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The map label for this gene is thiP [H]

Identifier: 218710686

GI number: 218710686

Start: 2928893

End: 2930506

Strand: Reverse

Name: thiP [H]

Synonym: VS_2766

Alternate gene names: 218710686

Gene position: 2930506-2928893 (Counterclockwise)

Preceding gene: 218710687

Following gene: 218710685

Centisome position: 88.82

GC content: 44.92

Gene sequence:

>1614_bases
ATGATAAAGAAAACACCTAAGGTCGGGGTTTGGGTTGCGATACTCATTACCGCCTTCGTGGTTTCAGCAGTTGGGGCATT
GCTTAGCAATGCCCCTTCTCTTGATATCAGCCAAGTATGGTCCGATCCTTACTATTGGCATGTAACGAAATTCAGTTTCT
ATCAAGCGACACTCTCAACGATGCTGAGTGTTGGCTTTGCTATACCTATTGCTCATGCTCTGTGTCGCAGACAATTTTTT
GGACGGGCCTTGTTGTTAAGGCTGTTTGCTTCGACCTTGGTTTTACCCGTATTAGTTGGCGTATTTGGTTTGCTAGCTAT
TTATGGTAACAGTGGTTGGTTAGCTAAATTTCTGGCGAACTTCGATATTAAACTGCCATTCTCGATTTATGGGTTGAATG
GTATTTTGCTCGCACATGTCTTCTTTAACCTGCCTTATGCTAGCCGTTTACTTTTACAAGCTTTAGATACCGTACCTGCC
GAGCAGCATAAGCTTTGTGCTCATTTGGGTATGAGTCATTGGAACAAATTCAGATGGGTTGAATGGCCTCGTTTAAGACA
GCAATTGCCACATGTGTGTGGTTTGGTCTTCATGCTGTGTTTTACCAGCTTTGCTACCGTGATGGCACTAGGCGGAGGAC
CTAAATCAACCACTATCGAACTCGCTATTTATCAGGCGATTAAATTCGACTTTGACCTGCAGGCTGGTGCCTTGCTCGCG
ATATGGCAAATGCTGCTGTGTGGTGTGCTAGCTGTGAGCATTCAACGCCTATCGAAACCTATTTCAGTAACAGCCAGTCA
ATTGTCGGAAGAGAAATATTTGGTTAAGGACAGCTGGTGGTCAAAAGCTTGGGATAGCTTTTGGATCATCACCGTCTCAA
TGCTGGTACTGCCGCCATTAGCTATGGTTATCGTCAGTGGCATTAACTCGCAGGCATTAATCGTACTGAGCAGCGAGCCG
TTTTGGGCTGCGTTAATGACCTCGGTTAAAGTCGCGTCATTAGCAAGTGTTATCGCGGTTGGTATTGGTATCGCAATACT
GCTGACGAGTCGAGCATGGCGCTTGCAGAATAAGAATTTTCGAGCAGATAAAATCGAGCTGATTGGCACTATTATCTTAG
TGACTCCCGGGCTTGTGATCAGTACGGGTCTCTTCTTATTACTGCGTTCATTCACCGATGTTTTCAGCTTAGCCTTCTTT
ATCGTGATTGCAGTAAACAGTTTAATGGCGCTTCCTTACGTAATTAAAACCTTGGCTCAACCTATGCTGCATTTGTCTCA
GCAGTATCAGTATGTGTGCGCAAGTTTGGGAATAAGTGGTTTCACGCGTTTTAGGCTAGTGGAGTGGCGCGCATTGCGTA
AACCGATGGCACAAGCCTTCTCGATCAGCTTTATGTTAGCAATGGGTGACTTAAGCGCGATTGCTTTGTTTGGTAGCCAA
GATTTTAGAACTCTGCCCTTGTACCTATTCCAATTATTAGGCAGCTATCAAATGGAAGCCGCAGCGGTGGTTTCCGTGAG
CTTACTGTTACTGAGTGTTGGCAGCTTTAGCCTGATTGAATTTTTATTTACTCGAAGATCTAAGCTCGATGCTAAAGAGT
TGAGGAACCGATGA

Upstream 100 bases:

>100_bases
AAAATGAGTTTTCTAACGTACTTTTAAAAAACTAGCTTTCTGTATTTAAAGGTTCATTCGTTATGTTTGGAGTGAACCAT
TTACGGTAAAAAATCAATTT

Downstream 100 bases:

>100_bases
TGTTAGTGATGAAAGATGTGGACTACCACTATCACAGAGAGTTGTTTTGCTTTGATTTTCAAGCTGAGCAGGGCGATATT
GTTGCGCTGATGGGGCCAAG

Product: thiamine transporter membrane protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MIKKTPKVGVWVAILITAFVVSAVGALLSNAPSLDISQVWSDPYYWHVTKFSFYQATLSTMLSVGFAIPIAHALCRRQFF
GRALLLRLFASTLVLPVLVGVFGLLAIYGNSGWLAKFLANFDIKLPFSIYGLNGILLAHVFFNLPYASRLLLQALDTVPA
EQHKLCAHLGMSHWNKFRWVEWPRLRQQLPHVCGLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQAGALLA
IWQMLLCGVLAVSIQRLSKPISVTASQLSEEKYLVKDSWWSKAWDSFWIITVSMLVLPPLAMVIVSGINSQALIVLSSEP
FWAALMTSVKVASLASVIAVGIGIAILLTSRAWRLQNKNFRADKIELIGTIILVTPGLVISTGLFLLLRSFTDVFSLAFF
IVIAVNSLMALPYVIKTLAQPMLHLSQQYQYVCASLGISGFTRFRLVEWRALRKPMAQAFSISFMLAMGDLSAIALFGSQ
DFRTLPLYLFQLLGSYQMEAAAVVSVSLLLLSVGSFSLIEFLFTRRSKLDAKELRNR

Sequences:

>Translated_537_residues
MIKKTPKVGVWVAILITAFVVSAVGALLSNAPSLDISQVWSDPYYWHVTKFSFYQATLSTMLSVGFAIPIAHALCRRQFF
GRALLLRLFASTLVLPVLVGVFGLLAIYGNSGWLAKFLANFDIKLPFSIYGLNGILLAHVFFNLPYASRLLLQALDTVPA
EQHKLCAHLGMSHWNKFRWVEWPRLRQQLPHVCGLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQAGALLA
IWQMLLCGVLAVSIQRLSKPISVTASQLSEEKYLVKDSWWSKAWDSFWIITVSMLVLPPLAMVIVSGINSQALIVLSSEP
FWAALMTSVKVASLASVIAVGIGIAILLTSRAWRLQNKNFRADKIELIGTIILVTPGLVISTGLFLLLRSFTDVFSLAFF
IVIAVNSLMALPYVIKTLAQPMLHLSQQYQYVCASLGISGFTRFRLVEWRALRKPMAQAFSISFMLAMGDLSAIALFGSQ
DFRTLPLYLFQLLGSYQMEAAAVVSVSLLLLSVGSFSLIEFLFTRRSKLDAKELRNR
>Mature_537_residues
MIKKTPKVGVWVAILITAFVVSAVGALLSNAPSLDISQVWSDPYYWHVTKFSFYQATLSTMLSVGFAIPIAHALCRRQFF
GRALLLRLFASTLVLPVLVGVFGLLAIYGNSGWLAKFLANFDIKLPFSIYGLNGILLAHVFFNLPYASRLLLQALDTVPA
EQHKLCAHLGMSHWNKFRWVEWPRLRQQLPHVCGLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQAGALLA
IWQMLLCGVLAVSIQRLSKPISVTASQLSEEKYLVKDSWWSKAWDSFWIITVSMLVLPPLAMVIVSGINSQALIVLSSEP
FWAALMTSVKVASLASVIAVGIGIAILLTSRAWRLQNKNFRADKIELIGTIILVTPGLVISTGLFLLLRSFTDVFSLAFF
IVIAVNSLMALPYVIKTLAQPMLHLSQQYQYVCASLGISGFTRFRLVEWRALRKPMAQAFSISFMLAMGDLSAIALFGSQ
DFRTLPLYLFQLLGSYQMEAAAVVSVSLLLLSVGSFSLIEFLFTRRSKLDAKELRNR

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1786254, Length=501, Percent_Identity=49.500998003992, Blast_Score=434, Evalue=1e-122,
Organism=Escherichia coli, GI1788764, Length=205, Percent_Identity=26.3414634146341, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 59576; Mature: 59576

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKTPKVGVWVAILITAFVVSAVGALLSNAPSLDISQVWSDPYYWHVTKFSFYQATLST
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEEEHHHHHHHHHHH
MLSVGFAIPIAHALCRRQFFGRALLLRLFASTLVLPVLVGVFGLLAIYGNSGWLAKFLAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHC
FDIKLPFSIYGLNGILLAHVFFNLPYASRLLLQALDTVPAEQHKLCAHLGMSHWNKFRWV
CCEECCEEEECHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCHHHCCCCCCC
EWPRLRQQLPHVCGLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQAGALLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCCCCCCHHHH
IWQMLLCGVLAVSIQRLSKPISVTASQLSEEKYLVKDSWWSKAWDSFWIITVSMLVLPPL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEHHCCHHHHHHCHHHHHHHHHHHHHHH
AMVIVSGINSQALIVLSSEPFWAALMTSVKVASLASVIAVGIGIAILLTSRAWRLQNKNF
HHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RADKIELIGTIILVTPGLVISTGLFLLLRSFTDVFSLAFFIVIAVNSLMALPYVIKTLAQ
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PMLHLSQQYQYVCASLGISGFTRFRLVEWRALRKPMAQAFSISFMLAMGDLSAIALFGSQ
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCC
DFRTLPLYLFQLLGSYQMEAAAVVSVSLLLLSVGSFSLIEFLFTRRSKLDAKELRNR
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHCCC
>Mature Secondary Structure
MIKKTPKVGVWVAILITAFVVSAVGALLSNAPSLDISQVWSDPYYWHVTKFSFYQATLST
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEEEHHHHHHHHHHH
MLSVGFAIPIAHALCRRQFFGRALLLRLFASTLVLPVLVGVFGLLAIYGNSGWLAKFLAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHC
FDIKLPFSIYGLNGILLAHVFFNLPYASRLLLQALDTVPAEQHKLCAHLGMSHWNKFRWV
CCEECCEEEECHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCHHHCCCCCCC
EWPRLRQQLPHVCGLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQAGALLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCCCCCCHHHH
IWQMLLCGVLAVSIQRLSKPISVTASQLSEEKYLVKDSWWSKAWDSFWIITVSMLVLPPL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEHHCCHHHHHHCHHHHHHHHHHHHHHH
AMVIVSGINSQALIVLSSEPFWAALMTSVKVASLASVIAVGIGIAILLTSRAWRLQNKNF
HHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RADKIELIGTIILVTPGLVISTGLFLLLRSFTDVFSLAFFIVIAVNSLMALPYVIKTLAQ
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PMLHLSQQYQYVCASLGISGFTRFRLVEWRALRKPMAQAFSISFMLAMGDLSAIALFGSQ
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCC
DFRTLPLYLFQLLGSYQMEAAAVVSVSLLLLSVGSFSLIEFLFTRRSKLDAKELRNR
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]