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The map label for this gene is dmlR [H]

Identifier: 218710114

GI number: 218710114

Start: 2291846

End: 2292805

Strand: Reverse

Name: dmlR [H]

Synonym: VS_2135

Alternate gene names: 218710114

Gene position: 2292805-2291846 (Counterclockwise)

Preceding gene: 218710116

Following gene: 218710113

Centisome position: 69.49

GC content: 44.27

Gene sequence:

>960_bases
ATGGACATATCGAGTCGTTTATTATTGTTACTTGAAGTGGTTGAGCTGGGTTCGTTTGTCAAAGTGGCGGAGCAAAGAAA
CGTTGACCGTTCTGTTATCTCTAAACAGATCAGCCGTCTTGAAGAAGAGCTGGATGTTCGGTTACTCAATCGCACTACTC
GTTCATTATCTTTGACAGCTGCAGGCAATGAGATGGTCAATCAAGCGCATCAGCTTCGTGCACTGTTGAACGATACTCGA
CGCTTGGCGCAAAACTATCATGCAGAGCCTCGAGGGTTATTAAGAATCACCAGCTCTACTATGTTTGGTCGACAATATGT
TCAGCAGGCGATTGCGGTTTTCCAGAAGCAGTATCCAGATGTTGATTTTGAATTGAGACTCGAAGACCGAATCGTCGATA
TGGTTAAAGAAGGGTTTGATATTGGTTTCCGTATTGGTAAACCCAAAAACTCCAGCCTAATCAGCCGTAAGATAGCCAGA
AGTCGATTGTTGATTGTCGCTTCACCTGAGTTCATTGCTCAGCATGGCGAGATCAACACCATCGAAAAGTTAGAGTCATT
ACCTGCGACTATTTACGCTGCTCCGGGTTTGTTGATTGATAAGTTTTCTTATGTTGATCAAGAGGGGCAAGCGCAGCATT
TTCAGCTTAATGCCGCTTATAAAGTGAATGACGTTGAGATGATTTCCAAGAGCGCGCTAACTGGCAGTACTCTCGCGGTT
GTCACGGCGCAGATGATTGAAGATGAAGTGCTCAGTGGAGAGTTGGTGCCTATTATGACTCACTTAAACTTGGATGACTT
TGGCACTTTCTATGCGGTGTACCCACATCGAGATGCACCCATAAAAACTAAGCTCTTCATTGATACATTGAAAACTATTG
TCGGTGAAGATAAACCCGTGTGGGAGCAAGGCATTCCTGGGTTTGACGACATGTATGGTAATCAGCGAACAAAATCATAG

Upstream 100 bases:

>100_bases
TGTTGTCTCCGTTATGTGCAGCTACTATAGATTTGTAATAAGATGAGAAAAATACTCATATTGGCACTTAATTGGTGCTT
TAAATGCAACAAAGGGCGTT

Downstream 100 bases:

>100_bases
TTCAAAAAATCAATACGTTAGGTTGCTTTGTTGCGCGTATTTTTTAATCTAGCCCAAACCTAAGTTTGAACCCAAACTTC
AAGTTAAAGTGAACTCTATT

Product: hypothetical protein

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MDISSRLLLLLEVVELGSFVKVAEQRNVDRSVISKQISRLEEELDVRLLNRTTRSLSLTAAGNEMVNQAHQLRALLNDTR
RLAQNYHAEPRGLLRITSSTMFGRQYVQQAIAVFQKQYPDVDFELRLEDRIVDMVKEGFDIGFRIGKPKNSSLISRKIAR
SRLLIVASPEFIAQHGEINTIEKLESLPATIYAAPGLLIDKFSYVDQEGQAQHFQLNAAYKVNDVEMISKSALTGSTLAV
VTAQMIEDEVLSGELVPIMTHLNLDDFGTFYAVYPHRDAPIKTKLFIDTLKTIVGEDKPVWEQGIPGFDDMYGNQRTKS

Sequences:

>Translated_319_residues
MDISSRLLLLLEVVELGSFVKVAEQRNVDRSVISKQISRLEEELDVRLLNRTTRSLSLTAAGNEMVNQAHQLRALLNDTR
RLAQNYHAEPRGLLRITSSTMFGRQYVQQAIAVFQKQYPDVDFELRLEDRIVDMVKEGFDIGFRIGKPKNSSLISRKIAR
SRLLIVASPEFIAQHGEINTIEKLESLPATIYAAPGLLIDKFSYVDQEGQAQHFQLNAAYKVNDVEMISKSALTGSTLAV
VTAQMIEDEVLSGELVPIMTHLNLDDFGTFYAVYPHRDAPIKTKLFIDTLKTIVGEDKPVWEQGIPGFDDMYGNQRTKS
>Mature_319_residues
MDISSRLLLLLEVVELGSFVKVAEQRNVDRSVISKQISRLEEELDVRLLNRTTRSLSLTAAGNEMVNQAHQLRALLNDTR
RLAQNYHAEPRGLLRITSSTMFGRQYVQQAIAVFQKQYPDVDFELRLEDRIVDMVKEGFDIGFRIGKPKNSSLISRKIAR
SRLLIVASPEFIAQHGEINTIEKLESLPATIYAAPGLLIDKFSYVDQEGQAQHFQLNAAYKVNDVEMISKSALTGSTLAV
VTAQMIEDEVLSGELVPIMTHLNLDDFGTFYAVYPHRDAPIKTKLFIDTLKTIVGEDKPVWEQGIPGFDDMYGNQRTKS

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=301, Percent_Identity=30.8970099667774, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=30.7958477508651, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1789639, Length=292, Percent_Identity=27.3972602739726, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787128, Length=288, Percent_Identity=26.0416666666667, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1787589, Length=292, Percent_Identity=26.027397260274, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI145693193, Length=298, Percent_Identity=24.8322147651007, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1789440, Length=186, Percent_Identity=26.8817204301075, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1787879, Length=149, Percent_Identity=32.8859060402685, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788706, Length=287, Percent_Identity=23.3449477351916, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789173, Length=244, Percent_Identity=25.4098360655738, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082132, Length=175, Percent_Identity=27.4285714285714, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788748, Length=142, Percent_Identity=29.5774647887324, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI157672245, Length=180, Percent_Identity=28.8888888888889, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786448, Length=264, Percent_Identity=24.6212121212121, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788481, Length=263, Percent_Identity=25.0950570342205, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35971; Mature: 35971

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDISSRLLLLLEVVELGSFVKVAEQRNVDRSVISKQISRLEEELDVRLLNRTTRSLSLTA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
AGNEMVNQAHQLRALLNDTRRLAQNYHAEPRGLLRITSSTMFGRQYVQQAIAVFQKQYPD
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCC
VDFELRLEDRIVDMVKEGFDIGFRIGKPKNSSLISRKIARSRLLIVASPEFIAQHGEINT
CCEEEEHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCEEEEEECHHHHHHCCCCHH
IEKLESLPATIYAAPGLLIDKFSYVDQEGQAQHFQLNAAYKVNDVEMISKSALTGSTLAV
HHHHHHCCCEEEECCCHHHHHHHHCCCCCCCEEEEEECEEEECHHHHHHHHHCCCCHHHH
VTAQMIEDEVLSGELVPIMTHLNLDDFGTFYAVYPHRDAPIKTKLFIDTLKTIVGEDKPV
HHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCC
WEQGIPGFDDMYGNQRTKS
HHCCCCCCHHHCCCCCCCC
>Mature Secondary Structure
MDISSRLLLLLEVVELGSFVKVAEQRNVDRSVISKQISRLEEELDVRLLNRTTRSLSLTA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
AGNEMVNQAHQLRALLNDTRRLAQNYHAEPRGLLRITSSTMFGRQYVQQAIAVFQKQYPD
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCC
VDFELRLEDRIVDMVKEGFDIGFRIGKPKNSSLISRKIARSRLLIVASPEFIAQHGEINT
CCEEEEHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCEEEEEECHHHHHHCCCCHH
IEKLESLPATIYAAPGLLIDKFSYVDQEGQAQHFQLNAAYKVNDVEMISKSALTGSTLAV
HHHHHHCCCEEEECCCHHHHHHHHCCCCCCCEEEEEECEEEECHHHHHHHHHCCCCHHHH
VTAQMIEDEVLSGELVPIMTHLNLDDFGTFYAVYPHRDAPIKTKLFIDTLKTIVGEDKPV
HHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCC
WEQGIPGFDDMYGNQRTKS
HHCCCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]