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The map label for this gene is miaE [H]

Identifier: 218710032

GI number: 218710032

Start: 2202811

End: 2203605

Strand: Direct

Name: miaE [H]

Synonym: VS_2052

Alternate gene names: 218710032

Gene position: 2202811-2203605 (Clockwise)

Preceding gene: 218710031

Following gene: 218710044

Centisome position: 66.77

GC content: 41.01

Gene sequence:

>795_bases
GTGATAAAATCCCGCATTATCAACAAACTTGCCCTTACCATGTATCAAGAATTATTAGCCCCTATACACTCTTTTCTTAA
AGCTGAAACGCCAGATTCATGGATAGATGAAGCCAAGAAGCCTGAAAACCTTCATATCATTCTACGCGATCACATGCTTT
GTGAACTTAAAGCAGCTCAAAGTGCTTTGTTCCTTATCCGTAAATATGCGGTAGATAAAGAAAGCGCGAAGCAACTGAAT
GAGTGGATTTTGCCCTACGAGCAATTTGCTTACCGTCGTATTGGAGACTTGGAGTCTCTACGTGGTAAAAGTAATGTATC
AAAGCAAATTACAGCGAAAGAAGGCTGTAATTACGGTGCTGACTTGATCGATAAAATGGTATTGCTGATCAAAGAAGAGC
TGCATCACTTCTACCAAGTGATGGAATTGATGGTAAAAAAAGGCGTGACTTATCAACCGATCGAGGCGGGCCGCTACGCT
AAAGGTTTAATCAAGCAAGTTAAAACTTACGAACCTGATGCTCTGGTTGATAAGCTTATTATTGGTGCATTTATTGAAGC
TCGCTCTTGTGAACGCTTTGCTAAACTGGCTCCTTTCTTAGAAGAAGACATGGAAAAGTTTTACGTATCTCTACTTCGTT
CAGAAGCTCGTCACTATCAAGACTATCTTGAACTCGCGGAACAAATCGCAGGGAAAGATATCTCTGAGCGTATTGCTCAT
TTTGCGCAAGTTGAAGCAGAACTTATCACTTCAGAAGACAGCGACTTTAAGTTTCATAGTGGCGCTCCGGTTTAA

Upstream 100 bases:

>100_bases
AACGCTCTAACTCAACACTTAAAGGCCGCTCTATTTAAATTAGCGGCCTTTTTTGTACCTGTGGAATTTCCTTTCTCCGT
ATAGGGGGATCTCTTAAGCT

Downstream 100 bases:

>100_bases
GCCGTTTAGAACAAGTCTACTCCAGACCAATTCTGTATTTAGTACACCTATAAGAAGGCCAGCAATTTGCTGGCCTTCTT
TATTTATATCGCGTTCTCAA

Product: tRNA-(MS[2]IO[6]A)-hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MIKSRIINKLALTMYQELLAPIHSFLKAETPDSWIDEAKKPENLHIILRDHMLCELKAAQSALFLIRKYAVDKESAKQLN
EWILPYEQFAYRRIGDLESLRGKSNVSKQITAKEGCNYGADLIDKMVLLIKEELHHFYQVMELMVKKGVTYQPIEAGRYA
KGLIKQVKTYEPDALVDKLIIGAFIEARSCERFAKLAPFLEEDMEKFYVSLLRSEARHYQDYLELAEQIAGKDISERIAH
FAQVEAELITSEDSDFKFHSGAPV

Sequences:

>Translated_264_residues
MIKSRIINKLALTMYQELLAPIHSFLKAETPDSWIDEAKKPENLHIILRDHMLCELKAAQSALFLIRKYAVDKESAKQLN
EWILPYEQFAYRRIGDLESLRGKSNVSKQITAKEGCNYGADLIDKMVLLIKEELHHFYQVMELMVKKGVTYQPIEAGRYA
KGLIKQVKTYEPDALVDKLIIGAFIEARSCERFAKLAPFLEEDMEKFYVSLLRSEARHYQDYLELAEQIAGKDISERIAH
FAQVEAELITSEDSDFKFHSGAPV
>Mature_264_residues
MIKSRIINKLALTMYQELLAPIHSFLKAETPDSWIDEAKKPENLHIILRDHMLCELKAAQSALFLIRKYAVDKESAKQLN
EWILPYEQFAYRRIGDLESLRGKSNVSKQITAKEGCNYGADLIDKMVLLIKEELHHFYQVMELMVKKGVTYQPIEAGRYA
KGLIKQVKTYEPDALVDKLIIGAFIEARSCERFAKLAPFLEEDMEKFYVSLLRSEARHYQDYLELAEQIAGKDISERIAH
FAQVEAELITSEDSDFKFHSGAPV

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 30404; Mature: 30404

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKSRIINKLALTMYQELLAPIHSFLKAETPDSWIDEAKKPENLHIILRDHMLCELKAAQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEHHHHHHHHHHHH
SALFLIRKYAVDKESAKQLNEWILPYEQFAYRRIGDLESLRGKSNVSKQITAKEGCNYGA
HHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHCCCCCHHHHHHHHHCCCCCH
DLIDKMVLLIKEELHHFYQVMELMVKKGVTYQPIEAGRYAKGLIKQVKTYEPDALVDKLI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
IGAFIEARSCERFAKLAPFLEEDMEKFYVSLLRSEARHYQDYLELAEQIAGKDISERIAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
FAQVEAELITSEDSDFKFHSGAPV
HHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MIKSRIINKLALTMYQELLAPIHSFLKAETPDSWIDEAKKPENLHIILRDHMLCELKAAQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEHHHHHHHHHHHH
SALFLIRKYAVDKESAKQLNEWILPYEQFAYRRIGDLESLRGKSNVSKQITAKEGCNYGA
HHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHCCCCCHHHHHHHHHCCCCCH
DLIDKMVLLIKEELHHFYQVMELMVKKGVTYQPIEAGRYAKGLIKQVKTYEPDALVDKLI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
IGAFIEARSCERFAKLAPFLEEDMEKFYVSLLRSEARHYQDYLELAEQIAGKDISERIAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
FAQVEAELITSEDSDFKFHSGAPV
HHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]