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The map label for this gene is btuB [H]

Identifier: 218710897

GI number: 218710897

Start: 3148583

End: 3150343

Strand: Reverse

Name: btuB [H]

Synonym: VS_2998

Alternate gene names: 218710897

Gene position: 3150343-3148583 (Counterclockwise)

Preceding gene: 218710899

Following gene: 218710896

Centisome position: 95.49

GC content: 41.91

Gene sequence:

>1761_bases
ATGAACAAATCCCTTTTAGCGGTTGCTGTAGCATCGCTGCTTTCACCTATCTCCAATTTACACGCACAAGAAGCATCTGC
TGACGAAACCATGGTCGTTACGGCTAACCGTTTTGAGCAAAGCACTCAAAGTACAACGTTACCTGTTGTAGTTGTTACTC
GAGCAGAGATTGAAGCGATTCAAGCTAATGATATTTACGAAGTACTACGTCGTTTACCTGGTATTCAGGTAGCGAGCAAC
GGCGGCTACGGACAAAAGCAGAGTGTTTTTGTTAGAGGTACTAACTCTGACCACATCTTAGTACTGATTGATGGCGTAAA
AACAGCAAGTGCAACATCAGGAGGAGCAAATTTTGCTGCTATTCCACTAGTTGGTGTTCAGCGCATTGAGTTTGTGCGTG
GTCCTCGAGCTGCAATTTATGGAGCCGATGCGATTGGTGGTGTAATTAATATTATTACAGACACATCAAGTTCAGAAGCA
AAAGTTAGCGTAGGGTTTGGATCTGATTCATACAAAAAAGGCAGTGTGGCAGTGTCTGGTAATATTACAGACTCGACAAG
TGGTTCTGTAGCTGTAAAGCGAGCAGAGACTGATGGGTTTTCTGTTCAAAACTCAGCAGGTGATCAAGACGCAGATGGAC
ATGAAGTGACTGAGCTAGCACTTGCGATAACTCATAAGCTGAATGACGAGTTTAAGCTTAAGTTTAATTCTACTTACTCG
GATGGTGAAACAGAGTACGATCCGAAAAATGCTACAAAAGATCAGACACTGTACAGTGCAATTGGCCGTCTTAGTTATAC
TGGAGAAACGTTGTCGAGCAACTTTTCTTTAAGTAAAGCTCAAGATTCTAGTAAAGATTATTACTACAAAAGTACTTATC
AAACGGACCACCAGGCCGTTGCTTTCACAAATTTATTCACTGTGAACAATGAAGTTTCTATTGGTGCGGGCCTTGATTGG
TCTAAAGATGACGTATCAGAGTCCTCAAAAGACTATGCCGAGAAATCTCGCACGAATAACTCGCTATATGTTAGTGGCTT
TTATGACAACCAAGCCGTACAAGCTGAATTAGCTTTACGCGGTGACGATAACCAACGCTACGGCAAGAATACTACATGGC
AAGCGGGTTTAGGATATTGGTTAGCAGCTAATTATCGCGTGACAGCTAATGCTGGTACGGCATTTAAAGCACCGACATTT
AATCAACTATATTGGCCGGGCTCAGGTGTCCCAACGCTGAAACCTGAAGAGTCAAAAAACTATGAAGTCGCGTTCGAAGC
CCATCAATCATTGGTTGATTGGAGAGTATCGGCTTACAAAAATGATATTAAAAACATGATAGTTGGATGGCCATCAAAAA
ACATTGGTGTCGCTGAAATTAAAGGCTTAGAACTATCGGGAGAATTCGATACAGGAAGTTTTTCTCACCAAGTTAGCCTT
GACTTTATGGACCCGACAGATAAAAGCGATGGTTCTCAACTTTTACGTAGAGCGAAACAAAATGCTAAGTGGAACGTGAC
GTATTTTGCGGACAACTGGCAGGCGGACTTGAGTTATTTATATCAAGGTAAACGTATGGATAGCGGCAATGCTGAGTTAG
ATGAGTACTCGCTAGTAGATATAGCTGCGAGCTACTTTGTGACGGATCATTTCACAATGAGAGGGCGTATTGCTAACCTT
TTTGATAAAGATTACACGTTAGCCAAAGGGTATGAAACACAAGAAAGAAGTTTCTTTATAAACGCTGACTACCAGTTCTA
A

Upstream 100 bases:

>100_bases
TTAGGTAACCGAAAGGTTGTGCTCTTAAGTCCGAATACCTGCCAGCGGCTAAGCCATACAATGTGGTTTGGTAGGACTTT
ACGCGATTTAGGATCATAAC

Downstream 100 bases:

>100_bases
TTAACAACTAAACCGCCTTCGGGCGGTTTTTTCTTATTGGATATATCAATGAAAAAGAAAGTCGTCATAAGTTGGTCGTC
AGGTAAAGACTCTACGTTAA

Product: outer membrane protein

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MNKSLLAVAVASLLSPISNLHAQEASADETMVVTANRFEQSTQSTTLPVVVVTRAEIEAIQANDIYEVLRRLPGIQVASN
GGYGQKQSVFVRGTNSDHILVLIDGVKTASATSGGANFAAIPLVGVQRIEFVRGPRAAIYGADAIGGVINIITDTSSSEA
KVSVGFGSDSYKKGSVAVSGNITDSTSGSVAVKRAETDGFSVQNSAGDQDADGHEVTELALAITHKLNDEFKLKFNSTYS
DGETEYDPKNATKDQTLYSAIGRLSYTGETLSSNFSLSKAQDSSKDYYYKSTYQTDHQAVAFTNLFTVNNEVSIGAGLDW
SKDDVSESSKDYAEKSRTNNSLYVSGFYDNQAVQAELALRGDDNQRYGKNTTWQAGLGYWLAANYRVTANAGTAFKAPTF
NQLYWPGSGVPTLKPEESKNYEVAFEAHQSLVDWRVSAYKNDIKNMIVGWPSKNIGVAEIKGLELSGEFDTGSFSHQVSL
DFMDPTDKSDGSQLLRRAKQNAKWNVTYFADNWQADLSYLYQGKRMDSGNAELDEYSLVDIAASYFVTDHFTMRGRIANL
FDKDYTLAKGYETQERSFFINADYQF

Sequences:

>Translated_586_residues
MNKSLLAVAVASLLSPISNLHAQEASADETMVVTANRFEQSTQSTTLPVVVVTRAEIEAIQANDIYEVLRRLPGIQVASN
GGYGQKQSVFVRGTNSDHILVLIDGVKTASATSGGANFAAIPLVGVQRIEFVRGPRAAIYGADAIGGVINIITDTSSSEA
KVSVGFGSDSYKKGSVAVSGNITDSTSGSVAVKRAETDGFSVQNSAGDQDADGHEVTELALAITHKLNDEFKLKFNSTYS
DGETEYDPKNATKDQTLYSAIGRLSYTGETLSSNFSLSKAQDSSKDYYYKSTYQTDHQAVAFTNLFTVNNEVSIGAGLDW
SKDDVSESSKDYAEKSRTNNSLYVSGFYDNQAVQAELALRGDDNQRYGKNTTWQAGLGYWLAANYRVTANAGTAFKAPTF
NQLYWPGSGVPTLKPEESKNYEVAFEAHQSLVDWRVSAYKNDIKNMIVGWPSKNIGVAEIKGLELSGEFDTGSFSHQVSL
DFMDPTDKSDGSQLLRRAKQNAKWNVTYFADNWQADLSYLYQGKRMDSGNAELDEYSLVDIAASYFVTDHFTMRGRIANL
FDKDYTLAKGYETQERSFFINADYQF
>Mature_586_residues
MNKSLLAVAVASLLSPISNLHAQEASADETMVVTANRFEQSTQSTTLPVVVVTRAEIEAIQANDIYEVLRRLPGIQVASN
GGYGQKQSVFVRGTNSDHILVLIDGVKTASATSGGANFAAIPLVGVQRIEFVRGPRAAIYGADAIGGVINIITDTSSSEA
KVSVGFGSDSYKKGSVAVSGNITDSTSGSVAVKRAETDGFSVQNSAGDQDADGHEVTELALAITHKLNDEFKLKFNSTYS
DGETEYDPKNATKDQTLYSAIGRLSYTGETLSSNFSLSKAQDSSKDYYYKSTYQTDHQAVAFTNLFTVNNEVSIGAGLDW
SKDDVSESSKDYAEKSRTNNSLYVSGFYDNQAVQAELALRGDDNQRYGKNTTWQAGLGYWLAANYRVTANAGTAFKAPTF
NQLYWPGSGVPTLKPEESKNYEVAFEAHQSLVDWRVSAYKNDIKNMIVGWPSKNIGVAEIKGLELSGEFDTGSFSHQVSL
DFMDPTDKSDGSQLLRRAKQNAKWNVTYFADNWQADLSYLYQGKRMDSGNAELDEYSLVDIAASYFVTDHFTMRGRIANL
FDKDYTLAKGYETQERSFFINADYQF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=618, Percent_Identity=37.7022653721683, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1788478, Length=667, Percent_Identity=25.9370314842579, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI1786798, Length=177, Percent_Identity=31.638418079096, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 64102; Mature: 64102

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSLLAVAVASLLSPISNLHAQEASADETMVVTANRFEQSTQSTTLPVVVVTRAEIEAI
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHCCCCCCCCCCEEEEEECEEEEE
QANDIYEVLRRLPGIQVASNGGYGQKQSVFVRGTNSDHILVLIDGVKTASATSGGANFAA
CCCHHHHHHHHCCCEEEECCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCEEE
IPLVGVQRIEFVRGPRAAIYGADAIGGVINIITDTSSSEAKVSVGFGSDSYKKGSVAVSG
EEECCHHHHHHHCCCCCEEECCHHHCCEEEEEECCCCCCEEEEEECCCCCCCCCCEEEEC
NITDSTSGSVAVKRAETDGFSVQNSAGDQDADGHEVTELALAITHKLNDEFKLKFNSTYS
CCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCC
DGETEYDPKNATKDQTLYSAIGRLSYTGETLSSNFSLSKAQDSSKDYYYKSTYQTDHQAV
CCCCCCCCCCCCCHHHHHHHHHCCEECCCHHCCCCEECCCCCCCCCEEEEECCCCCCCEE
AFTNLFTVNNEVSIGAGLDWSKDDVSESSKDYAEKSRTNNSLYVSGFYDNQAVQAELALR
EEEEEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEEEEE
GDDNQRYGKNTTWQAGLGYWLAANYRVTANAGTAFKAPTFNQLYWPGSGVPTLKPEESKN
CCCCCCCCCCCCEECCCCEEEEECEEEEECCCCEECCCCCCEEEECCCCCCCCCCCCCCC
YEVAFEAHQSLVDWRVSAYKNDIKNMIVGWPSKNIGVAEIKGLELSGEFDTGSFSHQVSL
EEEEEHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEEEEECCCCCCCCCCEEEEE
DFMDPTDKSDGSQLLRRAKQNAKWNVTYFADNWQADLSYLYQGKRMDSGNAELDEYSLVD
EECCCCCCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCHHHH
IAASYFVTDHFTMRGRIANLFDKDYTLAKGYETQERSFFINADYQF
HHHHHEEEECEEECCHHHHHHCCCCEEECCCCCCCCEEEEEECCCC
>Mature Secondary Structure
MNKSLLAVAVASLLSPISNLHAQEASADETMVVTANRFEQSTQSTTLPVVVVTRAEIEAI
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHCCCCCCCCCCEEEEEECEEEEE
QANDIYEVLRRLPGIQVASNGGYGQKQSVFVRGTNSDHILVLIDGVKTASATSGGANFAA
CCCHHHHHHHHCCCEEEECCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCEEE
IPLVGVQRIEFVRGPRAAIYGADAIGGVINIITDTSSSEAKVSVGFGSDSYKKGSVAVSG
EEECCHHHHHHHCCCCCEEECCHHHCCEEEEEECCCCCCEEEEEECCCCCCCCCCEEEEC
NITDSTSGSVAVKRAETDGFSVQNSAGDQDADGHEVTELALAITHKLNDEFKLKFNSTYS
CCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCC
DGETEYDPKNATKDQTLYSAIGRLSYTGETLSSNFSLSKAQDSSKDYYYKSTYQTDHQAV
CCCCCCCCCCCCCHHHHHHHHHCCEECCCHHCCCCEECCCCCCCCCEEEEECCCCCCCEE
AFTNLFTVNNEVSIGAGLDWSKDDVSESSKDYAEKSRTNNSLYVSGFYDNQAVQAELALR
EEEEEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEEEEE
GDDNQRYGKNTTWQAGLGYWLAANYRVTANAGTAFKAPTFNQLYWPGSGVPTLKPEESKN
CCCCCCCCCCCCEECCCCEEEEECEEEEECCCCEECCCCCCEEEECCCCCCCCCCCCCCC
YEVAFEAHQSLVDWRVSAYKNDIKNMIVGWPSKNIGVAEIKGLELSGEFDTGSFSHQVSL
EEEEEHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEEEEECCCCCCCCCCEEEEE
DFMDPTDKSDGSQLLRRAKQNAKWNVTYFADNWQADLSYLYQGKRMDSGNAELDEYSLVD
EECCCCCCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCHHHH
IAASYFVTDHFTMRGRIANLFDKDYTLAKGYETQERSFFINADYQF
HHHHHEEEECEEECCHHHHHHCCCCEEECCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA