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The map label for this gene is intIA [H]

Identifier: 218709877

GI number: 218709877

Start: 2032812

End: 2033786

Strand: Direct

Name: intIA [H]

Synonym: VS_1890

Alternate gene names: 218709877

Gene position: 2032812-2033786 (Clockwise)

Preceding gene: 218709867

Following gene: 218709878

Centisome position: 61.61

GC content: 41.13

Gene sequence:

>975_bases
ATGCAAAAATCACCATTTCTAGCCTATATACAAGAGTATATGTTGGGGAGACACTATGCTCTTCGTACTACACAGGCGTA
CATTTATTGGATTCACCAGTACATTCTTTATCATAATAAAAAGCATCCCAGAAATTTGACGTCGAGGCATGTAGAAGACT
TCCTGACGCACTTAGTTGTTGATAGGAAGTCAGCACAGAAAACGCAGAGCCTAGCCCTCAATGCGATAGTCTTTTTATAC
AAAGAAATCATCCAGGAACCCATTGAACTTGATATGCAGTTTCGCCGTTCAGACAAATCCCGAAAGTTGCCAACGGTTAT
GACGCCAGAAGAAATTAGCAGGTTCTTTAACCACTGCTCACCTAACTTCAAATTACCTTACCAATTAATGTATGGGTCAG
GCCTACGGTTGATGGAGTGTCTACGCTTACGAATGCAAGACATCGACTATGACTACAAATCAGTGCGAGTGTGGCAAGGA
AAAGGAGGAAAGAACCGTATAGTGACAATAGCCCCCGAGCTTTTCCCTTCAATCAAGTTACAACAACAGAAATCCGCAGA
ATACTATCAGCAAGATATACATGATAAAGCTTTTTCTGGCGTATACCTGCCCGACTCTCTAGCTAAAAAATACCCGAGTG
CAGAAAGAAGCTTAAATTGGCAATTTCTTTTTCCTTCTGCTCGTCTGGCACCCGATTTGCGAACAGGTGAATTACGTAGG
CATCATATTCATCCAACAGCACTACAAAAACACATTAAGGTCGCTAGCCAAAAAGCCGACATTGAGAAAAATATTTCCTG
CCACACTTTAAGGCATTCATTTGCGACGCACCTTCTTCAAAGCGGTGCTGATATTAGAACAGTTCAAGAACAGCTTGGAC
ATACCGATCTAAAAACAACGCAAATATATACTCACATCATAGATAGAGGAGCCAACGGCGTAATTAGTCCACTATCAAAA
ATATTCGTTAAGTAA

Upstream 100 bases:

>100_bases
ACAAGCATCTGCCTGAATCCCAAAATACAGATTTTTTCTATATAGCATGAATTGATGTGCCAAACTGCAATTCATGTACA
TATAACCAGTTAAAATTAAA

Downstream 100 bases:

>100_bases
TTGTCGGGGAATCTCGGAGTCCGAAGTTTCGTAAACTTAGCGGCTAACCACTCACTTACATACCTTTACTTACCAAACCA
TGAAACTTGCTAAATTAGAT

Product: Site-specific recombinase IntIA

Products: NA

Alternate protein names: E2 protein [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MQKSPFLAYIQEYMLGRHYALRTTQAYIYWIHQYILYHNKKHPRNLTSRHVEDFLTHLVVDRKSAQKTQSLALNAIVFLY
KEIIQEPIELDMQFRRSDKSRKLPTVMTPEEISRFFNHCSPNFKLPYQLMYGSGLRLMECLRLRMQDIDYDYKSVRVWQG
KGGKNRIVTIAPELFPSIKLQQQKSAEYYQQDIHDKAFSGVYLPDSLAKKYPSAERSLNWQFLFPSARLAPDLRTGELRR
HHIHPTALQKHIKVASQKADIEKNISCHTLRHSFATHLLQSGADIRTVQEQLGHTDLKTTQIYTHIIDRGANGVISPLSK
IFVK

Sequences:

>Translated_324_residues
MQKSPFLAYIQEYMLGRHYALRTTQAYIYWIHQYILYHNKKHPRNLTSRHVEDFLTHLVVDRKSAQKTQSLALNAIVFLY
KEIIQEPIELDMQFRRSDKSRKLPTVMTPEEISRFFNHCSPNFKLPYQLMYGSGLRLMECLRLRMQDIDYDYKSVRVWQG
KGGKNRIVTIAPELFPSIKLQQQKSAEYYQQDIHDKAFSGVYLPDSLAKKYPSAERSLNWQFLFPSARLAPDLRTGELRR
HHIHPTALQKHIKVASQKADIEKNISCHTLRHSFATHLLQSGADIRTVQEQLGHTDLKTTQIYTHIIDRGANGVISPLSK
IFVK
>Mature_324_residues
MQKSPFLAYIQEYMLGRHYALRTTQAYIYWIHQYILYHNKKHPRNLTSRHVEDFLTHLVVDRKSAQKTQSLALNAIVFLY
KEIIQEPIELDMQFRRSDKSRKLPTVMTPEEISRFFNHCSPNFKLPYQLMYGSGLRLMECLRLRMQDIDYDYKSVRVWQG
KGGKNRIVTIAPELFPSIKLQQQKSAEYYQQDIHDKAFSGVYLPDSLAKKYPSAERSLNWQFLFPSARLAPDLRTGELRR
HHIHPTALQKHIKVASQKADIEKNISCHTLRHSFATHLLQSGADIRTVQEQLGHTDLKTTQIYTHIIDRGANGVISPLSK
IFVK

Specific function: Putative integrase believed to be involved in the insertion of antibiotic resistance genes into plasmids and transposons [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1789261, Length=330, Percent_Identity=29.6969696969697, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790244, Length=179, Percent_Identity=31.2849162011173, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR011946
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 37909; Mature: 37909

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKSPFLAYIQEYMLGRHYALRTTQAYIYWIHQYILYHNKKHPRNLTSRHVEDFLTHLVV
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
DRKSAQKTQSLALNAIVFLYKEIIQEPIELDMQFRRSDKSRKLPTVMTPEEISRFFNHCS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCC
PNFKLPYQLMYGSGLRLMECLRLRMQDIDYDYKSVRVWQGKGGKNRIVTIAPELFPSIKL
CCCCCCEEEECCCCHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCEEEECHHHCCCCHH
QQQKSAEYYQQDIHDKAFSGVYLPDSLAKKYPSAERSLNWQFLFPSARLAPDLRTGELRR
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHCCCEEEECCCHHCCCCCCCCCHHH
HHIHPTALQKHIKVASQKADIEKNISCHTLRHSFATHLLQSGADIRTVQEQLGHTDLKTT
HCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHH
QIYTHIIDRGANGVISPLSKIFVK
HHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MQKSPFLAYIQEYMLGRHYALRTTQAYIYWIHQYILYHNKKHPRNLTSRHVEDFLTHLVV
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
DRKSAQKTQSLALNAIVFLYKEIIQEPIELDMQFRRSDKSRKLPTVMTPEEISRFFNHCS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCC
PNFKLPYQLMYGSGLRLMECLRLRMQDIDYDYKSVRVWQGKGGKNRIVTIAPELFPSIKL
CCCCCCEEEECCCCHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCEEEECHHHCCCCHH
QQQKSAEYYQQDIHDKAFSGVYLPDSLAKKYPSAERSLNWQFLFPSARLAPDLRTGELRR
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHCCCEEEECCCHHCCCCCCCCCHHH
HHIHPTALQKHIKVASQKADIEKNISCHTLRHSFATHLLQSGADIRTVQEQLGHTDLKTT
HCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHH
QIYTHIIDRGANGVISPLSKIFVK
HHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2821509; 2823258; 3054482; 8195081; 2188588 [H]