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The map label for this gene is rhtB [H]

Identifier: 218710107

GI number: 218710107

Start: 2288377

End: 2288976

Strand: Reverse

Name: rhtB [H]

Synonym: VS_2128

Alternate gene names: 218710107

Gene position: 2288976-2288377 (Counterclockwise)

Preceding gene: 218710109

Following gene: 218710104

Centisome position: 69.38

GC content: 45.17

Gene sequence:

>600_bases
ATGAGCTCTCAATTAATGCTGGCGTTCTTGCTATTTTCAACATCAATTGCAATTACACCTGGGGCAGGCAATATCGCATT
ACTTGGGATTTCAAGTCGTTATGGATTTGCTGCGACTTTACCTTTTATCTCTGGGAACGCTGTTGGCATCATCATCGTAC
TGGCAGGATCCAGTGTCGGATTGGTGAGTCTATTTACCCTTTACCCAGATCTTTATAACATTTTGAAATACGCGGGTGCG
GCTTACTTGCTGTTTATGGCGTGGTCGATTGCGAACATGCAAATCGAAGAAAGCACGACTGATAATCGCTCGGGCTTTAT
GTCAGGCGTGTTAGTGCAGGTGTTGAACCCTAAAGGTTGGATTGCTTCATTAACCGTGTTTTCGCAGTTCATCACCCCGA
ATGCAGATTACTTGATCCAAGTAGTGACTATTATCGCGGGTATGGTTATTACCGGTGTGCCGTGCATGTTGGTGTGGGCG
TATTGCGGCACCATGCTGAAAAAATTACTGCAATCACCAAAACAGATGATGTTTGTGAACCGTTGCTTGGGTGGAAGCTT
AGCGATGGTGGTTGCCTTTATGCTTTATCAACCGGCATAA

Upstream 100 bases:

>100_bases
TCAAATTTGCATTAATATTAGCCTATATAGCAATTTAATTCCTAGTCGAATCTGTAAACTCTCTCCGTATAAATACTTTA
CTTATTAGGAGTGAGAGGAA

Downstream 100 bases:

>100_bases
ATCACAAAGAAAAAAACCACTCCTAAATCCATAGGAGTGGTGTCTAAATGTCGACTAAGTCGTTAACAGTAAAGAATTAA
GTTAACAAATATTTAAGGAA

Product: putative threonine efflux protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MSSQLMLAFLLFSTSIAITPGAGNIALLGISSRYGFAATLPFISGNAVGIIIVLAGSSVGLVSLFTLYPDLYNILKYAGA
AYLLFMAWSIANMQIEESTTDNRSGFMSGVLVQVLNPKGWIASLTVFSQFITPNADYLIQVVTIIAGMVITGVPCMLVWA
YCGTMLKKLLQSPKQMMFVNRCLGGSLAMVVAFMLYQPA

Sequences:

>Translated_199_residues
MSSQLMLAFLLFSTSIAITPGAGNIALLGISSRYGFAATLPFISGNAVGIIIVLAGSSVGLVSLFTLYPDLYNILKYAGA
AYLLFMAWSIANMQIEESTTDNRSGFMSGVLVQVLNPKGWIASLTVFSQFITPNADYLIQVVTIIAGMVITGVPCMLVWA
YCGTMLKKLLQSPKQMMFVNRCLGGSLAMVVAFMLYQPA
>Mature_198_residues
SSQLMLAFLLFSTSIAITPGAGNIALLGISSRYGFAATLPFISGNAVGIIIVLAGSSVGLVSLFTLYPDLYNILKYAGAA
YLLFMAWSIANMQIEESTTDNRSGFMSGVLVQVLNPKGWIASLTVFSQFITPNADYLIQVVTIIAGMVITGVPCMLVWAY
CGTMLKKLLQSPKQMMFVNRCLGGSLAMVVAFMLYQPA

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=201, Percent_Identity=29.3532338308458, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1788932, Length=175, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21336; Mature: 21205

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSQLMLAFLLFSTSIAITPGAGNIALLGISSRYGFAATLPFISGNAVGIIIVLAGSSVG
CCCHHHHHHHHHHCCEEEECCCCCEEEEEECCCCCCEEECCCCCCCCEEEEEEEECCCCH
LVSLFTLYPDLYNILKYAGAAYLLFMAWSIANMQIEESTTDNRSGFMSGVLVQVLNPKGW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHHCCCCCH
IASLTVFSQFITPNADYLIQVVTIIAGMVITGVPCMLVWAYCGTMLKKLLQSPKQMMFVN
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCHHHHHHHH
RCLGGSLAMVVAFMLYQPA
HHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSQLMLAFLLFSTSIAITPGAGNIALLGISSRYGFAATLPFISGNAVGIIIVLAGSSVG
CCHHHHHHHHHHCCEEEECCCCCEEEEEECCCCCCEEECCCCCCCCEEEEEEEECCCCH
LVSLFTLYPDLYNILKYAGAAYLLFMAWSIANMQIEESTTDNRSGFMSGVLVQVLNPKGW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHHCCCCCH
IASLTVFSQFITPNADYLIQVVTIIAGMVITGVPCMLVWAYCGTMLKKLLQSPKQMMFVN
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCHHHHHHHH
RCLGGSLAMVVAFMLYQPA
HHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]