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The map label for this gene is gshA [H]

Identifier: 218710525

GI number: 218710525

Start: 2753189

End: 2754769

Strand: Reverse

Name: gshA [H]

Synonym: VS_2564

Alternate gene names: 218710525

Gene position: 2754769-2753189 (Counterclockwise)

Preceding gene: 218710526

Following gene: 218710524

Centisome position: 83.5

GC content: 44.47

Gene sequence:

>1581_bases
GTGAACAGAATTTTGACTGATTTTGCTGCGCGACTAAAGCAAGTTGCAACCAACCCTAAGACCTTCTCTCAATTTGGTCG
TGGTGTTGAAAGGGAAACATTGCGCTACACGGAAGATGGGCATCTTGCTACTGGGCCGCACCCTAAGGCTTTAGGATCTG
CGTTGATGAATGAATGGGTAACGACCGATTTCTCTGAGTCGCTACTGGAGTTTATCACGCCTGTTTCTAACGACGTTCCG
ACGCTTTTGAATCAGTTGTCTGATATCCATCACTTCACTCAAACCAAGTTAGACGGCGAAAAGCTGTGGCCACTCTCTAT
GCCTTGTTATGTGGGGAGTGAAGATTACATTCAGCTTGCGCAATACGGTACTTCTAACAACGGTAAGATGAAGACGCTAT
ATCGTGAAGGCCTAAAGCGTCGCTACGGTAGTCTGATGCAGATTATTTCGGGTGTTCACTTCAACTTCTCTTTCCCAGAA
AGCTTTTGGGACAGTCTGTTTGGAGAGCAAACCGAAGAAGCGCGTTGCGAAGCTAAATCAGATGCGTACTTCGGTTTGAT
TCGTAATTACTACCGCTTTGGTTGGTTAATCCCATACTTCTTCGGTGCTTCACCTGCGCTATGTTCTTCTTTTATCAAAG
GAAGAGAAACCAACCTACCTTTTGAAAAGGTTGGAGAAACACTGTATCTACCCAAAGCAACGGCATTGCGCTTAAGTGAC
CTTGGTTACACCAACAGTGCGCAAAGTGTACTGAAGATTGGCTTCAACAGCCTAGACCAATACCTTGAAGGTTTGAATCA
AGCGATTCGCACACCATCAGAAGAATTCGCTGAGATTGGCACTAAGGTTGATGGGGAATACCGTCAACTAAATAGCAACG
TACTGCAAATTGAGAATGAGCTTTATGCTCCAATCCGTCCTAAGCGAGTGGCGAAAAGTGGTGAGAAACCATCTGAAGCA
CTGGCTCGTGCTGGCGTTGAGTACATCGAGGTTCGCTCTTTAGACGTAAACCCATTTAGTTCAATTGGCATCAATGAACA
GCAAGTTCGCTTCTTAGATTTATTCCTAACGTGGAGCGTGTTATCAGACTCTGCTGAGATGGATAACTGCGAGCTTGAAT
GTTGGCGCGATAACTGGAATAAGGTGATCTTAGAAGGTCGTCAGGTTGGTTTAGAGCTCAAGATTGGTTGTGATGGAGAA
AGACTGTCTCTGCAAGACTGGGCTAAAAATGTATTCAAAGATCTTCGAGTTATCGCTGAGTTGATGGATGCTGAGCAAGG
TGGTCGTGCATACCAAGAAACGTGCGATACGCTTGAAGCCTGGATTGATAACCCAGAGTTGACCATTTCTGGTCAGTTAT
TAGAAGAGACCAAGAAATTGGGTGGTTTGGGTAAAGTGGGTTGTGCGTTAGGAAAAACGTATGCTCAGCAACACAAGGCA
CACCAATATAAAGTTTATTCTGCTGAGTTAATGGAAGCGGAAGTTCAACGCTCTGTCATTGCTCAACAGCAAAGTGAAGA
AGCGAGCACTCAAGATTTTGATAGTTTCTTAACGGACTATTTTTCATATTTAAAAGCATAG

Upstream 100 bases:

>100_bases
AATCATTCGTTAGAGTACACATTAAAGGGGGAGGGAAGAGATTCTACTTTTCTCCTCCAACCTAATTTATGATAGTTCTA
ATAAGAACAATATAAGATAA

Downstream 100 bases:

>100_bases
CGAGACTTTGGTGGAAAGGAAGCAAGCCTTATTAGGTCAGTCTACTCATGTGGGTAGAAAATGGTTACCTGTCGTGACTG
TTGGTATTGTCTCTAGCCTA

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MNRILTDFAARLKQVATNPKTFSQFGRGVERETLRYTEDGHLATGPHPKALGSALMNEWVTTDFSESLLEFITPVSNDVP
TLLNQLSDIHHFTQTKLDGEKLWPLSMPCYVGSEDYIQLAQYGTSNNGKMKTLYREGLKRRYGSLMQIISGVHFNFSFPE
SFWDSLFGEQTEEARCEAKSDAYFGLIRNYYRFGWLIPYFFGASPALCSSFIKGRETNLPFEKVGETLYLPKATALRLSD
LGYTNSAQSVLKIGFNSLDQYLEGLNQAIRTPSEEFAEIGTKVDGEYRQLNSNVLQIENELYAPIRPKRVAKSGEKPSEA
LARAGVEYIEVRSLDVNPFSSIGINEQQVRFLDLFLTWSVLSDSAEMDNCELECWRDNWNKVILEGRQVGLELKIGCDGE
RLSLQDWAKNVFKDLRVIAELMDAEQGGRAYQETCDTLEAWIDNPELTISGQLLEETKKLGGLGKVGCALGKTYAQQHKA
HQYKVYSAELMEAEVQRSVIAQQQSEEASTQDFDSFLTDYFSYLKA

Sequences:

>Translated_526_residues
MNRILTDFAARLKQVATNPKTFSQFGRGVERETLRYTEDGHLATGPHPKALGSALMNEWVTTDFSESLLEFITPVSNDVP
TLLNQLSDIHHFTQTKLDGEKLWPLSMPCYVGSEDYIQLAQYGTSNNGKMKTLYREGLKRRYGSLMQIISGVHFNFSFPE
SFWDSLFGEQTEEARCEAKSDAYFGLIRNYYRFGWLIPYFFGASPALCSSFIKGRETNLPFEKVGETLYLPKATALRLSD
LGYTNSAQSVLKIGFNSLDQYLEGLNQAIRTPSEEFAEIGTKVDGEYRQLNSNVLQIENELYAPIRPKRVAKSGEKPSEA
LARAGVEYIEVRSLDVNPFSSIGINEQQVRFLDLFLTWSVLSDSAEMDNCELECWRDNWNKVILEGRQVGLELKIGCDGE
RLSLQDWAKNVFKDLRVIAELMDAEQGGRAYQETCDTLEAWIDNPELTISGQLLEETKKLGGLGKVGCALGKTYAQQHKA
HQYKVYSAELMEAEVQRSVIAQQQSEEASTQDFDSFLTDYFSYLKA
>Mature_526_residues
MNRILTDFAARLKQVATNPKTFSQFGRGVERETLRYTEDGHLATGPHPKALGSALMNEWVTTDFSESLLEFITPVSNDVP
TLLNQLSDIHHFTQTKLDGEKLWPLSMPCYVGSEDYIQLAQYGTSNNGKMKTLYREGLKRRYGSLMQIISGVHFNFSFPE
SFWDSLFGEQTEEARCEAKSDAYFGLIRNYYRFGWLIPYFFGASPALCSSFIKGRETNLPFEKVGETLYLPKATALRLSD
LGYTNSAQSVLKIGFNSLDQYLEGLNQAIRTPSEEFAEIGTKVDGEYRQLNSNVLQIENELYAPIRPKRVAKSGEKPSEA
LARAGVEYIEVRSLDVNPFSSIGINEQQVRFLDLFLTWSVLSDSAEMDNCELECWRDNWNKVILEGRQVGLELKIGCDGE
RLSLQDWAKNVFKDLRVIAELMDAEQGGRAYQETCDTLEAWIDNPELTISGQLLEETKKLGGLGKVGCALGKTYAQQHKA
HQYKVYSAELMEAEVQRSVIAQQQSEEASTQDFDSFLTDYFSYLKA

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=518, Percent_Identity=53.6679536679537, Blast_Score=570, Evalue=1e-164,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 59516; Mature: 59516

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRILTDFAARLKQVATNPKTFSQFGRGVERETLRYTEDGHLATGPHPKALGSALMNEWV
CCCHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
TTDFSESLLEFITPVSNDVPTLLNQLSDIHHFTQTKLDGEKLWPLSMPCYVGSEDYIQLA
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCHHHHHHH
QYGTSNNGKMKTLYREGLKRRYGSLMQIISGVHFNFSFPESFWDSLFGEQTEEARCEAKS
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCHHHHHHHCC
DAYFGLIRNYYRFGWLIPYFFGASPALCSSFIKGRETNLPFEKVGETLYLPKATALRLSD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHCCCEEECCCHHHHHHHH
LGYTNSAQSVLKIGFNSLDQYLEGLNQAIRTPSEEFAEIGTKVDGEYRQLNSNVLQIENE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHCCHHHEEHHH
LYAPIRPKRVAKSGEKPSEALARAGVEYIEVRSLDVNPFSSIGINEQQVRFLDLFLTWSV
HCCCCCHHHHHHCCCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
LSDSAEMDNCELECWRDNWNKVILEGRQVGLELKIGCDGERLSLQDWAKNVFKDLRVIAE
HHCCCCCCCCCHHHHHCCCCCEEEECCEECEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
LMDAEQGGRAYQETCDTLEAWIDNPELTISGQLLEETKKLGGLGKVGCALGKTYAQQHKA
HHHHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
HQYKVYSAELMEAEVQRSVIAQQQSEEASTQDFDSFLTDYFSYLKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNRILTDFAARLKQVATNPKTFSQFGRGVERETLRYTEDGHLATGPHPKALGSALMNEWV
CCCHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
TTDFSESLLEFITPVSNDVPTLLNQLSDIHHFTQTKLDGEKLWPLSMPCYVGSEDYIQLA
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCHHHHHHH
QYGTSNNGKMKTLYREGLKRRYGSLMQIISGVHFNFSFPESFWDSLFGEQTEEARCEAKS
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCHHHHHHHCC
DAYFGLIRNYYRFGWLIPYFFGASPALCSSFIKGRETNLPFEKVGETLYLPKATALRLSD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHCCCEEECCCHHHHHHHH
LGYTNSAQSVLKIGFNSLDQYLEGLNQAIRTPSEEFAEIGTKVDGEYRQLNSNVLQIENE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHCCHHHEEHHH
LYAPIRPKRVAKSGEKPSEALARAGVEYIEVRSLDVNPFSSIGINEQQVRFLDLFLTWSV
HCCCCCHHHHHHCCCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
LSDSAEMDNCELECWRDNWNKVILEGRQVGLELKIGCDGERLSLQDWAKNVFKDLRVIAE
HHCCCCCCCCCHHHHHCCCCCEEEECCEECEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
LMDAEQGGRAYQETCDTLEAWIDNPELTISGQLLEETKKLGGLGKVGCALGKTYAQQHKA
HHHHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
HQYKVYSAELMEAEVQRSVIAQQQSEEASTQDFDSFLTDYFSYLKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA