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The map label for this gene is dam [H]

Identifier: 218710786

GI number: 218710786

Start: 3016540

End: 3017400

Strand: Reverse

Name: dam [H]

Synonym: VS_2869

Alternate gene names: 218710786

Gene position: 3017400-3016540 (Counterclockwise)

Preceding gene: 218710787

Following gene: 218710785

Centisome position: 91.46

GC content: 45.3

Gene sequence:

>861_bases
ATGAAAAAGCAGCGTGCCTTTCTAAAATGGGCTGGTGGTAAATATGGCCTAGTTGAAGACATCCAACGTCACCTGCCACC
TGCTCGTAAATTGGTAGAACCGTTTGTCGGTGCCGGATCAGTATTTCTGAATACTGATTTTGAACAGTACCTACTTGCAG
ATATTAATCCTGATCTTATCAACCTATACAATCTACTTAAAACCGATCCTGAAACCTATATCACTGAAGCGAAGCGTTGG
TTCTGTCCGGAGAATAACCGTAGAGAAGTGTTCTTAGATGTTCGCGCTGAGTTCAACGGTACAGACAACGTTATGTATCG
CTCGTTAGCTTTCTTGTACATGAACCGATTTGGCTTCAATGGCTTGTGTCGTTACAACAAGAAGGGCGGTTTTAACGTTC
CGTTTGGTTCTTATAAAAAACCTTATTTCCCTGAAGCCGAGCTAGAGTTTTTTGCTGAGAAAGCGAAAAAAGCCACCTTC
GTCTGTGAGGGATACCATGAAACCTTTAGCCGCGCGCGTAAAGGTTGCGTGGTTTACTGTGATCCTCCATACGCACCGCT
GTCGAACACGGCTAACTTTACCTCTTACGCAGGTAATGGTTTTAGCTTAGATGACCAAGCTGCACTGGCGGATGTCGCTG
AAAAAGCGGCGATGGAACGCGATATTCCTGTCTTGATTTCAAACCATGACACCACCTTAACGCGCCGTTTATACCATGGT
GCGGAACTGAACGTGGTCAAGGTTAAGCGCACCATTAGCCGTAATGGCGCAGGGCGCAATAAAGTTGATGAGTTACTGGC
GCTTTTCCATAAAGAGAAAAACCAGCAACACGCTTCAACAGAATTGTCGTCGTCAGAATAA

Upstream 100 bases:

>100_bases
AGTGCATCGAGAGATAGAACGTGCGAAATAACCCTGAGCTTAGCCTCAAAATATGTTACATTCCGCAGCCTTATTTTTGA
GTTGATAGATAGAGCAGTAA

Downstream 100 bases:

>100_bases
TCCTCGAACTCATTAAATTGAGTGACCCACGGGTACTTTCTTAAACTAATCTTTCGAACTGCTAGGATCTGCTTAGGTGC
TTAGGTAGAATTGCACACCA

Product: DNA adenine methylase

Products: NA

Alternate protein names: DNA adenine methyltransferase; Deoxyadenosyl-methyltransferase; M.VchADam [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKKQRAFLKWAGGKYGLVEDIQRHLPPARKLVEPFVGAGSVFLNTDFEQYLLADINPDLINLYNLLKTDPETYITEAKRW
FCPENNRREVFLDVRAEFNGTDNVMYRSLAFLYMNRFGFNGLCRYNKKGGFNVPFGSYKKPYFPEAELEFFAEKAKKATF
VCEGYHETFSRARKGCVVYCDPPYAPLSNTANFTSYAGNGFSLDDQAALADVAEKAAMERDIPVLISNHDTTLTRRLYHG
AELNVVKVKRTISRNGAGRNKVDELLALFHKEKNQQHASTELSSSE

Sequences:

>Translated_286_residues
MKKQRAFLKWAGGKYGLVEDIQRHLPPARKLVEPFVGAGSVFLNTDFEQYLLADINPDLINLYNLLKTDPETYITEAKRW
FCPENNRREVFLDVRAEFNGTDNVMYRSLAFLYMNRFGFNGLCRYNKKGGFNVPFGSYKKPYFPEAELEFFAEKAKKATF
VCEGYHETFSRARKGCVVYCDPPYAPLSNTANFTSYAGNGFSLDDQAALADVAEKAAMERDIPVLISNHDTTLTRRLYHG
AELNVVKVKRTISRNGAGRNKVDELLALFHKEKNQQHASTELSSSE
>Mature_286_residues
MKKQRAFLKWAGGKYGLVEDIQRHLPPARKLVEPFVGAGSVFLNTDFEQYLLADINPDLINLYNLLKTDPETYITEAKRW
FCPENNRREVFLDVRAEFNGTDNVMYRSLAFLYMNRFGFNGLCRYNKKGGFNVPFGSYKKPYFPEAELEFFAEKAKKATF
VCEGYHETFSRARKGCVVYCDPPYAPLSNTANFTSYAGNGFSLDDQAALADVAEKAAMERDIPVLISNHDTTLTRRLYHG
AELNVVKVKRTISRNGAGRNKVDELLALFHKEKNQQHASTELSSSE

Specific function: Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=269, Percent_Identity=63.1970260223048, Blast_Score=351, Evalue=3e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 32576; Mature: 32576

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQRAFLKWAGGKYGLVEDIQRHLPPARKLVEPFVGAGSVFLNTDFEQYLLADINPDLI
CCCCCCCEEECCCCCCHHHHHHHHCCHHHHHHHHHHCCCEEEEECCHHHHHHHCCCHHHH
NLYNLLKTDPETYITEAKRWFCPENNRREVFLDVRAEFNGTDNVMYRSLAFLYMNRFGFN
HHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCC
GLCRYNKKGGFNVPFGSYKKPYFPEAELEFFAEKAKKATFVCEGYHETFSRARKGCVVYC
CEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEECCHHHHHHHHHCCEEEEE
DPPYAPLSNTANFTSYAGNGFSLDDQAALADVAEKAAMERDIPVLISNHDTTLTRRLYHG
CCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCC
AELNVVKVKRTISRNGAGRNKVDELLALFHKEKNQQHASTELSSSE
CCCEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCC
>Mature Secondary Structure
MKKQRAFLKWAGGKYGLVEDIQRHLPPARKLVEPFVGAGSVFLNTDFEQYLLADINPDLI
CCCCCCCEEECCCCCCHHHHHHHHCCHHHHHHHHHHCCCEEEEECCHHHHHHHCCCHHHH
NLYNLLKTDPETYITEAKRWFCPENNRREVFLDVRAEFNGTDNVMYRSLAFLYMNRFGFN
HHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCC
GLCRYNKKGGFNVPFGSYKKPYFPEAELEFFAEKAKKATFVCEGYHETFSRARKGCVVYC
CEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEECCHHHHHHHHHCCEEEEE
DPPYAPLSNTANFTSYAGNGFSLDDQAALADVAEKAAMERDIPVLISNHDTTLTRRLYHG
CCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCC
AELNVVKVKRTISRNGAGRNKVDELLALFHKEKNQQHASTELSSSE
CCCEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11705940 [H]