Click here to switch to the map view.

The map label for this gene is mrcB [H]

Identifier: 218710485

GI number: 218710485

Start: 2712500

End: 2714899

Strand: Reverse

Name: mrcB [H]

Synonym: VS_2524

Alternate gene names: 218710485

Gene position: 2714899-2712500 (Counterclockwise)

Preceding gene: 218710487

Following gene: 218710484

Centisome position: 82.29

GC content: 47.04

Gene sequence:

>2400_bases
ATGATGACCAAAATGTTAACGCCAAAAAAGGCACCACCTAAAAAAGCGCCAGCAAAGAAGTCACCAGCGACCAAAGGCAA
GCCTAGAAAGCCAAGATCAGCAGCGAAAAAGACTAAACCTAAGGCTAAGCCGTCTGGCAAGAGAGGTTGGTTGAAGATTC
TGTGGGGCATTTCTTGGAAAGCTGGCTTAGCACTTGCCGCATTGCTGTTGTTCGTCGGTATTTATCTGGATTCAGTGGTT
AAGCAGCGTTTTGAAGGTCAGCTTTTTGATCTGCCAACCGTTGTTTATGCTCGTGTGTTGGATCTGTCTCCGGGGACTGC
GGTGAGTTTGGTACAGGTTAAGAATGAACTGGATGTACTTAATTACCGTAAAGTGAATTCTCCTCGTCACCCAGGTGAGT
ACTCTTCTTCTTCAACCAAGATTGAAATGATTCGTCGTCCGTTCGAGTTTGTCGATGGACCGGAAGCGGATCGTCATGTA
ATGCTGCATTTCAACGGTAACGAGTTAACTCGTATACAGTCGCTAGAGAAGAAAGGCGACATGGGCTATCTACGTGTTGA
GCCGAAAATGTTGGGTATGCTTGAAAAAAGTAACGATGAGCAACGCCTGTTCTTAAAACGTAATCAGTTCCCAGAAGTGA
TGGTTGATGCCTTATTAGCAACTGAAGACCGAAGCTTCTATCAACACGATGGGGTATCGCCATTAGCAATCGCTCGTGCA
ATGGTCGTTAACGTCAAAGCTGGGCGAACGGTACAGGGGGGTAGTACGCTGACACAACAGCTGGCAAAAAACCTGTTCTT
GTCCAGTGAACGTACATTGTGGCGTAAGGTTCGTGAAGCCTACATTGCGCTTATCTTGGACCATCGTTACAGCAAAGACC
GTATTCTAGAGGCTTACTTAAACGAGGTTTACCTTGGGCAAAATGGTGGGCAAGCGATTCATGGTTTCGGCTTGGCATCT
CGTTTGTATTTCGGTCAACCTATTCAAGAGCTCCGCATCGATCAACTAGCATTGCTGGTGGGTATGGTGAAAGGCCCTTC
GTATTACAATCCGGTTCGTTACCCTGAGCGTGCTAAGACTCGACGCGATCTGGTTCTTCGCTTATTGATGCAGCAAGACA
TATTAACTCCGCGACAATACGAAGAAGCGGCGAGTCGTGATTTGGATATTCAAGACAATCCACGTATTGCTAGTCGTCAG
CCAGCTTACTTCCAGCAAGTAAACATTGAGCTTAAGAAGTATGTCGGCGATCGATTTGAAGCGAAAAAAGGGATTCGAGT
CTTTACCTCTCTGGATCCCGTTTCCCAAGACAAGTTAGAAAAGTCGATTGCTCGTAAAGTGCCTGATTTATCGAAAACGG
CAGGTAACAAACTGGAAGCCGCAGCTATTGCCGTGGATCGCAATACTGGTGAAATCCGAGCGATGGTTGGTGGTAAACGT
ACCGGTTACGATGGTTTTAACCGTGCTTTGAATGCGAGTCGCCCTATTGGTTCTTTGGTTAAGCCTGCCATCTACTTAAC
CGCATTGGAACAACCTCAGAAGTATACGTTGGCGACAACATTGAAGGACACCCCACTGAGCTTGAAAGGCAGTAAGGGCA
GCGTATGGAGCCCAAGAAACTTCGATCGTAAGTTCCGTGGCGATGTGCCCTTGTATGTGGCGCTTTCTAAGTCTTATAAC
GTACCTACCGTTCGTTTAGGCATGCAGTTAGGCATTGATAGCGTTTCGGACACCATTGGTAAGCTGGGTGTCGATAAAAA
TGAGATTCGCCCAGTACCGTCGATGTTTTTGGGTTCGTTCTCACTGACTCCGTTCCAAGTTGCTCAGATGTACCAAACCA
TTACCAACTCTGGTCGCATTGCGCCTTTGTCTGCACTTCGTTCTGTTGTTGATAATGATGGCGAAGTTCTGTATCAATCG
ATTCCTCGTGTATCGCAAAGCGTTGACCAGCAGGCAGCTTGGTTAACAACCTATGCGATGAAGCGGGGTGTATCGGAAGG
TACAGGTCGTTTCTTACAAGGTCAGTTTGCATGGGCTGGGTTAGCTGGTAAAACGGGCACAAGTAATGACAGTCGAGACA
GTTGGTTCGTGGGTGTTGATGGTCGTGAAGTCACGACGATTTGGTTAGGCCGAGATGATAATAAACCGACTAAACTAACC
GGTTCTAGCGGTGCGTTGCGTGTCTATGCCGATTATCTGAAGCAAAGAACGCCTGAACAACTATTACTGCCTTGGCCAAC
AGGAATTGCGACCGCTAGCTTTACTCGAACTTCTGAAGGGGCGTTAGAGTTTGACTGTGGTGGTACAGTCAAATTGCCGG
TCTGGGATGAAAGTGGCAGCATTAAAAAGGGCTGTGAAAGTCAACCAAAACAGTGGCTAAAGAAGCTTTTTCAGTGGTAA

Upstream 100 bases:

>100_bases
AAGTACAAAAAGAGATGAAAGGCCGTTACCCCAAACATCCTTGGCCGGATGACCCTGCTAATCATGTAGCAACCACTAAA
ACTAAACGACAGTTGAACCG

Downstream 100 bases:

>100_bases
AGTCATAACAGAAAAGAATGACAAGGATAGTTTAGATAATGATTTCTCGTTGGTTAGTGAGTAGCTTGGGTATTGTCGGT
ACTGTGATCAGTTTTCAACT

Product: penicillin-binding protein 1B

Products: NA

Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 799; Mature: 799

Protein sequence:

>799_residues
MMTKMLTPKKAPPKKAPAKKSPATKGKPRKPRSAAKKTKPKAKPSGKRGWLKILWGISWKAGLALAALLLFVGIYLDSVV
KQRFEGQLFDLPTVVYARVLDLSPGTAVSLVQVKNELDVLNYRKVNSPRHPGEYSSSSTKIEMIRRPFEFVDGPEADRHV
MLHFNGNELTRIQSLEKKGDMGYLRVEPKMLGMLEKSNDEQRLFLKRNQFPEVMVDALLATEDRSFYQHDGVSPLAIARA
MVVNVKAGRTVQGGSTLTQQLAKNLFLSSERTLWRKVREAYIALILDHRYSKDRILEAYLNEVYLGQNGGQAIHGFGLAS
RLYFGQPIQELRIDQLALLVGMVKGPSYYNPVRYPERAKTRRDLVLRLLMQQDILTPRQYEEAASRDLDIQDNPRIASRQ
PAYFQQVNIELKKYVGDRFEAKKGIRVFTSLDPVSQDKLEKSIARKVPDLSKTAGNKLEAAAIAVDRNTGEIRAMVGGKR
TGYDGFNRALNASRPIGSLVKPAIYLTALEQPQKYTLATTLKDTPLSLKGSKGSVWSPRNFDRKFRGDVPLYVALSKSYN
VPTVRLGMQLGIDSVSDTIGKLGVDKNEIRPVPSMFLGSFSLTPFQVAQMYQTITNSGRIAPLSALRSVVDNDGEVLYQS
IPRVSQSVDQQAAWLTTYAMKRGVSEGTGRFLQGQFAWAGLAGKTGTSNDSRDSWFVGVDGREVTTIWLGRDDNKPTKLT
GSSGALRVYADYLKQRTPEQLLLPWPTGIATASFTRTSEGALEFDCGGTVKLPVWDESGSIKKGCESQPKQWLKKLFQW

Sequences:

