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The map label for this gene is cysK [H]

Identifier: 218710256

GI number: 218710256

Start: 2460337

End: 2461305

Strand: Direct

Name: cysK [H]

Synonym: VS_2289

Alternate gene names: 218710256

Gene position: 2460337-2461305 (Clockwise)

Preceding gene: 218710255

Following gene: 218710257

Centisome position: 74.57

GC content: 46.03

Gene sequence:

>969_bases
ATGAGCAAGATCTACGAAGACAACACCCTAACGATTGGTAACACACCTTTAGTCCGCCTAAACAAGGTAAGCAAAGGTAA
CGTCCTAGCTAAGATCGAAGCTCGTAACCCAAGCTTCAGCGTTAAGTGTCGTATCGGTTCAAACATGATCTGGGAAGCAG
AAAAAGCAGGTACGCTTAAGCCAGGTATCGAGCTTGTTGAGCCTACCAGTGGTAATACAGGTGTTGCTCTTGCATTCGTA
GCTGCAGCGCGCGGTTACAAGCTAACACTAACAATGCCTGAATCAATGAGCCTAGAACGTCGTAAGCTGCTTAAAGCTCT
TGGCGCAAACCTAGTGCTAACTGAAGCACCAAAAGGTATGAACGGCGCGATTGCTAAAGCTGAAGAGATTGTTGCTTCAG
ACCCAGACAAATACCTACTACTGCAACAATTCAACAACCCAGCGAACCCACAAATTCACGAGCAGACAACTGGCCCTGAA
ATTTGGGAAGCAACAGATGGTGAGATCGATGTGTTTGTAGCCGGTGTAGGTACAGGTGGCACTATCACTGGAACAAGTCG
TTACATTAAAGGTGAAAAAGGCAAAGCCATCACTTCTGTAGCCGTTGAACCAGCAGAATCTCCAGTGATTGCGCAGGCAC
TTGCAGGTGAAGAAATCAAACCAGCACCACACAAAATCCAAGGTATTGGCGCAGGTTTCATTCCTGGCAACCTAGATTTA
GACATTATTGACCGTGTAGAGTCAGTAACTTCTGAAGAAGCAATTGAGATGGCTCAACGCCTAATGAAAGAAGAAGGTAT
CCTAGCGGGCATCTCATCTGGTGCAGCAGTAGTAGCAGCAAACAGAATTTCGGAACTACCTGAATTTGCAGGGAAAACTA
TCGTTACTGTACTACCAAGCTCGGGCGAGCGTTACCTTAGTACGGCCCTATTTGCTGGTATCTTTACGGAAAAAGAGAAC
CAACAATAA

Upstream 100 bases:

>100_bases
GTTTAATCTAAATTCAAGCTAAACAAACATCGAAAGACACTAGACGGTAAATTGGTAGATATACTACGTTTAGTTCTGTC
ATTAAATGAAGGAATATCGC

Downstream 100 bases:

>100_bases
TATGTGGTCAAATCAATTTTTCGTCCAAAAAAGCCCCATTTAGGGGCTTTTTTGTTGATCCCTGCCTCACCTTTGGTAAT
ATCGGGTCTGTTTTATTTTT

Product: cysteine synthase A

Products: NA

Alternate protein names: CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; Sulfate starvation-induced protein 5; SSI5 [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSKIYEDNTLTIGNTPLVRLNKVSKGNVLAKIEARNPSFSVKCRIGSNMIWEAEKAGTLKPGIELVEPTSGNTGVALAFV
AAARGYKLTLTMPESMSLERRKLLKALGANLVLTEAPKGMNGAIAKAEEIVASDPDKYLLLQQFNNPANPQIHEQTTGPE
IWEATDGEIDVFVAGVGTGGTITGTSRYIKGEKGKAITSVAVEPAESPVIAQALAGEEIKPAPHKIQGIGAGFIPGNLDL
DIIDRVESVTSEEAIEMAQRLMKEEGILAGISSGAAVVAANRISELPEFAGKTIVTVLPSSGERYLSTALFAGIFTEKEN
QQ

Sequences:

>Translated_322_residues
MSKIYEDNTLTIGNTPLVRLNKVSKGNVLAKIEARNPSFSVKCRIGSNMIWEAEKAGTLKPGIELVEPTSGNTGVALAFV
AAARGYKLTLTMPESMSLERRKLLKALGANLVLTEAPKGMNGAIAKAEEIVASDPDKYLLLQQFNNPANPQIHEQTTGPE
IWEATDGEIDVFVAGVGTGGTITGTSRYIKGEKGKAITSVAVEPAESPVIAQALAGEEIKPAPHKIQGIGAGFIPGNLDL
DIIDRVESVTSEEAIEMAQRLMKEEGILAGISSGAAVVAANRISELPEFAGKTIVTVLPSSGERYLSTALFAGIFTEKEN
QQ
>Mature_321_residues
SKIYEDNTLTIGNTPLVRLNKVSKGNVLAKIEARNPSFSVKCRIGSNMIWEAEKAGTLKPGIELVEPTSGNTGVALAFVA
AARGYKLTLTMPESMSLERRKLLKALGANLVLTEAPKGMNGAIAKAEEIVASDPDKYLLLQQFNNPANPQIHEQTTGPEI
WEATDGEIDVFVAGVGTGGTITGTSRYIKGEKGKAITSVAVEPAESPVIAQALAGEEIKPAPHKIQGIGAGFIPGNLDLD
IIDRVESVTSEEAIEMAQRLMKEEGILAGISSGAAVVAANRISELPEFAGKTIVTVLPSSGERYLSTALFAGIFTEKENQ
Q

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=320, Percent_Identity=40, Blast_Score=205, Evalue=4e-53,
Organism=Homo sapiens, GI295821200, Length=320, Percent_Identity=40, Blast_Score=205, Evalue=4e-53,
Organism=Homo sapiens, GI4557415, Length=320, Percent_Identity=40, Blast_Score=205, Evalue=4e-53,
Organism=Escherichia coli, GI1788754, Length=323, Percent_Identity=77.7089783281734, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI2367138, Length=304, Percent_Identity=42.7631578947368, Blast_Score=220, Evalue=9e-59,
Organism=Caenorhabditis elegans, GI17535051, Length=315, Percent_Identity=50.4761904761905, Blast_Score=291, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI115535073, Length=317, Percent_Identity=54.2586750788643, Blast_Score=287, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI17562970, Length=318, Percent_Identity=48.1132075471698, Blast_Score=283, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI32566674, Length=307, Percent_Identity=49.185667752443, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI17561720, Length=316, Percent_Identity=48.7341772151899, Blast_Score=259, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI32566672, Length=226, Percent_Identity=46.0176991150442, Blast_Score=201, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI17534315, Length=321, Percent_Identity=41.1214953271028, Blast_Score=160, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI25147552, Length=325, Percent_Identity=36.3076923076923, Blast_Score=147, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17561716, Length=131, Percent_Identity=47.3282442748092, Blast_Score=104, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6321594, Length=316, Percent_Identity=36.7088607594937, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6321449, Length=323, Percent_Identity=34.984520123839, Blast_Score=134, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24643623, Length=321, Percent_Identity=35.5140186915888, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI20129101, Length=321, Percent_Identity=35.5140186915888, Blast_Score=157, Evalue=9e-39,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34085; Mature: 33954

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIYEDNTLTIGNTPLVRLNKVSKGNVLAKIEARNPSFSVKCRIGSNMIWEAEKAGTLK
CCCEECCCEEEECCCCEEEEEECCCCCEEEEEEECCCCEEEEEEECCCEEEECCCCCCCC
PGIELVEPTSGNTGVALAFVAAARGYKLTLTMPESMSLERRKLLKALGANLVLTEAPKGM
CCCEEECCCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCC
NGAIAKAEEIVASDPDKYLLLQQFNNPANPQIHEQTTGPEIWEATDGEIDVFVAGVGTGG
CCHHHHHHHHHHCCCCCEEEEEECCCCCCCCEECCCCCCCCEECCCCCEEEEEEEECCCC
TITGTSRYIKGEKGKAITSVAVEPAESPVIAQALAGEEIKPAPHKIQGIGAGFIPGNLDL
EECCCCEEEECCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCH
DIIDRVESVTSEEAIEMAQRLMKEEGILAGISSGAAVVAANRISELPEFAGKTIVTVLPS
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEHHHHHHHHHHCCCEEEEEECC
SGERYLSTALFAGIFTEKENQQ
CCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SKIYEDNTLTIGNTPLVRLNKVSKGNVLAKIEARNPSFSVKCRIGSNMIWEAEKAGTLK
CCEECCCEEEECCCCEEEEEECCCCCEEEEEEECCCCEEEEEEECCCEEEECCCCCCCC
PGIELVEPTSGNTGVALAFVAAARGYKLTLTMPESMSLERRKLLKALGANLVLTEAPKGM
CCCEEECCCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCC
NGAIAKAEEIVASDPDKYLLLQQFNNPANPQIHEQTTGPEIWEATDGEIDVFVAGVGTGG
CCHHHHHHHHHHCCCCCEEEEEECCCCCCCCEECCCCCCCCEECCCCCEEEEEEEECCCC
TITGTSRYIKGEKGKAITSVAVEPAESPVIAQALAGEEIKPAPHKIQGIGAGFIPGNLDL
EECCCCEEEECCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCH
DIIDRVESVTSEEAIEMAQRLMKEEGILAGISSGAAVVAANRISELPEFAGKTIVTVLPS
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEHHHHHHHHHHCCCEEEEEECC
SGERYLSTALFAGIFTEKENQQ
CCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]