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The map label for this gene is murI [H]

Identifier: 218710895

GI number: 218710895

Start: 3147002

End: 3147832

Strand: Reverse

Name: murI [H]

Synonym: VS_2996

Alternate gene names: 218710895

Gene position: 3147832-3147002 (Counterclockwise)

Preceding gene: 218710896

Following gene: 218710890

Centisome position: 95.41

GC content: 42.96

Gene sequence:

>831_bases
TTGCAACGGAACTTATTACAGGTAATGGTTGTGTCGGAAAGCATACAAAAAAACATATTGATCTTTGATTCTGGAGTTGG
CGGCCTGTCCGTATATAAAGAGATAAGCCAGTTACTACCGAATCATAACTATACTTATGTATTCGATAACGAGGCTTACC
CATACGGTGAGCTAGACCAGCAAGTCCTTATCCACAGAGTTCAAAGCATCGTCGCCAGTTTTGTGGCTAGCCACGCTATT
GATATTGTGGTGATAGCCTGCAATACAGCAAGTACAATTGTGTTGCCTACACTAAGGGCAAATAACCCAATCCCTATTGT
CGGGGTTGTTCCGGCAATCAAACCAGCATCCCTACTTGCCAATAAAGCCGTTGGTCTTATTGCGACACCCGCAACGATTA
CTCGTGAATACACACACGAGCTCATCAAAAACTTCTCTAGTAATAAAAGAGTTGAGCTTTTAGGTTCGACTCAGCTGGTG
GATATGGCCGAAGATAAACTAAGAGGTGAGGCGATTAACCTTGAAGAGCTACGGCAAATCCTTCAACCAATGATCAACAC
AATTGATGTGGCTGTTTTAGGGTGCACTCATTTTCCACTGATCAAATCAGAGATTCAGCAAGTGCTAGGTAAAAACGTGA
TGTTGATTGATTCAGGTAAGGCTATTGCTAAGCGGGTTCAAGATTTATTGGATTTAGAGAGCAGCACTGAAGAAGGGGGA
GTGCGTGAGGCTTTTTGTAGTGCACTTCCTAAAAAAGAAAGTGCACTAAATAGTATGCTGAAGCAGTTAGGGTTTAGCTC
AGCTCAGCTTCGTCCGTTTCTGGATGTTTAG

Upstream 100 bases:

>100_bases
GCTGGAAAATGTAGAGCAGGGTGAGCGCTTTGTGCATCAACGCTACCGAGCCTGCATTATGTAGAGTGATGATTGGCGAA
AACTAAGTGAAGGGGTATCA

Downstream 100 bases:

>100_bases
GATCGTTTGCAACGCGTACTTTTAAAGTACGTTGCATGTAATCTTTATCGTTAAGTTCTGAAATAGCATGATTCACATCA
TTACTTGCCATAACAACAAA

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MQRNLLQVMVVSESIQKNILIFDSGVGGLSVYKEISQLLPNHNYTYVFDNEAYPYGELDQQVLIHRVQSIVASFVASHAI
DIVVIACNTASTIVLPTLRANNPIPIVGVVPAIKPASLLANKAVGLIATPATITREYTHELIKNFSSNKRVELLGSTQLV
DMAEDKLRGEAINLEELRQILQPMINTIDVAVLGCTHFPLIKSEIQQVLGKNVMLIDSGKAIAKRVQDLLDLESSTEEGG
VREAFCSALPKKESALNSMLKQLGFSSAQLRPFLDV

Sequences:

>Translated_276_residues
MQRNLLQVMVVSESIQKNILIFDSGVGGLSVYKEISQLLPNHNYTYVFDNEAYPYGELDQQVLIHRVQSIVASFVASHAI
DIVVIACNTASTIVLPTLRANNPIPIVGVVPAIKPASLLANKAVGLIATPATITREYTHELIKNFSSNKRVELLGSTQLV
DMAEDKLRGEAINLEELRQILQPMINTIDVAVLGCTHFPLIKSEIQQVLGKNVMLIDSGKAIAKRVQDLLDLESSTEEGG
VREAFCSALPKKESALNSMLKQLGFSSAQLRPFLDV
>Mature_276_residues
MQRNLLQVMVVSESIQKNILIFDSGVGGLSVYKEISQLLPNHNYTYVFDNEAYPYGELDQQVLIHRVQSIVASFVASHAI
DIVVIACNTASTIVLPTLRANNPIPIVGVVPAIKPASLLANKAVGLIATPATITREYTHELIKNFSSNKRVELLGSTQLV
DMAEDKLRGEAINLEELRQILQPMINTIDVAVLGCTHFPLIKSEIQQVLGKNVMLIDSGKAIAKRVQDLLDLESSTEEGG
VREAFCSALPKKESALNSMLKQLGFSSAQLRPFLDV

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis [H]

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family [H]

Homologues:

Organism=Escherichia coli, GI87082355, Length=257, Percent_Identity=47.0817120622568, Blast_Score=226, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391 [H]

Pfam domain/function: PF01177 Asp_Glu_race [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 30206; Mature: 30206

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00923 ASP_GLU_RACEMASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRNLLQVMVVSESIQKNILIFDSGVGGLSVYKEISQLLPNHNYTYVFDNEAYPYGELDQ
CCCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHH
QVLIHRVQSIVASFVASHAIDIVVIACNTASTIVLPTLRANNPIPIVGVVPAIKPASLLA
HHHHHHHHHHHHHHHHHHCEEEEEEEECCCCEEEEEEECCCCCCCEEEECCCCCHHHHHH
NKAVGLIATPATITREYTHELIKNFSSNKRVELLGSTQLVDMAEDKLRGEAINLEELRQI
HCCCCEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHH
LQPMINTIDVAVLGCTHFPLIKSEIQQVLGKNVMLIDSGKAIAKRVQDLLDLESSTEEGG
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCCCCC
VREAFCSALPKKESALNSMLKQLGFSSAQLRPFLDV
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MQRNLLQVMVVSESIQKNILIFDSGVGGLSVYKEISQLLPNHNYTYVFDNEAYPYGELDQ
CCCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHH
QVLIHRVQSIVASFVASHAIDIVVIACNTASTIVLPTLRANNPIPIVGVVPAIKPASLLA
HHHHHHHHHHHHHHHHHHCEEEEEEEECCCCEEEEEEECCCCCCCEEEECCCCCHHHHHH
NKAVGLIATPATITREYTHELIKNFSSNKRVELLGSTQLVDMAEDKLRGEAINLEELRQI
HCCCCEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHH
LQPMINTIDVAVLGCTHFPLIKSEIQQVLGKNVMLIDSGKAIAKRVQDLLDLESSTEEGG
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCCCCC
VREAFCSALPKKESALNSMLKQLGFSSAQLRPFLDV
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]