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The map label for this gene is topA [H]

Identifier: 218884347

GI number: 218884347

Start: 983080

End: 985119

Strand: Direct

Name: topA [H]

Synonym: DKAM_1036

Alternate gene names: 218884347

Gene position: 983080-985119 (Clockwise)

Preceding gene: 218884346

Following gene: 218884353

Centisome position: 72.01

GC content: 44.02

Gene sequence:

>2040_bases
ATGGTGAGCAAGAGTATAAGGATATGGGATTTAAAGGGTAGGATACTCGTTATAGCTGAGAAACCTAAGGCAGCCAAGAA
AATAGCCAGTGCCCTCTCCCCTACTCATAACGCGAAAAGAATTGGCTCCATTACCATCTACGAGGTTGACTCGTATAGTT
CAAGCATAATTATTGCTAGCTCGGTGGGGCACCTCTACCAGCTGCACACAAGTCTAAGGGGGTACCCTGTATACAGCTAT
GAATGGATCCCGCTACATCTGACCGATCCAAGCAAGAAATATATAAGGGAGTACCTTGAAGTACTCGGGAAGCTTTGCCG
TGGAGTAGACTACTATGTAAACGCATGTGACTACGATATAGAGGGAAGTGTCATAGGATATCTAATAATAAAGTTCAACG
GCGACGAGAGAAAAGCCTTCAGAGCCAGGTTTTCTAGCTTAACAATAGAGGAGCTGAGAAAAGCCTTCAATAATTTAACC
AGGCTAGACTACGAGATGATAGAGGCTGGCCTATGTAGGCATGAGTTGGACTGGATATGGGGTATTAATGTAAGCCGTGC
TCTCATGAAGGCTGTCGAGGACGCTGTGGGCGAGAGGATAATCCTCAGCGCTGGCAGAGTGCAAACCCCTACACTAAAAT
ACGTTGTAGATAAAGTAGTTGAGAGAAATCTATTCATACCACTACCCCAGTATAATATAAATATTACAGTGGAGAAGAAT
GGTGAAAAAATAAGGCTAGAGTATACTGGTAACCCGGTTGAAAAGGAAAGAGATGCGAGAGACATAGTGGAGAAAATAAG
AGGATACAAGTATTTGATTGTAAGTAAAGTTGAGAAAAAACACCATATACTCCATCCACCACCCCCATTTAACCTCGGGG
ATCTACAGAAGGAGGCGGCTAGACTATACGGGTTCAGCCCAGCTGAAACCCAGTCCATAGCTGAGCAACTATACCTAGAT
GCTTTAATAAGCTATCCTAGGACGAACAGTCAGAAGCTTCCTCCAACACTAGACTACAGGAGGATACTGGAGAATATTTC
ATCGATAAAGAGTTATAGGAACCTTGTGGAAAGCCTTATATCTGAGACCAAAGGTGTGTTAACCCCCGTCGAAGGAGAGA
AGGAGGATCCAGCCCATCCAGCAATCTACCCCACTGGGGTTGTCCCAGGAAGACTCACAGAGAAGCAGTGGGCTATATAT
GATCTCATAGTAAAAAGGTTTCTAGCAGTGTTTGCCCCACCAGCTAAAATAGTTTACTACGTAGTGAAGGCTACCACACC
GTCACACGAATACGTATTCGAGACAAAGGGGCAGGTTATAGAGGAGTATGGTTGGTTGAAGTATTATAATTTCCACAACT
ACCATGCAGGCAAGATACCTGTATTAAAGGAGGGGGAGTTATTAAATATTGTAAAGGCCACGGCTAGCGAATCATATACG
AAGCCTCCTAAAAGGCTCACAAAAACAGATCTATTAAGGTGGATGGAGAGCGTTGAGATAGGTACGGAAGCTACGAGGGC
ACAGATAATTGAGAAACTCTTCGAAAGAAAATACTTATACCAACACCAAAAATCCAGCGGTATAGACGTATCCGAGATAG
GCTTTGGAGTTGTAGAGATCCTCGAGAAGTTCTTCCCGGATCTACTCAGCGTCGAACTAACACGTAAGTTCGAGAAGGAG
ATGGACAGCATAAGGAGCGGGGTTAAGAGGAGGAGCGAGGTACTGGAGGAGGCGAGAAGAGTTCTCACCGAGCTACTCGG
TAAATTTGATAGAAACAGAATTGAGGCAGGGAAACAACTGGCTTTAAGGTTGAAAATAATCCAGAACACAAGAAAGTGTC
GCGTCAGCTCATGTTATCGCGAAGGAGTCGTTAATGGACTTTGCAGCCACCACGCTAACGCAGTTAAACTAGTACGTGAA
TACTATGGCGAGTGGAGTAGAAGGAAAGACATTACTTTCAACGAGTATATTGATAAGTTAAGGAAGTCTAAGTTGACTGG
TAAATGGGTTAAGGAGGTTATAGAGTCTGAGCTAACTTAG

Upstream 100 bases:

>100_bases
GACTCAAAGTGCCCTCTATGCCTGATATTATATGTAATGCTACTGATCCTAACCATGCCTCTACCGGCCTAACAATGCCT
GTATAAGAGATAGGGGGGAG

Downstream 100 bases:

>100_bases
CTTCCATGAGTATAGCATCCACATGTCCACCTGTTCTGACCGGAAAACCGTAGGGGTTCAGGTCTTTATATATAGGTTTA
TATAAATTGGTTTCATCACT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 679; Mature: 679

Protein sequence:

>679_residues
MVSKSIRIWDLKGRILVIAEKPKAAKKIASALSPTHNAKRIGSITIYEVDSYSSSIIIASSVGHLYQLHTSLRGYPVYSY
EWIPLHLTDPSKKYIREYLEVLGKLCRGVDYYVNACDYDIEGSVIGYLIIKFNGDERKAFRARFSSLTIEELRKAFNNLT
RLDYEMIEAGLCRHELDWIWGINVSRALMKAVEDAVGERIILSAGRVQTPTLKYVVDKVVERNLFIPLPQYNINITVEKN
GEKIRLEYTGNPVEKERDARDIVEKIRGYKYLIVSKVEKKHHILHPPPPFNLGDLQKEAARLYGFSPAETQSIAEQLYLD
ALISYPRTNSQKLPPTLDYRRILENISSIKSYRNLVESLISETKGVLTPVEGEKEDPAHPAIYPTGVVPGRLTEKQWAIY
DLIVKRFLAVFAPPAKIVYYVVKATTPSHEYVFETKGQVIEEYGWLKYYNFHNYHAGKIPVLKEGELLNIVKATASESYT
KPPKRLTKTDLLRWMESVEIGTEATRAQIIEKLFERKYLYQHQKSSGIDVSEIGFGVVEILEKFFPDLLSVELTRKFEKE
MDSIRSGVKRRSEVLEEARRVLTELLGKFDRNRIEAGKQLALRLKIIQNTRKCRVSSCYREGVVNGLCSHHANAVKLVRE
YYGEWSRRKDITFNEYIDKLRKSKLTGKWVKEVIESELT

Sequences:

>Translated_679_residues
MVSKSIRIWDLKGRILVIAEKPKAAKKIASALSPTHNAKRIGSITIYEVDSYSSSIIIASSVGHLYQLHTSLRGYPVYSY
EWIPLHLTDPSKKYIREYLEVLGKLCRGVDYYVNACDYDIEGSVIGYLIIKFNGDERKAFRARFSSLTIEELRKAFNNLT
RLDYEMIEAGLCRHELDWIWGINVSRALMKAVEDAVGERIILSAGRVQTPTLKYVVDKVVERNLFIPLPQYNINITVEKN
GEKIRLEYTGNPVEKERDARDIVEKIRGYKYLIVSKVEKKHHILHPPPPFNLGDLQKEAARLYGFSPAETQSIAEQLYLD
ALISYPRTNSQKLPPTLDYRRILENISSIKSYRNLVESLISETKGVLTPVEGEKEDPAHPAIYPTGVVPGRLTEKQWAIY
DLIVKRFLAVFAPPAKIVYYVVKATTPSHEYVFETKGQVIEEYGWLKYYNFHNYHAGKIPVLKEGELLNIVKATASESYT
KPPKRLTKTDLLRWMESVEIGTEATRAQIIEKLFERKYLYQHQKSSGIDVSEIGFGVVEILEKFFPDLLSVELTRKFEKE
MDSIRSGVKRRSEVLEEARRVLTELLGKFDRNRIEAGKQLALRLKIIQNTRKCRVSSCYREGVVNGLCSHHANAVKLVRE
YYGEWSRRKDITFNEYIDKLRKSKLTGKWVKEVIESELT
>Mature_679_residues
MVSKSIRIWDLKGRILVIAEKPKAAKKIASALSPTHNAKRIGSITIYEVDSYSSSIIIASSVGHLYQLHTSLRGYPVYSY
EWIPLHLTDPSKKYIREYLEVLGKLCRGVDYYVNACDYDIEGSVIGYLIIKFNGDERKAFRARFSSLTIEELRKAFNNLT
RLDYEMIEAGLCRHELDWIWGINVSRALMKAVEDAVGERIILSAGRVQTPTLKYVVDKVVERNLFIPLPQYNINITVEKN
GEKIRLEYTGNPVEKERDARDIVEKIRGYKYLIVSKVEKKHHILHPPPPFNLGDLQKEAARLYGFSPAETQSIAEQLYLD
ALISYPRTNSQKLPPTLDYRRILENISSIKSYRNLVESLISETKGVLTPVEGEKEDPAHPAIYPTGVVPGRLTEKQWAIY
DLIVKRFLAVFAPPAKIVYYVVKATTPSHEYVFETKGQVIEEYGWLKYYNFHNYHAGKIPVLKEGELLNIVKATASESYT
KPPKRLTKTDLLRWMESVEIGTEATRAQIIEKLFERKYLYQHQKSSGIDVSEIGFGVVEILEKFFPDLLSVELTRKFEKE
MDSIRSGVKRRSEVLEEARRVLTELLGKFDRNRIEAGKQLALRLKIIQNTRKCRVSSCYREGVVNGLCSHHANAVKLVRE
YYGEWSRRKDITFNEYIDKLRKSKLTGKWVKEVIESELT

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic type I/III topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=647, Percent_Identity=27.047913446677, Blast_Score=160, Evalue=4e-39,
Organism=Homo sapiens, GI4507635, Length=609, Percent_Identity=23.9737274220033, Blast_Score=130, Evalue=5e-30,
Organism=Escherichia coli, GI1787529, Length=632, Percent_Identity=25.9493670886076, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI1788061, Length=570, Percent_Identity=26.140350877193, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17555378, Length=589, Percent_Identity=27.5042444821732, Blast_Score=184, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI32563869, Length=595, Percent_Identity=25.0420168067227, Blast_Score=135, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6323263, Length=644, Percent_Identity=23.1366459627329, Blast_Score=127, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24585251, Length=615, Percent_Identity=27.8048780487805, Blast_Score=155, Evalue=9e-38,
Organism=Drosophila melanogaster, GI24640096, Length=596, Percent_Identity=23.489932885906, Blast_Score=137, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013826
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005739
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 78158; Mature: 78158

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSKSIRIWDLKGRILVIAEKPKAAKKIASALSPTHNAKRIGSITIYEVDSYSSSIIIAS
CCCCCEEEEEECCCEEEEECCCHHHHHHHHHHCCCCCHHHCCCEEEEEECCCCCCEEEEE
SVGHLYQLHTSLRGYPVYSYEWIPLHLTDPSKKYIREYLEVLGKLCRGVDYYVNACDYDI
HHHHHHHHHHHCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCC
EGSVIGYLIIKFNGDERKAFRARFSSLTIEELRKAFNNLTRLDYEMIEAGLCRHELDWIW
CCCEEEEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
GINVSRALMKAVEDAVGERIILSAGRVQTPTLKYVVDKVVERNLFIPLPQYNINITVEKN
CCCHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCEEECCCCEEEEEEECC
GEKIRLEYTGNPVEKERDARDIVEKIRGYKYLIVSKVEKKHHILHPPPPFNLGDLQKEAA
CCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEHHHHHCCCCCCCCCCCHHHHHHHHH
RLYGFSPAETQSIAEQLYLDALISYPRTNSQKLPPTLDYRRILENISSIKSYRNLVESLI
HHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SETKGVLTPVEGEKEDPAHPAIYPTGVVPGRLTEKQWAIYDLIVKRFLAVFAPPAKIVYY
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHEEEE
VVKATTPSHEYVFETKGQVIEEYGWLKYYNFHNYHAGKIPVLKEGELLNIVKATASESYT
EEEECCCCCCEEECCCHHHHHHCCCEEEECCCCCCCCCCCEECCCCHHHHHHHHHCCCCC
KPPKRLTKTDLLRWMESVEIGTEATRAQIIEKLFERKYLYQHQKSSGIDVSEIGFGVVEI
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LEKFFPDLLSVELTRKFEKEMDSIRSGVKRRSEVLEEARRVLTELLGKFDRNRIEAGKQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALRLKIIQNTRKCRVSSCYREGVVNGLCSHHANAVKLVREYYGEWSRRKDITFNEYIDKL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHH
RKSKLTGKWVKEVIESELT
HHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MVSKSIRIWDLKGRILVIAEKPKAAKKIASALSPTHNAKRIGSITIYEVDSYSSSIIIAS
CCCCCEEEEEECCCEEEEECCCHHHHHHHHHHCCCCCHHHCCCEEEEEECCCCCCEEEEE
SVGHLYQLHTSLRGYPVYSYEWIPLHLTDPSKKYIREYLEVLGKLCRGVDYYVNACDYDI
HHHHHHHHHHHCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCC
EGSVIGYLIIKFNGDERKAFRARFSSLTIEELRKAFNNLTRLDYEMIEAGLCRHELDWIW
CCCEEEEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
GINVSRALMKAVEDAVGERIILSAGRVQTPTLKYVVDKVVERNLFIPLPQYNINITVEKN
CCCHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCEEECCCCEEEEEEECC
GEKIRLEYTGNPVEKERDARDIVEKIRGYKYLIVSKVEKKHHILHPPPPFNLGDLQKEAA
CCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEHHHHHCCCCCCCCCCCHHHHHHHHH
RLYGFSPAETQSIAEQLYLDALISYPRTNSQKLPPTLDYRRILENISSIKSYRNLVESLI
HHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SETKGVLTPVEGEKEDPAHPAIYPTGVVPGRLTEKQWAIYDLIVKRFLAVFAPPAKIVYY
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHEEEE
VVKATTPSHEYVFETKGQVIEEYGWLKYYNFHNYHAGKIPVLKEGELLNIVKATASESYT
EEEECCCCCCEEECCCHHHHHHCCCEEEECCCCCCCCCCCEECCCCHHHHHHHHHCCCCC
KPPKRLTKTDLLRWMESVEIGTEATRAQIIEKLFERKYLYQHQKSSGIDVSEIGFGVVEI
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LEKFFPDLLSVELTRKFEKEMDSIRSGVKRRSEVLEEARRVLTELLGKFDRNRIEAGKQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALRLKIIQNTRKCRVSSCYREGVVNGLCSHHANAVKLVREYYGEWSRRKDITFNEYIDKL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHH
RKSKLTGKWVKEVIESELT
HHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]