Click here to switch to the map view.

The map label for this gene is gatE

Identifier: 218884131

GI number: 218884131

Start: 788409

End: 790325

Strand: Direct

Name: gatE

Synonym: DKAM_0820

Alternate gene names: 218884131

Gene position: 788409-790325 (Clockwise)

Preceding gene: 218884130

Following gene: 218884132

Centisome position: 57.75

GC content: 46.27

Gene sequence:

>1917_bases
ATGAGTAGGATCAACTATAGTGAAACCGGGTTAAAGGTCGGCCTCGAGATACATGTCCAATTAGATACGGCGAGGAAGCT
CTTCTGTAAATGCCCAACCCTCCTCAGTGAGGCAGAGGCCCGAGAAGTATTCACGAGATACCTTAGGCCGGCTAAGAGCG
AGGTAGGCGAGATAGATAGGGCTGCACTGCTTGAGTGGAAGAAGAACAAGAAGTATGAATACGAGGCCCCTGTGGAGTCG
AGCTGCCTTGTTGAAGCCGATGAGGAGCCGCCCCACGAGATAAATGAGGATGCATTACTAGTTGCATTAGCATTAGCCAG
CTCCATGAATATGAGGATCATTAATGAAATACATGTGATGAGGAAGATGGTTATCGATGGTAGTAATGTCAGTGGTTTCC
AGCGTACAGCGTTAATCGCTGTAAACGGTTACATAATGGATAACGAGGAGAAAATAGGCATACAGACACTATGCCTGGAG
GAGGATGCAGCTAGGAAGATCGATGAGTCTGAGACCACTGTAAGATATAGAATCGACAGGCTTGGCATTCCACTGGTAGA
GGTAGCTACGGCGCCGGATATACATGACCCGGAGCAAGCCGTCAGGGTAGCATTGAAAATAGGACAGTTAATCAGGCTCA
CGGGAAGGGCTAAACGAGGCCTGGGAACCATTAGGCAGGATCTAAACATAAGTATCAAGGGCGGTGCAAAAGTAGAGATT
AAAGGAGTGCAACACTTATATTTGATCTCAAAAGTGATAGAGTACGAGGTGTTAAGGCAGCTTAAACTCCTCGAGATACG
TGACGAGCTAGTTAAGCGTGGCGTGAGTCCGGAGAACATATCAGATAATATAATAGATGTCACAGATGTATTCAGGGATA
CAGGTTCGAAGATAGTGAAGAAGGCTCTCTCAATACCTGGGGGCGGTGTATACGCTGTTGTATTACGGGGCTTTAAAGGA
TTACTTGGGATAGAAATTCAGCCTGGGAGAAGATTTGGAAGCGAACTAGCCGACTATGCTAGGATCTGGGGAGGGGTTGG
CGGAATCTTTCACACGGATGAGTTGCCAAACTATGGGATCACTAGGGAAGAGGTTCAGGCTTTATACATGAAGACAGGGG
CTGATCCAGGTATAGATGCCATAGTAATAGTAGCAGATGTCAAGCAGAAGGCTGTGGAAGCATTAAAGGCGGTGGTCGAG
AGAGCCAGGATAGCTGTTATAGGTGTACCTGAGGAAACACGTGCCGCTAACCCGGATGGTACAACGAAATACATGCGTCC
ACGCCCCGGCTCAGCCAGGATGTATCCAGAGACAGATATACCACCCATATATATTACCCCAGAACTCATGGAGAAAGCTA
GCAAATTAATCCCAGAGCCCCCTGAGAAGAAGTTGGAGAAGTATGTAAGGGAGCATGGATTAAGCACGGAACTAGCTAAA
GCAGTAATTAATGATTTAAGGCTCGACCTCTATGAGAGACTAGTGGAGAAATACATGGGTAAGATACCAGCATCAATTAT
AGCGTCAACACTGGTCAACATTATTCCCTCATTGAGGCGTGACGGGGTACCCATCGAGAATATCGATGACTACGTGATCG
AGGATGTCCTGGATGCGGTGGCAAACAATGAGATAGCTAAAGAGGCCATACCAGATGTATTGTCAAGTATAGCGAGGAAC
CCGGGCAAGAGCATTAAGGATGTTATAAAAGAGCTTGGACTAGGCGTTGTCTCAATCAGTGAGCTAGACGAGTTGATTAA
TGAAGCAATAAAGAAGAACATGGATAAGATCACCGCTAAAAGGGAGAAGGCCTTCCAGATAGTTATGAGCGAGGTCATGA
AGTCTGTTAGGGGTAGAATAGATGGAGCACTCGTAGCTGAAAAGGTTAGAGAGAAATTAAAGGAGATACTGAAATAA

Upstream 100 bases:

>100_bases
GTATCAAGGGACCTTGAGGAAGTTAGGAAAGTTTTCCTCACGAATCTCGCTGGAGAATTCAATGAGAGGCATATACTAGA
CCTCTACCCGAGGTGGAATC

Downstream 100 bases:

>100_bases
AATCATGCAGCGATGACGAGTACTCCCGTCAATGAGTGGTGATTAGAAGGCATTTGGCTGAGCCGTTTATCTCCACAGCT
TTCACTCTATTTAAGATAAG

Product: glutamyl-tRNA(Gln) amidotransferase subunit E

Products: NA

Alternate protein names: Glu-ADT subunit E

Number of amino acids: Translated: 638; Mature: 637

Protein sequence:

>638_residues
MSRINYSETGLKVGLEIHVQLDTARKLFCKCPTLLSEAEAREVFTRYLRPAKSEVGEIDRAALLEWKKNKKYEYEAPVES
SCLVEADEEPPHEINEDALLVALALASSMNMRIINEIHVMRKMVIDGSNVSGFQRTALIAVNGYIMDNEEKIGIQTLCLE
EDAARKIDESETTVRYRIDRLGIPLVEVATAPDIHDPEQAVRVALKIGQLIRLTGRAKRGLGTIRQDLNISIKGGAKVEI
KGVQHLYLISKVIEYEVLRQLKLLEIRDELVKRGVSPENISDNIIDVTDVFRDTGSKIVKKALSIPGGGVYAVVLRGFKG
LLGIEIQPGRRFGSELADYARIWGGVGGIFHTDELPNYGITREEVQALYMKTGADPGIDAIVIVADVKQKAVEALKAVVE
RARIAVIGVPEETRAANPDGTTKYMRPRPGSARMYPETDIPPIYITPELMEKASKLIPEPPEKKLEKYVREHGLSTELAK
AVINDLRLDLYERLVEKYMGKIPASIIASTLVNIIPSLRRDGVPIENIDDYVIEDVLDAVANNEIAKEAIPDVLSSIARN
PGKSIKDVIKELGLGVVSISELDELINEAIKKNMDKITAKREKAFQIVMSEVMKSVRGRIDGALVAEKVREKLKEILK

Sequences:

>Translated_638_residues
MSRINYSETGLKVGLEIHVQLDTARKLFCKCPTLLSEAEAREVFTRYLRPAKSEVGEIDRAALLEWKKNKKYEYEAPVES
SCLVEADEEPPHEINEDALLVALALASSMNMRIINEIHVMRKMVIDGSNVSGFQRTALIAVNGYIMDNEEKIGIQTLCLE
EDAARKIDESETTVRYRIDRLGIPLVEVATAPDIHDPEQAVRVALKIGQLIRLTGRAKRGLGTIRQDLNISIKGGAKVEI
KGVQHLYLISKVIEYEVLRQLKLLEIRDELVKRGVSPENISDNIIDVTDVFRDTGSKIVKKALSIPGGGVYAVVLRGFKG
LLGIEIQPGRRFGSELADYARIWGGVGGIFHTDELPNYGITREEVQALYMKTGADPGIDAIVIVADVKQKAVEALKAVVE
RARIAVIGVPEETRAANPDGTTKYMRPRPGSARMYPETDIPPIYITPELMEKASKLIPEPPEKKLEKYVREHGLSTELAK
AVINDLRLDLYERLVEKYMGKIPASIIASTLVNIIPSLRRDGVPIENIDDYVIEDVLDAVANNEIAKEAIPDVLSSIARN
PGKSIKDVIKELGLGVVSISELDELINEAIKKNMDKITAKREKAFQIVMSEVMKSVRGRIDGALVAEKVREKLKEILK
>Mature_637_residues
SRINYSETGLKVGLEIHVQLDTARKLFCKCPTLLSEAEAREVFTRYLRPAKSEVGEIDRAALLEWKKNKKYEYEAPVESS
CLVEADEEPPHEINEDALLVALALASSMNMRIINEIHVMRKMVIDGSNVSGFQRTALIAVNGYIMDNEEKIGIQTLCLEE
DAARKIDESETTVRYRIDRLGIPLVEVATAPDIHDPEQAVRVALKIGQLIRLTGRAKRGLGTIRQDLNISIKGGAKVEIK
GVQHLYLISKVIEYEVLRQLKLLEIRDELVKRGVSPENISDNIIDVTDVFRDTGSKIVKKALSIPGGGVYAVVLRGFKGL
LGIEIQPGRRFGSELADYARIWGGVGGIFHTDELPNYGITREEVQALYMKTGADPGIDAIVIVADVKQKAVEALKAVVER
ARIAVIGVPEETRAANPDGTTKYMRPRPGSARMYPETDIPPIYITPELMEKASKLIPEPPEKKLEKYVREHGLSTELAKA
VINDLRLDLYERLVEKYMGKIPASIIASTLVNIIPSLRRDGVPIENIDDYVIEDVLDAVANNEIAKEAIPDVLSSIARNP
GKSIKDVIKELGLGVVSISELDELINEAIKKNMDKITAKREKAFQIVMSEVMKSVRGRIDGALVAEKVREKLKEILK

