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The map label for this gene is appA [H]

Identifier: 218901422

GI number: 218901422

Start: 219269

End: 220996

Strand: Direct

Name: appA [H]

Synonym: BCAH820_0241

Alternate gene names: 218901422

Gene position: 219269-220996 (Clockwise)

Preceding gene: 218901421

Following gene: 218901423

Centisome position: 4.14

GC content: 34.72

Gene sequence:

>1728_bases
ATGAAGAAAAAGACAAAAAAATGGGCTGGTGTATTTTCGGTATTACTGGGTAGTTCGCTTGTGTTATCTGCTTGTGGGGG
ACAGGAGGATACGGCTTCTACAGAACCAGTAAAAAAGCAAGACTTAAAAGACGCTAAGATCGAATCAATTCCAGCTACAG
ATAAAAAGAAAAGTCCAGAGAAAGCAAATCAGCGAAAGGATACTTTTATTACGGCCATTTCTAAGCCAGGGGGAGTTTTC
CTTCCATATTTCCAAGAGAATGGTTGGGATGGAAATGTAACGTCTGTAATTTTTGCGTCACTAGTAACAACAGACAAACA
AAGTAAACCTATTCCGGATCTTGCAGAAAAATGGGATATTTCTGCTGAGCAGTTAACATATACATTCCACTTACGTAAAA
ATTTAAAATTTAGTGATGGATCGCCTTTAACTGCAGATGACGTAGCATTTACGTTAACACTACTTCATGATAAAGCGTAT
GAAGGTGGATTGGATATTGCTCAGTATGCTGTTAAAGGCGGGAAAGAATATAAAGAAGGAAAAACGACTTCTATTGAAGG
GATTCAAGTTGTTGATCCACAGACAATTAAAATTACAACTGAAAAAGTTAATTCACAGGCGCTAACTAATTTAGGTGGCC
CAGTGCTATCAAAAGCTTATTATGGAAAAGATTATAAACAAAATACAAGTTTGGACTATTTAAAAGCATTATATGGGCAA
CCAATTGCAGCGGGTCCATATAAATTTGAAAAATATGTTCCAGGTCAAGAAGTACGCTTTGTTGCTAACGAACATTATTA
TGCAGGTAAACCAAAAATCAAAAACTTTATTTATAAAATCACATCAGGTGATACTGGATTCCAATTATTCCAAACAGGTG
AACTGGACTATAGTGGATTTAGAGCGAACCCTGAAAATATAGACCAATTAAAAGGATTAGAGTTTGCAAATATTAATCTT
GAATCATCAAGTGATATTGCTTATATCTATGTAAATAATAAGAAGTCGTATTTGAAAGATAAAAAGGTACGCCAAGCACT
TACTTATGGATTAGACCGTAAGAAGTATGTGGATACAGCTTTACAAGGATATGGCTCTGTTGCTAATGTACCAATTGCTC
CAGTTTCATGGGCATATACAGAAGAAGGTATTAATAAATATCCACACGACGTAGAAAAGGCTAAAAAATTATTGGATGAA
GCTGGTTGGAAAGTAGGTTCTGACGGGGTTCGTGAAAAAGATGGTCAAAAGTTAAAATTAACATACTATGCATCAAATAC
AGGTAAGACAAATGATATCTTTATTCCAATTGCAAAAGAAAGTTACAAAGAAATTGGTGTTGAATTAAATCCAGAGTTGA
TGGACTTTAATACGATGCTTTCAAAAGTAGGAAAAGGTGACTACGATTTAGCGGCAGTTTCAACACCAGGGATTAGTGAT
CCAAGTGAAGTTGTAAGTGAGTACTTATCAACTAATCCAAAAAGTGATACGGGTTATAATAATCCGAAAGTAGATGAGTT
GATTGTAAAAGGTATAGGAACGACAGATATTGAAAAACGTAAAGCGATTTATAAAGAGCTGTATAAAGAGTTAAGTGATG
ATCCACCAGTTATTTTATTAAACTATCGTAAACTACTTTATGCTCATAATGCACGTATAAAAGGAATTGATCCAGAAAAG
TACGATAGCATCAGTTCCAACTTACCAGTGTTATCTATTGAACAATAA

Upstream 100 bases:

>100_bases
TAAAACCGACTTTTCCGATTGGCTATAAACATGCTGTGGGAGGCATACATAAGCTAATAAGTTCAAAGGGTATCACTATT
AAAAAGTTAGGGTGGGGACA

Downstream 100 bases:

>100_bases
GGGTTGACCTAAATAATAGAAGACGTTTTTTCACACAGCAATGAAATGGTAGCTGTTTTGAAAAAATGTTCTTCTATTTT
TAAGTAAATAGGATTGGGGA

Product: putative oligopeptide ABC transporter, oligopeptide-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MKKKTKKWAGVFSVLLGSSLVLSACGGQEDTASTEPVKKQDLKDAKIESIPATDKKKSPEKANQRKDTFITAISKPGGVF
LPYFQENGWDGNVTSVIFASLVTTDKQSKPIPDLAEKWDISAEQLTYTFHLRKNLKFSDGSPLTADDVAFTLTLLHDKAY
EGGLDIAQYAVKGGKEYKEGKTTSIEGIQVVDPQTIKITTEKVNSQALTNLGGPVLSKAYYGKDYKQNTSLDYLKALYGQ
PIAAGPYKFEKYVPGQEVRFVANEHYYAGKPKIKNFIYKITSGDTGFQLFQTGELDYSGFRANPENIDQLKGLEFANINL
ESSSDIAYIYVNNKKSYLKDKKVRQALTYGLDRKKYVDTALQGYGSVANVPIAPVSWAYTEEGINKYPHDVEKAKKLLDE
AGWKVGSDGVREKDGQKLKLTYYASNTGKTNDIFIPIAKESYKEIGVELNPELMDFNTMLSKVGKGDYDLAAVSTPGISD
PSEVVSEYLSTNPKSDTGYNNPKVDELIVKGIGTTDIEKRKAIYKELYKELSDDPPVILLNYRKLLYAHNARIKGIDPEK
YDSISSNLPVLSIEQ

Sequences:

>Translated_575_residues
MKKKTKKWAGVFSVLLGSSLVLSACGGQEDTASTEPVKKQDLKDAKIESIPATDKKKSPEKANQRKDTFITAISKPGGVF
LPYFQENGWDGNVTSVIFASLVTTDKQSKPIPDLAEKWDISAEQLTYTFHLRKNLKFSDGSPLTADDVAFTLTLLHDKAY
EGGLDIAQYAVKGGKEYKEGKTTSIEGIQVVDPQTIKITTEKVNSQALTNLGGPVLSKAYYGKDYKQNTSLDYLKALYGQ
PIAAGPYKFEKYVPGQEVRFVANEHYYAGKPKIKNFIYKITSGDTGFQLFQTGELDYSGFRANPENIDQLKGLEFANINL
ESSSDIAYIYVNNKKSYLKDKKVRQALTYGLDRKKYVDTALQGYGSVANVPIAPVSWAYTEEGINKYPHDVEKAKKLLDE
AGWKVGSDGVREKDGQKLKLTYYASNTGKTNDIFIPIAKESYKEIGVELNPELMDFNTMLSKVGKGDYDLAAVSTPGISD
PSEVVSEYLSTNPKSDTGYNNPKVDELIVKGIGTTDIEKRKAIYKELYKELSDDPPVILLNYRKLLYAHNARIKGIDPEK
YDSISSNLPVLSIEQ
>Mature_575_residues
MKKKTKKWAGVFSVLLGSSLVLSACGGQEDTASTEPVKKQDLKDAKIESIPATDKKKSPEKANQRKDTFITAISKPGGVF
LPYFQENGWDGNVTSVIFASLVTTDKQSKPIPDLAEKWDISAEQLTYTFHLRKNLKFSDGSPLTADDVAFTLTLLHDKAY
EGGLDIAQYAVKGGKEYKEGKTTSIEGIQVVDPQTIKITTEKVNSQALTNLGGPVLSKAYYGKDYKQNTSLDYLKALYGQ
PIAAGPYKFEKYVPGQEVRFVANEHYYAGKPKIKNFIYKITSGDTGFQLFQTGELDYSGFRANPENIDQLKGLEFANINL
ESSSDIAYIYVNNKKSYLKDKKVRQALTYGLDRKKYVDTALQGYGSVANVPIAPVSWAYTEEGINKYPHDVEKAKKLLDE
AGWKVGSDGVREKDGQKLKLTYYASNTGKTNDIFIPIAKESYKEIGVELNPELMDFNTMLSKVGKGDYDLAAVSTPGISD
PSEVVSEYLSTNPKSDTGYNNPKVDELIVKGIGTTDIEKRKAIYKELYKELSDDPPVILLNYRKLLYAHNARIKGIDPEK
YDSISSNLPVLSIEQ

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=470, Percent_Identity=27.2340425531915, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1787762, Length=455, Percent_Identity=27.032967032967, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1789887, Length=502, Percent_Identity=23.9043824701195, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1787052, Length=497, Percent_Identity=25.9557344064386, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1787495, Length=485, Percent_Identity=24.1237113402062, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787551, Length=524, Percent_Identity=22.5190839694656, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87081878, Length=475, Percent_Identity=24, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1789397, Length=464, Percent_Identity=22.198275862069, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 63818; Mature: 63818

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKTKKWAGVFSVLLGSSLVLSACGGQEDTASTEPVKKQDLKDAKIESIPATDKKKSPE
CCCCHHHHHHHHHHHHCCHHEEECCCCCCCCCCCCCCHHHCCCCCHHCCCCCCCCCCCCH
KANQRKDTFITAISKPGGVFLPYFQENGWDGNVTSVIFASLVTTDKQSKPIPDLAEKWDI
HHHCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHCC
SAEQLTYTFHLRKNLKFSDGSPLTADDVAFTLTLLHDKAYEGGLDIAQYAVKGGKEYKEG
CHHHEEEEEEEECCCCCCCCCCCCHHHHEEEEEEHHHHCCCCCCHHHHHHHHCCCHHCCC
KTTSIEGIQVVDPQTIKITTEKVNSQALTNLGGPVLSKAYYGKDYKQNTSLDYLKALYGQ
CCCCCCCEEEECCCEEEEEHHHCCHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHCC
PIAAGPYKFEKYVPGQEVRFVANEHYYAGKPKIKNFIYKITSGDTGFQLFQTGELDYSGF
CCCCCCCCHHHCCCCCCEEEEECCCEECCCCHHCEEEEEEECCCCCEEEEEECCCCCCCC
RANPENIDQLKGLEFANINLESSSDIAYIYVNNKKSYLKDKKVRQALTYGLDRKKYVDTA
CCCHHHHHHHCCCEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHH
LQGYGSVANVPIAPVSWAYTEEGINKYPHDVEKAKKLLDEAGWKVGSDGVREKDGQKLKL
HHCCCCCCCCCCCCCEEHHHHHHHHCCCHHHHHHHHHHHHCCCEECCCCCCCCCCCEEEE
TYYASNTGKTNDIFIPIAKESYKEIGVELNPELMDFNTMLSKVGKGDYDLAAVSTPGISD
EEEECCCCCCCEEEEEECHHHHHHCCCEECCHHHHHHHHHHHHCCCCCCEEEECCCCCCC
PSEVVSEYLSTNPKSDTGYNNPKVDELIVKGIGTTDIEKRKAIYKELYKELSDDPPVILL
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
NYRKLLYAHNARIKGIDPEKYDSISSNLPVLSIEQ
CCHHHHHHCCCEEECCCCHHHHHHHCCCCEEEECC
>Mature Secondary Structure
MKKKTKKWAGVFSVLLGSSLVLSACGGQEDTASTEPVKKQDLKDAKIESIPATDKKKSPE
CCCCHHHHHHHHHHHHCCHHEEECCCCCCCCCCCCCCHHHCCCCCHHCCCCCCCCCCCCH
KANQRKDTFITAISKPGGVFLPYFQENGWDGNVTSVIFASLVTTDKQSKPIPDLAEKWDI
HHHCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHCC
SAEQLTYTFHLRKNLKFSDGSPLTADDVAFTLTLLHDKAYEGGLDIAQYAVKGGKEYKEG
CHHHEEEEEEEECCCCCCCCCCCCHHHHEEEEEEHHHHCCCCCCHHHHHHHHCCCHHCCC
KTTSIEGIQVVDPQTIKITTEKVNSQALTNLGGPVLSKAYYGKDYKQNTSLDYLKALYGQ
CCCCCCCEEEECCCEEEEEHHHCCHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHCC
PIAAGPYKFEKYVPGQEVRFVANEHYYAGKPKIKNFIYKITSGDTGFQLFQTGELDYSGF
CCCCCCCCHHHCCCCCCEEEEECCCEECCCCHHCEEEEEEECCCCCEEEEEECCCCCCCC
RANPENIDQLKGLEFANINLESSSDIAYIYVNNKKSYLKDKKVRQALTYGLDRKKYVDTA
CCCHHHHHHHCCCEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHH
LQGYGSVANVPIAPVSWAYTEEGINKYPHDVEKAKKLLDEAGWKVGSDGVREKDGQKLKL
HHCCCCCCCCCCCCCEEHHHHHHHHCCCHHHHHHHHHHHHCCCEECCCCCCCCCCCEEEE
TYYASNTGKTNDIFIPIAKESYKEIGVELNPELMDFNTMLSKVGKGDYDLAAVSTPGISD
EEEECCCCCCCEEEEEECHHHHHHCCCEECCHHHHHHHHHHHHCCCCCCEEEECCCCCCC
PSEVVSEYLSTNPKSDTGYNNPKVDELIVKGIGTTDIEKRKAIYKELYKELSDDPPVILL
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
NYRKLLYAHNARIKGIDPEKYDSISSNLPVLSIEQ
CCHHHHHHCCCEEECCCCHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]