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The map label for this gene is rnhA [H]

Identifier: 218710132

GI number: 218710132

Start: 2309511

End: 2310284

Strand: Reverse

Name: rnhA [H]

Synonym: VS_2153

Alternate gene names: 218710132

Gene position: 2310284-2309511 (Counterclockwise)

Preceding gene: 218710133

Following gene: 218710130

Centisome position: 70.02

GC content: 46.77

Gene sequence:

>774_bases
TTGGCTAAGAAGTATTATGTGGTTTGGAAAGGTCGTACACCGGGTATTTTTACTACTTGGAATGAGTGTAAGTCGCAAGT
TGATGGTTTTGCTGGTGCGAGATATAAATCGTTTCCAACACTAGGCGAGGCCGAGTCTGCTTTCGGTGGTAAAACGTCGT
CTGGGTCAGGTTCTTCGGCTACAAAGCCAAGCTCTGCGGGGAAGGCGACGAAAGCTAAGGTGCCGCCTCTTTCGGAGCAG
CAAATCACAGATATGCCTTTTGATATCAAGATCTTTACCGATGGTGCTTGTGAACCAAATCCTGGTGAAGCAGGGACGGG
GTTAGCGGTCTACCAAAACAACCAGTTAACAGAATTGTGGTATGGCTTGTATCAGCCTGTTGGTACCAACAATACCGCCG
AGTTACATGGGCTTAAGCAAGCGTTTGTTCTCGCGAAAGAGAAGTTAAACGCAGGCCTCTCTGTTGCGATTTATTGTGAC
TCTAAGTACTCGATAGATTGTATTACTAAGTGGGCAACGGGTTGGGAGAAAAAGGGCTGGACCAAGTCGGGCGGCGAGAT
CAAAAACCTCGATATTATTAAGCCTGCGTATGCTCTCTACCAAGAACTGGCTTCAAAAATCACCATCTACCATGTGAACG
GTCACGTTGGTATTGAAGGTAACGAATTGGCCGATAGAATGTCGATTGTGGCTATTTCATCGAAAGAGCAGGAGCTAGCT
CAATATCGTGACATCGAAGATCTTGAGTCTATTTTGGCACTTCGAGCCGGCTAG

Upstream 100 bases:

>100_bases
ACTTACGTGTTAGCAGAGTAGTCAGGCATAATAATGTGCCCATAGCTGAATCAGTTTTAGATGTGGATATTGTGTGTTTA
GGTTTTGGTAAGGAGTTAGT

Downstream 100 bases:

>100_bases
CTAGGCTTCTTAATGAAAAGCTAATAAAAGTTACTGAAAGCATAAGTTGCCATCATTAGGCTGTGCTAAGTTGAGCGTTA
CATTTCAAATCAATGTAGCG

Product: ribonuclease HI

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MAKKYYVVWKGRTPGIFTTWNECKSQVDGFAGARYKSFPTLGEAESAFGGKTSSGSGSSATKPSSAGKATKAKVPPLSEQ
QITDMPFDIKIFTDGACEPNPGEAGTGLAVYQNNQLTELWYGLYQPVGTNNTAELHGLKQAFVLAKEKLNAGLSVAIYCD
SKYSIDCITKWATGWEKKGWTKSGGEIKNLDIIKPAYALYQELASKITIYHVNGHVGIEGNELADRMSIVAISSKEQELA
QYRDIEDLESILALRAG

Sequences:

>Translated_257_residues
MAKKYYVVWKGRTPGIFTTWNECKSQVDGFAGARYKSFPTLGEAESAFGGKTSSGSGSSATKPSSAGKATKAKVPPLSEQ
QITDMPFDIKIFTDGACEPNPGEAGTGLAVYQNNQLTELWYGLYQPVGTNNTAELHGLKQAFVLAKEKLNAGLSVAIYCD
SKYSIDCITKWATGWEKKGWTKSGGEIKNLDIIKPAYALYQELASKITIYHVNGHVGIEGNELADRMSIVAISSKEQELA
QYRDIEDLESILALRAG
>Mature_256_residues
AKKYYVVWKGRTPGIFTTWNECKSQVDGFAGARYKSFPTLGEAESAFGGKTSSGSGSSATKPSSAGKATKAKVPPLSEQQ
ITDMPFDIKIFTDGACEPNPGEAGTGLAVYQNNQLTELWYGLYQPVGTNNTAELHGLKQAFVLAKEKLNAGLSVAIYCDS
KYSIDCITKWATGWEKKGWTKSGGEIKNLDIIKPAYALYQELASKITIYHVNGHVGIEGNELADRMSIVAISSKEQELAQ
YRDIEDLESILALRAG

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG3341

COG function: function code R; Predicted double-stranded RNA/RNA-DNA hybrid binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=257, Percent_Identity=32.6848249027237, Blast_Score=107, Evalue=9e-24,
Organism=Escherichia coli, GI1786408, Length=154, Percent_Identity=31.8181818181818, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI115532542, Length=240, Percent_Identity=37.0833333333333, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6323890, Length=86, Percent_Identity=43.0232558139535, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45552509, Length=297, Percent_Identity=28.6195286195286, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17137476, Length=167, Percent_Identity=34.1317365269461, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 27895; Mature: 27764

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKYYVVWKGRTPGIFTTWNECKSQVDGFAGARYKSFPTLGEAESAFGGKTSSGSGSSA
CCCEEEEEEECCCCCEEECHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCC
TKPSSAGKATKAKVPPLSEQQITDMPFDIKIFTDGACEPNPGEAGTGLAVYQNNQLTELW
CCCCCCCCCCCCCCCCCCCCHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCHHHHH
YGLYQPVGTNNTAELHGLKQAFVLAKEKLNAGLSVAIYCDSKYSIDCITKWATGWEKKGW
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEHHCCCCCCCCC
TKSGGEIKNLDIIKPAYALYQELASKITIYHVNGHVGIEGNELADRMSIVAISSKEQELA
CCCCCCCCCEEEECHHHHHHHHHHCCEEEEEECCEEECCCHHHHHHEEEEEECCCHHHHH
QYRDIEDLESILALRAG
HHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AKKYYVVWKGRTPGIFTTWNECKSQVDGFAGARYKSFPTLGEAESAFGGKTSSGSGSSA
CCEEEEEEECCCCCEEECHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCC
TKPSSAGKATKAKVPPLSEQQITDMPFDIKIFTDGACEPNPGEAGTGLAVYQNNQLTELW
CCCCCCCCCCCCCCCCCCCCHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCHHHHH
YGLYQPVGTNNTAELHGLKQAFVLAKEKLNAGLSVAIYCDSKYSIDCITKWATGWEKKGW
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEHHCCCCCCCCC
TKSGGEIKNLDIIKPAYALYQELASKITIYHVNGHVGIEGNELADRMSIVAISSKEQELA
CCCCCCCCCEEEECHHHHHHHHHHCCEEEEEECCEEECCCHHHHHHEEEEEECCCHHHHH
QYRDIEDLESILALRAG
HHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA