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The map label for this gene is ptsN [H]

Identifier: 218710679

GI number: 218710679

Start: 2922830

End: 2923288

Strand: Direct

Name: ptsN [H]

Synonym: VS_2759

Alternate gene names: 218710679

Gene position: 2922830-2923288 (Clockwise)

Preceding gene: 218710678

Following gene: 218710680

Centisome position: 88.59

GC content: 43.36

Gene sequence:

>459_bases
ATGCAATTAAGCGAAGTACTTTCATTGGACTGCACTAAAAGTGCAGTCCAATGCACAAGCAAAAAAAGAGCCCTTGAGCT
GATCAGTCAAATCGCAGCAGAAAGCTGCGGTCAAGATTCAACAGAACTGTTTGAGTGCATGCTGAGCCGTGAAAAAATGG
GCAGTACAGGTATCGGTAACGGTATCGCAATTCCCCATGCTCGCATGAATGTCAGTGATAAAGCGATTGCTGTATTAATA
CAATGCCAAGACCCAATCGAATTTGATGCTATCGATAACCGCCCCGTTGATCTACTGTTTGCTCTACTTGTTCCTAGTGA
ACAATGTAAAGAGCATCTGAAAACCCTTTCTTGTATGGCAGAGCGACTTAACGACAAGCAGACTCTTAAACAGTTGCGTA
ATGCTCAAAGCGATCAAGAGCTTTACGACATCATGATTCAAGTGCCAACTTGCGAGTAA

Upstream 100 bases:

>100_bases
ACAGCAACAGATGAAAGCATGTATGCCGCGATTGATTCATTGGTTGATAAACTTGTTCGCCAACTCAACAAGCACAAAGA
AAAACTAAGTAGTCACTAAC

Downstream 100 bases:

>100_bases
TAACATGCGATTAATCGTTGTTAGTGGCCAGTCAGGAGCTGGAAAAAGTGTTGCGCTACGAGTTCTTGAAGACTTGGGGT
ATTACTGTGTTGATAATCTA

Product: nitrogen regulatory protein

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MQLSEVLSLDCTKSAVQCTSKKRALELISQIAAESCGQDSTELFECMLSREKMGSTGIGNGIAIPHARMNVSDKAIAVLI
QCQDPIEFDAIDNRPVDLLFALLVPSEQCKEHLKTLSCMAERLNDKQTLKQLRNAQSDQELYDIMIQVPTCE

Sequences:

>Translated_152_residues
MQLSEVLSLDCTKSAVQCTSKKRALELISQIAAESCGQDSTELFECMLSREKMGSTGIGNGIAIPHARMNVSDKAIAVLI
QCQDPIEFDAIDNRPVDLLFALLVPSEQCKEHLKTLSCMAERLNDKQTLKQLRNAQSDQELYDIMIQVPTCE
>Mature_152_residues
MQLSEVLSLDCTKSAVQCTSKKRALELISQIAAESCGQDSTELFECMLSREKMGSTGIGNGIAIPHARMNVSDKAIAVLI
QCQDPIEFDAIDNRPVDLLFALLVPSEQCKEHLKTLSCMAERLNDKQTLKQLRNAQSDQELYDIMIQVPTCE

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=146, Percent_Identity=58.2191780821918, Blast_Score=175, Evalue=1e-45,
Organism=Escherichia coli, GI1786951, Length=144, Percent_Identity=27.0833333333333, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16848; Mature: 16848

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
9.2 %Cys+Met (Translated Protein)
5.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
9.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLSEVLSLDCTKSAVQCTSKKRALELISQIAAESCGQDSTELFECMLSREKMGSTGIGN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
GIAIPHARMNVSDKAIAVLIQCQDPIEFDAIDNRPVDLLFALLVPSEQCKEHLKTLSCMA
CEECCCCCCCCCHHHEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
ERLNDKQTLKQLRNAQSDQELYDIMIQVPTCE
HHCCCHHHHHHHHCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQLSEVLSLDCTKSAVQCTSKKRALELISQIAAESCGQDSTELFECMLSREKMGSTGIGN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
GIAIPHARMNVSDKAIAVLIQCQDPIEFDAIDNRPVDLLFALLVPSEQCKEHLKTLSCMA
CEECCCCCCCCCHHHEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
ERLNDKQTLKQLRNAQSDQELYDIMIQVPTCE
HHCCCHHHHHHHHCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2695747 [H]