Click here to switch to the map view.

The map label for this gene is spoVC [H]

Identifier: 218901253

GI number: 218901253

Start: 53509

End: 54132

Strand: Direct

Name: spoVC [H]

Synonym: BCAH820_0057

Alternate gene names: 218901253

Gene position: 53509-54132 (Clockwise)

Preceding gene: 218901252

Following gene: 218901254

Centisome position: 1.01

GC content: 34.29

Gene sequence:

>624_bases
ATGAGACGTAACCAAGATTGGTTACGTCTTTTCGTATCGAAAAAAGAAAGTAGTGGTACAAGAATGAAATTGATAGTAGG
ACTTGGGAACCCGGGTAGAGAATATGAATTAACAAGGCATAATATTGGATTTATGGCAATTGATGAACTTGCAAAGCGTT
GGAATATTTCTTTGAACGAACAAAAGTTTAAAGGAGTATTTGGTGCAGGATTTGTTAATGGAGAAAAAGTAATCTTATTA
AAGCCACTTACATATATGAATTTATCTGGGGAAAGTATTCGTCCGCTAATGGATTACTATAAAATTGATGTAGAGGATTT
CGTTGTTCTGTACGATGATTTAGATATCCCTGTAGGTAAATTACGCCTTCGTATGAAAGGTAGTGCTGGTGGACATAATG
GTGTGAAATCAACAATTTCACATTTAGGAACACAAGAGTTTCAACGTATCCGCATGGGAATTGATCGTCCGAAAAATGGA
ATGAAGGTAGTAGATTATGTATTAGGACGTTTTACATCGGAAGAAATTCCTGATGTAAATCATTCTATTGAAAAAGCAGC
AGATGCATGTGAAGAATGGTTAAATAAACCTTTTCTTCAAATCATGAATACTTTCAATAGTTAA

Upstream 100 bases:

>100_bases
CAAAGTACATGAACTTTCAGTTGCTCCACTAATCGGAGAAGCAATCATTCGTGTATACGAAGAAGAATCTGTAAGTGTAT
TATTCAATTAATTGGATAGA

Downstream 100 bases:

>100_bases
GGGTATGATTAGGAATATTTTATTCTGTTTTTACCCATACTAGCAGTAATTGTATTTTTAGGAGGCTAGTATGGAAGGGT
ATTATTATTGCAGACATTGC

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MRRNQDWLRLFVSKKESSGTRMKLIVGLGNPGREYELTRHNIGFMAIDELAKRWNISLNEQKFKGVFGAGFVNGEKVILL
KPLTYMNLSGESIRPLMDYYKIDVEDFVVLYDDLDIPVGKLRLRMKGSAGGHNGVKSTISHLGTQEFQRIRMGIDRPKNG
MKVVDYVLGRFTSEEIPDVNHSIEKAADACEEWLNKPFLQIMNTFNS

Sequences:

>Translated_207_residues
MRRNQDWLRLFVSKKESSGTRMKLIVGLGNPGREYELTRHNIGFMAIDELAKRWNISLNEQKFKGVFGAGFVNGEKVILL
KPLTYMNLSGESIRPLMDYYKIDVEDFVVLYDDLDIPVGKLRLRMKGSAGGHNGVKSTISHLGTQEFQRIRMGIDRPKNG
MKVVDYVLGRFTSEEIPDVNHSIEKAADACEEWLNKPFLQIMNTFNS
>Mature_207_residues
MRRNQDWLRLFVSKKESSGTRMKLIVGLGNPGREYELTRHNIGFMAIDELAKRWNISLNEQKFKGVFGAGFVNGEKVILL
KPLTYMNLSGESIRPLMDYYKIDVEDFVVLYDDLDIPVGKLRLRMKGSAGGHNGVKSTISHLGTQEFQRIRMGIDRPKNG
MKVVDYVLGRFTSEEIPDVNHSIEKAADACEEWLNKPFLQIMNTFNS

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis [H]

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family [H]

Homologues:

Organism=Homo sapiens, GI50897284, Length=168, Percent_Identity=32.1428571428571, Blast_Score=104, Evalue=7e-23,
Organism=Escherichia coli, GI1787455, Length=188, Percent_Identity=39.3617021276596, Blast_Score=122, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321983, Length=182, Percent_Identity=30.2197802197802, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001328
- InterPro:   IPR018171 [H]

Pfam domain/function: PF01195 Pept_tRNA_hydro [H]

EC number: =3.1.1.29 [H]

Molecular weight: Translated: 23660; Mature: 23660

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1 ; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRNQDWLRLFVSKKESSGTRMKLIVGLGNPGREYELTRHNIGFMAIDELAKRWNISLNE
CCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEHHCCCCHHHHHHHHHHHCCCCCH
QKFKGVFGAGFVNGEKVILLKPLTYMNLSGESIRPLMDYYKIDVEDFVVLYDDLDIPVGK
HHHCCCCCCCEECCCEEEEECCCEEECCCCCCHHHHHHHHHCCHHHEEEEEECCCCCCCC
LRLRMKGSAGGHNGVKSTISHLGTQEFQRIRMGIDRPKNGMKVVDYVLGRFTSEEIPDVN
EEEEEECCCCCCCHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCH
HSIEKAADACEEWLNKPFLQIMNTFNS
HHHHHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MRRNQDWLRLFVSKKESSGTRMKLIVGLGNPGREYELTRHNIGFMAIDELAKRWNISLNE
CCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEHHCCCCHHHHHHHHHHHCCCCCH
QKFKGVFGAGFVNGEKVILLKPLTYMNLSGESIRPLMDYYKIDVEDFVVLYDDLDIPVGK
HHHCCCCCCCEECCCEEEEECCCEEECCCCCCHHHHHHHHHCCHHHEEEEEECCCCCCCC
LRLRMKGSAGGHNGVKSTISHLGTQEFQRIRMGIDRPKNGMKVVDYVLGRFTSEEIPDVN
EEEEEECCCCCCCHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCH
HSIEKAADACEEWLNKPFLQIMNTFNS
HHHHHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]