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG2511

COG function: function code J; Archaeal Glu-tRNAGln amidotransferase subunit E (contains GAD domain)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatE subfamily

Homologues:

Organism=Saccharomyces cerevisiae, GI6319391, Length=279, Percent_Identity=27.2401433691756, Blast_Score=70, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATE_DESK1 (B8D4W5)

Other databases:

- EMBL:   CP001140
- RefSeq:   YP_002428513.1
- GeneID:   7170970
- GenomeReviews:   CP001140_GR
- KEGG:   dka:DKAM_0820
- HOGENOM:   HBG297405
- OMA:   TSGFQRT
- ProtClustDB:   PRK04028
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00588
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004115
- InterPro:   IPR023168
- InterPro:   IPR004414
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00134

Pfam domain/function: PF02938 GAD; PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 70861; Mature: 70730

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRINYSETGLKVGLEIHVQLDTARKLFCKCPTLLSEAEAREVFTRYLRPAKSEVGEIDR
CCCCCCCCCCEEEEEEEEEEEHHHHHHHHHCHHHHHHHHHHHHHHHHHCHHHHHHCCHHH
AALLEWKKNKKYEYEAPVESSCLVEADEEPPHEINEDALLVALALASSMNMRIINEIHVM
HHHHHHHCCCCCEECCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
RKMVIDGSNVSGFQRTALIAVNGYIMDNEEKIGIQTLCLEEDAARKIDESETTVRYRIDR
HHHHHCCCCCCCCHHEEEEEEEEEEECCCCCCCHHEEEECCHHHHHCCCCCHHHHEEHHH
LGIPLVEVATAPDIHDPEQAVRVALKIGQLIRLTGRAKRGLGTIRQDLNISIKGGAKVEI
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEE
KGVQHLYLISKVIEYEVLRQLKLLEIRDELVKRGVSPENISDNIIDVTDVFRDTGSKIVK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KALSIPGGGVYAVVLRGFKGLLGIEIQPGRRFGSELADYARIWGGVGGIFHTDELPNYGI
HHHCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
TREEVQALYMKTGADPGIDAIVIVADVKQKAVEALKAVVERARIAVIGVPEETRAANPDG
CHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCEEEEECCCHHCCCCCCC
TTKYMRPRPGSARMYPETDIPPIYITPELMEKASKLIPEPPEKKLEKYVREHGLSTELAK
CCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHH
AVINDLRLDLYERLVEKYMGKIPASIIASTLVNIIPSLRRDGVPIENIDDYVIEDVLDAV
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
ANNEIAKEAIPDVLSSIARNPGKSIKDVIKELGLGVVSISELDELINEAIKKNMDKITAK
HCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHH
REKAFQIVMSEVMKSVRGRIDGALVAEKVREKLKEILK
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SRINYSETGLKVGLEIHVQLDTARKLFCKCPTLLSEAEAREVFTRYLRPAKSEVGEIDR
CCCCCCCCCEEEEEEEEEEEHHHHHHHHHCHHHHHHHHHHHHHHHHHCHHHHHHCCHHH
AALLEWKKNKKYEYEAPVESSCLVEADEEPPHEINEDALLVALALASSMNMRIINEIHVM
HHHHHHHCCCCCEECCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
RKMVIDGSNVSGFQRTALIAVNGYIMDNEEKIGIQTLCLEEDAARKIDESETTVRYRIDR
HHHHHCCCCCCCCHHEEEEEEEEEEECCCCCCCHHEEEECCHHHHHCCCCCHHHHEEHHH
LGIPLVEVATAPDIHDPEQAVRVALKIGQLIRLTGRAKRGLGTIRQDLNISIKGGAKVEI
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEE
KGVQHLYLISKVIEYEVLRQLKLLEIRDELVKRGVSPENISDNIIDVTDVFRDTGSKIVK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KALSIPGGGVYAVVLRGFKGLLGIEIQPGRRFGSELADYARIWGGVGGIFHTDELPNYGI
HHHCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
TREEVQALYMKTGADPGIDAIVIVADVKQKAVEALKAVVERARIAVIGVPEETRAANPDG
CHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCEEEEECCCHHCCCCCCC
TTKYMRPRPGSARMYPETDIPPIYITPELMEKASKLIPEPPEKKLEKYVREHGLSTELAK
CCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHH
AVINDLRLDLYERLVEKYMGKIPASIIASTLVNIIPSLRRDGVPIENIDDYVIEDVLDAV
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
ANNEIAKEAIPDVLSSIARNPGKSIKDVIKELGLGVVSISELDELINEAIKKNMDKITAK
HCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHH
REKAFQIVMSEVMKSVRGRIDGALVAEKVREKLKEILK
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA