Click here to switch to the map view.

The map label for this gene is rpoN [H]

Identifier: 218710677

GI number: 218710677

Start: 2920990

End: 2922450

Strand: Direct

Name: rpoN [H]

Synonym: VS_2757

Alternate gene names: 218710677

Gene position: 2920990-2922450 (Clockwise)

Preceding gene: 218710676

Following gene: 218710678

Centisome position: 88.53

GC content: 44.97

Gene sequence:

>1461_bases
ATGAAACCCTCATTACAACTTAAGCTAGGCCAACAGTTAGCAATGACTCCTCAATTGCAACAAGCGATTCGTTTGTTGCA
ATTGTCTACTTTGGATTTGCAACAAGAGATTCAAGAAGCACTTGAATCGAACCCACTACTCGATGTCGAAGACGGCAATG
AAGATACGCCAACATCGGAAGAAAAACCTAGCAGTGACGAGAAAGAAACAGTTGAAACAGTTGAACAAGACTTACCTGAC
AGCTCCGATTTAATCGAAAAATCAGAGATCGGCAACGAACTAGAAATCGATACAACTTGGGAAGACGTTTACAGCGCAAA
CACAGGTAACACTGGCATTGCGATTGATGACGATATGCCCGTTTATCAAGGCGAAACCACACAAAGTCTATACGATTACC
TGCTCTGGCAACTCGATTTAACACCATTTACTGAAACTGACCGCAGCATAGCTTTTGCGCTAATTGATGCTATCGATGAC
CGCGGTTACCTTACCGTATCTTGTGAAGACATCCTTGAGAACTTCGACAACGAAGAGATCGAGCTTGATGAAATTGAAGC
CGTTCGTAAACGAATTCAGCAGTTTGATCCGCTAGGTGTCGGTTCTGTGAATCTGCAAGATTGCTTGTTACTGCAATTGG
CGACCTTCCCACAAGATACCCCTTGGCTTAACGAAGCTAAGTTAGTACTTACTAGCCATATCGACCAACTCGGCAATCGC
GATTACAAACTGGTTATCAAAGAGACCAAGTTGAAAGAAGCGGAACTACGCGAAGTTTTGCAATTAATCCAACAGCTGGA
CCCTCGCCCTGGCAGTAAGATCACGCCAGACGAAACCGAGTATGTGGTTCCTGACGTATCCGTCTTCAAAGAGCTTGGGA
AATGGTTGGTCACCATCAATCCAGATAGCGTGCCAAAACTAAAAGTGAATCAACAATACGCGGCCCTTAGTAGTAAAGGC
AGTAGCGCTGACAACCAATTTATCCGCTCAAATTTGCAAGAAGCAAAATGGCTAATTAAAAGCCTAGAGAGCCGAAATGA
AACGCTACTAAAAGTCGCTCGGTGTATTGTTGAACATCAACGCGGTTTCTTCGAACATGGCGCAGAAGCCATGAAGCCGA
TGGTACTGAATGATGTGGCTTTAGCTGTTGATATGCACGAATCCACGATCTCTCGTGTGACGACTCAAAAATTCATGCAC
ACACCGCGTGGCATCTTCGAACTCAAGTACTTTTTCTCAAGCCATGTCAGCACTGACAATGGCGGTGAATGTTCATCTAC
AGCAATTCGCGCACTCATTAAGAAGCTTGTCGCAGCGGAAAATACCGCAAAGCCTCTCAGTGATAGTAAGATTGCTGCTT
TACTGGCTGACCAAGGAATTCAGGTAGCTAGACGTACCATAGCGAAATACCGTGAATCTCTGGGCATTGCCCCATCGAGT
CAGCGTAAACGCCTGCTATAA

Upstream 100 bases:

>100_bases
CGAACAAGTTAAACAAGTTTATCTAGGCGAACAATTCCGTCTATGATTAGATAGAGAGTATCGAGAGTTCTTAGAATAAT
AACTAGGTAAGTAACACTGA

Downstream 100 bases:

>100_bases
ACTAAAGTAGTTAGCTCTTTATTCATTAACGATTGATTAGTTAACTATTAATTTAGTTGTAACCTGGCAACCAACTGAAA
AGGAAAGTCTATGCAAATCA

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MKPSLQLKLGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALESNPLLDVEDGNEDTPTSEEKPSSDEKETVETVEQDLPD
SSDLIEKSEIGNELEIDTTWEDVYSANTGNTGIAIDDDMPVYQGETTQSLYDYLLWQLDLTPFTETDRSIAFALIDAIDD
RGYLTVSCEDILENFDNEEIELDEIEAVRKRIQQFDPLGVGSVNLQDCLLLQLATFPQDTPWLNEAKLVLTSHIDQLGNR
DYKLVIKETKLKEAELREVLQLIQQLDPRPGSKITPDETEYVVPDVSVFKELGKWLVTINPDSVPKLKVNQQYAALSSKG
SSADNQFIRSNLQEAKWLIKSLESRNETLLKVARCIVEHQRGFFEHGAEAMKPMVLNDVALAVDMHESTISRVTTQKFMH
TPRGIFELKYFFSSHVSTDNGGECSSTAIRALIKKLVAAENTAKPLSDSKIAALLADQGIQVARRTIAKYRESLGIAPSS
QRKRLL

Sequences:

>Translated_486_residues
MKPSLQLKLGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALESNPLLDVEDGNEDTPTSEEKPSSDEKETVETVEQDLPD
SSDLIEKSEIGNELEIDTTWEDVYSANTGNTGIAIDDDMPVYQGETTQSLYDYLLWQLDLTPFTETDRSIAFALIDAIDD
RGYLTVSCEDILENFDNEEIELDEIEAVRKRIQQFDPLGVGSVNLQDCLLLQLATFPQDTPWLNEAKLVLTSHIDQLGNR
DYKLVIKETKLKEAELREVLQLIQQLDPRPGSKITPDETEYVVPDVSVFKELGKWLVTINPDSVPKLKVNQQYAALSSKG
SSADNQFIRSNLQEAKWLIKSLESRNETLLKVARCIVEHQRGFFEHGAEAMKPMVLNDVALAVDMHESTISRVTTQKFMH
TPRGIFELKYFFSSHVSTDNGGECSSTAIRALIKKLVAAENTAKPLSDSKIAALLADQGIQVARRTIAKYRESLGIAPSS
QRKRLL
>Mature_486_residues
MKPSLQLKLGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALESNPLLDVEDGNEDTPTSEEKPSSDEKETVETVEQDLPD
SSDLIEKSEIGNELEIDTTWEDVYSANTGNTGIAIDDDMPVYQGETTQSLYDYLLWQLDLTPFTETDRSIAFALIDAIDD
RGYLTVSCEDILENFDNEEIELDEIEAVRKRIQQFDPLGVGSVNLQDCLLLQLATFPQDTPWLNEAKLVLTSHIDQLGNR
DYKLVIKETKLKEAELREVLQLIQQLDPRPGSKITPDETEYVVPDVSVFKELGKWLVTINPDSVPKLKVNQQYAALSSKG
SSADNQFIRSNLQEAKWLIKSLESRNETLLKVARCIVEHQRGFFEHGAEAMKPMVLNDVALAVDMHESTISRVTTQKFMH
TPRGIFELKYFFSSHVSTDNGGECSSTAIRALIKKLVAAENTAKPLSDSKIAALLADQGIQVARRTIAKYRESLGIAPSS
QRKRLL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=489, Percent_Identity=61.1451942740286, Blast_Score=622, Evalue=1e-179,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 54568; Mature: 54568

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSLQLKLGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALESNPLLDVEDGNEDTPTSE
CCCCCEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCC
EKPSSDEKETVETVEQDLPDSSDLIEKSEIGNELEIDTTWEDVYSANTGNTGIAIDDDMP
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCEEEECCCHHHHHCCCCCCCCEEECCCCC
VYQGETTQSLYDYLLWQLDLTPFTETDRSIAFALIDAIDDRGYLTVSCEDILENFDNEEI
CCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCCC
ELDEIEAVRKRIQQFDPLGVGSVNLQDCLLLQLATFPQDTPWLNEAKLVLTSHIDQLGNR
CHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC
DYKLVIKETKLKEAELREVLQLIQQLDPRPGSKITPDETEYVVPDVSVFKELGKWLVTIN
CEEEEEEHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCHHHHHHHCCEEEEEC
PDSVPKLKVNQQYAALSSKGSSADNQFIRSNLQEAKWLIKSLESRNETLLKVARCIVEHQ
CCCCCCEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RGFFEHGAEAMKPMVLNDVALAVDMHESTISRVTTQKFMHTPRGIFELKYFFSSHVSTDN
HHHHHHHHHHHCCHHHHHHHHEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
GGECSSTAIRALIKKLVAAENTAKPLSDSKIAALLADQGIQVARRTIAKYRESLGIAPSS
CCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCC
QRKRLL
CCCCCC
>Mature Secondary Structure
MKPSLQLKLGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALESNPLLDVEDGNEDTPTSE
CCCCCEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCC
EKPSSDEKETVETVEQDLPDSSDLIEKSEIGNELEIDTTWEDVYSANTGNTGIAIDDDMP
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCEEEECCCHHHHHCCCCCCCCEEECCCCC
VYQGETTQSLYDYLLWQLDLTPFTETDRSIAFALIDAIDDRGYLTVSCEDILENFDNEEI
CCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCCC
ELDEIEAVRKRIQQFDPLGVGSVNLQDCLLLQLATFPQDTPWLNEAKLVLTSHIDQLGNR
CHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC
DYKLVIKETKLKEAELREVLQLIQQLDPRPGSKITPDETEYVVPDVSVFKELGKWLVTIN
CEEEEEEHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCHHHHHHHCCEEEEEC
PDSVPKLKVNQQYAALSSKGSSADNQFIRSNLQEAKWLIKSLESRNETLLKVARCIVEHQ
CCCCCCEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RGFFEHGAEAMKPMVLNDVALAVDMHESTISRVTTQKFMHTPRGIFELKYFFSSHVSTDN
HHHHHHHHHHHCCHHHHHHHHEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
GGECSSTAIRALIKKLVAAENTAKPLSDSKIAALLADQGIQVARRTIAKYRESLGIAPSS
CCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCC
QRKRLL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9421909 [H]