>Translated_799_residues
MMTKMLTPKKAPPKKAPAKKSPATKGKPRKPRSAAKKTKPKAKPSGKRGWLKILWGISWKAGLALAALLLFVGIYLDSVV
KQRFEGQLFDLPTVVYARVLDLSPGTAVSLVQVKNELDVLNYRKVNSPRHPGEYSSSSTKIEMIRRPFEFVDGPEADRHV
MLHFNGNELTRIQSLEKKGDMGYLRVEPKMLGMLEKSNDEQRLFLKRNQFPEVMVDALLATEDRSFYQHDGVSPLAIARA
MVVNVKAGRTVQGGSTLTQQLAKNLFLSSERTLWRKVREAYIALILDHRYSKDRILEAYLNEVYLGQNGGQAIHGFGLAS
RLYFGQPIQELRIDQLALLVGMVKGPSYYNPVRYPERAKTRRDLVLRLLMQQDILTPRQYEEAASRDLDIQDNPRIASRQ
PAYFQQVNIELKKYVGDRFEAKKGIRVFTSLDPVSQDKLEKSIARKVPDLSKTAGNKLEAAAIAVDRNTGEIRAMVGGKR
TGYDGFNRALNASRPIGSLVKPAIYLTALEQPQKYTLATTLKDTPLSLKGSKGSVWSPRNFDRKFRGDVPLYVALSKSYN
VPTVRLGMQLGIDSVSDTIGKLGVDKNEIRPVPSMFLGSFSLTPFQVAQMYQTITNSGRIAPLSALRSVVDNDGEVLYQS
IPRVSQSVDQQAAWLTTYAMKRGVSEGTGRFLQGQFAWAGLAGKTGTSNDSRDSWFVGVDGREVTTIWLGRDDNKPTKLT
GSSGALRVYADYLKQRTPEQLLLPWPTGIATASFTRTSEGALEFDCGGTVKLPVWDESGSIKKGCESQPKQWLKKLFQW
>Mature_799_residues
MMTKMLTPKKAPPKKAPAKKSPATKGKPRKPRSAAKKTKPKAKPSGKRGWLKILWGISWKAGLALAALLLFVGIYLDSVV
KQRFEGQLFDLPTVVYARVLDLSPGTAVSLVQVKNELDVLNYRKVNSPRHPGEYSSSSTKIEMIRRPFEFVDGPEADRHV
MLHFNGNELTRIQSLEKKGDMGYLRVEPKMLGMLEKSNDEQRLFLKRNQFPEVMVDALLATEDRSFYQHDGVSPLAIARA
MVVNVKAGRTVQGGSTLTQQLAKNLFLSSERTLWRKVREAYIALILDHRYSKDRILEAYLNEVYLGQNGGQAIHGFGLAS
RLYFGQPIQELRIDQLALLVGMVKGPSYYNPVRYPERAKTRRDLVLRLLMQQDILTPRQYEEAASRDLDIQDNPRIASRQ
PAYFQQVNIELKKYVGDRFEAKKGIRVFTSLDPVSQDKLEKSIARKVPDLSKTAGNKLEAAAIAVDRNTGEIRAMVGGKR
TGYDGFNRALNASRPIGSLVKPAIYLTALEQPQKYTLATTLKDTPLSLKGSKGSVWSPRNFDRKFRGDVPLYVALSKSYN
VPTVRLGMQLGIDSVSDTIGKLGVDKNEIRPVPSMFLGSFSLTPFQVAQMYQTITNSGRIAPLSALRSVVDNDGEVLYQS
IPRVSQSVDQQAAWLTTYAMKRGVSEGTGRFLQGQFAWAGLAGKTGTSNDSRDSWFVGVDGREVTTIWLGRDDNKPTKLT
GSSGALRVYADYLKQRTPEQLLLPWPTGIATASFTRTSEGALEFDCGGTVKLPVWDESGSIKKGCESQPKQWLKKLFQW

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=730, Percent_Identity=48.9041095890411, Blast_Score=677, Evalue=0.0,
Organism=Escherichia coli, GI87082258, Length=264, Percent_Identity=33.7121212121212, Blast_Score=172, Evalue=6e-44,
Organism=Escherichia coli, GI1788867, Length=513, Percent_Identity=29.0448343079922, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1789601, Length=168, Percent_Identity=30.952380952381, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011813
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: =2.4.1.129 [H]

Molecular weight: Translated: 89149; Mature: 89149

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTKMLTPKKAPPKKAPAKKSPATKGKPRKPRSAAKKTKPKAKPSGKRGWLKILWGISWK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCEEEEEECCCHH
AGLALAALLLFVGIYLDSVVKQRFEGQLFDLPTVVYARVLDLSPGTAVSLVQVKNELDVL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEEHHHHHHHH
NYRKVNSPRHPGEYSSSSTKIEMIRRPFEFVDGPEADRHVMLHFNGNELTRIQSLEKKGD
HHEECCCCCCCCCCCCCCHHHHHHHCCHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCC
MGYLRVEPKMLGMLEKSNDEQRLFLKRNQFPEVMVDALLATEDRSFYQHDGVSPLAIARA
CCEEEECHHHHHHHHCCCCCCEEHHHCCCCHHHHHHHHHHHCCCCHHHHCCCCHHHHHHH
MVVNVKAGRTVQGGSTLTQQLAKNLFLSSERTLWRKVREAYIALILDHRYSKDRILEAYL
HHEEECCCCEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NEVYLGQNGGQAIHGFGLASRLYFGQPIQELRIDQLALLVGMVKGPSYYNPVRYPERAKT
HHEEECCCCCCEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
RRDLVLRLLMQQDILTPRQYEEAASRDLDIQDNPRIASRQPAYFQQVNIELKKYVGDRFE
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHH
AKKGIRVFTSLDPVSQDKLEKSIARKVPDLSKTAGNKLEAAAIAVDRNTGEIRAMVGGKR
HCCCCEEEECCCCCCHHHHHHHHHHHCCCHHHHCCCCEEEEEEEEECCCCCEEEEECCCC
TGYDGFNRALNASRPIGSLVKPAIYLTALEQPQKYTLATTLKDTPLSLKGSKGSVWSPRN
CCCCHHHHHHCCCCCHHHHHHHHHEEEECCCCCCEEEEEEECCCCCEEECCCCCCCCCCC
FDRKFRGDVPLYVALSKSYNVPTVRLGMQLGIDSVSDTIGKLGVDKNEIRPVPSMFLGSF
CCHHHCCCCCEEEEEECCCCCCEEEEHHHHCCCHHHHHHHHCCCCHHHCCCCHHHHHCCC
SLTPFQVAQMYQTITNSGRIAPLSALRSVVDNDGEVLYQSIPRVSQSVDQQAAWLTTYAM
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KRGVSEGTGRFLQGQFAWAGLAGKTGTSNDSRDSWFVGVDGREVTTIWLGRDDNKPTKLT
HCCCCCCCCCEEECCEEEEECCCCCCCCCCCCCCEEEECCCCEEEEEEEECCCCCCCEEE
GSSGALRVYADYLKQRTPEQLLLPWPTGIATASFTRTSEGALEFDCGGTVKLPVWDESGS
CCCCCHHHHHHHHHHCCCCCEEEECCCCCCEEEECCCCCCCEEECCCCEEEEEEECCCCC
IKKGCESQPKQWLKKLFQW
HHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MMTKMLTPKKAPPKKAPAKKSPATKGKPRKPRSAAKKTKPKAKPSGKRGWLKILWGISWK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCEEEEEECCCHH
AGLALAALLLFVGIYLDSVVKQRFEGQLFDLPTVVYARVLDLSPGTAVSLVQVKNELDVL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEEHHHHHHHH
NYRKVNSPRHPGEYSSSSTKIEMIRRPFEFVDGPEADRHVMLHFNGNELTRIQSLEKKGD
HHEECCCCCCCCCCCCCCHHHHHHHCCHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCC
MGYLRVEPKMLGMLEKSNDEQRLFLKRNQFPEVMVDALLATEDRSFYQHDGVSPLAIARA
CCEEEECHHHHHHHHCCCCCCEEHHHCCCCHHHHHHHHHHHCCCCHHHHCCCCHHHHHHH
MVVNVKAGRTVQGGSTLTQQLAKNLFLSSERTLWRKVREAYIALILDHRYSKDRILEAYL
HHEEECCCCEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NEVYLGQNGGQAIHGFGLASRLYFGQPIQELRIDQLALLVGMVKGPSYYNPVRYPERAKT
HHEEECCCCCCEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
RRDLVLRLLMQQDILTPRQYEEAASRDLDIQDNPRIASRQPAYFQQVNIELKKYVGDRFE
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHH
AKKGIRVFTSLDPVSQDKLEKSIARKVPDLSKTAGNKLEAAAIAVDRNTGEIRAMVGGKR
HCCCCEEEECCCCCCHHHHHHHHHHHCCCHHHHCCCCEEEEEEEEECCCCCEEEEECCCC
TGYDGFNRALNASRPIGSLVKPAIYLTALEQPQKYTLATTLKDTPLSLKGSKGSVWSPRN
CCCCHHHHHHCCCCCHHHHHHHHHEEEECCCCCCEEEEEEECCCCCEEECCCCCCCCCCC
FDRKFRGDVPLYVALSKSYNVPTVRLGMQLGIDSVSDTIGKLGVDKNEIRPVPSMFLGSF
CCHHHCCCCCEEEEEECCCCCCEEEEHHHHCCCHHHHHHHHCCCCHHHCCCCHHHHHCCC
SLTPFQVAQMYQTITNSGRIAPLSALRSVVDNDGEVLYQSIPRVSQSVDQQAAWLTTYAM
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KRGVSEGTGRFLQGQFAWAGLAGKTGTSNDSRDSWFVGVDGREVTTIWLGRDDNKPTKLT
HCCCCCCCCCEEECCEEEEECCCCCCCCCCCCCCEEEECCCCEEEEEEEECCCCCCCEEE
GSSGALRVYADYLKQRTPEQLLLPWPTGIATASFTRTSEGALEFDCGGTVKLPVWDESGS
CCCCCHHHHHHHHHHCCCCCEEEECCCCCCEEEECCCCCCCEEECCCCEEEEEEECCCCC
IKKGCESQPKQWLKKLFQW
HHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]