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The map label for this gene is priB [H]

Identifier: 218884209

GI number: 218884209

Start: 855787

End: 856932

Strand: Reverse

Name: priB [H]

Synonym: DKAM_0898

Alternate gene names: 218884209

Gene position: 856932-855787 (Counterclockwise)

Preceding gene: 218884210

Following gene: 218884208

Centisome position: 62.77

GC content: 44.76

Gene sequence:

>1146_bases
ATGGCTCACATAGACTTGATTAAATACCCCTTTGTGGCTGGCCCCAGCGACTTTAGTCCAAGTAGGTTTGGATTACCTCG
GGACACTGATTCCTCAACGATTATATCGATAATCATTAGAGGCGCCCTCGACAGGCTTCGCGAAATATTTCCATTAGTTC
TCACAGATATTCTCTCAGAGAGAATGCGGGTTAACACGAAGATTTTCATGGATGAATATGAGAGTATCGCATGTTATAAG
CTTCTTCTAGCGGTGGCTAAATCCATCGGGGATAGGAAACTAATTAATAGGATTGCCCTAGCATACGCTAAGTCAGCGAG
GAGAAGTCTCTTCAAAGAGGATGACTCCGTCATCCTCTCCATAGCATCAAAAATAGGTCTCCACCTAGTGTACGTCGAGA
ATCCACCTGCGCTCCCGGTATTCACTAATAATGTTGGCGGGGGGAAGAATAGGGCTAGAGGTGCATTCTTACCGCTTAAC
TATTCCCTCCCGGTGATAGAGTTCACGGGGATCGTGGCAGAGAGGCTTTCACAGGACCCCGCATACTCTTTAGAGAATAA
TGTTGTCTCGGAGGGGAAGGTTTATATGAATAAGAGAATGATTCTGAGAATCCTTGAGGAAGCGGTGATCAAGCATATAA
TCACTGATGCTGCGTCTATGGATATTAGTTCGCTGAATATACCTCTCCAGGATTTCATTGATAAGGCCAGGGAGAAACTA
GTAGATGCCGGGTGGTTTAAATCCATCACTGCGAGAGGAACCATGGAAACCAGTGAAGTAGGCGGCCTGATCACCGAAGC
CCTTCCACCATGTATATCTAGGATAATTAGTATTGTTGAGTCAGGAGGGAACCCGAGTCATGAGGAGAGATTTAACCTTG
CAGCATTTCTAGCTAATATAGGTCTCGACGTGGATTCTATACTGGAGTATTTCAGGAAGACACCGGATTTCAATGAGAAA
ATAGCCAGGTACCAAGTAGAACATATCTCGGGTATGAAGGGTAGTAGGAAGAAGTACATGCCTTACAGCTGTGAGAAAAT
GAAGTCTACGAATATATGTCCTATAAGTGATAGGTGCAAGGGCGGCAGGAACCCATTGTCTGTATACAGGTATAATTTGA
AGCGTGGTTTAAAGCGGGAAAGATGA

Upstream 100 bases:

>100_bases
CTGAAGAAACATATATGCCCAAAACATGTTCACATTGTTACAATAATATAACCACGATTATATCTCTCGGTATTTATACA
ACTAGGTAAAGGGAGGTAGA

Downstream 100 bases:

>100_bases
CATCATAGTACTCCTTGATGAGCCAGTTGATCACGGAATATACTATATATAAGACCGCGGAGACAGTCATTATAGTGAAA
ATAATCGAATCCATGCCCGG

Product: DNA primase, large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MAHIDLIKYPFVAGPSDFSPSRFGLPRDTDSSTIISIIIRGALDRLREIFPLVLTDILSERMRVNTKIFMDEYESIACYK
LLLAVAKSIGDRKLINRIALAYAKSARRSLFKEDDSVILSIASKIGLHLVYVENPPALPVFTNNVGGGKNRARGAFLPLN
YSLPVIEFTGIVAERLSQDPAYSLENNVVSEGKVYMNKRMILRILEEAVIKHIITDAASMDISSLNIPLQDFIDKAREKL
VDAGWFKSITARGTMETSEVGGLITEALPPCISRIISIVESGGNPSHEERFNLAAFLANIGLDVDSILEYFRKTPDFNEK
IARYQVEHISGMKGSRKKYMPYSCEKMKSTNICPISDRCKGGRNPLSVYRYNLKRGLKRER

Sequences:

>Translated_381_residues
MAHIDLIKYPFVAGPSDFSPSRFGLPRDTDSSTIISIIIRGALDRLREIFPLVLTDILSERMRVNTKIFMDEYESIACYK
LLLAVAKSIGDRKLINRIALAYAKSARRSLFKEDDSVILSIASKIGLHLVYVENPPALPVFTNNVGGGKNRARGAFLPLN
YSLPVIEFTGIVAERLSQDPAYSLENNVVSEGKVYMNKRMILRILEEAVIKHIITDAASMDISSLNIPLQDFIDKAREKL
VDAGWFKSITARGTMETSEVGGLITEALPPCISRIISIVESGGNPSHEERFNLAAFLANIGLDVDSILEYFRKTPDFNEK
IARYQVEHISGMKGSRKKYMPYSCEKMKSTNICPISDRCKGGRNPLSVYRYNLKRGLKRER
>Mature_380_residues
AHIDLIKYPFVAGPSDFSPSRFGLPRDTDSSTIISIIIRGALDRLREIFPLVLTDILSERMRVNTKIFMDEYESIACYKL
LLAVAKSIGDRKLINRIALAYAKSARRSLFKEDDSVILSIASKIGLHLVYVENPPALPVFTNNVGGGKNRARGAFLPLNY
SLPVIEFTGIVAERLSQDPAYSLENNVVSEGKVYMNKRMILRILEEAVIKHIITDAASMDISSLNIPLQDFIDKAREKLV
DAGWFKSITARGTMETSEVGGLITEALPPCISRIISIVESGGNPSHEERFNLAAFLANIGLDVDSILEYFRKTPDFNEKI
ARYQVEHISGMKGSRKKYMPYSCEKMKSTNICPISDRCKGGRNPLSVYRYNLKRGLKRER

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG2219

COG function: function code L; Eukaryotic-type DNA primase, large subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic-type primase large subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007238 [H]

Pfam domain/function: PF04104 DNA_primase_lrg [H]

EC number: NA

Molecular weight: Translated: 42703; Mature: 42572

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHIDLIKYPFVAGPSDFSPSRFGLPRDTDSSTIISIIIRGALDRLREIFPLVLTDILSE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMRVNTKIFMDEYESIACYKLLLAVAKSIGDRKLINRIALAYAKSARRSLFKEDDSVILS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
IASKIGLHLVYVENPPALPVFTNNVGGGKNRARGAFLPLNYSLPVIEFTGIVAERLSQDP
HHHHHCEEEEEECCCCCCCEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCC
AYSLENNVVSEGKVYMNKRMILRILEEAVIKHIITDAASMDISSLNIPLQDFIDKAREKL
CHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHH
VDAGWFKSITARGTMETSEVGGLITEALPPCISRIISIVESGGNPSHEERFNLAAFLANI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GLDVDSILEYFRKTPDFNEKIARYQVEHISGMKGSRKKYMPYSCEKMKSTNICPISDRCK
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCHHHCC
GGRNPLSVYRYNLKRGLKRER
CCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AHIDLIKYPFVAGPSDFSPSRFGLPRDTDSSTIISIIIRGALDRLREIFPLVLTDILSE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMRVNTKIFMDEYESIACYKLLLAVAKSIGDRKLINRIALAYAKSARRSLFKEDDSVILS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
IASKIGLHLVYVENPPALPVFTNNVGGGKNRARGAFLPLNYSLPVIEFTGIVAERLSQDP
HHHHHCEEEEEECCCCCCCEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCC
AYSLENNVVSEGKVYMNKRMILRILEEAVIKHIITDAASMDISSLNIPLQDFIDKAREKL
CHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHH
VDAGWFKSITARGTMETSEVGGLITEALPPCISRIISIVESGGNPSHEERFNLAAFLANI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GLDVDSILEYFRKTPDFNEKIARYQVEHISGMKGSRKKYMPYSCEKMKSTNICPISDRCK
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCHHHCC
GGRNPLSVYRYNLKRGLKRER
CCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]