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The map label for this gene is rrmJ [H]

Identifier: 218710452

GI number: 218710452

Start: 2663684

End: 2664313

Strand: Reverse

Name: rrmJ [H]

Synonym: VS_2491

Alternate gene names: 218710452

Gene position: 2664313-2663684 (Counterclockwise)

Preceding gene: 218710454

Following gene: 218710451

Centisome position: 80.75

GC content: 43.49

Gene sequence:

>630_bases
ATGAGCAAACAGAAACACTCGGCCAGTTCGGGCCGTTGGTTGAAGGAACACTTCGACGACAAGTACGCAAATGAAGCCAG
AAAAAAAGGCTATCGTTCGCGTGCTTATTTCAAAATGGAAGAGATTCAAACGAAAGATAAATTGCTGACTCCAGGGATGA
CCATTGTGGATTTGGGTGCAGCACCTGGCGGTTGGTCTCAATATGCTGCTAAGATTATCGGAGACAACGGTCAAATCATT
GCGTGTGATTTGTTACCAATGGATCCGATTGCTGGAGTGAGCTTTTTACAAGGCGATTTCCGCGAAGAAGCTGTGCTAGA
TGCGTTGCTGGAACGTATACAACCAAACATGGTTGATGTCGTGATGTCTGATATGGCACCTAATATAGCAGGCAACAATT
CTGTGGATCAGCCAAGAGCTATGTATTTAGTTGAATTAGCTTTGGATATGTGTCGACAAGTTCTAGCTACCAATGGTAGT
TTTGTTGTAAAAGTATTCCAAGGCGAAGGGTTTGACCAATATGTTAAGGACGTCCGTGAGATGTTTAAAACAGTCAAAGT
TAGAAAACCCGACTCTTCTCGAGCTCGCTCTCGTGAAGTGTTTATCGTAGCAACTGGTTACAAAGGTTAA

Upstream 100 bases:

>100_bases
TATTTAGTATTAGGGTTGAAAAAACCTATTTTAACGCCATCTAAAGCTGAAGACTATAATTTATTCAGCAATTAGTACTT
TCCTCCAATTTAGGTATCCA

Downstream 100 bases:

>100_bases
CGATTCTCTTCCAAGAGTGAGAGTTAACAACATGGCTACAGGTTACAAACTGTAGTACCCTACCTTTAATTACAATTAGT
TATCGAGAGGCTTACACCTT

Product: 23S rRNA methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MSKQKHSASSGRWLKEHFDDKYANEARKKGYRSRAYFKMEEIQTKDKLLTPGMTIVDLGAAPGGWSQYAAKIIGDNGQII
ACDLLPMDPIAGVSFLQGDFREEAVLDALLERIQPNMVDVVMSDMAPNIAGNNSVDQPRAMYLVELALDMCRQVLATNGS
FVVKVFQGEGFDQYVKDVREMFKTVKVRKPDSSRARSREVFIVATGYKG

Sequences:

>Translated_209_residues
MSKQKHSASSGRWLKEHFDDKYANEARKKGYRSRAYFKMEEIQTKDKLLTPGMTIVDLGAAPGGWSQYAAKIIGDNGQII
ACDLLPMDPIAGVSFLQGDFREEAVLDALLERIQPNMVDVVMSDMAPNIAGNNSVDQPRAMYLVELALDMCRQVLATNGS
FVVKVFQGEGFDQYVKDVREMFKTVKVRKPDSSRARSREVFIVATGYKG
>Mature_208_residues
SKQKHSASSGRWLKEHFDDKYANEARKKGYRSRAYFKMEEIQTKDKLLTPGMTIVDLGAAPGGWSQYAAKIIGDNGQIIA
CDLLPMDPIAGVSFLQGDFREEAVLDALLERIQPNMVDVVMSDMAPNIAGNNSVDQPRAMYLVELALDMCRQVLATNGSF
VVKVFQGEGFDQYVKDVREMFKTVKVRKPDSSRARSREVFIVATGYKG

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=206, Percent_Identity=32.5242718446602, Blast_Score=130, Evalue=7e-31,
Organism=Homo sapiens, GI194097365, Length=188, Percent_Identity=35.1063829787234, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI29029591, Length=190, Percent_Identity=33.6842105263158, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI29029589, Length=190, Percent_Identity=33.6842105263158, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI7110661, Length=190, Percent_Identity=33.6842105263158, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1789569, Length=208, Percent_Identity=71.1538461538462, Blast_Score=315, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI17553474, Length=220, Percent_Identity=33.1818181818182, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71987550, Length=189, Percent_Identity=35.4497354497354, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71987561, Length=189, Percent_Identity=35.4497354497354, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17553860, Length=189, Percent_Identity=35.4497354497354, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17554650, Length=191, Percent_Identity=32.9842931937173, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6319796, Length=210, Percent_Identity=32.8571428571429, Blast_Score=114, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6319535, Length=198, Percent_Identity=33.8383838383838, Blast_Score=114, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321302, Length=283, Percent_Identity=27.5618374558304, Blast_Score=90, Evalue=2e-19,
Organism=Drosophila melanogaster, GI18859957, Length=188, Percent_Identity=36.1702127659575, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21356387, Length=212, Percent_Identity=34.4339622641509, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=32.3232323232323, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648639, Length=193, Percent_Identity=33.6787564766839, Blast_Score=99, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 23352; Mature: 23221

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQKHSASSGRWLKEHFDDKYANEARKKGYRSRAYFKMEEIQTKDKLLTPGMTIVDLGA
CCCCCCCCCCCCHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCC
APGGWSQYAAKIIGDNGQIIACDLLPMDPIAGVSFLQGDFREEAVLDALLERIQPNMVDV
CCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VMSDMAPNIAGNNSVDQPRAMYLVELALDMCRQVLATNGSFVVKVFQGEGFDQYVKDVRE
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHH
MFKTVKVRKPDSSRARSREVFIVATGYKG
HHHHHCCCCCCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure 
SKQKHSASSGRWLKEHFDDKYANEARKKGYRSRAYFKMEEIQTKDKLLTPGMTIVDLGA
CCCCCCCCCCCHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCC
APGGWSQYAAKIIGDNGQIIACDLLPMDPIAGVSFLQGDFREEAVLDALLERIQPNMVDV
CCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VMSDMAPNIAGNNSVDQPRAMYLVELALDMCRQVLATNGSFVVKVFQGEGFDQYVKDVRE
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHH
MFKTVKVRKPDSSRARSREVFIVATGYKG
HHHHHCCCCCCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA