Click here to switch to the map view.

The map label for this gene is gltX [H]

Identifier: 218901290

GI number: 218901290

Start: 97317

End: 98792

Strand: Direct

Name: gltX [H]

Synonym: BCAH820_0098

Alternate gene names: 218901290

Gene position: 97317-98792 (Clockwise)

Preceding gene: 218901289

Following gene: 218901291

Centisome position: 1.84

GC content: 36.99

Gene sequence:

>1476_bases
ATGGAAGGTGTACCAATTATGGAAAAGCAAGTGAGAGTGCGCTATGCGCCAAGTCCAACAGGACACTTACATATCGGAAA
TGCGCGTACGGCATTATTTAATTATTTATTTGCTCGTCATCAAGATGGTAAGTTTATTATTCGTATTGAAGATACTGATG
TAAAACGTAATGTTGCTGGTGGAGAAGAAAGCCAATTAAAATACTTGAAATGGCTCGGTATGGACTGGGATGAAGGTGTT
GATGTTGGTGGTGAATTTGGACCATATCGTCAAACAGAGCGTTTAGATATTTATAAAAAGTTATATGAAGATTTATTAGA
GCGTGGTTTAGCTTACAAATGTTATATGACAGAAGAAGAGCTAGAAGCAGAACGCGAAGGGCAAATTGCTCGTGGTGAAA
CACCTCGTTACGCAGGTAACCACCGTGATTTAACTGAAGCGCAAGTGAAAGAATTTGAAGCTGAGGGACGTATTCCAAGT
ATTCGTTTCCGCGTACCAGCTGACCGTGATTACACATTTAAAGATATTGTAAAAGATGAAGTTGCATTCCATTCAAATGA
TTTCGGTGATTTCGTTATCGTGAAAAAAGATGGGATTCCAACTTATAACTTTGCAGTAGCAGTAGATGATCACTTAATGG
AAATTACACACGTACTTCGTGGTGATGACCATATTTCAAACACGCCAAAACAAATGATGATTTATGAAGCTTTCGGTTGG
GATATTCCGCAATTCGGTCATATGACTTTAATTGTAAATGAAAGCCGTAAAAAATTAAGTAAGCGTGATGAATCTATTAT
TCAATTTATTGAGCAATATAAAGAGCTTGGATATCTTCCAGAAGCAATCTTTAACTTTATTGCACTACTAGGTTGGTCGC
CAGTAGGAGAAGAAGAAATTTTCTCTCAAGAAGAGTTTATCAAAATGTTTGATGCAGCTCGTTTATCGAAATCACCTGCA
TTATTTGATTCTCAAAAACTAAAATGGATGAACAACCAATATATGAAAAAGCAAGATTTAGATACGGTGGTAGAATTAAG
CTTACCGCATTTAGTGAAGGCTGGACGTATAGGTGAAACTTTAAGTGAACAAGAACAAGCTTGGATTCGTGATGTAATTG
CGTTATATCATGAACAAATGAGCTTTGGAGCTGAAATTGTAGAGCTTTCTGAAATGTTTTTCAAAGATCACGTTGATTAT
GAAGAAGAAGGACAAGAAGTATTAAAAGGTGAACAAGTACCGGAAGTACTTCGTGCATTTGCTGGTCAAGTAGAAGCACT
AGAAGCTATGGAACCGGCAGCAATTAAGACGGCTATTAAAGCGGTTCAAAAGGAAACAGGTCATAAAGGTAAAAACTTAT
TTATGCCAATCCGTGTTGCAACTACTGGTCAAACACATGGCCCAGAGCTTCCTAATGCTATTGCACTTCTTGGAAAAGAA
AAAGTTTTAAATCGTCTTCAAAAAGTAATTGGTTAA

Upstream 100 bases:

>100_bases
AAGCAGTTGTTTTATTACAGAAAAAATAATGGTTTTTAATTCGTAAGATGGATAATCACATTTTTTTGGTGTACAATTAT
AGAATGTGTTGACATTAAGA

Downstream 100 bases:

>100_bases
CATTTTCTAGGTTTAGAAATATAATAAGTATACTTAAAACCTAATAAGGAAAAGCGACGAGAAGGAGAAGTAGAAAATCA
GGCTTTTTACAGAGAGAACC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MEGVPIMEKQVRVRYAPSPTGHLHIGNARTALFNYLFARHQDGKFIIRIEDTDVKRNVAGGEESQLKYLKWLGMDWDEGV
DVGGEFGPYRQTERLDIYKKLYEDLLERGLAYKCYMTEEELEAEREGQIARGETPRYAGNHRDLTEAQVKEFEAEGRIPS
IRFRVPADRDYTFKDIVKDEVAFHSNDFGDFVIVKKDGIPTYNFAVAVDDHLMEITHVLRGDDHISNTPKQMMIYEAFGW
DIPQFGHMTLIVNESRKKLSKRDESIIQFIEQYKELGYLPEAIFNFIALLGWSPVGEEEIFSQEEFIKMFDAARLSKSPA
LFDSQKLKWMNNQYMKKQDLDTVVELSLPHLVKAGRIGETLSEQEQAWIRDVIALYHEQMSFGAEIVELSEMFFKDHVDY
EEEGQEVLKGEQVPEVLRAFAGQVEALEAMEPAAIKTAIKAVQKETGHKGKNLFMPIRVATTGQTHGPELPNAIALLGKE
KVLNRLQKVIG

Sequences:

>Translated_491_residues
MEGVPIMEKQVRVRYAPSPTGHLHIGNARTALFNYLFARHQDGKFIIRIEDTDVKRNVAGGEESQLKYLKWLGMDWDEGV
DVGGEFGPYRQTERLDIYKKLYEDLLERGLAYKCYMTEEELEAEREGQIARGETPRYAGNHRDLTEAQVKEFEAEGRIPS
IRFRVPADRDYTFKDIVKDEVAFHSNDFGDFVIVKKDGIPTYNFAVAVDDHLMEITHVLRGDDHISNTPKQMMIYEAFGW
DIPQFGHMTLIVNESRKKLSKRDESIIQFIEQYKELGYLPEAIFNFIALLGWSPVGEEEIFSQEEFIKMFDAARLSKSPA
LFDSQKLKWMNNQYMKKQDLDTVVELSLPHLVKAGRIGETLSEQEQAWIRDVIALYHEQMSFGAEIVELSEMFFKDHVDY
EEEGQEVLKGEQVPEVLRAFAGQVEALEAMEPAAIKTAIKAVQKETGHKGKNLFMPIRVATTGQTHGPELPNAIALLGKE
KVLNRLQKVIG
>Mature_491_residues
MEGVPIMEKQVRVRYAPSPTGHLHIGNARTALFNYLFARHQDGKFIIRIEDTDVKRNVAGGEESQLKYLKWLGMDWDEGV
DVGGEFGPYRQTERLDIYKKLYEDLLERGLAYKCYMTEEELEAEREGQIARGETPRYAGNHRDLTEAQVKEFEAEGRIPS
IRFRVPADRDYTFKDIVKDEVAFHSNDFGDFVIVKKDGIPTYNFAVAVDDHLMEITHVLRGDDHISNTPKQMMIYEAFGW
DIPQFGHMTLIVNESRKKLSKRDESIIQFIEQYKELGYLPEAIFNFIALLGWSPVGEEEIFSQEEFIKMFDAARLSKSPA
LFDSQKLKWMNNQYMKKQDLDTVVELSLPHLVKAGRIGETLSEQEQAWIRDVIALYHEQMSFGAEIVELSEMFFKDHVDY
EEEGQEVLKGEQVPEVLRAFAGQVEALEAMEPAAIKTAIKAVQKETGHKGKNLFMPIRVATTGQTHGPELPNAIALLGKE
KVLNRLQKVIG

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=335, Percent_Identity=39.4029850746269, Blast_Score=248, Evalue=9e-66,
Organism=Homo sapiens, GI4826960, Length=324, Percent_Identity=24.3827160493827, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1788743, Length=481, Percent_Identity=35.5509355509356, Blast_Score=321, Evalue=8e-89,
Organism=Escherichia coli, GI226510925, Length=255, Percent_Identity=33.7254901960784, Blast_Score=135, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI71993394, Length=500, Percent_Identity=32.4, Blast_Score=242, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6324540, Length=505, Percent_Identity=33.8613861386139, Blast_Score=252, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6324742, Length=314, Percent_Identity=26.4331210191083, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321192, Length=220, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24665321, Length=492, Percent_Identity=33.739837398374, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI17864488, Length=310, Percent_Identity=24.8387096774194, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 56274; Mature: 56274

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGVPIMEKQVRVRYAPSPTGHLHIGNARTALFNYLFARHQDGKFIIRIEDTDVKRNVAG
CCCCCCCCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCHHCCCC
GEESQLKYLKWLGMDWDEGVDVGGEFGPYRQTERLDIYKKLYEDLLERGLAYKCYMTEEE
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHHH
LEAEREGQIARGETPRYAGNHRDLTEAQVKEFEAEGRIPSIRFRVPADRDYTFKDIVKDE
HHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHH
VAFHSNDFGDFVIVKKDGIPTYNFAVAVDDHLMEITHVLRGDDHISNTPKQMMIYEAFGW
HHHCCCCCCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCHHEEEEHHHCC
DIPQFGHMTLIVNESRKKLSKRDESIIQFIEQYKELGYLPEAIFNFIALLGWSPVGEEEI
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHH
FSQEEFIKMFDAARLSKSPALFDSQKLKWMNNQYMKKQDLDTVVELSLPHLVKAGRIGET
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
LSEQEQAWIRDVIALYHEQMSFGAEIVELSEMFFKDHVDYEEEGQEVLKGEQVPEVLRAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHH
AGQVEALEAMEPAAIKTAIKAVQKETGHKGKNLFMPIRVATTGQTHGPELPNAIALLGKE
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHH
KVLNRLQKVIG
HHHHHHHHHHC
>Mature Secondary Structure
MEGVPIMEKQVRVRYAPSPTGHLHIGNARTALFNYLFARHQDGKFIIRIEDTDVKRNVAG
CCCCCCCCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCHHCCCC
GEESQLKYLKWLGMDWDEGVDVGGEFGPYRQTERLDIYKKLYEDLLERGLAYKCYMTEEE
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHHH
LEAEREGQIARGETPRYAGNHRDLTEAQVKEFEAEGRIPSIRFRVPADRDYTFKDIVKDE
HHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHH
VAFHSNDFGDFVIVKKDGIPTYNFAVAVDDHLMEITHVLRGDDHISNTPKQMMIYEAFGW
HHHCCCCCCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCHHEEEEHHHCC
DIPQFGHMTLIVNESRKKLSKRDESIIQFIEQYKELGYLPEAIFNFIALLGWSPVGEEEI
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHH
FSQEEFIKMFDAARLSKSPALFDSQKLKWMNNQYMKKQDLDTVVELSLPHLVKAGRIGET
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
LSEQEQAWIRDVIALYHEQMSFGAEIVELSEMFFKDHVDYEEEGQEVLKGEQVPEVLRAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHH
AGQVEALEAMEPAAIKTAIKAVQKETGHKGKNLFMPIRVATTGQTHGPELPNAIALLGKE
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHH
KVLNRLQKVIG
HHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA