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The map label for this gene is ilvE [H]

Identifier: 218711016

GI number: 218711016

Start: 3279409

End: 3280362

Strand: Direct

Name: ilvE [H]

Synonym: VS_3145

Alternate gene names: 218711016

Gene position: 3279409-3280362 (Clockwise)

Preceding gene: 218711015

Following gene: 218711017

Centisome position: 99.4

GC content: 48.01

Gene sequence:

>954_bases
ATGACAGCTAAAACAGCAGACTATATTTGGTTTAACGGTGAAATGGTTCCTTGGGCAGAGGCAAACGTTCACGTCCTGAC
TCACGCAATGCACTACGGTACTTCGGTGTTTGAAGGTGTTCGTTGTTACAACACGCCAAAGGGTCCGGTTATCTTCCGTC
ACCCAGAGCATGCAAGACGCCTAAAAGACTCAGCGAAAATCTACCGCTTCCCAATTCCTTACACAGAGGAAGAAATTATG
GAAGCGACTCGCGAAACGCTACGCCAAAATAAGCTAGAGTCAGCGTACATCCGCCCTCTAGGTTTCGTTGGTAACGTTGG
TTTGGGTGTCTGCCCGCCAGAAAACACTGAGATGGATCTGATCATCGCTGCGTTCCCTTGGGGTTCTTACCTAGGCGAAG
AAGCGCTAGAAAATGGCGTAGATGCGATGATATCAAGCTGGAATCGTGCAGCGCCAAATACCATCCCAACCGCAGCAAAA
GCCGGTGGTAACTACCTATCTTCACTACTGGTTGGTGGTGAAGCTCGTCGTCATGGTTACGATGAAGGTATCGCACTGAG
CGTCGATGGTTACCTTTCAGAAGGTGCGGGCGAGAACATCTTCGTGATTCGTAATGGCGTGCTATCAACACCACCAGCAA
CCAGCGCAATTCTGCCGGGTATTACTCGTGATTCGATCATGACGCTAGCAAAAGACATGGGTTATGAAATCCGTGAAGAG
AACATTGCTCGTGAAGCGCTATACCTTGCCGATGAAGTATTCATGACAGGCACAGCAGCAGAAATCGTTCCAGTTCGCAG
CGTAGACAAAATTACAGTAGGTGAAGGCAAACGCGGTCCTATCACTGAAAAAGTTCAAGCGGCTTACTTTGGTTTATTCA
ATGGAACCACTGAAGATAAGTGGGGCTGGTTAGATTATGTTTACCCAGAACACCAAACTTCAGCAAACAACTAA

Upstream 100 bases:

>100_bases
GCTGTGGGATGTGCGTACCGTTGACGTTATCTCGATAAGCCGTAATGAATTGCCAAACAACAACTTACAACAAAAGATAA
ACGCATAAGGAACCGCTAAC

Downstream 100 bases:

>100_bases
GTAAGTAACAGCCAAATATTTTATAAGGATTTAAGCAATGCCAATCTATCGTTCAGCAACGACTACCCACGGACGCAACA
TGGCTGGTGCGCGCGCTTTA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MTAKTADYIWFNGEMVPWAEANVHVLTHAMHYGTSVFEGVRCYNTPKGPVIFRHPEHARRLKDSAKIYRFPIPYTEEEIM
EATRETLRQNKLESAYIRPLGFVGNVGLGVCPPENTEMDLIIAAFPWGSYLGEEALENGVDAMISSWNRAAPNTIPTAAK
AGGNYLSSLLVGGEARRHGYDEGIALSVDGYLSEGAGENIFVIRNGVLSTPPATSAILPGITRDSIMTLAKDMGYEIREE
NIAREALYLADEVFMTGTAAEIVPVRSVDKITVGEGKRGPITEKVQAAYFGLFNGTTEDKWGWLDYVYPEHQTSANN

Sequences:

>Translated_317_residues
MTAKTADYIWFNGEMVPWAEANVHVLTHAMHYGTSVFEGVRCYNTPKGPVIFRHPEHARRLKDSAKIYRFPIPYTEEEIM
EATRETLRQNKLESAYIRPLGFVGNVGLGVCPPENTEMDLIIAAFPWGSYLGEEALENGVDAMISSWNRAAPNTIPTAAK
AGGNYLSSLLVGGEARRHGYDEGIALSVDGYLSEGAGENIFVIRNGVLSTPPATSAILPGITRDSIMTLAKDMGYEIREE
NIAREALYLADEVFMTGTAAEIVPVRSVDKITVGEGKRGPITEKVQAAYFGLFNGTTEDKWGWLDYVYPEHQTSANN
>Mature_316_residues
TAKTADYIWFNGEMVPWAEANVHVLTHAMHYGTSVFEGVRCYNTPKGPVIFRHPEHARRLKDSAKIYRFPIPYTEEEIME
ATRETLRQNKLESAYIRPLGFVGNVGLGVCPPENTEMDLIIAAFPWGSYLGEEALENGVDAMISSWNRAAPNTIPTAAKA
GGNYLSSLLVGGEARRHGYDEGIALSVDGYLSEGAGENIFVIRNGVLSTPPATSAILPGITRDSIMTLAKDMGYEIREEN
IAREALYLADEVFMTGTAAEIVPVRSVDKITVGEGKRGPITEKVQAAYFGLFNGTTEDKWGWLDYVYPEHQTSANN

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI50658084, Length=288, Percent_Identity=29.1666666666667, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI296010904, Length=329, Percent_Identity=25.2279635258359, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI38176287, Length=329, Percent_Identity=25.2279635258359, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI296010906, Length=329, Percent_Identity=25.2279635258359, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI258614015, Length=260, Percent_Identity=30, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI48994963, Length=307, Percent_Identity=71.3355048859935, Blast_Score=479, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17565728, Length=282, Percent_Identity=28.3687943262411, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17568601, Length=286, Percent_Identity=27.972027972028, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6322002, Length=287, Percent_Identity=27.8745644599303, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6322608, Length=331, Percent_Identity=26.2839879154079, Blast_Score=76, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24641779, Length=319, Percent_Identity=26.9592476489028, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34902; Mature: 34771

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKTADYIWFNGEMVPWAEANVHVLTHAMHYGTSVFEGVRCYNTPKGPVIFRHPEHARR
CCCCCCEEEEECCEECCCCCCCHHHHHHHHHHCHHHHHCHHCCCCCCCCEEEECCHHHHH
LKDSAKIYRFPIPYTEEEIMEATRETLRQNKLESAYIRPLGFVGNVGLGVCPPENTEMDL
HHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEE
IIAAFPWGSYLGEEALENGVDAMISSWNRAAPNTIPTAAKAGGNYLSSLLVGGEARRHGY
EEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHCCCHHHCCC
DEGIALSVDGYLSEGAGENIFVIRNGVLSTPPATSAILPGITRDSIMTLAKDMGYEIREE
CCCEEEEECCCCCCCCCCEEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCHHHHH
NIAREALYLADEVFMTGTAAEIVPVRSVDKITVGEGKRGPITEKVQAAYFGLFNGTTEDK
HHHHHHHHHHHHHHCCCCCHHEEECCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCC
WGWLDYVYPEHQTSANN
CCCEEEECCCCCCCCCC
>Mature Secondary Structure 
TAKTADYIWFNGEMVPWAEANVHVLTHAMHYGTSVFEGVRCYNTPKGPVIFRHPEHARR
CCCCCEEEEECCEECCCCCCCHHHHHHHHHHCHHHHHCHHCCCCCCCCEEEECCHHHHH
LKDSAKIYRFPIPYTEEEIMEATRETLRQNKLESAYIRPLGFVGNVGLGVCPPENTEMDL
HHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEE
IIAAFPWGSYLGEEALENGVDAMISSWNRAAPNTIPTAAKAGGNYLSSLLVGGEARRHGY
EEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHCCCHHHCCC
DEGIALSVDGYLSEGAGENIFVIRNGVLSTPPATSAILPGITRDSIMTLAKDMGYEIREE
CCCEEEEECCCCCCCCCCEEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCHHHHH
NIAREALYLADEVFMTGTAAEIVPVRSVDKITVGEGKRGPITEKVQAAYFGLFNGTTEDK
HHHHHHHHHHHHHHCCCCCHHEEECCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCC
WGWLDYVYPEHQTSANN
CCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]