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The map label for this gene is pspA [H]

Identifier: 218709972

GI number: 218709972

Start: 2138059

End: 2138730

Strand: Reverse

Name: pspA [H]

Synonym: VS_1991

Alternate gene names: 218709972

Gene position: 2138730-2138059 (Counterclockwise)

Preceding gene: 218709981

Following gene: 218709971

Centisome position: 64.82

GC content: 40.92

Gene sequence:

>672_bases
ATGGGTATTTTTTCTCGCTTTGCAGACATTGTAAATTCAAACATCAGTGCACTATTAGATAAGGCTGAAGATCCTGAAAA
GATGATTCGCCTCATTATTCAAGAAATGGAAGACACGCTGGTTGAGGTTCGTACTAACTCAGCAAAAGCCATTGCAGACA
AGAAAGAGCTAGCACGTAAAGTTGAAGCTATCGAAACTCAGATTCTAGATTGGCAGAATAAAGCGACATTAGCCCTGACT
AAACAACGTGAAGATCTGGCTAGAGCGGCACTAATCGAAAAGCAAAAACTTGAAGATATCATCAAGAGTCTTCATACCGA
GCAAACCTTGGTTCATGAAACCATCGAAAAACTAACCAGTGAGATCGGTAAGCTTGAAACCAAAATCGCCGAGACTCGTG
CAAAACAGCAAGCACTAATGATTCGTAATAATGCAGCAAGCAATCGTCGCGATGTTCAAAAACATCTGCATTCAAGCAAA
ACCAACGAAGCAATGGCGAAGTTTGAGCAATTCTCACGTAAAGTGGATGAATTAGAAGCTGAAGCTGACGTTTACGCAAA
AACCGGTAACGCAAAATCTTTAGACCAAGAGTTTGCAGAGCTACAAGCTCAAGACGAGATTGAAAAAGAGCTGGCGAAAC
TGAAGCAACAAGTTGAAAACCGCGATAAATAA

Upstream 100 bases:

>100_bases
TGGTAGGTTTAACAACATACGTAGATTTTTGAGCAAGTGGATTGAGAAATCGAAAACGATTTAAAACCTATTTGCCATTA
TTGCAATTAAGGAGTTTCTT

Downstream 100 bases:

>100_bases
TTTTAGGAGTTGTCTATGTCAACATTTCTAATTGCAGGTCCACTGATTGTCTTTTTGATCTTCGTGGCACCGCTATGGTT
ATTCTTACACTACCGCAGCA

Product: phage shock protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MGIFSRFADIVNSNISALLDKAEDPEKMIRLIIQEMEDTLVEVRTNSAKAIADKKELARKVEAIETQILDWQNKATLALT
KQREDLARAALIEKQKLEDIIKSLHTEQTLVHETIEKLTSEIGKLETKIAETRAKQQALMIRNNAASNRRDVQKHLHSSK
TNEAMAKFEQFSRKVDELEAEADVYAKTGNAKSLDQEFAELQAQDEIEKELAKLKQQVENRDK

Sequences:

>Translated_223_residues
MGIFSRFADIVNSNISALLDKAEDPEKMIRLIIQEMEDTLVEVRTNSAKAIADKKELARKVEAIETQILDWQNKATLALT
KQREDLARAALIEKQKLEDIIKSLHTEQTLVHETIEKLTSEIGKLETKIAETRAKQQALMIRNNAASNRRDVQKHLHSSK
TNEAMAKFEQFSRKVDELEAEADVYAKTGNAKSLDQEFAELQAQDEIEKELAKLKQQVENRDK
>Mature_222_residues
GIFSRFADIVNSNISALLDKAEDPEKMIRLIIQEMEDTLVEVRTNSAKAIADKKELARKVEAIETQILDWQNKATLALTK
QREDLARAALIEKQKLEDIIKSLHTEQTLVHETIEKLTSEIGKLETKIAETRAKQQALMIRNNAASNRRDVQKHLHSSKT
NEAMAKFEQFSRKVDELEAEADVYAKTGNAKSLDQEFAELQAQDEIEKELAKLKQQVENRDK

Specific function: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspA negatively regulates expression of the pspABCDE promoter and of pspG through negative regulation

COG id: COG1842

COG function: function code KT; Phage shock protein A (IM30), suppresses sigma54-dependent transcription

Gene ontology:

Cell location: Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PspA/IM30 family [H]

Homologues:

Organism=Escherichia coli, GI2367118, Length=215, Percent_Identity=61.3953488372093, Blast_Score=264, Evalue=5e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014319
- InterPro:   IPR007157 [H]

Pfam domain/function: PF04012 PspA_IM30 [H]

EC number: NA

Molecular weight: Translated: 25441; Mature: 25310

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIFSRFADIVNSNISALLDKAEDPEKMIRLIIQEMEDTLVEVRTNSAKAIADKKELARK
CCHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VEAIETQILDWQNKATLALTKQREDLARAALIEKQKLEDIIKSLHTEQTLVHETIEKLTS
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIGKLETKIAETRAKQQALMIRNNAASNRRDVQKHLHSSKTNEAMAKFEQFSRKVDELEA
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
EADVYAKTGNAKSLDQEFAELQAQDEIEKELAKLKQQVENRDK
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GIFSRFADIVNSNISALLDKAEDPEKMIRLIIQEMEDTLVEVRTNSAKAIADKKELARK
CHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VEAIETQILDWQNKATLALTKQREDLARAALIEKQKLEDIIKSLHTEQTLVHETIEKLTS
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIGKLETKIAETRAKQQALMIRNNAASNRRDVQKHLHSSKTNEAMAKFEQFSRKVDELEA
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
EADVYAKTGNAKSLDQEFAELQAQDEIEKELAKLKQQVENRDK
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]