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The map label for this gene is thiC [H]

Identifier: 218883436

GI number: 218883436

Start: 109085

End: 110371

Strand: Reverse

Name: thiC [H]

Synonym: DKAM_0122

Alternate gene names: 218883436

Gene position: 110371-109085 (Counterclockwise)

Preceding gene: 218883437

Following gene: 218883434

Centisome position: 8.08

GC content: 46.23

Gene sequence:

>1287_bases
ATGGGTATTGCTAAATCCGGGAATACTACTGAGGAGATGAAGAGGCTCAGCGAGATAGAGGATGTTCCTGTTGAGAAGAT
CAGGGAGAGAATAGCTTCTGGTAGAATAGTTATTACTAGAAATATTAGACGTGAGAGTATAAGAACTACTGGGATAGGTG
AGGGATTATATACAAAGGTGAACGTGAATATTGGGACAAGCGGCATGCTAGTAGATATTGAAATGGAGAAGAAGAAGGCT
CGCATAGCCGTGAAATACGGTGCCGACACCATTATGGATCTAAGCACTGGGGGCAACCTAGATGAGATAAGGAGGATTCT
CTTAAAAGAGGTTGAGCCACTACCACTGGGCACAGTCCCAGCGTATCAAGCATGGATTGAAGGCGTTAAGAAATACGGAG
GCGTGAGCTTCCCGAGTGAGTGGTTTATCGAGATTGTTGAGAGACAGTTAAGGGATGGAGTTGACTTCATGACCATTCAC
TCAGCGCTATCCCTTGAACTAGCTAGGAAAGCAGTTGCTAGCACACGAATAATGCCGATTGTGAGCCGGGGTGGGGCAAT
GCTTGCTGTCTGGATGTTGGAGAACAATGAGGAGAACCCGTTTAGGCAACACTGGGACTACTTATTAGAATTGTTCAGGG
AGTATGATGCTGTTATAAGTATAGGGGACTCCCTAAGGCCGGGTACTATAATGGATCAACACGATGAGTTACAGGTAACC
GAGTTGATAGAGATAGCCAGACAGGTGAAACATGCTAGAGAGCACGATGTACAAGTGATCGTCGAGGGACCCGGACACAT
GACGCTGGATCAGATCACTAGTAACATCACTTTAATGAAGAAACTAACTAATAAAGCCCCCTATTACGTCCTAGGACCAC
TGGTCACCGATATAGCTATGGGCTACGACCATATAGCCGCCGCCATAGGCGGCGCCATAGCTGCTGCTCATGGAGCAGAC
TTCCTCTGTTATCTAACACCAGCAGAACATCTAAGTCTCCCAACCATAGAGCAGGTCAGGGAGGGATTAGTGGCCACTAG
GATCGCTGCACATGCAGGCGATATAGTGAAACTCGGCAAAAAAGCCTACCGTAGAGATGCTGAGCTAAGTGTTTTCAGAG
CTAGGTTGGAATGGGATAAGGTACTTGAATACGCTTTCGATATTGAGAAGGCCAGAGAGATCTATACACAGTTCCCAGTA
TCCACTAGGGGTTGCAATATGTGTGGACAATACTGTGTCTTCCTATTGCTCGATAGGTTATTGAAAAAGCCAGCATTAAA
ACAGTGA

Upstream 100 bases:

>100_bases
ATTAAAGAGAAGCCAGAGATCCTTGAGAAGCTTAAGGGGCTCAGCATGGAGTACCAGAACTACTTAAGGATGAGAGGAAA
GAACCGTGACTAAAACAATT

Downstream 100 bases:

>100_bases
GGGTATTGATGTAGTTTCAGGTATGAGGGTTTTAAGGTTTTTGTAGGTTCTTTAATGTCCTTCTAGCTCTCAGTGCTGTT
AGGTATGCTAATGCTGTAGA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MGIAKSGNTTEEMKRLSEIEDVPVEKIRERIASGRIVITRNIRRESIRTTGIGEGLYTKVNVNIGTSGMLVDIEMEKKKA
RIAVKYGADTIMDLSTGGNLDEIRRILLKEVEPLPLGTVPAYQAWIEGVKKYGGVSFPSEWFIEIVERQLRDGVDFMTIH
SALSLELARKAVASTRIMPIVSRGGAMLAVWMLENNEENPFRQHWDYLLELFREYDAVISIGDSLRPGTIMDQHDELQVT
ELIEIARQVKHAREHDVQVIVEGPGHMTLDQITSNITLMKKLTNKAPYYVLGPLVTDIAMGYDHIAAAIGGAIAAAHGAD
FLCYLTPAEHLSLPTIEQVREGLVATRIAAHAGDIVKLGKKAYRRDAELSVFRARLEWDKVLEYAFDIEKAREIYTQFPV
STRGCNMCGQYCVFLLLDRLLKKPALKQ

