Click here to switch to the map view.

The map label for this gene is hns [H]

Identifier: 218710009

GI number: 218710009

Start: 2175551

End: 2175961

Strand: Direct

Name: hns [H]

Synonym: VS_2029

Alternate gene names: 218710009

Gene position: 2175551-2175961 (Clockwise)

Preceding gene: 218709999

Following gene: 218710012

Centisome position: 65.94

GC content: 42.09

Gene sequence:

>411_bases
ATGTCTGAATTAACAAAAACTCTATTAAATATCCGTAGCCTTCGTGCGTTCTCACGTGAATTAACTCTTGAGCAACTTGA
AGAAGCGCTAGACAAGCTGACTATTGTTGTACAAGAACGTCAAGAGTCTGAAGCTGAAGAACGCGCAGCAAAAGCAGAGC
AAGAAGCTAAACTTTCTGCTATCGCAGAACAAATTGCAAAAGACGGAATCGATGTTGCCGATCTTATTGCTGCACTTTCT
GGTGAAGCAAAATCTAAAACAACAAAATCTAAACGTGCTCCTCGCCCTGCGAAATACAAATACGTAGACGCGAATGGCGA
CGAAAAAACTTGGACAGGTCAAGGCCGTACGCCTTCAGCTATCCAAGAACAACTAGATGCTGGTAAATCTCTAGAAGAGT
TTGCTCTTTAA

Upstream 100 bases:

>100_bases
TTTTTATTTAAATAACTAATAGAAGAAATACTATTTGTTTTATTCTTAGCCTCTGTTTATTATTAGGAGGATTATTTAGT
TTACCTTGATGGGAAATATC

Downstream 100 bases:

>100_bases
GGCATCGAGTTTGCTAAGTGAATAATCAATAACCCACTAAGCAATAGAATTAATATTTAAGTCGACTAAAGTATTATTTA
CGGCTTCAATATATTCAGAA

Product: DNA-binding protein H-NS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 136; Mature: 135

Protein sequence:

>136_residues
MSELTKTLLNIRSLRAFSRELTLEQLEEALDKLTIVVQERQESEAEERAAKAEQEAKLSAIAEQIAKDGIDVADLIAALS
GEAKSKTTKSKRAPRPAKYKYVDANGDEKTWTGQGRTPSAIQEQLDAGKSLEEFAL

Sequences:

>Translated_136_residues
MSELTKTLLNIRSLRAFSRELTLEQLEEALDKLTIVVQERQESEAEERAAKAEQEAKLSAIAEQIAKDGIDVADLIAALS
GEAKSKTTKSKRAPRPAKYKYVDANGDEKTWTGQGRTPSAIQEQLDAGKSLEEFAL
>Mature_135_residues
SELTKTLLNIRSLRAFSRELTLEQLEEALDKLTIVVQERQESEAEERAAKAEQEAKLSAIAEQIAKDGIDVADLIAALSG
EAKSKTTKSKRAPRPAKYKYVDANGDEKTWTGQGRTPSAIQEQLDAGKSLEEFAL

Specific function: Binds tightly to ds-DNA, increases its thermal stability and inhibits transcription [H]

COG id: COG2916

COG function: function code R; DNA-binding protein H-NS

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the histone-like protein H-NS family [H]

Homologues:

Organism=Escherichia coli, GI1787489, Length=137, Percent_Identity=52.5547445255474, Blast_Score=117, Evalue=3e-28,
Organism=Escherichia coli, GI1789023, Length=136, Percent_Identity=44.1176470588235, Blast_Score=109, Evalue=8e-26,

Paralogues:

None

Copy number: 820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1780 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 1020 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 4632 Molecules/Cell In: Gr

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001801 [H]

Pfam domain/function: PF00816 Histone_HNS [H]

EC number: NA

Molecular weight: Translated: 14977; Mature: 14846

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELTKTLLNIRSLRAFSRELTLEQLEEALDKLTIVVQERQESEAEERAAKAEQEAKLSA
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAEQIAKDGIDVADLIAALSGEAKSKTTKSKRAPRPAKYKYVDANGDEKTWTGQGRTPSA
HHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCHHH
IQEQLDAGKSLEEFAL
HHHHHHCCCCHHHHCC
>Mature Secondary Structure 
SELTKTLLNIRSLRAFSRELTLEQLEEALDKLTIVVQERQESEAEERAAKAEQEAKLSA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAEQIAKDGIDVADLIAALSGEAKSKTTKSKRAPRPAKYKYVDANGDEKTWTGQGRTPSA
HHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCHHH
IQEQLDAGKSLEEFAL
HHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]