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The map label for this gene is amtB [C]

Identifier: 218710480

GI number: 218710480

Start: 2704777

End: 2706006

Strand: Reverse

Name: amtB [C]

Synonym: VS_2519

Alternate gene names: 218710480

Gene position: 2706006-2704777 (Counterclockwise)

Preceding gene: 218710481

Following gene: 218710479

Centisome position: 82.02

GC content: 46.1

Gene sequence:

>1230_bases
ATGGAACTTACAACAACAGTAACGGAACTACGTTACGCACTAGACACTTTTTTCTTCCTCATTTCAGGTGCGTTGGTAAT
GTGGATGGCTGCAGGCTTTGCGATGTTAGAAGCTGGCCTCGTTCGTTCAAAGAACACCACAGAAATTTTAACTAAGAACA
TTTGTTTGTACGCAATTGCTTGTACGACTTTCTTAGTCGTTGGTTACAACATTATGTATGTAGATAACGGCGAAGGTGGC
TGGTTGCCGTCATTTGGTACTCTGATTGGTACTCAAGGTGAAGGTGCAGACCACTCATTAGAATCAGACTTCTTCTTCCA
AGTCGTTTTCGTTGCAACAGCAATGTCTGTGGTATCAGGTGCGGTTGCTGAGCGAATGAAACTTTGGTCATTCCTTATCT
TCTCTGTCGTGCTAACAGCATTCATCTACCCAATGGAAGGTTACTGGACTTGGGGCGGTGGTTTTCTATCAGAAGCTGGT
TTCAGTGACTTTGCTGGTTCAGGTATCGTACACATGGCCGGTGCTTCAGCAGCGTTAGCGGGTGTGCTTCTACTGGGGGC
TCGTAAAGGTAAATATGGTAAGAACGGTGAAATCTACCCGATTCCAGGTTCAAACATGCCACTGGCAACGCTAGGTACAT
TTATCCTTTGGTTTGGTTGGTTCGGTTTCAACGGCGGTTCTCAACTAATGGTTTCGGATTTTGAGAACGCAACAGCAGTA
GGTCAAATCTTCCTTAACACCAACGCAGCAGCTGCAGCAGGTGCGATTGCAGCACTACTAGTATGTAAAACAACTTGGGG
TAAAGCCGACCTAACAATGATTCTGAACGGTGCGTTAGCTGGCCTAGTTGCAATCACTGCAGATCCTCTATCTCCATCCC
CCCTATTTTCGGTAGCGATTGGTTCTGTATCTGGTGCCTTGGTTGTATTCAGTATCATTGCTCTAGATAAAGCTAAGATT
GACGATCCAGTGGGTGCTATCTCGGTACACGGTGTATGTGGTTTCTTCGGTTTAATGGCTGTTCCACTAAGCAATGCTGA
TGCAACATTTGGTGCTCAATTGTTGGGTGCCGCAGTCATCTTTGCATGGGTATTCGGAGCGAGCTTAGCGGTATGGGCAG
TGCTTAAAGCGACAATCGGTATCCGAGTAACTGAAGATGAAGAGCTGGAAGGTATGGACATGCACGATTGTGGTGTTGGC
GCTTACCCAGAGTTTGTGTCTGTAAAATAG

Upstream 100 bases:

>100_bases
CAAAATTTTTGTGTATGACCTGAGCCAAGCCGTACGAATTCGTACCGGTGAAATGGATGCTGAAGCACTTTAAAGAATTA
CAAGGACTGGAGACTTTAAT

Downstream 100 bases:

>100_bases
AACGTTGTTAACTAATCTATAATTTACAAACATTTACATATTACCCACTTGAAACCTGCTCTCATGAGCAGGTTTTTTTG
TGTTCTATCATTGAAATTAA

Product: ammonium transporter family protein

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MELTTTVTELRYALDTFFFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNICLYAIACTTFLVVGYNIMYVDNGEGG
WLPSFGTLIGTQGEGADHSLESDFFFQVVFVATAMSVVSGAVAERMKLWSFLIFSVVLTAFIYPMEGYWTWGGGFLSEAG
FSDFAGSGIVHMAGASAALAGVLLLGARKGKYGKNGEIYPIPGSNMPLATLGTFILWFGWFGFNGGSQLMVSDFENATAV
GQIFLNTNAAAAAGAIAALLVCKTTWGKADLTMILNGALAGLVAITADPLSPSPLFSVAIGSVSGALVVFSIIALDKAKI
DDPVGAISVHGVCGFFGLMAVPLSNADATFGAQLLGAAVIFAWVFGASLAVWAVLKATIGIRVTEDEELEGMDMHDCGVG
AYPEFVSVK

Sequences:

>Translated_409_residues
MELTTTVTELRYALDTFFFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNICLYAIACTTFLVVGYNIMYVDNGEGG
WLPSFGTLIGTQGEGADHSLESDFFFQVVFVATAMSVVSGAVAERMKLWSFLIFSVVLTAFIYPMEGYWTWGGGFLSEAG
FSDFAGSGIVHMAGASAALAGVLLLGARKGKYGKNGEIYPIPGSNMPLATLGTFILWFGWFGFNGGSQLMVSDFENATAV
GQIFLNTNAAAAAGAIAALLVCKTTWGKADLTMILNGALAGLVAITADPLSPSPLFSVAIGSVSGALVVFSIIALDKAKI
DDPVGAISVHGVCGFFGLMAVPLSNADATFGAQLLGAAVIFAWVFGASLAVWAVLKATIGIRVTEDEELEGMDMHDCGVG
AYPEFVSVK
>Mature_409_residues
MELTTTVTELRYALDTFFFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNICLYAIACTTFLVVGYNIMYVDNGEGG
WLPSFGTLIGTQGEGADHSLESDFFFQVVFVATAMSVVSGAVAERMKLWSFLIFSVVLTAFIYPMEGYWTWGGGFLSEAG
FSDFAGSGIVHMAGASAALAGVLLLGARKGKYGKNGEIYPIPGSNMPLATLGTFILWFGWFGFNGGSQLMVSDFENATAV
GQIFLNTNAAAAAGAIAALLVCKTTWGKADLTMILNGALAGLVAITADPLSPSPLFSVAIGSVSGALVVFSIIALDKAKI
DDPVGAISVHGVCGFFGLMAVPLSNADATFGAQLLGAAVIFAWVFGASLAVWAVLKATIGIRVTEDEELEGMDMHDCGVG
AYPEFVSVK

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=411, Percent_Identity=30.9002433090024, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17550180, Length=438, Percent_Identity=33.3333333333333, Blast_Score=201, Evalue=7e-52,
Organism=Caenorhabditis elegans, GI17550182, Length=426, Percent_Identity=31.6901408450704, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI32564042, Length=436, Percent_Identity=27.5229357798165, Blast_Score=144, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI133931100, Length=342, Percent_Identity=28.9473684210526, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6325396, Length=422, Percent_Identity=29.3838862559242, Blast_Score=122, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6321559, Length=442, Percent_Identity=28.7330316742081, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324187, Length=417, Percent_Identity=28.0575539568345, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161078314, Length=404, Percent_Identity=30.9405940594059, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 42863; Mature: 42863

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELTTTVTELRYALDTFFFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNICLYAIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
CTTFLVVGYNIMYVDNGEGGWLPSFGTLIGTQGEGADHSLESDFFFQVVFVATAMSVVSG
HHHHHHHCCEEEEEECCCCCCCCCHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AVAERMKLWSFLIFSVVLTAFIYPMEGYWTWGGGFLSEAGFSDFAGSGIVHMAGASAALA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHCCCCCCCCCCCEEEECCHHHHHH
GVLLLGARKGKYGKNGEIYPIPGSNMPLATLGTFILWFGWFGFNGGSQLMVSDFENATAV
HHHHHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEE
GQIFLNTNAAAAAGAIAALLVCKTTWGKADLTMILNGALAGLVAITADPLSPSPLFSVAI
EEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCHHHHHH
GSVSGALVVFSIIALDKAKIDDPVGAISVHGVCGFFGLMAVPLSNADATFGAQLLGAAVI
HCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
FAWVFGASLAVWAVLKATIGIRVTEDEELEGMDMHDCGVGAYPEFVSVK
HHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCCCCCCCCCCCEECC
>Mature Secondary Structure
MELTTTVTELRYALDTFFFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNICLYAIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
CTTFLVVGYNIMYVDNGEGGWLPSFGTLIGTQGEGADHSLESDFFFQVVFVATAMSVVSG
HHHHHHHCCEEEEEECCCCCCCCCHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AVAERMKLWSFLIFSVVLTAFIYPMEGYWTWGGGFLSEAGFSDFAGSGIVHMAGASAALA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHCCCCCCCCCCCEEEECCHHHHHH
GVLLLGARKGKYGKNGEIYPIPGSNMPLATLGTFILWFGWFGFNGGSQLMVSDFENATAV
HHHHHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEE
GQIFLNTNAAAAAGAIAALLVCKTTWGKADLTMILNGALAGLVAITADPLSPSPLFSVAI
EEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCHHHHHH
GSVSGALVVFSIIALDKAKIDDPVGAISVHGVCGFFGLMAVPLSNADATFGAQLLGAAVI
HCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
FAWVFGASLAVWAVLKATIGIRVTEDEELEGMDMHDCGVGAYPEFVSVK
HHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]