Sequences:

>Translated_428_residues
MGIAKSGNTTEEMKRLSEIEDVPVEKIRERIASGRIVITRNIRRESIRTTGIGEGLYTKVNVNIGTSGMLVDIEMEKKKA
RIAVKYGADTIMDLSTGGNLDEIRRILLKEVEPLPLGTVPAYQAWIEGVKKYGGVSFPSEWFIEIVERQLRDGVDFMTIH
SALSLELARKAVASTRIMPIVSRGGAMLAVWMLENNEENPFRQHWDYLLELFREYDAVISIGDSLRPGTIMDQHDELQVT
ELIEIARQVKHAREHDVQVIVEGPGHMTLDQITSNITLMKKLTNKAPYYVLGPLVTDIAMGYDHIAAAIGGAIAAAHGAD
FLCYLTPAEHLSLPTIEQVREGLVATRIAAHAGDIVKLGKKAYRRDAELSVFRARLEWDKVLEYAFDIEKAREIYTQFPV
STRGCNMCGQYCVFLLLDRLLKKPALKQ
>Mature_427_residues
GIAKSGNTTEEMKRLSEIEDVPVEKIRERIASGRIVITRNIRRESIRTTGIGEGLYTKVNVNIGTSGMLVDIEMEKKKAR
IAVKYGADTIMDLSTGGNLDEIRRILLKEVEPLPLGTVPAYQAWIEGVKKYGGVSFPSEWFIEIVERQLRDGVDFMTIHS
ALSLELARKAVASTRIMPIVSRGGAMLAVWMLENNEENPFRQHWDYLLELFREYDAVISIGDSLRPGTIMDQHDELQVTE
LIEIARQVKHAREHDVQVIVEGPGHMTLDQITSNITLMKKLTNKAPYYVLGPLVTDIAMGYDHIAAAIGGAIAAAHGADF
LCYLTPAEHLSLPTIEQVREGLVATRIAAHAGDIVKLGKKAYRRDAELSVFRARLEWDKVLEYAFDIEKAREIYTQFPVS
TRGCNMCGQYCVFLLLDRLLKKPALKQ

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=401, Percent_Identity=39.4014962593516, Blast_Score=289, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 47980; Mature: 47849

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIAKSGNTTEEMKRLSEIEDVPVEKIRERIASGRIVITRNIRRESIRTTGIGEGLYTKV
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCCEEEE
NVNIGTSGMLVDIEMEKKKARIAVKYGADTIMDLSTGGNLDEIRRILLKEVEPLPLGTVP
EEEECCCCEEEEEEECCCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCH
AYQAWIEGVKKYGGVSFPSEWFIEIVERQLRDGVDFMTIHSALSLELARKAVASTRIMPI
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VSRGGAMLAVWMLENNEENPFRQHWDYLLELFREYDAVISIGDSLRPGTIMDQHDELQVT
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHH
ELIEIARQVKHAREHDVQVIVEGPGHMTLDQITSNITLMKKLTNKAPYYVLGPLVTDIAM
HHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHH
GYDHIAAAIGGAIAAAHGADFLCYLTPAEHLSLPTIEQVREGLVATRIAAHAGDIVKLGK
HHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
KAYRRDAELSVFRARLEWDKVLEYAFDIEKAREIYTQFPVSTRGCNMCGQYCVFLLLDRL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
LKKPALKQ
HCCCCCCC
>Mature Secondary Structure 
GIAKSGNTTEEMKRLSEIEDVPVEKIRERIASGRIVITRNIRRESIRTTGIGEGLYTKV
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCCEEEE
NVNIGTSGMLVDIEMEKKKARIAVKYGADTIMDLSTGGNLDEIRRILLKEVEPLPLGTVP
EEEECCCCEEEEEEECCCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCH
AYQAWIEGVKKYGGVSFPSEWFIEIVERQLRDGVDFMTIHSALSLELARKAVASTRIMPI
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VSRGGAMLAVWMLENNEENPFRQHWDYLLELFREYDAVISIGDSLRPGTIMDQHDELQVT
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHH
ELIEIARQVKHAREHDVQVIVEGPGHMTLDQITSNITLMKKLTNKAPYYVLGPLVTDIAM
HHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHH
GYDHIAAAIGGAIAAAHGADFLCYLTPAEHLSLPTIEQVREGLVATRIAAHAGDIVKLGK
HHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
KAYRRDAELSVFRARLEWDKVLEYAFDIEKAREIYTQFPVSTRGCNMCGQYCVFLLLDRL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
LKKPALKQ
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA