Click here to switch to the map view.

The map label for this gene is plsB

Identifier: 218710907

GI number: 218710907

Start: 3158547

End: 3160970

Strand: Direct

Name: plsB

Synonym: VS_3009

Alternate gene names: 218710907

Gene position: 3158547-3160970 (Clockwise)

Preceding gene: 218710901

Following gene: 218710912

Centisome position: 95.73

GC content: 44.8

Gene sequence:

>2424_bases
ATGTCTTCTGGACAATCTTTTTCACGTTCATTAATGAAGCTACCTTTATCCCTATTGGTAAAGAGCACATCAATCCCTTC
AAACCCTGTAGAGGATTTGAACATTGATTTGAGCAAACCGATTGTATACGCCTTACCGTTTCGCTCAAGTGTAGACATTC
TTACATTGCAAAAACATGCACTTGAACTAGGGTTGCCTGATCCATTGAGCAAACTTGAAATCAATGGCAAATCACTGCAA
CGCTACGTTTTTATCTCTTCTCGCAAAACTCTACTGCAAGACGATGATTATGTACCAAGCTCATCAATTGAAGTCTTCTC
TGAACTGCTTTCACTGCATGCAGAAGACTCTGAGCTCGACGTTCAAGTTATCCCTGCCACCGTATTGTGGGGTCGAAAGC
CTGGTAAAGAGAATAACCAGAAGCCTTATCTACAAGCAATGAACGGCTTAGAGAAATCGAAAGCTGTATTACTAGCAGGT
CGTGACTGTCTGGTTCGCTTTAGCCCAGTGGTATCTCTACGATACATGGCTAACTCACACGGCACCGACAGCACCATCGC
GCATAAACTGGCGCGCGTGGCACGCATTCACTTCTCTCGTCAAAAACTTGCAGCATCGGGCCCTAACCTACCAAGCCGCC
AAGCTCTGTTCGACCGCCTGCTAAAGTCAGAAGCAATCAAAAAGGCGATAGAAGATGAAGCTGAATCAAAAAATATTTCC
ATAGAGAAAGCGAGCAAAGAAGCTCAAGACATCATGGACGAGATTGCTGCGAACTTCTCTTACTCGCTAATCAAACGCGG
TGAAAAGATTCTTGGTTGGTTGTGGAATAAGTTGTACCAAGGCCTGCATATCAGCAACGCTTCAACCGTTCGTAAGCTGG
CTCAAGATGGTCACGAGATCGTTTATGTACCTTGTCATCGTAGTCACATGGATTACTTACTGCTTTCTTATGTGCTTTAT
CATGAAGGCATGGTGCCTCCGCACATCGCAGCAGGGATCAACCTCAACTTCTTCCCTGCCGGCCCTATTTTCCGTCACGG
CGGTGCGTTCTTTATTCGTCGTAGTTTCAAAGGCAACAAGCTTTACTCAACGATTTTCCGTGAATACCTAGCCGAACTGT
TCGCTAAAGGTTACTCAGTAGAATACTTCAGTGAAGGTGGTCGTTCTCGTACGGGTCGTCTATTGCAGGCGAAAACCGGC
ATGCTAGCGATGACAATCCAAGCAATGCTGCGCGGCATGAACCGTCCGGTAACTTTGGTTCCTGTATACATCGGCTATGA
GCACGTAATGGAAGTCGCGACCTACGCGAAAGAACTTCGTGGTAAGCGTAAAGAGAAAGAAAACGCGAGCCTAGTGATTC
GTACAATTCGTAAGCTGCGTAACTTTGGTAAAGGCTACGTGAACTTCGGTGAGCCAATTCAGCTTAACCAATATCTGAAC
GAACATGCTCCTGAGTGGACTAAAGATATCGACCCAATGGGAACCAGCAAACCACAATGGATGAACCCGGTGGTTAATGA
CTTGGCAACCAAAATGATGACGCACATTAACGATGCGGCAGCGACCAATGCCCTGACTCTTTGTGCAACGGCACTATTGG
CTTCAAGACAGCGCGCATTGTCTCGAGACTCTTTGGTTTCTCAGATCAACTGCTACCTGTCTCTACTTAAGAATGTGCCT
TATTCAGATACATTCACAGTGCCTAAAGACAGCGCTGAAGACTTAGTAAAACACGCAGAGTCATTGAATAAGTTCTTAAT
TGAATCAGACACTATGGGCGACATCATTTCACTTGATCGTCACCAATCGATTCTGATGACCTACTATCGTAATAACATCA
TTCACTTGTTTGCATTGCCATCATTGATCGCTCAGATGACCATTCGTCAGCACGGTTTATCAATCGACGCTATTCAAGAA
AATGTTGCAGCTATCTACCCATTCTTAAAGAAAGAGTTGTTTTTAAGCTACGACGAAGATCAGCTTGAAAGCGTGGTTGC
GAACATCATTGATGAGCTAGTCGGTCAAGGCATGCTTGTTGTGTCGAACAACCAAGTAACGATCAACCAATCAAACAGCC
AAGCACTGATGCTACTAGGTCGTACGATTTCAGAGACTTTACAGCGTTACTCAATTGCGCTGAATCTATTGGCAGAGAAC
CCTGACCTTGATAAATCTGATCTTGAGCAGAAGAGCCAAGACATTGCACAACGACTGGGTCGTTTACACGGTATCAATGC
GCCAGAGTTCTTCGACAAAGGTGTATTCGCATCGATGTTTGCAACACTGAAACAGCAGCAATATCTGGATAACGATGGTA
ACTGTGATTTAGAAAAGACTCAGCAATTCGCTAAACTTCTCTACTCAATGCTTTACCCTGAAGTACGTTTGACGATTCAA
GAGAGTATCCACCAAGCAGAATAA

Upstream 100 bases:

>100_bases
CTAGGCTTATCCCTATTTCACTCATAGGATTTATCCTCTCATTATAGAAATAGAGATACGCTTGAGCACATTGAAATAAC
TATTGAGGCAGTGAACCTAT

Downstream 100 bases:

>100_bases
TCTCTTGGTTAGCATTCACTTTAATCACTAGACTGAACACTGACCCGTCCTGATATGGGTTGACACTTGATTGACTAAAA
CGCTCGATGTACTTCATCGG

Product: glycerol-3-phosphate acyltransferase

Products: NA

Alternate protein names: GPAT

Number of amino acids: Translated: 807; Mature: 806

Protein sequence:

>807_residues
MSSGQSFSRSLMKLPLSLLVKSTSIPSNPVEDLNIDLSKPIVYALPFRSSVDILTLQKHALELGLPDPLSKLEINGKSLQ
RYVFISSRKTLLQDDDYVPSSSIEVFSELLSLHAEDSELDVQVIPATVLWGRKPGKENNQKPYLQAMNGLEKSKAVLLAG
RDCLVRFSPVVSLRYMANSHGTDSTIAHKLARVARIHFSRQKLAASGPNLPSRQALFDRLLKSEAIKKAIEDEAESKNIS
IEKASKEAQDIMDEIAANFSYSLIKRGEKILGWLWNKLYQGLHISNASTVRKLAQDGHEIVYVPCHRSHMDYLLLSYVLY
HEGMVPPHIAAGINLNFFPAGPIFRHGGAFFIRRSFKGNKLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLQAKTG
MLAMTIQAMLRGMNRPVTLVPVYIGYEHVMEVATYAKELRGKRKEKENASLVIRTIRKLRNFGKGYVNFGEPIQLNQYLN
EHAPEWTKDIDPMGTSKPQWMNPVVNDLATKMMTHINDAAATNALTLCATALLASRQRALSRDSLVSQINCYLSLLKNVP
YSDTFTVPKDSAEDLVKHAESLNKFLIESDTMGDIISLDRHQSILMTYYRNNIIHLFALPSLIAQMTIRQHGLSIDAIQE
NVAAIYPFLKKELFLSYDEDQLESVVANIIDELVGQGMLVVSNNQVTINQSNSQALMLLGRTISETLQRYSIALNLLAEN
PDLDKSDLEQKSQDIAQRLGRLHGINAPEFFDKGVFASMFATLKQQQYLDNDGNCDLEKTQQFAKLLYSMLYPEVRLTIQ
ESIHQAE

Sequences:

>Translated_807_residues
MSSGQSFSRSLMKLPLSLLVKSTSIPSNPVEDLNIDLSKPIVYALPFRSSVDILTLQKHALELGLPDPLSKLEINGKSLQ
RYVFISSRKTLLQDDDYVPSSSIEVFSELLSLHAEDSELDVQVIPATVLWGRKPGKENNQKPYLQAMNGLEKSKAVLLAG
RDCLVRFSPVVSLRYMANSHGTDSTIAHKLARVARIHFSRQKLAASGPNLPSRQALFDRLLKSEAIKKAIEDEAESKNIS
IEKASKEAQDIMDEIAANFSYSLIKRGEKILGWLWNKLYQGLHISNASTVRKLAQDGHEIVYVPCHRSHMDYLLLSYVLY
HEGMVPPHIAAGINLNFFPAGPIFRHGGAFFIRRSFKGNKLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLQAKTG
MLAMTIQAMLRGMNRPVTLVPVYIGYEHVMEVATYAKELRGKRKEKENASLVIRTIRKLRNFGKGYVNFGEPIQLNQYLN
EHAPEWTKDIDPMGTSKPQWMNPVVNDLATKMMTHINDAAATNALTLCATALLASRQRALSRDSLVSQINCYLSLLKNVP
YSDTFTVPKDSAEDLVKHAESLNKFLIESDTMGDIISLDRHQSILMTYYRNNIIHLFALPSLIAQMTIRQHGLSIDAIQE
NVAAIYPFLKKELFLSYDEDQLESVVANIIDELVGQGMLVVSNNQVTINQSNSQALMLLGRTISETLQRYSIALNLLAEN
PDLDKSDLEQKSQDIAQRLGRLHGINAPEFFDKGVFASMFATLKQQQYLDNDGNCDLEKTQQFAKLLYSMLYPEVRLTIQ
ESIHQAE
>Mature_806_residues
SSGQSFSRSLMKLPLSLLVKSTSIPSNPVEDLNIDLSKPIVYALPFRSSVDILTLQKHALELGLPDPLSKLEINGKSLQR
YVFISSRKTLLQDDDYVPSSSIEVFSELLSLHAEDSELDVQVIPATVLWGRKPGKENNQKPYLQAMNGLEKSKAVLLAGR
DCLVRFSPVVSLRYMANSHGTDSTIAHKLARVARIHFSRQKLAASGPNLPSRQALFDRLLKSEAIKKAIEDEAESKNISI
EKASKEAQDIMDEIAANFSYSLIKRGEKILGWLWNKLYQGLHISNASTVRKLAQDGHEIVYVPCHRSHMDYLLLSYVLYH
EGMVPPHIAAGINLNFFPAGPIFRHGGAFFIRRSFKGNKLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLQAKTGM
LAMTIQAMLRGMNRPVTLVPVYIGYEHVMEVATYAKELRGKRKEKENASLVIRTIRKLRNFGKGYVNFGEPIQLNQYLNE
HAPEWTKDIDPMGTSKPQWMNPVVNDLATKMMTHINDAAATNALTLCATALLASRQRALSRDSLVSQINCYLSLLKNVPY
SDTFTVPKDSAEDLVKHAESLNKFLIESDTMGDIISLDRHQSILMTYYRNNIIHLFALPSLIAQMTIRQHGLSIDAIQEN
VAAIYPFLKKELFLSYDEDQLESVVANIIDELVGQGMLVVSNNQVTINQSNSQALMLLGRTISETLQRYSIALNLLAENP
DLDKSDLEQKSQDIAQRLGRLHGINAPEFFDKGVFASMFATLKQQQYLDNDGNCDLEKTQQFAKLLYSMLYPEVRLTIQE
SIHQAE

Specific function: De novo phospholipid biosynthesis; first step. [C]

COG id: COG2937

COG function: function code I; Glycerol-3-phosphate O-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GPAT/DAPAT family

Homologues:

Organism=Homo sapiens, GI7657134, Length=457, Percent_Identity=28.2275711159737, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI190358539, Length=425, Percent_Identity=24.9411764705882, Blast_Score=122, Evalue=1e-27,
Organism=Escherichia coli, GI87082362, Length=809, Percent_Identity=56.6131025957973, Blast_Score=946, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71988723, Length=493, Percent_Identity=27.3833671399594, Blast_Score=146, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI25147672, Length=281, Percent_Identity=30.2491103202847, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71988728, Length=416, Percent_Identity=28.6057692307692, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI17864692, Length=272, Percent_Identity=33.4558823529412, Blast_Score=160, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24650754, Length=483, Percent_Identity=25.0517598343685, Blast_Score=125, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21357731, Length=483, Percent_Identity=25.0517598343685, Blast_Score=125, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24650752, Length=483, Percent_Identity=25.0517598343685, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PLSB_VIBSL (B7VM97)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002418528.1
- GeneID:   7162474
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_3009
- HOGENOM:   HBG296590
- OMA:   VIYVPCH
- ProtClustDB:   PRK04974
- HAMAP:   MF_00393
- InterPro:   IPR002123
- InterPro:   IPR022284
- SMART:   SM00563
- TIGRFAMs:   TIGR03703

Pfam domain/function: PF01553 Acyltransferase

EC number: =2.3.1.15

Molecular weight: Translated: 90760; Mature: 90628

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSGQSFSRSLMKLPLSLLVKSTSIPSNPVEDLNIDLSKPIVYALPFRSSVDILTLQKHA
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHH
LELGLPDPLSKLEINGKSLQRYVFISSRKTLLQDDDYVPSSSIEVFSELLSLHAEDSELD
HHCCCCCCHHHHCCCCHHHEEEEEEECCCHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCE
VQVIPATVLWGRKPGKENNQKPYLQAMNGLEKSKAVLLAGRDCLVRFSPVVSLRYMANSH
EEEECHHEEECCCCCCCCCCCHHHHHHCCCCCCCEEEEECCHHHEEHHHHHHHHHHHCCC
GTDSTIAHKLARVARIHFSRQKLAASGPNLPSRQALFDRLLKSEAIKKAIEDEAESKNIS
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IEKASKEAQDIMDEIAANFSYSLIKRGEKILGWLWNKLYQGLHISNASTVRKLAQDGHEI
HHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEE
VYVPCHRSHMDYLLLSYVLYHEGMVPPHIAAGINLNFFPAGPIFRHGGAFFIRRSFKGNK
EEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCCCHHHCCCEEEEEECCCCCH
LYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLQAKTGMLAMTIQAMLRGMNRPVTLV
HHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCCEEEE
PVYIGYEHVMEVATYAKELRGKRKEKENASLVIRTIRKLRNFGKGYVNFGEPIQLNQYLN
EEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHH
EHAPEWTKDIDPMGTSKPQWMNPVVNDLATKMMTHINDAAATNALTLCATALLASRQRAL
HCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRDSLVSQINCYLSLLKNVPYSDTFTVPKDSAEDLVKHAESLNKFLIESDTMGDIISLDR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
HQSILMTYYRNNIIHLFALPSLIAQMTIRQHGLSIDAIQENVAAIYPFLKKELFLSYDED
HHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHH
QLESVVANIIDELVGQGMLVVSNNQVTINQSNSQALMLLGRTISETLQRYSIALNLLAEN
HHHHHHHHHHHHHHCCCEEEEECCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHEECC
PDLDKSDLEQKSQDIAQRLGRLHGINAPEFFDKGVFASMFATLKQQQYLDNDGNCDLEKT
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
QQFAKLLYSMLYPEVRLTIQESIHQAE
HHHHHHHHHHHCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSGQSFSRSLMKLPLSLLVKSTSIPSNPVEDLNIDLSKPIVYALPFRSSVDILTLQKHA
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHH
LELGLPDPLSKLEINGKSLQRYVFISSRKTLLQDDDYVPSSSIEVFSELLSLHAEDSELD
HHCCCCCCHHHHCCCCHHHEEEEEEECCCHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCE
VQVIPATVLWGRKPGKENNQKPYLQAMNGLEKSKAVLLAGRDCLVRFSPVVSLRYMANSH
EEEECHHEEECCCCCCCCCCCHHHHHHCCCCCCCEEEEECCHHHEEHHHHHHHHHHHCCC
GTDSTIAHKLARVARIHFSRQKLAASGPNLPSRQALFDRLLKSEAIKKAIEDEAESKNIS
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IEKASKEAQDIMDEIAANFSYSLIKRGEKILGWLWNKLYQGLHISNASTVRKLAQDGHEI
HHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEE
VYVPCHRSHMDYLLLSYVLYHEGMVPPHIAAGINLNFFPAGPIFRHGGAFFIRRSFKGNK
EEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCCCHHHCCCEEEEEECCCCCH
LYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLQAKTGMLAMTIQAMLRGMNRPVTLV
HHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCCEEEE
PVYIGYEHVMEVATYAKELRGKRKEKENASLVIRTIRKLRNFGKGYVNFGEPIQLNQYLN
EEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHH
EHAPEWTKDIDPMGTSKPQWMNPVVNDLATKMMTHINDAAATNALTLCATALLASRQRAL
HCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRDSLVSQINCYLSLLKNVPYSDTFTVPKDSAEDLVKHAESLNKFLIESDTMGDIISLDR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
HQSILMTYYRNNIIHLFALPSLIAQMTIRQHGLSIDAIQENVAAIYPFLKKELFLSYDED
HHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHH
QLESVVANIIDELVGQGMLVVSNNQVTINQSNSQALMLLGRTISETLQRYSIALNLLAEN
HHHHHHHHHHHHHHCCCEEEEECCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHEECC
PDLDKSDLEQKSQDIAQRLGRLHGINAPEFFDKGVFASMFATLKQQQYLDNDGNCDLEKT
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
QQFAKLLYSMLYPEVRLTIQESIHQAE
HHHHHHHHHHHCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA