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The map label for this gene is cls [H]

Identifier: 218709962

GI number: 218709962

Start: 2126899

End: 2128353

Strand: Direct

Name: cls [H]

Synonym: VS_1981

Alternate gene names: 218709962

Gene position: 2126899-2128353 (Clockwise)

Preceding gene: 218709957

Following gene: 218709963

Centisome position: 64.47

GC content: 45.7

Gene sequence:

>1455_bases
GTGGAAAAGCTCTACCATTTTTTAACTTTAGCTTCTGTCGCTTTATACTGGGTCCTTGTCGCCGGTGTGACTTTTCGTGT
CGTACTAAAACGACGCTCTGTCAGCGTTTCTCTCGCATGGTTGATGGTTATCTACATTATCCCTATTGTTGGCGTCGCTT
GTTACTTCCTTTTCGGAGAGCTAAATCTTGGTAGAAAGAGGGCTGAGCGGGCACATCGCATGTTTACGCCTTTTGGTGAT
TGGTTCCGCCAATTAAATGATTGCCAAGTCCACCTTCCAGGTAAAGTCGGCTCGCCTATCCACAAAATTGATGAGCTTTG
TAACAATCGGATGGGCATTCCAGCGCTCAGCGGAAACACACTTTCACTGCAGGCTTCTCCTAATGAGATCCTACACGCAA
TTATCGAAGATATAGAAAACGCCCAGACTAGTATCAAAATGGTTTTCTATATTTGGCACCCCGGCGGCTTAACTGACTCC
GTTGCTTCCGCACTTATTCGAGCAGCAAAACGTGGTGTCGATGTTAAAGTTTTACTCGATTCTGCAGGTAGCCCGCGCTT
TTTCAAAAGTCACTGGCGTTCAATGATGAAAGACGCTGGCGTTCACGTTGTCCAAGCATTAGAGGTTAGTCCTTGGCGTA
TTTTCTTACGCCGTTTAGATCTAAGGCAGCACCGGAAGATCATCGTGATTGATGAGTCCATCGCCTATACCGGGTCAATG
AATATGGTAGATCCAGCTCATTTCAAACAGAGCTCAGGTGTCGGCCAATGGATTGATGTCATGGTTCGCGTCACTGGGCC
AACCGTTAACGTGTTGTCAGCAATTCATGGTTGGGATTGGGAAGTGGAAACCGGTGAACGCATTCTTCCTGATTTACCAG
AGTGCCCGGTTGAAGAAATCATGACGCATCATCCAGTTCAAGTAGTGCCATCTGGGCCGGGCATGCCGGAGTATTTGATT
CTGCAAGTTCTTACTATTGCGATTAATCAAGCAAACCATTCAGTTCGTATTACAACACCCTACTTTGTACCGAGTGCCGA
CTTATTAGAAACACTCAAAATGACGGCACAACGTGGTGTCAGCGTAGAATTAATCATTCCACATAACAACGATTCATTAA
TGGTTAAATGGGCTTCTCGCGCCTTCTATACCGAACTGCTTGAAGCCGGAGTGAAGATATATGAGTTTTACGGCGGGCTT
CTTCACACCAAATCGGTAGTGATTGATGAAGAGTTTTGCTTAATCGGAACGGTCAATATCGACATGCGCAGCCTATGGCT
TAACTTCGAAGTAACGCTAGCCGTTGATGATGTGCACTTCACCAAGCAACTGTTTGATTTGCAGCAATCCTATATTGAAT
CATCTCATCCAGTGGAATTGGAGCAATGGGAGCAGAGAAACCTACGCAACCGCTTCTTCGAAAGGTTGTTTTACTTATTC
AACCCGCTGCTTTAA

Upstream 100 bases:

>100_bases
TTACAGCTTTTGCTCTATTTTGTAAATAGTTGATTCCTTAAATTCCCATATCCGTAAAATAGCATTACTATCTAAGTGTC
TCTTTTTTCGAGTATTCAAT

Downstream 100 bases:

>100_bases
TTCCATCAATGCGTCCACCGCGGCTCATCACTCGCCGACTATCGGATTTTAAACCTTTCACGATAAACGACGTGGAAGGT
TAAAAAGCAAAAGAAAAGCG

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MEKLYHFLTLASVALYWVLVAGVTFRVVLKRRSVSVSLAWLMVIYIIPIVGVACYFLFGELNLGRKRAERAHRMFTPFGD
WFRQLNDCQVHLPGKVGSPIHKIDELCNNRMGIPALSGNTLSLQASPNEILHAIIEDIENAQTSIKMVFYIWHPGGLTDS
VASALIRAAKRGVDVKVLLDSAGSPRFFKSHWRSMMKDAGVHVVQALEVSPWRIFLRRLDLRQHRKIIVIDESIAYTGSM
NMVDPAHFKQSSGVGQWIDVMVRVTGPTVNVLSAIHGWDWEVETGERILPDLPECPVEEIMTHHPVQVVPSGPGMPEYLI
LQVLTIAINQANHSVRITTPYFVPSADLLETLKMTAQRGVSVELIIPHNNDSLMVKWASRAFYTELLEAGVKIYEFYGGL
LHTKSVVIDEEFCLIGTVNIDMRSLWLNFEVTLAVDDVHFTKQLFDLQQSYIESSHPVELEQWEQRNLRNRFFERLFYLF
NPLL

Sequences:

>Translated_484_residues
MEKLYHFLTLASVALYWVLVAGVTFRVVLKRRSVSVSLAWLMVIYIIPIVGVACYFLFGELNLGRKRAERAHRMFTPFGD
WFRQLNDCQVHLPGKVGSPIHKIDELCNNRMGIPALSGNTLSLQASPNEILHAIIEDIENAQTSIKMVFYIWHPGGLTDS
VASALIRAAKRGVDVKVLLDSAGSPRFFKSHWRSMMKDAGVHVVQALEVSPWRIFLRRLDLRQHRKIIVIDESIAYTGSM
NMVDPAHFKQSSGVGQWIDVMVRVTGPTVNVLSAIHGWDWEVETGERILPDLPECPVEEIMTHHPVQVVPSGPGMPEYLI
LQVLTIAINQANHSVRITTPYFVPSADLLETLKMTAQRGVSVELIIPHNNDSLMVKWASRAFYTELLEAGVKIYEFYGGL
LHTKSVVIDEEFCLIGTVNIDMRSLWLNFEVTLAVDDVHFTKQLFDLQQSYIESSHPVELEQWEQRNLRNRFFERLFYLF
NPLL
>Mature_484_residues
MEKLYHFLTLASVALYWVLVAGVTFRVVLKRRSVSVSLAWLMVIYIIPIVGVACYFLFGELNLGRKRAERAHRMFTPFGD
WFRQLNDCQVHLPGKVGSPIHKIDELCNNRMGIPALSGNTLSLQASPNEILHAIIEDIENAQTSIKMVFYIWHPGGLTDS
VASALIRAAKRGVDVKVLLDSAGSPRFFKSHWRSMMKDAGVHVVQALEVSPWRIFLRRLDLRQHRKIIVIDESIAYTGSM
NMVDPAHFKQSSGVGQWIDVMVRVTGPTVNVLSAIHGWDWEVETGERILPDLPECPVEEIMTHHPVQVVPSGPGMPEYLI
LQVLTIAINQANHSVRITTPYFVPSADLLETLKMTAQRGVSVELIIPHNNDSLMVKWASRAFYTELLEAGVKIYEFYGGL
LHTKSVVIDEEFCLIGTVNIDMRSLWLNFEVTLAVDDVHFTKQLFDLQQSYIESSHPVELEQWEQRNLRNRFFERLFYLF
NPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=490, Percent_Identity=50.8163265306122, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI1787007, Length=349, Percent_Identity=29.7994269340974, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 55105; Mature: 55105

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLYHFLTLASVALYWVLVAGVTFRVVLKRRSVSVSLAWLMVIYIIPIVGVACYFLFGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
LNLGRKRAERAHRMFTPFGDWFRQLNDCQVHLPGKVGSPIHKIDELCNNRMGIPALSGNT
CCCCHHHHHHHHHHCCCHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCEECCCE
LSLQASPNEILHAIIEDIENAQTSIKMVFYIWHPGGLTDSVASALIRAAKRGVDVKVLLD
EEEECCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEC
SAGSPRFFKSHWRSMMKDAGVHVVQALEVSPWRIFLRRLDLRQHRKIIVIDESIAYTGSM
CCCCCHHHHHHHHHHHHHCCCCEEHHHCCCHHHHHHHHHHHHHCCEEEEEECCEEECCCC
NMVDPAHFKQSSGVGQWIDVMVRVTGPTVNVLSAIHGWDWEVETGERILPDLPECPVEEI
CCCCHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHCCCCCEECCCCCCCCCCCCCCHHHH
MTHHPVQVVPSGPGMPEYLILQVLTIAINQANHSVRITTPYFVPSADLLETLKMTAQRGV
HCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCC
SVELIIPHNNDSLMVKWASRAFYTELLEAGVKIYEFYGGLLHTKSVVIDEEFCLIGTVNI
EEEEEEECCCCEEEEEEHHHHHHHHHHHCCHHHHHHHCCHHHCCEEEEECCEEEEEEEEC
DMRSLWLNFEVTLAVDDVHFTKQLFDLQQSYIESSHPVELEQWEQRNLRNRFFERLFYLF
EEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NPLL
CCCC
>Mature Secondary Structure
MEKLYHFLTLASVALYWVLVAGVTFRVVLKRRSVSVSLAWLMVIYIIPIVGVACYFLFGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
LNLGRKRAERAHRMFTPFGDWFRQLNDCQVHLPGKVGSPIHKIDELCNNRMGIPALSGNT
CCCCHHHHHHHHHHCCCHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCEECCCE
LSLQASPNEILHAIIEDIENAQTSIKMVFYIWHPGGLTDSVASALIRAAKRGVDVKVLLD
EEEECCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEC
SAGSPRFFKSHWRSMMKDAGVHVVQALEVSPWRIFLRRLDLRQHRKIIVIDESIAYTGSM
CCCCCHHHHHHHHHHHHHCCCCEEHHHCCCHHHHHHHHHHHHHCCEEEEEECCEEECCCC
NMVDPAHFKQSSGVGQWIDVMVRVTGPTVNVLSAIHGWDWEVETGERILPDLPECPVEEI
CCCCHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHCCCCCEECCCCCCCCCCCCCCHHHH
MTHHPVQVVPSGPGMPEYLILQVLTIAINQANHSVRITTPYFVPSADLLETLKMTAQRGV
HCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCC
SVELIIPHNNDSLMVKWASRAFYTELLEAGVKIYEFYGGLLHTKSVVIDEEFCLIGTVNI
EEEEEEECCCCEEEEEEHHHHHHHHHHHCCHHHHHHHCCHHHCCEEEEECCEEEEEEEEC
DMRSLWLNFEVTLAVDDVHFTKQLFDLQQSYIESSHPVELEQWEQRNLRNRFFERLFYLF
EEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NPLL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA