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The map label for this gene is radA [H]

Identifier: 218884526

GI number: 218884526

Start: 1136697

End: 1137683

Strand: Reverse

Name: radA [H]

Synonym: DKAM_1215

Alternate gene names: 218884526

Gene position: 1137683-1136697 (Counterclockwise)

Preceding gene: 218884528

Following gene: 218884523

Centisome position: 83.33

GC content: 47.92

Gene sequence:

>987_bases
ATGAGTGAGGAGAAGGAAACCATAAAGGAGCGTTCCTCGGGCTTCATCTCTGTGAGGGATATACCTGGCGTAGGATCCAG
TATAGCTGATAAACTAGAGGCAGCTGGATACTTATCGGCCTGGAGCATTGTTGTTGCACGCGCAGAGGAGTTGGCTGAGA
GAACCGGACTCCCAGTTCTCACTGTTCAGAAGATCATTGAGAATGCACGGAAGATGTTGGGTATAACGTTTAAGACCGCT
AGGGAGGTTAAACAAGAGAGATTGAATATCGGGAAGATCACGACGGGGAGCAAGAGCCTTGATGAACTACTTGGTGGGGG
AGTCGAGACGAAAACCATCACGGAGTTCTTTGGTGAATATGGATCCGGGAAGACACAGATTTGCCACCAATTGAGTGTAA
ATGTCCAATTAACCCCTGAGAAAGGCGGCCTCAATGGTAGGGCTGTCTACATTGATACAGAGGGCACATTCCGCTGGGAG
AGGATAGAGGCGATGGCAAGGGCCCTCGGCTTAGACCCCGATAAAGTCATGGATAATATATATTATATGAGAGCATACAA
CAGCGATCACCAGATAGCTATAGTGGACGAATTATTTACATTCGTACCGAAGAATGATGTCAGGCTAGTAATCCTTGACA
GTGTCACAAGCCACTTCAGGGCTGAGTACCCTGGTAGAGAACATTTGGCCGAACGCCAGCAGAAGTTAAACTCCCATCTA
CACCAGTTAATGAGGCTGGCTGAAGCATACAATGTTGCTGTAGTTGTAACAAACCAGGTAATGGCTAGGCCAGATGTATT
CTACGGTGACCCGACAACGGCTGTAGGAGGACATGTATTGGCGCATACACCGGGGGTGAGGATTCAGCTAAGGAAGTCTA
AGGGCAATAAGAGAATTGCAAGAGTAGTTGATGCTCCTCACTTACCTGAGGGAGAAGTAGTATTCGTGATAACTGAGGAG
GGTATCAGGGACTCCGAGGAAGAGTAA

Upstream 100 bases:

>100_bases
ATATGTATTGATATATTGATTTACCATATACTAAAGGTTACGTGACCACGTTATCCTATATTAGGCTAGAAAACAATAAT
TTAGAAAAGGTGCTGGATTC

Downstream 100 bases:

>100_bases
GAGTAGGTGCGATCTGTAATCTAACACATACTGTCTTTTAACAATCATTGCTGGATGAATCATACCTGGGTTGACTATGC
TACAGGATCAGCTCGAGTAA

Product: DNA repair and recombination protein RadA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSEEKETIKERSSGFISVRDIPGVGSSIADKLEAAGYLSAWSIVVARAEELAERTGLPVLTVQKIIENARKMLGITFKTA
REVKQERLNIGKITTGSKSLDELLGGGVETKTITEFFGEYGSGKTQICHQLSVNVQLTPEKGGLNGRAVYIDTEGTFRWE
RIEAMARALGLDPDKVMDNIYYMRAYNSDHQIAIVDELFTFVPKNDVRLVILDSVTSHFRAEYPGREHLAERQQKLNSHL
HQLMRLAEAYNVAVVVTNQVMARPDVFYGDPTTAVGGHVLAHTPGVRIQLRKSKGNKRIARVVDAPHLPEGEVVFVITEE
GIRDSEEE

Sequences:

>Translated_328_residues
MSEEKETIKERSSGFISVRDIPGVGSSIADKLEAAGYLSAWSIVVARAEELAERTGLPVLTVQKIIENARKMLGITFKTA
REVKQERLNIGKITTGSKSLDELLGGGVETKTITEFFGEYGSGKTQICHQLSVNVQLTPEKGGLNGRAVYIDTEGTFRWE
RIEAMARALGLDPDKVMDNIYYMRAYNSDHQIAIVDELFTFVPKNDVRLVILDSVTSHFRAEYPGREHLAERQQKLNSHL
HQLMRLAEAYNVAVVVTNQVMARPDVFYGDPTTAVGGHVLAHTPGVRIQLRKSKGNKRIARVVDAPHLPEGEVVFVITEE
GIRDSEEE
>Mature_327_residues
SEEKETIKERSSGFISVRDIPGVGSSIADKLEAAGYLSAWSIVVARAEELAERTGLPVLTVQKIIENARKMLGITFKTAR
EVKQERLNIGKITTGSKSLDELLGGGVETKTITEFFGEYGSGKTQICHQLSVNVQLTPEKGGLNGRAVYIDTEGTFRWER
IEAMARALGLDPDKVMDNIYYMRAYNSDHQIAIVDELFTFVPKNDVRLVILDSVTSHFRAEYPGREHLAERQQKLNSHLH
QLMRLAEAYNVAVVVTNQVMARPDVFYGDPTTAVGGHVLAHTPGVRIQLRKSKGNKRIARVVDAPHLPEGEVVFVITEEG
IRDSEEE

Specific function: Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules [H]

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic recA-like protein family [H]

Homologues:

Organism=Homo sapiens, GI19924133, Length=315, Percent_Identity=42.8571428571429, Blast_Score=265, Evalue=3e-71,
Organism=Homo sapiens, GI23238219, Length=334, Percent_Identity=41.0179640718563, Blast_Score=244, Evalue=7e-65,
Organism=Homo sapiens, GI256017143, Length=317, Percent_Identity=38.1703470031546, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI256017141, Length=317, Percent_Identity=38.1703470031546, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI256017145, Length=233, Percent_Identity=43.3476394849785, Blast_Score=191, Evalue=8e-49,
Organism=Homo sapiens, GI17402896, Length=329, Percent_Identity=29.7872340425532, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI19924117, Length=333, Percent_Identity=26.7267267267267, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI10835029, Length=333, Percent_Identity=26.7267267267267, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI46255039, Length=333, Percent_Identity=26.7267267267267, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI153946430, Length=282, Percent_Identity=30.1418439716312, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI4885659, Length=282, Percent_Identity=30.1418439716312, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI153946427, Length=282, Percent_Identity=30.1418439716312, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1789051, Length=210, Percent_Identity=26.1904761904762, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71997301, Length=302, Percent_Identity=44.0397350993377, Blast_Score=259, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI71997295, Length=302, Percent_Identity=44.0397350993377, Blast_Score=258, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6321027, Length=309, Percent_Identity=44.0129449838188, Blast_Score=264, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6320942, Length=331, Percent_Identity=40.4833836858006, Blast_Score=240, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6320207, Length=183, Percent_Identity=36.0655737704918, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI17864108, Length=310, Percent_Identity=41.6129032258065, Blast_Score=240, Evalue=9e-64,
Organism=Drosophila melanogaster, GI24651285, Length=268, Percent_Identity=44.0298507462687, Blast_Score=234, Evalue=5e-62,
Organism=Drosophila melanogaster, GI17136506, Length=277, Percent_Identity=28.5198555956679, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24650551, Length=197, Percent_Identity=32.48730964467, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013632
- InterPro:   IPR011938
- InterPro:   IPR016467
- InterPro:   IPR020588
- InterPro:   IPR010995
- InterPro:   IPR020587 [H]

Pfam domain/function: PF08423 Rad51 [H]

EC number: NA

Molecular weight: Translated: 36461; Mature: 36330

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS50162 RECA_2 ; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEKETIKERSSGFISVRDIPGVGSSIADKLEAAGYLSAWSIVVARAEELAERTGLPVL
CCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHH
TVQKIIENARKMLGITFKTAREVKQERLNIGKITTGSKSLDELLGGGVETKTITEFFGEY
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHH
GSGKTQICHQLSVNVQLTPEKGGLNGRAVYIDTEGTFRWERIEAMARALGLDPDKVMDNI
CCCHHHHHHEEEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHE
YYMRAYNSDHQIAIVDELFTFVPKNDVRLVILDSVTSHFRAEYPGREHLAERQQKLNSHL
EEEEEECCCCCEEEHHHHHHHCCCCCCEEEEEECHHHHHHCCCCCHHHHHHHHHHHHHHH
HQLMRLAEAYNVAVVVTNQVMARPDVFYGDPTTAVGGHVLAHTPGVRIQLRKSKGNKRIA
HHHHHHHHHCCEEEEEECHHHCCCCEEECCCCCHHCCEEEEECCCCEEEEECCCCCHHHH
RVVDAPHLPEGEVVFVITEEGIRDSEEE
HHHCCCCCCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure 
SEEKETIKERSSGFISVRDIPGVGSSIADKLEAAGYLSAWSIVVARAEELAERTGLPVL
CCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHH
TVQKIIENARKMLGITFKTAREVKQERLNIGKITTGSKSLDELLGGGVETKTITEFFGEY
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHH
GSGKTQICHQLSVNVQLTPEKGGLNGRAVYIDTEGTFRWERIEAMARALGLDPDKVMDNI
CCCHHHHHHEEEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHE
YYMRAYNSDHQIAIVDELFTFVPKNDVRLVILDSVTSHFRAEYPGREHLAERQQKLNSHL
EEEEEECCCCCEEEHHHHHHHCCCCCCEEEEEECHHHHHHCCCCCHHHHHHHHHHHHHHH
HQLMRLAEAYNVAVVVTNQVMARPDVFYGDPTTAVGGHVLAHTPGVRIQLRKSKGNKRIA
HHHHHHHHHCCEEEEEECHHHCCCCEEECCCCCHHCCEEEEECCCCEEEEECCCCCHHHH
RVVDAPHLPEGEVVFVITEEGIRDSEEE
HHHCCCCCCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA