Click here to switch to the map view.

The map label for this gene is trmB [H]

Identifier: 218710637

GI number: 218710637

Start: 2877423

End: 2878160

Strand: Reverse

Name: trmB [H]

Synonym: VS_2691

Alternate gene names: 218710637

Gene position: 2878160-2877423 (Counterclockwise)

Preceding gene: 218710641

Following gene: 218710636

Centisome position: 87.24

GC content: 46.07

Gene sequence:

>738_bases
ATGTGCAGGCAAAAATCAATGAGTGAAGTGACCACGAACGAATATACTGAAGACGGCAAACTGGTTCGTAAGATCCGTAG
TTTTGTTCGCCGCGAAGGCCGCTTAACAAAAGGCCAAGAAAACGCGATGAATGAATGTTGGCCAACAATGGGTATCGACT
ACAACCCAGAGCTTCTTAACTGGAAAGAAGTATTTGGCAATGATAACCCAGTTGTACTAGAGATTGGCTTCGGTATGGGT
GCATCACTGGTTGAAATGGCAAAGAACGCACCTGAGAAAAACTTCTTAGGTATTGAAGTTCACAGCCCAGGTGTTGGTGC
GTGTTTAGGTACAGCTCGCGATGCTGGCGTAACTAACCTGCGTGTAATGTGTCACGATGCAGTAGAAGTATTTGAACACA
TGATCCCAGACAGCAGCCTGCATACACTGCAACTGTTCTTCCCTGACCCATGGCATAAAGCTCGTCACCACAAACGTCGT
ATCGTTAAGGCTGAGTTTGCAGAGATGGTTCGCGGTAAGCTGCAACTTGATACTGGTATTTTCCACATGGCAACAGACTG
GGAAAACTACGCAGAACATATGATTGAAGTGATGAACGTAGCTCCAGGTTTCGAGAATATCGCTGAAGATGGTGATTACA
TTCCTCGTCCAGACGAGCGTCCACTGACTAAGTTTGAAGCTCGTGGCCACCGTTTAGGTCATGGTGTTTGGGACATTAAG
TTCAAGCGTACTAAGTAA

Upstream 100 bases:

>100_bases
TTAACGTTGGATGTAAAACAGACAAATTGTGTGGGAATTTATCCACGGAAAGACTTGCACCGAAGGCAATTCTTTGGATA
ATCCCCGCTTTGATTAATCA

Downstream 100 bases:

>100_bases
TTCTGAATCAAATTAAGTGAAGGTGAATCCGACTTAGGATTAACCCCTACACTTTATGGGATTGATAAAAGCCAACATTA
TTGTAGTGTTGGCTTTTTTG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MCRQKSMSEVTTNEYTEDGKLVRKIRSFVRREGRLTKGQENAMNECWPTMGIDYNPELLNWKEVFGNDNPVVLEIGFGMG
ASLVEMAKNAPEKNFLGIEVHSPGVGACLGTARDAGVTNLRVMCHDAVEVFEHMIPDSSLHTLQLFFPDPWHKARHHKRR
IVKAEFAEMVRGKLQLDTGIFHMATDWENYAEHMIEVMNVAPGFENIAEDGDYIPRPDERPLTKFEARGHRLGHGVWDIK
FKRTK

Sequences:

>Translated_245_residues
MCRQKSMSEVTTNEYTEDGKLVRKIRSFVRREGRLTKGQENAMNECWPTMGIDYNPELLNWKEVFGNDNPVVLEIGFGMG
ASLVEMAKNAPEKNFLGIEVHSPGVGACLGTARDAGVTNLRVMCHDAVEVFEHMIPDSSLHTLQLFFPDPWHKARHHKRR
IVKAEFAEMVRGKLQLDTGIFHMATDWENYAEHMIEVMNVAPGFENIAEDGDYIPRPDERPLTKFEARGHRLGHGVWDIK
FKRTK
>Mature_245_residues
MCRQKSMSEVTTNEYTEDGKLVRKIRSFVRREGRLTKGQENAMNECWPTMGIDYNPELLNWKEVFGNDNPVVLEIGFGMG
ASLVEMAKNAPEKNFLGIEVHSPGVGACLGTARDAGVTNLRVMCHDAVEVFEHMIPDSSLHTLQLFFPDPWHKARHHKRR
IVKAEFAEMVRGKLQLDTGIFHMATDWENYAEHMIEVMNVAPGFENIAEDGDYIPRPDERPLTKFEARGHRLGHGVWDIK
FKRTK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=238, Percent_Identity=64.2857142857143, Blast_Score=340, Evalue=6e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 28009; Mature: 28009

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRQKSMSEVTTNEYTEDGKLVRKIRSFVRREGRLTKGQENAMNECWPTMGIDYNPELLN
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
WKEVFGNDNPVVLEIGFGMGASLVEMAKNAPEKNFLGIEVHSPGVGACLGTARDAGVTNL
HHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHCCCCCCCCCHH
RVMCHDAVEVFEHMIPDSSLHTLQLFFPDPWHKARHHKRRIVKAEFAEMVRGKLQLDTGI
HHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCE
FHMATDWENYAEHMIEVMNVAPGFENIAEDGDYIPRPDERPLTKFEARGHRLGHGVWDIK
EEEECCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEE
FKRTK
EECCC
>Mature Secondary Structure
MCRQKSMSEVTTNEYTEDGKLVRKIRSFVRREGRLTKGQENAMNECWPTMGIDYNPELLN
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
WKEVFGNDNPVVLEIGFGMGASLVEMAKNAPEKNFLGIEVHSPGVGACLGTARDAGVTNL
HHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHCCCCCCCCCHH
RVMCHDAVEVFEHMIPDSSLHTLQLFFPDPWHKARHHKRRIVKAEFAEMVRGKLQLDTGI
HHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCE
FHMATDWENYAEHMIEVMNVAPGFENIAEDGDYIPRPDERPLTKFEARGHRLGHGVWDIK
EEEECCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEE
FKRTK
EECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA