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The map label for this gene is uraA [H]

Identifier: 218710282

GI number: 218710282

Start: 2482794

End: 2484032

Strand: Direct

Name: uraA [H]

Synonym: VS_2316

Alternate gene names: 218710282

Gene position: 2482794-2484032 (Clockwise)

Preceding gene: 218710280

Following gene: 218710284

Centisome position: 75.25

GC content: 46.49

Gene sequence:

>1239_bases
ATGAAAAATGCTCTGCAAGGTGCGCAAATGCTATTTGTCGCTTTTGGTGCACTTGTACTGGTGCCCCTGCTGACAGGCCT
TGATCCCAACGTAGCGCTCTTTGGGGCAGGTATCGGTACCCTTTTATTCCAACTTATTACACGCCGTTCAGTACCTATTT
TCCTTGCGTCTTCTTTTGCATTCATCGCACCTATCATGTTTGGCATTCAAACTTGGGGTGTAGGTGCAACCATGGGCGGC
CTTATGGCAGCAGGTGTTGTGTATGTATTAATGGGTGCCTTAATTAAGGTACGTGGTGTAGCCTTTATCCACAAACTGCT
TCCACCAGTCGTAGTTGGCCCTGTAATCATGGTGATCGGCTTAGGTCTTGCGCCTGTTGCGGTAAACATGGCGCTAGGTA
AAACAGGCGATGGTGCGGTTCAGCTTATAGATGCAGACGCAGCATTATGGATTTCATCTATTTCATTGTTAGTAACGATT
GTCATCAGTGTTTTCTCGAAAGGCTTTCTTAAACTGCTGCCGATCTTCGGCGGTATTGTGGCTGGTTATGTAACGAGCTT
AGTATACGGTGTCGTTGATTTTACCCCTGTCGCTCAGGCCGCTTGGTTAGCTCTACCTAACTTCACAGCGCCAGAGTTCA
ACATCAACGCTATCTTCTTTATGGTATTTGTAGCAATTGCACCTGCTGTTGAACACGTAGGCGACATGCTTGCTATTTCG
AATGTAACCGGTAAAGACTACCTTAAGAAGCCAGGTTTACACCGCACAATAACTGGTGACGGCGTGGCAACGATTGCTGC
TTCTATGTTGGGTGCTCCACCAAATACAACCTACAGTGAAGTAACGGGGGCTGTAATGCTGACCAAAGCATTCAATCCAG
TGATCATGACTTGGGCAGCAGTAACAGCGATTGTTCTAGCATTAGTAGGTAAGCTAGGCGCACTACTTCAAACGATTCCA
GTTCCTGTTATGGGCGGCATCATGATTCTTCTGTTTGGTTCTATCGCAACCGTAGGCTTGAATACTTTAATTCAGAACAA
AGTCGACCTACACAAATCACGTAACCTTGTGATTGTCGGCATTACTTTAGTCTTCGGTATTGGCGGCATGGCATTTGGCA
TCGGCGACTTTAGCCTGCAAGGCGTAAGCTTATGCGGTATCGTTGCTATTCTACTGAACCTAGTCCTTCCTGAAGAGCTA
GGCGACAACACCGTAGTAGATAAAGCTCAAATCGACTAA

Upstream 100 bases:

>100_bases
AAATTTGATCTATCTGACATAGCAACCGATTGCCAAGTGATAGAATCCCGCGAATTTTATTTTTCGCTTTTCATATAACG
ACCATTTTTAAGGACATAAC

Downstream 100 bases:

>100_bases
CTGCAAGTCAACAAACTGTAGAGGCTTGTAATCCTCAATTAAGAGCATCACAAGCTAGCAAGATCAAAAAAGGGAGAGCA
TCGAATGCTCTCCCTTTTTA

Product: uracil permease

Products: Proton [Cytoplasm]; uracil [Cytoplasm] [C]

Alternate protein names: Uracil transporter [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MKNALQGAQMLFVAFGALVLVPLLTGLDPNVALFGAGIGTLLFQLITRRSVPIFLASSFAFIAPIMFGIQTWGVGATMGG
LMAAGVVYVLMGALIKVRGVAFIHKLLPPVVVGPVIMVIGLGLAPVAVNMALGKTGDGAVQLIDADAALWISSISLLVTI
VISVFSKGFLKLLPIFGGIVAGYVTSLVYGVVDFTPVAQAAWLALPNFTAPEFNINAIFFMVFVAIAPAVEHVGDMLAIS
NVTGKDYLKKPGLHRTITGDGVATIAASMLGAPPNTTYSEVTGAVMLTKAFNPVIMTWAAVTAIVLALVGKLGALLQTIP
VPVMGGIMILLFGSIATVGLNTLIQNKVDLHKSRNLVIVGITLVFGIGGMAFGIGDFSLQGVSLCGIVAILLNLVLPEEL
GDNTVVDKAQID

Sequences:

>Translated_412_residues
MKNALQGAQMLFVAFGALVLVPLLTGLDPNVALFGAGIGTLLFQLITRRSVPIFLASSFAFIAPIMFGIQTWGVGATMGG
LMAAGVVYVLMGALIKVRGVAFIHKLLPPVVVGPVIMVIGLGLAPVAVNMALGKTGDGAVQLIDADAALWISSISLLVTI
VISVFSKGFLKLLPIFGGIVAGYVTSLVYGVVDFTPVAQAAWLALPNFTAPEFNINAIFFMVFVAIAPAVEHVGDMLAIS
NVTGKDYLKKPGLHRTITGDGVATIAASMLGAPPNTTYSEVTGAVMLTKAFNPVIMTWAAVTAIVLALVGKLGALLQTIP
VPVMGGIMILLFGSIATVGLNTLIQNKVDLHKSRNLVIVGITLVFGIGGMAFGIGDFSLQGVSLCGIVAILLNLVLPEEL
GDNTVVDKAQID
>Mature_412_residues
MKNALQGAQMLFVAFGALVLVPLLTGLDPNVALFGAGIGTLLFQLITRRSVPIFLASSFAFIAPIMFGIQTWGVGATMGG
LMAAGVVYVLMGALIKVRGVAFIHKLLPPVVVGPVIMVIGLGLAPVAVNMALGKTGDGAVQLIDADAALWISSISLLVTI
VISVFSKGFLKLLPIFGGIVAGYVTSLVYGVVDFTPVAQAAWLALPNFTAPEFNINAIFFMVFVAIAPAVEHVGDMLAIS
NVTGKDYLKKPGLHRTITGDGVATIAASMLGAPPNTTYSEVTGAVMLTKAFNPVIMTWAAVTAIVLALVGKLGALLQTIP
VPVMGGIMILLFGSIATVGLNTLIQNKVDLHKSRNLVIVGITLVFGIGGMAFGIGDFSLQGVSLCGIVAILLNLVLPEEL
GDNTVVDKAQID

Specific function: Transport of uracil in the cell [H]

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily [H]

Homologues:

Organism=Homo sapiens, GI44680145, Length=433, Percent_Identity=24.4803695150115, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI44680143, Length=437, Percent_Identity=24.2562929061785, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI44680148, Length=429, Percent_Identity=23.0769230769231, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI40316845, Length=429, Percent_Identity=23.0769230769231, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788843, Length=411, Percent_Identity=38.6861313868613, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI87081818, Length=417, Percent_Identity=37.410071942446, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI87082181, Length=381, Percent_Identity=28.0839895013123, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87082178, Length=313, Percent_Identity=27.1565495207668, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790087, Length=373, Percent_Identity=23.8605898123324, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17558856, Length=177, Percent_Identity=29.9435028248588, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17542262, Length=173, Percent_Identity=27.1676300578035, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI21356175, Length=173, Percent_Identity=31.2138728323699, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006042
- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 42649; Mature: 42649

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; uracil [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uracil [Periplasm] = Proton [Cytoplasm] + uracil [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]