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The map label for this gene is gspE [C]

Identifier: 218883477

GI number: 218883477

Start: 150330

End: 152030

Strand: Reverse

Name: gspE [C]

Synonym: DKAM_0163

Alternate gene names: 218883477

Gene position: 152030-150330 (Counterclockwise)

Preceding gene: 218883485

Following gene: 218883475

Centisome position: 11.14

GC content: 45.86

Gene sequence:

>1701_bases
ATGGAGGTATCAAGCTTGAAATTAAAGATGCCATTGATTAAGCTGAGGAAGGAACCTGTGGAAAAGAAATCCGAAGTGAA
GAAAGCGAAGATATTAAAGGAGAGTATTACAGCTATCGAGGAACTAGTACAGGTATCGACGAGAGATCCGTCCTGGCGCA
TTCTCGATTCATACTACATATATAAGCCATTCGTTAAGATAACCATTGCTGAAACACCTAAGGGACCCATGTACTTCGTG
GAGGAGCAGGGGCTGGACGAAGAAGATAAGATAGCTCTGGAGAAACTCTACGAGATCCTTATGGATGAGATAAGACCGCC
CCAGAGAAGAGAGGATCTGGCTGATCTAAGGGGATACGTGTTTAAGGAGGTCGAGAGGATCAGTGAGAAATACCGTGAGA
AACTGGGTATGGTTGGATCTAGAAGGCTTAAGGTGATGTATTATATCGAGAGGAATCTCCTCGGTTACGGCGTACTAGAC
CCGATAATGAAGGATCCCAATATAGAGGATATCTCCTGCAACGGCGTGGGGATACCGGTATTCGTCTGGCACAGGAAGTA
CGAGAGCATACCCTCCAACATCACATTCACCGCTGAGGAAGCATTGAATGAGCTGATAATGAAAATGGCTCATATGGCGG
GTAAGCATATAAGCATAGCATACCCTATTCTAGACGCAATGCTACCCGAGAAACACCGTGTTGCAGCAACCTACGGTAGA
GAGGTTTCCACCAAGGGGCCATCCTTCACTATACGTAAGTTCCGTGAGAAACCATTCAGCATCATAGAAGTTATCGAGCA
GGGTAACATAGATGCTTTATCTGCAGCATACCTATGGATGCTTCTAGAGCATGGTAAGACATTCATGATTGCTGGTGGCA
CCGGTACAGGTAAAACAACAATGCTGAACGCGCTTTCAATGTTCATTAAGCCAGGTATGAAAATAGTGACAATCGAGGAT
ACACCAGAACTAAACCTGCCCCATGTCAACTGGGTTCAGTTAACCAGCAGGGAAACCTATGTTGTGGGAACGCAGAGTCT
TGGGACAAGTGTGAAGCTCTATGACCTGGTAAAGTTAAGCCTTAGATATAGGCCTGATTACGTGATTGTTGGAGAGGTGC
GTGGCGAGGAGGCATCTGTATTATTCCAGGCAATGGCAACTGGGCACAGTGGTGCCAGCACCATCCATGCTGAAACACTT
GACTACGCTATTAAAAGACTTACAAGCCCTCCGATGAATATCTCTCCAGCATATATGAAGCTTATGAATGTATTTATGCA
TATCCAGAGAGTGATCACTAGGGTGGAGAAAGGAGTGGTAAAGGTTAGGAGAAGAATAACCGTGGTACAGGAGGTCGAGG
ACTTTGAAAAATATAGGTCGATAAGCGTATGGGATCCGAGAACAGATACACATAAAGTAGGCCTGAAAGAGAGCATCCAC
CTAGAGGATGTCGCGGCAAAGAAAGGATGGGACCTGGAAGATGTCATAGAGGAGATAAAGAGGAGAGCACTTGTCCTCGA
GTGGATGAAGGAGGAGGGGATTAAAGATGCCTGGGATGTCTCAAGAATAATCTTTGACTACTACTATGAGCCGCAACTAG
TGTATGAGAAGGCTGTTAAACGATTGACAGAGATAAGAGGGGAGAGTAAGGAGAAGATCAGTGAGGAGATCACAGCAGAA
ACCAGGCCTGACGCCGGGTAG

Upstream 100 bases:

>100_bases
CTTAACTTTAACTTAAAAATGCAGGCTTATTTATATTATTAACTAGTCTAATAAATATTTTGTAAGAAGTATACCCGTTA
AACATAGCTAATATTTAGAT

Downstream 100 bases:

>100_bases
ATTTAATAGCATTGAATCTCGCACCTAGAGGTTAGCCGATTAGTTGAAACTCTATTCTAACCTCCAAGCCCACCTCATGT
AACTCCTTTAGTATCGGTAA

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MEVSSLKLKMPLIKLRKEPVEKKSEVKKAKILKESITAIEELVQVSTRDPSWRILDSYYIYKPFVKITIAETPKGPMYFV
EEQGLDEEDKIALEKLYEILMDEIRPPQRREDLADLRGYVFKEVERISEKYREKLGMVGSRRLKVMYYIERNLLGYGVLD
PIMKDPNIEDISCNGVGIPVFVWHRKYESIPSNITFTAEEALNELIMKMAHMAGKHISIAYPILDAMLPEKHRVAATYGR
EVSTKGPSFTIRKFREKPFSIIEVIEQGNIDALSAAYLWMLLEHGKTFMIAGGTGTGKTTMLNALSMFIKPGMKIVTIED
TPELNLPHVNWVQLTSRETYVVGTQSLGTSVKLYDLVKLSLRYRPDYVIVGEVRGEEASVLFQAMATGHSGASTIHAETL
DYAIKRLTSPPMNISPAYMKLMNVFMHIQRVITRVEKGVVKVRRRITVVQEVEDFEKYRSISVWDPRTDTHKVGLKESIH
LEDVAAKKGWDLEDVIEEIKRRALVLEWMKEEGIKDAWDVSRIIFDYYYEPQLVYEKAVKRLTEIRGESKEKISEEITAE
TRPDAG

Sequences:

>Translated_566_residues
MEVSSLKLKMPLIKLRKEPVEKKSEVKKAKILKESITAIEELVQVSTRDPSWRILDSYYIYKPFVKITIAETPKGPMYFV
EEQGLDEEDKIALEKLYEILMDEIRPPQRREDLADLRGYVFKEVERISEKYREKLGMVGSRRLKVMYYIERNLLGYGVLD
PIMKDPNIEDISCNGVGIPVFVWHRKYESIPSNITFTAEEALNELIMKMAHMAGKHISIAYPILDAMLPEKHRVAATYGR
EVSTKGPSFTIRKFREKPFSIIEVIEQGNIDALSAAYLWMLLEHGKTFMIAGGTGTGKTTMLNALSMFIKPGMKIVTIED
TPELNLPHVNWVQLTSRETYVVGTQSLGTSVKLYDLVKLSLRYRPDYVIVGEVRGEEASVLFQAMATGHSGASTIHAETL
DYAIKRLTSPPMNISPAYMKLMNVFMHIQRVITRVEKGVVKVRRRITVVQEVEDFEKYRSISVWDPRTDTHKVGLKESIH
LEDVAAKKGWDLEDVIEEIKRRALVLEWMKEEGIKDAWDVSRIIFDYYYEPQLVYEKAVKRLTEIRGESKEKISEEITAE
TRPDAG
>Mature_566_residues
MEVSSLKLKMPLIKLRKEPVEKKSEVKKAKILKESITAIEELVQVSTRDPSWRILDSYYIYKPFVKITIAETPKGPMYFV
EEQGLDEEDKIALEKLYEILMDEIRPPQRREDLADLRGYVFKEVERISEKYREKLGMVGSRRLKVMYYIERNLLGYGVLD
PIMKDPNIEDISCNGVGIPVFVWHRKYESIPSNITFTAEEALNELIMKMAHMAGKHISIAYPILDAMLPEKHRVAATYGR
EVSTKGPSFTIRKFREKPFSIIEVIEQGNIDALSAAYLWMLLEHGKTFMIAGGTGTGKTTMLNALSMFIKPGMKIVTIED
TPELNLPHVNWVQLTSRETYVVGTQSLGTSVKLYDLVKLSLRYRPDYVIVGEVRGEEASVLFQAMATGHSGASTIHAETL
DYAIKRLTSPPMNISPAYMKLMNVFMHIQRVITRVEKGVVKVRRRITVVQEVEDFEKYRSISVWDPRTDTHKVGLKESIH
LEDVAAKKGWDLEDVIEEIKRRALVLEWMKEEGIKDAWDVSRIIFDYYYEPQLVYEKAVKRLTEIRGESKEKISEEITAE
TRPDAG

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG0630

COG function: function code NU; Type IV secretory pathway, VirB11 components, and related ATPases involved in archaeal flagella biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=130, Percent_Identity=35.3846153846154, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 65039; Mature: 65039

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVSSLKLKMPLIKLRKEPVEKKSEVKKAKILKESITAIEELVQVSTRDPSWRILDSYYI
CCCCCEEEECHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEE
YKPFVKITIAETPKGPMYFVEEQGLDEEDKIALEKLYEILMDEIRPPQRREDLADLRGYV
EECEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FKEVERISEKYREKLGMVGSRRLKVMYYIERNLLGYGVLDPIMKDPNIEDISCNGVGIPV
HHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHCCCCCCEEECCCCCCEE
FVWHRKYESIPSNITFTAEEALNELIMKMAHMAGKHISIAYPILDAMLPEKHRVAATYGR
EEEECHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCHHHHHHHCC
EVSTKGPSFTIRKFREKPFSIIEVIEQGNIDALSAAYLWMLLEHGKTFMIAGGTGTGKTT
CCCCCCCCHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHH
MLNALSMFIKPGMKIVTIEDTPELNLPHVNWVQLTSRETYVVGTQSLGTSVKLYDLVKLS
HHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEECCCEEEEECCCCCCCEEHHHHHHHH
LRYRPDYVIVGEVRGEEASVLFQAMATGHSGASTIHAETLDYAIKRLTSPPMNISPAYMK
HEECCCEEEEEECCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCCCCCCCCHHHHH
LMNVFMHIQRVITRVEKGVVKVRRRITVVQEVEDFEKYRSISVWDPRTDTHKVGLKESIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHCCCCCCCC
LEDVAAKKGWDLEDVIEEIKRRALVLEWMKEEGIKDAWDVSRIIFDYYYEPQLVYEKAVK
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
RLTEIRGESKEKISEEITAETRPDAG
HHHHHCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEVSSLKLKMPLIKLRKEPVEKKSEVKKAKILKESITAIEELVQVSTRDPSWRILDSYYI
CCCCCEEEECHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEE
YKPFVKITIAETPKGPMYFVEEQGLDEEDKIALEKLYEILMDEIRPPQRREDLADLRGYV
EECEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FKEVERISEKYREKLGMVGSRRLKVMYYIERNLLGYGVLDPIMKDPNIEDISCNGVGIPV
HHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHCCCCCCEEECCCCCCEE
FVWHRKYESIPSNITFTAEEALNELIMKMAHMAGKHISIAYPILDAMLPEKHRVAATYGR
EEEECHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCHHHHHHHCC
EVSTKGPSFTIRKFREKPFSIIEVIEQGNIDALSAAYLWMLLEHGKTFMIAGGTGTGKTT
CCCCCCCCHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHH
MLNALSMFIKPGMKIVTIEDTPELNLPHVNWVQLTSRETYVVGTQSLGTSVKLYDLVKLS
HHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEECCCEEEEECCCCCCCEEHHHHHHHH
LRYRPDYVIVGEVRGEEASVLFQAMATGHSGASTIHAETLDYAIKRLTSPPMNISPAYMK
HEECCCEEEEEECCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCCCCCCCCHHHHH
LMNVFMHIQRVITRVEKGVVKVRRRITVVQEVEDFEKYRSISVWDPRTDTHKVGLKESIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHCCCCCCCC
LEDVAAKKGWDLEDVIEEIKRRALVLEWMKEEGIKDAWDVSRIIFDYYYEPQLVYEKAVK
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
RLTEIRGESKEKISEEITAETRPDAG
HHHHHCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]