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The map label for this gene is ppa [H]

Identifier: 218884349

GI number: 218884349

Start: 985449

End: 985976

Strand: Reverse

Name: ppa [H]

Synonym: DKAM_1038

Alternate gene names: 218884349

Gene position: 985976-985449 (Counterclockwise)

Preceding gene: 218884350

Following gene: 218884348

Centisome position: 72.22

GC content: 43.18

Gene sequence:

>528_bases
ATGAGCCAATACAGTAAGCTCGGTCCTGGTTCTAAAGCCCCTGAGGAAGTATATGTGGTTATAGAGATCCCGGTTAACAG
TGGAGTCAAGTATGAACTGGATAAGGAGACAGGGGTTTTAATGGTTGATAGAATACTGTATACTTCAATGGTATATCCAT
TCAACTACGGTTTCATACCCGGTACGTTGGAAGAAGATGGAGACCCGGTTGATGTCCTGGTTGTATCAAGCGAGCCTCTA
ATACCTGGCTCAGTTATAAAGGTAAGGCCTATAGGAGTCTTAGAAACAGAGGATGAAAAGGGAAGGGATGCGAAGATTTT
GGCTGTCCCAGTTGATAAAGTAGACCCGAGGTATAGTAATATAAGAGATGTAGGAGACCTACCTGAGGCAGTTAAGCAGA
GGATAAAGCATTTCTTCGAGCACTATAAGGAGCTGGAACCTGGTAAATGGGTTAAAGTAGTAGGGTGGAAAGGAAGAGAG
GAAGCCTTAAAGAGAATTAATGACGCAATTAATAGGGCCAAAGGCTAA

Upstream 100 bases:

>100_bases
AATACTGCTGAAACTATGAGCTTAACTAATAGAGAATACTTTTTATTCTCTACGGTATTTAATCCCTAGAATAGTGTCCG
CAATTGGGGGTGCAGGAGTC

Downstream 100 bases:

>100_bases
CATAGTGTTTTTCACGATTAGAAACCATACCCCTTTCCGTTTCCCGCCTCGGTATGTTGTTTCCGCTTTCATATAGGTTT
ACTACTGAATTCTTTTATTG

Product: inorganic pyrophosphatase

Products: NA

Alternate protein names: Pyrophosphate phospho-hydrolase; PPase [H]

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MSQYSKLGPGSKAPEEVYVVIEIPVNSGVKYELDKETGVLMVDRILYTSMVYPFNYGFIPGTLEEDGDPVDVLVVSSEPL
IPGSVIKVRPIGVLETEDEKGRDAKILAVPVDKVDPRYSNIRDVGDLPEAVKQRIKHFFEHYKELEPGKWVKVVGWKGRE
EALKRINDAINRAKG

Sequences:

>Translated_175_residues
MSQYSKLGPGSKAPEEVYVVIEIPVNSGVKYELDKETGVLMVDRILYTSMVYPFNYGFIPGTLEEDGDPVDVLVVSSEPL
IPGSVIKVRPIGVLETEDEKGRDAKILAVPVDKVDPRYSNIRDVGDLPEAVKQRIKHFFEHYKELEPGKWVKVVGWKGRE
EALKRINDAINRAKG
>Mature_174_residues
SQYSKLGPGSKAPEEVYVVIEIPVNSGVKYELDKETGVLMVDRILYTSMVYPFNYGFIPGTLEEDGDPVDVLVVSSEPLI
PGSVIKVRPIGVLETEDEKGRDAKILAVPVDKVDPRYSNIRDVGDLPEAVKQRIKHFFEHYKELEPGKWVKVVGWKGREE
ALKRINDAINRAKG

Specific function: Unknown

COG id: COG0221

COG function: function code C; Inorganic pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790673, Length=167, Percent_Identity=57.4850299401198, Blast_Score=194, Evalue=2e-51,

Paralogues:

None

Copy number: 5480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008162 [H]

Pfam domain/function: PF00719 Pyrophosphatase [H]

EC number: =3.6.1.1 [H]

Molecular weight: Translated: 19612; Mature: 19481

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQYSKLGPGSKAPEEVYVVIEIPVNSGVKYELDKETGVLMVDRILYTSMVYPFNYGFIP
CCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEECCCCCEEHHHHHHHHHHHCCCCCCCCC
GTLEEDGDPVDVLVVSSEPLIPGSVIKVRPIGVLETEDEKGRDAKILAVPVDKVDPRYSN
CCCCCCCCCEEEEEECCCCCCCCCEEEEECCEEEECCCCCCCCCEEEEEECCCCCCCHHC
IRDVGDLPEAVKQRIKHFFEHYKELEPGKWVKVVGWKGREEALKRINDAINRAKG
HHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQYSKLGPGSKAPEEVYVVIEIPVNSGVKYELDKETGVLMVDRILYTSMVYPFNYGFIP
CCCCCCCCCCCCCCEEEEEEEEECCCCCEEEECCCCCEEHHHHHHHHHHHCCCCCCCCC
GTLEEDGDPVDVLVVSSEPLIPGSVIKVRPIGVLETEDEKGRDAKILAVPVDKVDPRYSN
CCCCCCCCCEEEEEECCCCCCCCCEEEEECCEEEECCCCCCCCCEEEEEECCCCCCCHHC
IRDVGDLPEAVKQRIKHFFEHYKELEPGKWVKVVGWKGREEALKRINDAINRAKG
HHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]