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The map label for this gene is metK [H]

Identifier: 218710602

GI number: 218710602

Start: 2842937

End: 2844088

Strand: Direct

Name: metK [H]

Synonym: VS_2655

Alternate gene names: 218710602

Gene position: 2842937-2844088 (Clockwise)

Preceding gene: 218710600

Following gene: 218710603

Centisome position: 86.17

GC content: 46.53

Gene sequence:

>1152_bases
ATGGCTAAGCACCTATTCACTTCTGAATCTGTTTCAGAAGGCCATCCAGATAAAATTGCAGACCAAATCTCAGATGCTGT
TCTTGATGCCATCATTGAACAAGATCCAAAAGCACGTGTTGCTTGTGAGACTTACGTAAAAACCGGCATGGTTATGGTTG
GCGGTGAAGTAACCACGTCTGCATGGGTTGATATCGAAGAAATCACTCGTGAAACAGTTCGTGAAATTGGTTACGTTCAT
TCTGATATGGGCTTTGACGCTGACTCTTGTGCAGTACTAAACACAATTGGTAAGCAGTCTCCAGACATCAACCAAGGTGT
TGATAAAGCCGATCCTAAAGATCAAGGCGCAGGCGACCAAGGTATCATGTTTGGTTACGCGACTAACGAAACACCAATCC
TAATGCCAGCTCCAATTACTTACTCTCACCTTCTTGTTAAGAAGCAAGCTGAAGTACGTAAGAGCGGCAAGCTTGACTTC
CTTCGCCCAGATGCGAAATCTCAAGTAACGTTCCAATACGACCAAGGTAAGATCGTTGGTATCGACGCTGTTGTTCTTTC
AACTCAACACTGTGATTCAGTAACAACACCTGACCTACGTGAAGCGGTAATGGAAGAGATCATCAAGCCAGTACTTCCTG
CTGAGTGGATCAATAAAGACACTAACTTCTTCATCAACCCAACAGGCCGTTTCGTAATCGGTGGCCCAATGGGTGATTGT
GGTCTAACAGGTCGTAAGATCATCGTTGATACCTACGGCGGCGCTGCTCGTCACGGTGGCGGTGCATTCTCTGGTAAAGA
TCCATCAAAAGTTGACCGTTCTGCAGCTTACGCAGCTCGTTACGTTGCGAAAAACATCGTTGCTGCTGGCATGGCTGACC
GTTGTGAGATTCAACTGTCTTACGCTATCGGTGTTGCAGATCCAACATCTATCATGGTTGAAACGTTTGGTACTGAGAAA
GTAGCGCACGAAATCATCATTGAAGCAGTTCGTCAAAACTTCGACCTACGTCCATACGGTCTTCAAGAGATGCTGAACCT
TCTTCAACCTATCTACAAGCAGACTGCTGCATACGGCCACTTCGGTCGCGAAGAGTTCCCTTGGGAAGCAACTGACAAAG
CCGCTATCCTTGCGGACTTCGCAGGCCTGTAA

Upstream 100 bases:

>100_bases
ATATACTGTATTGAATGGCGGCGCTTCCTCGATTGCGACGGCATAAATTATTTGCATTTAACTATTTGCAACTAAAAACT
AAACTAAAAGTGGAGCTCTC

Downstream 100 bases:

>100_bases
GCATTACGCAACACGCTTAGCTGAAACGTAATTTTAAAAGCCTCTGTTCTCAGAGGCTTTTTTCTTTCTATTCTTCTATT
CTTCTATTCTTCTATTCTTC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MAKHLFTSESVSEGHPDKIADQISDAVLDAIIEQDPKARVACETYVKTGMVMVGGEVTTSAWVDIEEITRETVREIGYVH
SDMGFDADSCAVLNTIGKQSPDINQGVDKADPKDQGAGDQGIMFGYATNETPILMPAPITYSHLLVKKQAEVRKSGKLDF
LRPDAKSQVTFQYDQGKIVGIDAVVLSTQHCDSVTTPDLREAVMEEIIKPVLPAEWINKDTNFFINPTGRFVIGGPMGDC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQLSYAIGVADPTSIMVETFGTEK
VAHEIIIEAVRQNFDLRPYGLQEMLNLLQPIYKQTAAYGHFGREEFPWEATDKAAILADFAGL

Sequences:

>Translated_383_residues
MAKHLFTSESVSEGHPDKIADQISDAVLDAIIEQDPKARVACETYVKTGMVMVGGEVTTSAWVDIEEITRETVREIGYVH
SDMGFDADSCAVLNTIGKQSPDINQGVDKADPKDQGAGDQGIMFGYATNETPILMPAPITYSHLLVKKQAEVRKSGKLDF
LRPDAKSQVTFQYDQGKIVGIDAVVLSTQHCDSVTTPDLREAVMEEIIKPVLPAEWINKDTNFFINPTGRFVIGGPMGDC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQLSYAIGVADPTSIMVETFGTEK
VAHEIIIEAVRQNFDLRPYGLQEMLNLLQPIYKQTAAYGHFGREEFPWEATDKAAILADFAGL
>Mature_382_residues
AKHLFTSESVSEGHPDKIADQISDAVLDAIIEQDPKARVACETYVKTGMVMVGGEVTTSAWVDIEEITRETVREIGYVHS
DMGFDADSCAVLNTIGKQSPDINQGVDKADPKDQGAGDQGIMFGYATNETPILMPAPITYSHLLVKKQAEVRKSGKLDFL
RPDAKSQVTFQYDQGKIVGIDAVVLSTQHCDSVTTPDLREAVMEEIIKPVLPAEWINKDTNFFINPTGRFVIGGPMGDCG
LTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQLSYAIGVADPTSIMVETFGTEKV
AHEIIIEAVRQNFDLRPYGLQEMLNLLQPIYKQTAAYGHFGREEFPWEATDKAAILADFAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=374, Percent_Identity=55.6149732620321, Blast_Score=404, Evalue=1e-113,
Organism=Homo sapiens, GI4557737, Length=374, Percent_Identity=55.3475935828877, Blast_Score=402, Evalue=1e-112,
Organism=Escherichia coli, GI1789311, Length=383, Percent_Identity=84.0731070496084, Blast_Score=675, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17538498, Length=384, Percent_Identity=55.9895833333333, Blast_Score=406, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17538494, Length=377, Percent_Identity=56.2334217506631, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25145633, Length=384, Percent_Identity=55.46875, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17551082, Length=373, Percent_Identity=54.4235924932976, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI32565905, Length=377, Percent_Identity=50.9283819628647, Blast_Score=340, Evalue=8e-94,
Organism=Caenorhabditis elegans, GI32565911, Length=337, Percent_Identity=52.8189910979229, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI25145635, Length=337, Percent_Identity=52.2255192878338, Blast_Score=326, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI32565903, Length=299, Percent_Identity=53.8461538461538, Blast_Score=297, Evalue=8e-81,
Organism=Caenorhabditis elegans, GI32565909, Length=87, Percent_Identity=59.7701149425287, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI32565907, Length=46, Percent_Identity=71.7391304347826, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323209, Length=370, Percent_Identity=57.8378378378378, Blast_Score=412, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6320710, Length=374, Percent_Identity=56.4171122994652, Blast_Score=408, Evalue=1e-115,
Organism=Drosophila melanogaster, GI45552159, Length=373, Percent_Identity=54.9597855227882, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580529, Length=373, Percent_Identity=54.9597855227882, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580537, Length=373, Percent_Identity=54.9597855227882, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580533, Length=373, Percent_Identity=54.9597855227882, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580535, Length=373, Percent_Identity=54.9597855227882, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580531, Length=373, Percent_Identity=54.9597855227882, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580527, Length=373, Percent_Identity=54.9597855227882, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580525, Length=373, Percent_Identity=54.9597855227882, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580541, Length=372, Percent_Identity=52.6881720430108, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580539, Length=120, Percent_Identity=55.8333333333333, Blast_Score=131, Evalue=8e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 41600; Mature: 41468

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKHLFTSESVSEGHPDKIADQISDAVLDAIIEQDPKARVACETYVKTGMVMVGGEVTTS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCCCCH
AWVDIEEITRETVREIGYVHSDMGFDADSCAVLNTIGKQSPDINQGVDKADPKDQGAGDQ
HCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCC
GIMFGYATNETPILMPAPITYSHLLVKKQAEVRKSGKLDFLRPDAKSQVTFQYDQGKIVG
CEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEECCCEEEE
IDAVVLSTQHCDSVTTPDLREAVMEEIIKPVLPAEWINKDTNFFINPTGRFVIGGPMGDC
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQLS
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGVADPTSIMVETFGTEKVAHEIIIEAVRQNFDLRPYGLQEMLNLLQPIYKQTAAYGH
EEEECCCCHHHEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC
FGREEFPWEATDKAAILADFAGL
CCCCCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
AKHLFTSESVSEGHPDKIADQISDAVLDAIIEQDPKARVACETYVKTGMVMVGGEVTTS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCCCCH
AWVDIEEITRETVREIGYVHSDMGFDADSCAVLNTIGKQSPDINQGVDKADPKDQGAGDQ
HCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCC
GIMFGYATNETPILMPAPITYSHLLVKKQAEVRKSGKLDFLRPDAKSQVTFQYDQGKIVG
CEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEECCCEEEE
IDAVVLSTQHCDSVTTPDLREAVMEEIIKPVLPAEWINKDTNFFINPTGRFVIGGPMGDC
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQLS
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGVADPTSIMVETFGTEKVAHEIIIEAVRQNFDLRPYGLQEMLNLLQPIYKQTAAYGH
EEEECCCCHHHEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC
FGREEFPWEATDKAAILADFAGL
CCCCCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA