Click here to switch to the map view.

The map label for this gene is tpiA [H]

Identifier: 218884557

GI number: 218884557

Start: 1167200

End: 1167895

Strand: Reverse

Name: tpiA [H]

Synonym: DKAM_1246

Alternate gene names: 218884557

Gene position: 1167895-1167200 (Counterclockwise)

Preceding gene: 218884558

Following gene: 218884556

Centisome position: 85.55

GC content: 48.56

Gene sequence:

>696_bases
ATGGTTAAGCCAGTGCTAGCTGTGAATTTTAAGGCCTATTACCCCCATAGTTTTGGTGAAAACGCTTATAGATTAGCAAA
AGACGCTGTCAGGGTGTGGAGAGAGACCGGTGTAGAAGTAATACTTGCACCACCATTCACCGAGTTGAAAAGCGTTATCG
AAGCCGTGAAAGATACCGGGGTAAAGGTATTCGCCCAGCATGCTGACCCAGTGGAGCCCGGGGCTATTACAGGGTTTATA
CCATTAGAGGGGTTGAAGAACCTGGGGGCACATGGTGTAATACTGAACCATAGTGAGCATAGGCTAAAAATCTCTGATAT
AAACTATTTGGTTAAGAAGGCAAGGAGACTCGGCATCGAGACACTTGTATGTGCGGATACGCCTGAAACAGGTGCTGCTG
TAGCAGTACTGGGACCGGACATGGTTGCCGTAGAACCGCCCGAGCTAATTGGAACAGGTGTAAGCGTAAGCAAGGCTAAG
CCGGAGGTAGTGACTAACAGTGTAGACATGATTAGACGGGTCAATAAAGACGTAGCCATACTAACGGGTGCTGGGATAAC
GACTGGCGAGGACGCCTACATAGCTGTGAAACTCGGCACAATAGGGGTCTTAGTAGCCTCCGGGATAGTGAAAGCAAAAG
ACCCCTATCAAGTAATGAAAGAAATGGCTGAAGCCATGCTCAAGGGCCTCGCCTAA

Upstream 100 bases:

>100_bases
ATAGATTTGTAAAAAGTGAGGAAGAAGCCAAGGTAGTGAAGAAGCATAGTGATATGCTATCCGGAAGCTAAGCTCATAGC
CGAGACATGGTGGATGAGAC

Downstream 100 bases:

>100_bases
ACCGTAAACCTCTACATGTCTATGTTTTTAACTGCTTACCTGAACCATGCCTTCATATATTAAACCGTAGCGGGCTGTGT
CTGGCACGGCTCGGTTCATC

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MVKPVLAVNFKAYYPHSFGENAYRLAKDAVRVWRETGVEVILAPPFTELKSVIEAVKDTGVKVFAQHADPVEPGAITGFI
PLEGLKNLGAHGVILNHSEHRLKISDINYLVKKARRLGIETLVCADTPETGAAVAVLGPDMVAVEPPELIGTGVSVSKAK
PEVVTNSVDMIRRVNKDVAILTGAGITTGEDAYIAVKLGTIGVLVASGIVKAKDPYQVMKEMAEAMLKGLA

Sequences:

>Translated_231_residues
MVKPVLAVNFKAYYPHSFGENAYRLAKDAVRVWRETGVEVILAPPFTELKSVIEAVKDTGVKVFAQHADPVEPGAITGFI
PLEGLKNLGAHGVILNHSEHRLKISDINYLVKKARRLGIETLVCADTPETGAAVAVLGPDMVAVEPPELIGTGVSVSKAK
PEVVTNSVDMIRRVNKDVAILTGAGITTGEDAYIAVKLGTIGVLVASGIVKAKDPYQVMKEMAEAMLKGLA
>Mature_231_residues
MVKPVLAVNFKAYYPHSFGENAYRLAKDAVRVWRETGVEVILAPPFTELKSVIEAVKDTGVKVFAQHADPVEPGAITGFI
PLEGLKNLGAHGVILNHSEHRLKISDINYLVKKARRLGIETLVCADTPETGAAVAVLGPDMVAVEPPELIGTGVSVSKAK
PEVVTNSVDMIRRVNKDVAILTGAGITTGEDAYIAVKLGTIGVLVASGIVKAKDPYQVMKEMAEAMLKGLA

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

None

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000652
- InterPro:   IPR022891
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 24578; Mature: 24578

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKPVLAVNFKAYYPHSFGENAYRLAKDAVRVWRETGVEVILAPPFTELKSVIEAVKDTG
CCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCC
VKVFAQHADPVEPGAITGFIPLEGLKNLGAHGVILNHSEHRLKISDINYLVKKARRLGIE
EEEEEECCCCCCCCCEEEEEEHHHHHCCCCCEEEEECCCCEEEEHHHHHHHHHHHHCCCE
TLVCADTPETGAAVAVLGPDMVAVEPPELIGTGVSVSKAKPEVVTNSVDMIRRVNKDVAI
EEEECCCCCCCCEEEEECCCEEEECCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
LTGAGITTGEDAYIAVKLGTIGVLVASGIVKAKDPYQVMKEMAEAMLKGLA
EECCCCCCCCCCEEEEEECHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVKPVLAVNFKAYYPHSFGENAYRLAKDAVRVWRETGVEVILAPPFTELKSVIEAVKDTG
CCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCC
VKVFAQHADPVEPGAITGFIPLEGLKNLGAHGVILNHSEHRLKISDINYLVKKARRLGIE
EEEEEECCCCCCCCCEEEEEEHHHHHCCCCCEEEEECCCCEEEEHHHHHHHHHHHHCCCE
TLVCADTPETGAAVAVLGPDMVAVEPPELIGTGVSVSKAKPEVVTNSVDMIRRVNKDVAI
EEEECCCCCCCCEEEEECCCEEEECCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
LTGAGITTGEDAYIAVKLGTIGVLVASGIVKAKDPYQVMKEMAEAMLKGLA
EECCCCCCCCCCEEEEEECHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]