWQHTGLPYPELIEELIRLAMERHEEKQKIKYT
EEECCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEECC
I
C
>Mature Secondary Structure 
TKIKLGLLYGGKSAEHQVSLQTALAAIKALNQDKFEIHPIYITEQGQWIRGERIEGEVA
CEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCEECCCCCCCCCC
NVEVLKMSGAENAISPLSLSTEIIPSANAEENAIDVIFPLLHGPNGEDGTVQGLLELMNI
CEEEEEECCCCCCCCCCCCCEEECCCCCCCCCHHEEEEEHHCCCCCCCHHHHHHHHHHCC
PYVGNGVLASAAGMDKVVMKNIFAEAGLKQAKYASFIRSGWEKDRETAYEKVEEVLGYPC
CCCCCCHHEHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FVKPANLGSSVGINKCKNREELENAFEEAFQFDRKIIVEENIIGREVEVGVLGNDEPKCS
EEECCCCCCCCCCHHCCCHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEECCCCCCEE
VVGEIVPKKDFYDYKSKYIDGDTALIIPAEMTEEESNVIKRDAIVAFQSLDGAGLTRADF
EHHHHCCCCCHHHHHHHCCCCCEEEEEEHHHCCCHHHHHHHHHHHEEECCCCCCCEEEEE
FLTKDGEVYINEVNTMPGFTPFSMFPLLWQHTGLPYPELIEELIRLAMERHEEKQKIKYT
EEECCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEECC
I
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA