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The map label for this gene is ligA

Identifier: 218710253

GI number: 218710253

Start: 2455871

End: 2457883

Strand: Reverse

Name: ligA

Synonym: VS_2286

Alternate gene names: 218710253

Gene position: 2457883-2455871 (Counterclockwise)

Preceding gene: 218710254

Following gene: 218710252

Centisome position: 74.5

GC content: 47.09

Gene sequence:

>2013_bases
ATGAAAGAATCGATTCAAGTTACCTTAGAGCAGTTAAGAGAAACTCTGCACTATCATGCCGTTCGTTATTACGTAGAAGA
TAGCCCTGAGATCCCTGATGTCGAGTACGATCGATTGATGCAACAACTGCTAAAGATCGAAGATGAGAACCCAGAACTTG
TGACGGTAGATTCGCCGAGTCAGCGTGTCGGCGGTCAGCCTCTAGACGGCTTCACTCAAGTGACACATGAGATCCCGATG
CTTTCTTTAGATAATGCTTTCTCTGATGACGATTTAGATGCGTTCAATAAGCGCATGTCTGATAGAGCGCCAACGGCGAA
CTTAGAGACTTTTTGTTGTGAGCCTAAACTTGATGGTTTAGCTGTGAGCCTACTCTATGTAAACGGCACCTTAGTACAGG
CAGCGACGCGTGGTGATGGTGCGACTGGCGAAAATATTACCGAAAACGTGCGTACCATCAGCTCGATTCCGCTTAAGTTA
CAAGGTGAAGGCTGGCCAGAACGTATTGAAGTCCGTGGCGAAGTGTTTATGCCAAAAGCGGGCTTCGACAAATTGAATGA
GATGGCATTGAAGAAGGGTGAGAAGGTCTTTGTGAATCCACGTAATGCCGCCGCAGGTAGCTTACGTCAGCTTGATTCTC
GTATTACAGCAAAACGCCCCTTGGCTTTCTACGCATACAGTGTCGGTGTTGTACAAGGCGCTGAGCTTTCAAATAGCCAT
TATCAACGTTTTCTGCAGCTGAAAGCTTGGGGCTTACCTATGTGCCCAGAGACAAAGCAGCTAAGTTCTCTTGAGGATGT
AAAAGCCTATTACCAAGATATCATGACTCGTCGTGACACCTTGGCTTATGAAATTGATGGGGTGGTGATTAAGGTTGATG
ACATTGCCGCACAAGAAACTCTAGGTTTTGTTGCACGTGCACCTCGCTGGGCTATTGCTTATAAGTTCCCAGCTCAAGAA
GAAATTACTCTGCTCAATGATGTTGAGTTTCAGGTTGGTCGTACAGGCGCTATTACGCCGGTTGCTAAGCTTGAACCTAT
TTTTGTTGGCGGCGTGACGGTGAGTAATGCAACCCTGCACAACGCTGATGAGATCGCTCGTTTAGGTGTGAAGGTCGGAG
ATAGCGTTATTATCCGCCGCGCTGGTGACGTCATTCCACAAATTGTAGCTGTTGTACAAGATCGTCGTCCTGAGACGGCT
AAAGACATTGTGTTCCCGGACGCCTGCCCTGTATGTAATTCTGCAGTGGAGCGCGTAGAGGGCGAAGCTGTAGCGCGTTG
TACTGGCGGTTTAGTGTGTCAGGCGCAGCGTAAAGAGGCACTTAAGCACTTTGTGTCTAGAAAGGCGCTGGATGTCGATG
GCCTTGGCGTAAAGGTGATAGAACAGCTTGTGGATCGCGAAATGGTCGAAACGCCAGCGGACCTGTTTAAGTTGAGCGCG
GGCGTGATTACGGTTCTTGATCGTATGGGACCTAAATCGGCACAGAATGTAGTGAGTGCGCTTAACAAAGCGAAAGACAC
GACATTGGCACGCTTCCTTTATTCTTTAGGAATTCGAGAAGTCGGTGAAGCGACGGCTATGAACTTAGCTCAGCACTTTA
AAACGCTAGAGTTGGTTCAAGCTGCAACTCATGAGCAGTTGGTTGAAGTGTCAGATATTGGTGACATCGTAGCAAGTCAC
CTCACGAGCTTCTTCTCACAAGAGAAAAACAGAGCAGTAGTCGACCAACTTATCGAGCTTGGAGTTAACTGGCCTGCAAT
TGAAGCTGTTGCGGATGATCAAGAGCTACCGTTAGAAGGCAAAGTAGTTGTGTTGACTGGCTCACTTTCTAAATTAGGTC
GCAGCGAAGCGAAAGCTGCTTTGCAAGCTTTAGGTGCAAAAGTAACCGGTAGCGTGTCTAAGAAAACGGATATCTTATTT
GCTGGTGAAGCCGCTGGTTCTAAACTGACTAAAGCACAAGATTTAGGTATCGAAATAAGAACAGAAGAAGATCTAATAGC
CCTTATTTCGTAA

Upstream 100 bases:

>100_bases
ACGCCTAGCCGTATTGTTTAGCCTTGTAAATAATCTATATTTATAGAAAAGGGCTCCTAAGGGAGCCCTTTTTGATATTT
CAATCACGACAGAGAATGAT

Downstream 100 bases:

>100_bases
GTAAATACAGATAAAAAAAGCTCAAAGGCACTCCTTTCAAGGGAGTGCCTTTTTCGTTTCTGATATAAAAGTCATCACTT
TTTAATTGTTGAGAAATTTG

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 670; Mature: 670

Protein sequence:

>670_residues
MKESIQVTLEQLRETLHYHAVRYYVEDSPEIPDVEYDRLMQQLLKIEDENPELVTVDSPSQRVGGQPLDGFTQVTHEIPM
LSLDNAFSDDDLDAFNKRMSDRAPTANLETFCCEPKLDGLAVSLLYVNGTLVQAATRGDGATGENITENVRTISSIPLKL
QGEGWPERIEVRGEVFMPKAGFDKLNEMALKKGEKVFVNPRNAAAGSLRQLDSRITAKRPLAFYAYSVGVVQGAELSNSH
YQRFLQLKAWGLPMCPETKQLSSLEDVKAYYQDIMTRRDTLAYEIDGVVIKVDDIAAQETLGFVARAPRWAIAYKFPAQE
EITLLNDVEFQVGRTGAITPVAKLEPIFVGGVTVSNATLHNADEIARLGVKVGDSVIIRRAGDVIPQIVAVVQDRRPETA
KDIVFPDACPVCNSAVERVEGEAVARCTGGLVCQAQRKEALKHFVSRKALDVDGLGVKVIEQLVDREMVETPADLFKLSA
GVITVLDRMGPKSAQNVVSALNKAKDTTLARFLYSLGIREVGEATAMNLAQHFKTLELVQAATHEQLVEVSDIGDIVASH
LTSFFSQEKNRAVVDQLIELGVNWPAIEAVADDQELPLEGKVVVLTGSLSKLGRSEAKAALQALGAKVTGSVSKKTDILF
AGEAAGSKLTKAQDLGIEIRTEEDLIALIS

Sequences:

>Translated_670_residues
MKESIQVTLEQLRETLHYHAVRYYVEDSPEIPDVEYDRLMQQLLKIEDENPELVTVDSPSQRVGGQPLDGFTQVTHEIPM
LSLDNAFSDDDLDAFNKRMSDRAPTANLETFCCEPKLDGLAVSLLYVNGTLVQAATRGDGATGENITENVRTISSIPLKL
QGEGWPERIEVRGEVFMPKAGFDKLNEMALKKGEKVFVNPRNAAAGSLRQLDSRITAKRPLAFYAYSVGVVQGAELSNSH
YQRFLQLKAWGLPMCPETKQLSSLEDVKAYYQDIMTRRDTLAYEIDGVVIKVDDIAAQETLGFVARAPRWAIAYKFPAQE
EITLLNDVEFQVGRTGAITPVAKLEPIFVGGVTVSNATLHNADEIARLGVKVGDSVIIRRAGDVIPQIVAVVQDRRPETA
KDIVFPDACPVCNSAVERVEGEAVARCTGGLVCQAQRKEALKHFVSRKALDVDGLGVKVIEQLVDREMVETPADLFKLSA
GVITVLDRMGPKSAQNVVSALNKAKDTTLARFLYSLGIREVGEATAMNLAQHFKTLELVQAATHEQLVEVSDIGDIVASH
LTSFFSQEKNRAVVDQLIELGVNWPAIEAVADDQELPLEGKVVVLTGSLSKLGRSEAKAALQALGAKVTGSVSKKTDILF
AGEAAGSKLTKAQDLGIEIRTEEDLIALIS
>Mature_670_residues
MKESIQVTLEQLRETLHYHAVRYYVEDSPEIPDVEYDRLMQQLLKIEDENPELVTVDSPSQRVGGQPLDGFTQVTHEIPM
LSLDNAFSDDDLDAFNKRMSDRAPTANLETFCCEPKLDGLAVSLLYVNGTLVQAATRGDGATGENITENVRTISSIPLKL
QGEGWPERIEVRGEVFMPKAGFDKLNEMALKKGEKVFVNPRNAAAGSLRQLDSRITAKRPLAFYAYSVGVVQGAELSNSH
YQRFLQLKAWGLPMCPETKQLSSLEDVKAYYQDIMTRRDTLAYEIDGVVIKVDDIAAQETLGFVARAPRWAIAYKFPAQE
EITLLNDVEFQVGRTGAITPVAKLEPIFVGGVTVSNATLHNADEIARLGVKVGDSVIIRRAGDVIPQIVAVVQDRRPETA
KDIVFPDACPVCNSAVERVEGEAVARCTGGLVCQAQRKEALKHFVSRKALDVDGLGVKVIEQLVDREMVETPADLFKLSA
GVITVLDRMGPKSAQNVVSALNKAKDTTLARFLYSLGIREVGEATAMNLAQHFKTLELVQAATHEQLVEVSDIGDIVASH
LTSFFSQEKNRAVVDQLIELGVNWPAIEAVADDQELPLEGKVVVLTGSLSKLGRSEAKAALQALGAKVTGSVSKKTDILF
AGEAAGSKLTKAQDLGIEIRTEEDLIALIS

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=668, Percent_Identity=63.9221556886228, Blast_Score=884, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=501, Percent_Identity=22.1556886227545, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_VIBSL (B7VIK3)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002417874.1
- GeneID:   7161828
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_2286
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 73181; Mature: 73181

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 116-116 BINDING 114-114 BINDING 137-137 BINDING 174-174 BINDING 291-291 BINDING 315-315

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKESIQVTLEQLRETLHYHAVRYYVEDSPEIPDVEYDRLMQQLLKIEDENPELVTVDSPS
CCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCH
QRVGGQPLDGFTQVTHEIPMLSLDNAFSDDDLDAFNKRMSDRAPTANLETFCCEPKLDGL
HHCCCCCCCHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCE
AVSLLYVNGTLVQAATRGDGATGENITENVRTISSIPLKLQGEGWPERIEVRGEVFMPKA
EEEEEEECCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCEEEECCEEECCCC
GFDKLNEMALKKGEKVFVNPRNAAAGSLRQLDSRITAKRPLAFYAYSVGVVQGAELSNSH
CHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHEECCCCCHHH
YQRFLQLKAWGLPMCPETKQLSSLEDVKAYYQDIMTRRDTLAYEIDGVVIKVDDIAAQET
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEECEEEEEECCHHHHHH
LGFVARAPRWAIAYKFPAQEEITLLNDVEFQVGRTGAITPVAKLEPIFVGGVTVSNATLH
HHHHHCCCCEEEEEECCCCCCEEEEECCEEEECCCCCCCCHHHCCEEEECCEEECCCCCC
NADEIARLGVKVGDSVIIRRAGDVIPQIVAVVQDRRPETAKDIVFPDACPVCNSAVERVE
CHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHC
GEAVARCTGGLVCQAQRKEALKHFVSRKALDVDGLGVKVIEQLVDREMVETPADLFKLSA
CCHHHHHCCCEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
GVITVLDRMGPKSAQNVVSALNKAKDTTLARFLYSLGIREVGEATAMNLAQHFKTLELVQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
AATHEQLVEVSDIGDIVASHLTSFFSQEKNRAVVDQLIELGVNWPAIEAVADDQELPLEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHEECCCCCCCCCC
KVVVLTGSLSKLGRSEAKAALQALGAKVTGSVSKKTDILFAGEAAGSKLTKAQDLGIEIR
EEEEEECCHHHHCHHHHHHHHHHHCCHHCCCCCCCCEEEEECCCCCCCCCHHHHCCEEEE
TEEDLIALIS
CCCCEEEECC
>Mature Secondary Structure
MKESIQVTLEQLRETLHYHAVRYYVEDSPEIPDVEYDRLMQQLLKIEDENPELVTVDSPS
CCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCH
QRVGGQPLDGFTQVTHEIPMLSLDNAFSDDDLDAFNKRMSDRAPTANLETFCCEPKLDGL
HHCCCCCCCHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCE
AVSLLYVNGTLVQAATRGDGATGENITENVRTISSIPLKLQGEGWPERIEVRGEVFMPKA
EEEEEEECCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCEEEECCEEECCCC
GFDKLNEMALKKGEKVFVNPRNAAAGSLRQLDSRITAKRPLAFYAYSVGVVQGAELSNSH
CHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHEECCCCCHHH
YQRFLQLKAWGLPMCPETKQLSSLEDVKAYYQDIMTRRDTLAYEIDGVVIKVDDIAAQET
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEECEEEEEECCHHHHHH
LGFVARAPRWAIAYKFPAQEEITLLNDVEFQVGRTGAITPVAKLEPIFVGGVTVSNATLH
HHHHHCCCCEEEEEECCCCCCEEEEECCEEEECCCCCCCCHHHCCEEEECCEEECCCCCC
NADEIARLGVKVGDSVIIRRAGDVIPQIVAVVQDRRPETAKDIVFPDACPVCNSAVERVE
CHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHC
GEAVARCTGGLVCQAQRKEALKHFVSRKALDVDGLGVKVIEQLVDREMVETPADLFKLSA
CCHHHHHCCCEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
GVITVLDRMGPKSAQNVVSALNKAKDTTLARFLYSLGIREVGEATAMNLAQHFKTLELVQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
AATHEQLVEVSDIGDIVASHLTSFFSQEKNRAVVDQLIELGVNWPAIEAVADDQELPLEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHEECCCCCCCCCC
KVVVLTGSLSKLGRSEAKAALQALGAKVTGSVSKKTDILFAGEAAGSKLTKAQDLGIEIR
EEEEEECCHHHHCHHHHHHHHHHHCCHHCCCCCCCCEEEEECCCCCCCCCHHHHCCEEEE
TEEDLIALIS
CCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA